Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 9,719 G→A 6.0% intergenic (+24/+63) gyrA → / ← nnrD DNA gyrase subunit A/ADP‑dependent (S)‑NAD(P)H‑hydrate dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE9,7190GA 6.0% 519.3 / 13.2 167intergenic (+24/+63)gyrA/nnrDDNA gyrase subunit A/ADP‑dependent (S)‑NAD(P)H‑hydrate dehydratase
Reads supporting (aligned to +/- strand):  ref base G (81/75);  new base A (4/7);  total (85/82)
Fisher's exact test for biased strand distribution p-value = 3.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01

Only 100 of 171 total aligned reads displayed.

TTCATACTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCACATACATATCTTTTGC  >  USA300TCH1516_ALE/9580‑9859
                                                                                                                                                  |                                                                                                                                             
ttCATACTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGt                                                                                                                                              <  2:476128/141‑1 (MQ=255)
    tACTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAggg                                                                                                                                          >  4:351158/1‑141 (MQ=255)
     aCTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTCCCCTACATATAggga                                                                                                                                         <  1:113654/141‑2 (MQ=255)
      cTGAAGTGATTGATTCAGAAGAAAATGATGAAGATTGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGa                                                                                                                                        <  2:296796/141‑1 (MQ=255)
      cTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATCAGATGAAGATGAAGAATAATAAAAAAATAAGACTTCCCTATATg                                                                                                                                               >  2:1061339/1‑141 (MQ=255)
          aGTGATTTATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCt                                                                                                                                    <  4:109104/141‑1 (MQ=255)
          aGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTCCCCTACATATAGGGAAGTCt                                                                                                                                    <  1:433007/141‑1 (MQ=255)
          aGTGATGGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTCCCCTACATATAGGGAAGTCt                                                                                                                                    <  2:837236/141‑1 (MQ=255)
           gTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCtt                                                                                                                                   <  1:211969/141‑1 (MQ=255)
            tgattgatTCAGAAGAAAATGATTAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTACATATAGGGAAGTCTTa                                                                                                                                  <  4:162995/141‑1 (MQ=255)
                 gattCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑ttttt                                                                                                                            <  2:283433/141‑1 (MQ=255)
                  attCATAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTa                                                                                                                           <  2:970307/141‑1 (MQ=255)
                   ttCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTAt                                                                                                                          >  1:177309/1‑141 (MQ=255)
                     cAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGc                                                                                                                        >  4:327271/1‑141 (MQ=255)
                      agaagaAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTCCCCTACATATAGGGAAGTCTTA‑ttttttatta                                                                                                                       <  1:618374/141‑6 (MQ=38)
                             aaTGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGt                                                                                                                <  3:305020/141‑1 (MQ=255)
                             aaTGATGAAGATGGACGTAAGTAATTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGt                                                                                                                <  1:283622/141‑1 (MQ=255)
                              atgatgAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTa                                                                                                               >  4:27997/1‑141 (MQ=255)
                               tgatgaAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTaa                                                                                                              <  2:990334/141‑1 (MQ=255)
                               tgatgaAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATA‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTaa                                                                                                              >  1:243254/1‑141 (MQ=255)
                                       aTGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTAc                                                                                                      <  1:210719/141‑1 (MQ=255)
                                       aTGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTAc                                                                                                      <  2:199616/141‑1 (MQ=255)
                                       aTGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTAc                                                                                                      <  4:444363/141‑1 (MQ=255)
                                         ggACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACta                                                                                                    >  1:192107/1‑141 (MQ=255)
                                         ggACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACta                                                                                                    >  1:746766/1‑141 (MQ=255)
                                             gTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATAtt                                                                                                <  4:217761/141‑1 (MQ=255)
                                                ttGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCaa                                                                                             >  3:47487/1‑141 (MQ=255)
                                                 tGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAAt                                                                                            <  2:487767/141‑1 (MQ=255)
                                                 tGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAAt                                                                                            <  2:293606/141‑1 (MQ=255)
                                                        aGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGt                                                                                     <  1:564714/141‑1 (MQ=255)
                                                         gACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTa                                                                                    >  1:100784/1‑141 (MQ=255)
                                                           cAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATCAGATGAAGATGAAGAATAATAAAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTGTACTATATTCAATGAt                                                                                         <  1:451709/141‑1 (MQ=255)
                                                             aGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGa                                                                                <  2:940476/141‑1 (MQ=255)
                                                             aGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGa                                                                                >  2:192083/1‑141 (MQ=255)
                                                             aGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TATTTATGCTAGAAAGTAATGCTTTACTATATTAAATTATTAGTAAGGa                                                                                >  4:190925/1‑141 (MQ=255)
                                                              gATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGAc                                                                               <  1:90786/141‑1 (MQ=255)
                                                               aTTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGNTTTACTATATTCNATGATTAGTAATGACt                                                                              <  1:657834/141‑1 (MQ=255)
                                                                tttCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTa                                                                             <  4:444285/141‑1 (MQ=255)
                                                                tttCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATCACTa                                                                             >  2:788497/1‑141 (MQ=255)
                                                                   cATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACt                                                                          >  3:53366/1‑141 (MQ=255)
                                                                    aTGGATCATGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACGATATTCAATGATTAGTAATGACTAACtt                                                                         >  3:402831/1‑141 (MQ=255)
                                                                     tGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACttt                                                                        >  2:181790/1‑141 (MQ=255)
                                                                     tGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACttt                                                                        >  3:122668/1‑141 (MQ=255)
                                                                     tGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACttt                                                                        >  2:20686/1‑141 (MQ=255)
                                                                     tGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACttt                                                                        >  1:177473/1‑141 (MQ=255)
                                                                       gATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCt                                                                      <  3:330685/141‑1 (MQ=255)
                                                                       gATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCt                                                                      >  1:1030712/1‑141 (MQ=255)
                                                                        aTCGTGTTGAAGAAGATATACAACAATCATG‑‑‑‑‑‑AGATGGAGAATAAT‑AAAAAATAAGACTACCCTATATGAAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTa                                                                     >  2:913163/1‑141 (MQ=255)
                                                                          ggtgtTGAAGAAAATAGAGAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGACAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAAt                                                                   <  2:415537/140‑1 (MQ=255)
                                                                            tgtTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTg                                                                 <  1:111178/141‑1 (MQ=255)
                                                                            ggtTGAAGAAGATATACATCACTCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTg                                                                 <  4:352847/140‑1 (MQ=255)
                                                                                gaagaaGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTc                                                             <  3:180200/141‑1 (MQ=255)
                                                                                     agaTATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGc                                                        >  4:336489/1‑141 (MQ=255)
                                                                                     agaTATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGc                                                        >  4:60491/1‑141 (MQ=255)
                                                                                      gaTATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCg                                                       <  2:686842/141‑1 (MQ=255)
                                                                                        tataCAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTa                                                     >  2:663054/1‑141 (MQ=255)
                                                                                           acaacaATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAgg                                                  <  1:690230/141‑1 (MQ=255)
                                                                                           acaacaATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAACAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAgg                                                  <  1:1041746/141‑1 (MQ=255)
                                                                                            caacaaTCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGt                                                 >  2:393441/1‑141 (MQ=255)
                                                                                            caacaaTCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCCTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGt                                                 >  2:499878/1‑141 (MQ=255)
                                                                                            caacaaTCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGGCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGt                                                 >  4:386869/1‑141 (MQ=255)
                                                                                              acaaTCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTAt                                               <  2:344300/141‑1 (MQ=255)
                                                                                                aaTCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTAttt                                             <  1:332201/141‑1 (MQ=255)
                                                                                                  tcatc‑‑‑‑‑‑aGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCa                                           >  2:724733/1‑141 (MQ=255)
                                                                                                  tcatc‑‑‑‑‑‑aGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCa                                           >  2:666732/1‑141 (MQ=255)
                                                                                                   catc‑‑‑‑‑‑aGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCAt                                          <  3:451077/141‑1 (MQ=255)
                                                                                                      c‑‑‑‑‑‑aGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGa                                       >  2:907586/1‑141 (MQ=255)
                                                                                                             acaTGAAGAATAAT‑AAATAATAAGACTTCCCCATATGGAGGGCAGTCTTA‑‑TTTGATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAAGTGTTTCATTGCGTAAGGTATTTCATTGATa                                     <  3:327034/139‑1 (MQ=255)
                                                                                                             aGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGAt                                      <  1:323896/141‑1 (MQ=255)
                                                                                                             aGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGAt                                      <  2:1010903/141‑1 (MQ=255)
                                                                                                                 gaagaaTAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGt                                  >  2:1067252/1‑141 (MQ=255)
                                                                                                                   agaaTAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCt                                <  1:840406/141‑1 (MQ=255)
                                                                                                                        aat‑aaAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCTTTGCGTAAGGTATTTCATTGATAGGTCTTGAtg                           >  1:682025/1‑141 (MQ=255)
                                                                                                                         at‑aaAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGAtgg                          >  1:728038/1‑141 (MQ=255)
                                                                                                                         at‑aaAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGAtgg                          <  2:717268/141‑1 (MQ=255)
                                                                                                                            aaaaaaTAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGAtggtg                        >  2:576217/1‑141 (MQ=255)
                                                                                                                             aaaaaTAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGAtggtgg                       <  1:94283/141‑1 (MQ=255)
                                                                                                                              aaaaTAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGc                      <  4:53366/141‑1 (MQ=255)
                                                                                                                               aaaTAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGca                     <  2:761296/141‑1 (MQ=255)
                                                                                                                               aaaTAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTAATGGTTGca                     >  2:890190/1‑141 (MQ=255)
                                                                                                                                aaTAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGcac                    <  3:103367/141‑1 (MQ=255)
                                                                                                                                 aTAAGACTCCCCTACATATAGGGAAGTCTTATTTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGcac                    >  1:528655/1‑141 (MQ=38)
                                                                                                                                  tAAGACTCCCCTACATATAGGGAAGTCTTATTTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGcacc                   >  3:328910/1‑141 (MQ=38)
                                                                                                                                    aGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCacaaca                >  2:804462/1‑141 (MQ=255)
                                                                                                                                    aGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACcaca                >  2:321883/1‑141 (MQ=255)
                                                                                                                                    aGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACcaca                >  1:520281/1‑141 (MQ=255)
                                                                                                                                    aGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACcaca                >  1:244719/1‑141 (MQ=255)
                                                                                                                                    aGACTCCCCTACATATAGGGAAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACcaca                >  1:451741/1‑141 (MQ=255)
                                                                                                                                     gACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCacat               >  2:455634/1‑141 (MQ=255)
                                                                                                                                      actccccTACATATAGGGAAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCacata              >  3:137706/5‑141 (MQ=255)
                                                                                                                                         tCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCacatacat           >  1:815935/1‑141 (MQ=255)
                                                                                                                                          cccTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGTCACCAcatacata          >  3:408167/1‑141 (MQ=255)
                                                                                                                                          cccTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCAcatacata          >  4:2626/1‑141 (MQ=255)
                                                                                                                                          cccTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCAcatacata          >  1:257318/1‑141 (MQ=255)
                                                                                                                                          cccTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCAcatacata          >  1:200707/1‑141 (MQ=255)
                                                                                                                                              atatGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCACATACATATCtt      >  1:708208/1‑141 (MQ=255)
                                                                                                                                              atatGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCACATACATATCtt      >  1:185172/1‑141 (MQ=255)
                                                                                                                                               tatGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCACATACATATCttt     <  1:132340/141‑1 (MQ=255)
                                                                                                                                               tatGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCACATACATATCttt     >  3:223406/1‑141 (MQ=255)
                                                                                                                                                  gTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCACATACATATCTTTTGc  >  4:203455/1‑141 (MQ=255)
                                                                                                                                                  |                                                                                                                                             
TTCATACTGAAGTGATTGATTCAGAAGAAAATGATGAAGATGGACGTATTGAAGTAAGACAAGATTTCATGGATCGTGTTGAAGAAGATATACAACAATCATC‑‑‑‑‑‑AGATGAAGAATAAT‑AAAAAATAAGACTTCCCTATATGTAGGGGAGTCTTA‑TTTTTATGCTAGAAAGTAATGCTTTACTATATTCAATGATTAGTAATGACTAACTTTCTAATTGTTTCATTGCGTAAGGTATTTCATTGATAAGTCTTGATGGTGGCACCACATACATATCTTTTGC  >  USA300TCH1516_ALE/9580‑9859

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑