BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	18,223	G→A	5.1%	V273I (GTT→ATT)	USA300HOU_RS00065 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	18,223	0	G	A	5.1%	422.7 / 6.5	132	V273I (GTT→ATT)	USA300HOU_RS00065	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (77/47); new base A (4/3); total (81/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 134 total aligned reads displayed.

GTCAACCGAGTGTGTTCCAGAGCATTCTTTTAAACTTCCAACTTGTGTTATCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAAATAATTCTAAAAAGTGAATAGAGGTGGAATAATGAATCGGCAGTCCACT  >  USA300TCH1516_ALE/18083‑18362

gTCAACCGAGTGTGTTCCAGAGCATTCTTTTAAACTTCCAACTTGTGTTATCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTgg                                                                                                                                              <  2:248855/141‑1 (MQ=255)

 tCAACCGAGTGTGTTCCAGAGCATTCTTTTAAACTTCCAACTTGTGTTATCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGt                                                                                                                                             >  3:215453/1‑141 (MQ=255)

     ccGAGTGTGTTCCATAGCAGTCGTTTAAACTTCCAACTTGTGTTATCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCTAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATc                                                                                                                                         <  4:65104/141‑1 (MQ=255)

     ccGAGTGTGTTCCAGAGCATTCTTTTAAACTTCCAACTTGTGTTATCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATc                                                                                                                                         >  1:830889/1‑141 (MQ=255)

        aGTGTGTTCCAGAGCATTCTTTTAAACTTCCAACTTGTGTTATCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGt                                                                                                                                      >  2:20457/1‑141 (MQ=255)

         gtgtgtTCCAGAGCATTCTTTTAAACTTCCAACTTGTGTTATCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGtc                                                                                                                                     >  2:7600/1‑140 (MQ=255)

         gtgtgtTCCAGAGCATTCTTTTAAACTTCCAACTTGTGTTATCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTa                                                                                                                                     >  1:598861/1‑141 (MQ=255)

         gtgtgtTCCAGAGCATTCTTTTAAACTTCCAACTTGTGTTATCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGAGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTa                                                                                                                                     >  2:741233/1‑141 (MQ=255)

           gtgtTCCAGAGCATTCTTTTAAACTTCCAACTTGTGTTATCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACa                                                                                                                                   >  2:55679/1‑141 (MQ=255)

           gtgtTCCAGAGCATTCTTTTAAACTTCCAACTTGTGTTATCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACa                                                                                                                                   <  2:1041195/141‑1 (MQ=255)

            tgtTCCAGAGCATTCTTTTAAACTTCCAACTTGTGTTATCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACaa                                                                                                                                  <  2:414334/141‑1 (MQ=255)

               tCCAGAGCATTCTTTTAAACTTCCAACTTGTGTTATCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATAc                                                                                                                               >  4:150825/1‑141 (MQ=255)

                   gagCATTCTTTTAAACTTCCAACTTGTGTTATCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGac                                                                                                                           >  3:32191/1‑141 (MQ=255)

                   gagCATTCTTTTAAACTTCCAACTTGTGTTATCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGATTATCGGTACAATACTGac                                                                                                                           >  2:622168/1‑141 (MQ=255)

                     gCATTCTTTTAAACTTCCAACTTGTGTTATCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGacac                                                                                                                         >  2:643272/1‑141 (MQ=255)

                     gCATTCTTTTAAACTTCCAACTCGTGTTATCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCTAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGacac                                                                                                                         <  3:378919/141‑1 (MQ=255)

                         tCTTTTAAACTTCCAACTTGTGTTATCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTAc                                                                                                                     >  4:449196/1‑141 (MQ=255)

                          cTTTTAAACTTCCAACTTGTGTTATCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACg                                                                                                                    <  2:440360/141‑1 (MQ=255)

                           ttttAAACTTCCAACTTGTGTTATCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGa                                                                                                                   >  3:457267/1‑141 (MQ=255)

                             ttAAACTTCCAACTTGTGTTATCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGa‑ca                                                                                                                <  3:304112/141‑1 (MQ=255)

                              tAATCTTACTACTTGTGTTATCATTAGTAATGTATATTGAATGTTTAAGTTTTATTCATTTCTTTGGTAAGGCGAAAAATTTTCCGATAGCAGTAACGATTTTACTATTGTTTATCGGTACAATATTGTCACCTTCGa‑cac                                                                                                               <  4:58162/141‑1 (MQ=255)

                              tAAACTTCCAACTTGTGTTATCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGa‑cac                                                                                                               <  2:310570/141‑1 (MQ=255)

                                     ccAACTTGTGTTATCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGt                                                                                                        >  1:393860/1‑141 (MQ=255)

                                      cAACTTGTGTTATCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTa                                                                                                       >  4:140941/1‑141 (MQ=255)

                                         cTTGTGTTATCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGa                                                                                                    <  3:395508/141‑1 (MQ=255)

                                          ttGTGTTATCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGac                                                                                                   >  1:350367/1‑141 (MQ=255)

                                          ttGTGTTATCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGac                                                                                                   >  3:104242/1‑141 (MQ=255)

                                            gtgtTATCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGacta                                                                                                 >  1:658855/1‑141 (MQ=255)

                                             tgtTATCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGactac                                                                                                >  4:291743/1‑141 (MQ=255)

                                              gtTATCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGactact                                                                                               <  2:533744/141‑1 (MQ=255)

                                              gtTATCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGactact                                                                                               >  1:144658/1‑141 (MQ=255)

                                               ttATCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAGGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGACTACtt                                                                                              >  2:372866/1‑141 (MQ=255)

                                                 aTCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGACTACTTgg                                                                                            >  2:786199/1‑141 (MQ=255)

                                                 aTCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGACTACTTgg                                                                                            <  1:190811/141‑1 (MQ=255)

                                                 aTCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGACGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATATTGACACCTACGA‑CACATATTGTATGACTACTTgg                                                                                            >  2:832848/1‑141 (MQ=255)

                                                      tAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGACTACTTGGTGTTa                                                                                       >  4:138538/1‑141 (MQ=255)

                                                      tAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGACTACTTGGTGTTa                                                                                       <  1:17225/141‑1 (MQ=255)

                                                      tAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGACTACTTGGTGTTa                                                                                       >  1:1033467/1‑141 (MQ=255)

                                                        gTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCTGTACAATACTGACACCTACGA‑CACATATTGTAGGACTACTTGGTGTTATc                                                                                     >  3:193308/1‑141 (MQ=255)

                                                        gTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGACTACTTGGTGTTATc                                                                                     >  3:62826/1‑141 (MQ=255)

                                                          aaTGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATTGGTACAATACTGACACCTACGA‑CACATATTGTAGGACTACTTGGTGTTATCGa                                                                                   <  1:550136/141‑1 (MQ=255)

                                                           aTGTATATTCAAGGGTTAAGTCTTATTCATTTGGTTGGTAAAGCGTAATTTTTGCCGAATGCAGTAACGATTTTCCTATTGGTTATCCGTACAATATTGACACCTACCA‑CACAGATCGTAGTAGTACTTGGTGatatggtt                                                                                  >  4:181211/1‑137 (MQ=255)

                                                             gTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGACTACTTGGTGTTATCGAttt                                                                                >  1:429571/1‑141 (MQ=255)

                                                             gTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGACTACTTGGTGTTATCGAttt                                                                                <  2:72291/141‑1 (MQ=255)

                                                             gTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGACTACTTGGTGTTATCGAttt                                                                                <  2:617162/141‑1 (MQ=255)

                                                              tataTTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGACTACTTGGTGTTATCGATTTa                                                                               <  1:906752/141‑1 (MQ=255)

                                                                 atTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGACTACTTGGTGTTATCGATTTAAGt                                                                            >  1:159587/1‑141 (MQ=255)

                                                                            aaGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGACTACTTGGTGTTATCGATTTAAGtttgaatttgaa                                                                 <  1:978095/141‑1 (MQ=255)

                                                                              gtgtTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGc                                                               >  3:274284/1‑141 (MQ=255)

                                                                              gtgtTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGc                                                               >  2:473027/1‑141 (MQ=255)

                                                                              gtgtTATTCAGTTCTTTGTGAAAGCGAAATGTTGGCCGAAGGCAGTGACGATTTTACTATTGGTTATCGGTACAATACGGACACCTACGA‑CACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGc                                                               <  1:217879/141‑1 (MQ=255)

                                                                               tgtTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCg                                                              <  1:205364/141‑1 (MQ=255)

                                                                                gtTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTTACTTTGAAGCGa                                                             <  2:153594/141‑1 (MQ=255)

                                                                                gtTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGa                                                             <  1:131223/141‑1 (MQ=255)

                                                                                 ttATTCATTTTTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGaa                                                            <  1:965446/141‑1 (MQ=255)

                                                                                       aTTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGa                                                      <  4:62826/141‑1 (MQ=255)

                                                                                       aTTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGa                                                      <  4:306396/141‑1 (MQ=255)

                                                                                        tttCTTTGGTAAAGCGAAAGGTTTGCAGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGACCACA‑ATTGTAGGACTACTTGGTGTTATAGATTTAAGTTTGAATTTGAAGCGAATCATGaa                                                     >  1:431645/1‑141 (MQ=255)

                                                                                         ttCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGATTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGCATCATGaaa                                                    >  1:60110/1‑141 (MQ=255)

                                                                                           cTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGaaaaa                                                  >  4:63486/1‑141 (MQ=255)

                                                                                             ttGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAaata                                                >  1:362504/1‑141 (MQ=255)

                                                                                             ttGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAaata                                                >  1:175096/1‑141 (MQ=255)

                                                                                              tGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAaataa                                               <  2:297812/141‑1 (MQ=255)

                                                                                                gTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAAATAAtt                                             <  1:538893/141‑1 (MQ=255)

                                                                                                gTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAAATAAtt                                             >  1:463672/1‑141 (MQ=255)

                                                                                                  aaaGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAAATAATTCt                                           <  2:779802/141‑1 (MQ=255)

                                                                                                  aaaGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAAATAATTCt                                           <  3:367531/141‑1 (MQ=255)

                                                                                                   aaGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGACGCGAATCATGAAAaataaatata                                          <  2:185965/141‑6 (MQ=255)

                                                                                                   aaGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAAATAATTCTa                                          >  2:668780/1‑141 (MQ=255)

                                                                                                   aaGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAAATAATTCTa                                          >  4:124807/1‑141 (MQ=255)

                                                                                                    aGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAAATAATTCTaa                                         <  3:136712/141‑1 (MQ=255)

                                                                                                    aGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAAATAATTCTaa                                         >  4:39473/1‑141 (MQ=255)

                                                                                                      cGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAAATAATTCTaaaa                                       <  2:892911/141‑1 (MQ=255)

                                                                                                        aaaGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAAATAATTCTAAAAAg                                     <  1:741233/141‑1 (MQ=255)

                                                                                                            gTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAAATAATTCTAAAAAGTGaa                                 <  2:727931/141‑1 (MQ=255)

                                                                                                              ttGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAAATAATTCTAAAAAGTGAATa                               <  1:740785/141‑1 (MQ=255)

                                                                                                              ttGCCGAATGCAGTAACGATTTTACTATTGATTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAAATAATTCTAAAAAGTGAATa                               <  1:1040818/141‑1 (MQ=255)

                                                                                                                 ccGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAAAAAATTATAAAAAGTGAATagag                            <  2:595230/141‑1 (MQ=255)

                                                                                                                 ccGAATGCAGTAACGATTTTACTATTGATTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAAATAATTCTAAAAAGTGAATagag                            >  2:97677/1‑141 (MQ=255)

                                                                                                                     aTGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAAATAATTCTAAAAAGTGAATAGAGGTgg                        >  1:666672/1‑141 (MQ=255)

                                                                                                                      tGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAAATAATTCTAAAAAGTGAATAGAGGTGGa                       <  1:1043999/141‑1 (MQ=255)

                                                                                                                         attAACGATGTTACTATTGGTTAGCGGTACAATACTGACACCTACGT‑CACATATTGTAGGACTACTTGTTGTTATCGATTTAAGTTTTAATTTGAAGCGAATCATGAAAAATAATTCTAAAAAGTGAATAGAGGTTGaata                    <  4:246396/139‑1 (MQ=255)

                                                                                                                         aGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAAATAATTCTAAAAAGTGAATAGAGGTGGaata                    <  1:254425/141‑1 (MQ=255)

                                                                                                                          gTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAAATAATTCTAAAAAGTGAATAGAGGTGGaataa                   >  2:619250/1‑141 (MQ=255)

                                                                                                                          gTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAAATAATTCTAAAAAGTGAATAGAGGTGGaataa                   >  2:1054389/1‑141 (MQ=255)

                                                                                                                              cGATTTTACTATTGGTTATCTGTACAATACTGACACCTACGA‑CACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAAATAATTCTAAAAAGTGAATAGAGGTGGAATAATGaa               <  4:193308/141‑1 (MQ=255)

                                                                                                                                aTTTTACTATTGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAAATAATTCTAAAAAGTGAATAGAGGTGGAATAATGAATc             <  2:666672/141‑1 (MQ=255)

                                                                                                                                 ttttACTATTGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAAATAATTCTAAAAAGTGAATAGAGGTGGAATAATGAATCg            >  2:13943/1‑141 (MQ=255)

                                                                                                                                 ttttACTATTGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAAATAATTCTAAAAAGTGAATAGAGGTGGAATAATGAATCg            >  1:664873/1‑141 (MQ=255)

                                                                                                                                 ttttACTATTGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAAATAATTCTAAAAAGTGAATAGAGGTGGAATAATGAATCg            >  2:946878/1‑141 (MQ=255)

                                                                                                                                  tttACTATTGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAAATAATTCTAAAAAGTGAATAGAGGTGGAATAATGAATCgg           >  2:171408/1‑141 (MQ=255)

                                                                                                                                  tttACTATTGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAAATAATTCTAAAAAGTGAATAGAGGTGGAATAATGAATCgg           >  1:59596/1‑141 (MQ=255)

                                                                                                                                  tttACTATTGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGACTACTGGGTGTTATCGATTTCAGTTTGAATTTGAAGCGAATCATGAAAAATAATTCTAAAAAGTGAATAGAGGTGGAATAATGAATCgg           >  4:70244/1‑141 (MQ=255)

                                                                                                                                   ttACTATTGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAAATAATTCTAAAAAGTGAATAGAGGTGGAATAATGAATCGGc          >  2:491570/1‑141 (MQ=255)

                                                                                                                                   ttACTATTGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAAATAATTCTAAAAAGTGAATAGAGGTGGAATAATGAATCGGc          >  1:341728/1‑141 (MQ=255)

                                                                                                                                    tACTATTGGTTATCGGTACAATACTGACACCTACGA‑CAAATAATGTCGGAGCCCTTGGTGTTTACGACTGCAGTTTGCCGTTGAAGCTAATCATGAAAAATAATTCTAAAAAGTGAATAGAGGTGGAATAATGAATCGGCa         <  1:918444/141‑1 (MQ=255)

                                                                                                                                      cTATTGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAAATAATTCTAAAAAGTGAATAGAGGTGGAATAATGAATCGGCAGt       >  3:384222/1‑141 (MQ=255)

                                                                                                                                        aTTGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGACAACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAAATAATTCTAAAAAGTGAATAGAGGTGGAATAATGAATCGGCAGTcc     >  3:208201/1‑141 (MQ=255)

                                                                                                                                         ttGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCACGAAAAATAATTCTAAAAAGTGAATAGAGGTGGAATAATGAATCGGCAGTCCa    >  2:232884/1‑141 (MQ=255)

                                                                                                                                          tGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAAATAATTCTAAAAAGTGAATAGAGGTGGAATAATGAATCGGCAGTCCAc   >  4:368258/1‑141 (MQ=255)

GTCAACCGAGTGTGTTCCAGAGCATTCTTTTAAACTTCCAACTTGTGTTATCATTAGTAATGTATATTCAAGGTTTAAGTGTTATTCATTTCTTTGGTAAAGCGAAAGGTTTGCCGAATGCAGTAACGATTTTACTATTGGTTATCGGTACAATACTGACACCTACGA‑CACATATTGTAGGACTACTTGGTGTTATCGATTTAAGTTTGAATTTGAAGCGAATCATGAAAAATAATTCTAAAAAGTGAATAGAGGTGGAATAATGAATCGGCAGTCCACT  >  USA300TCH1516_ALE/18083‑18362

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`