BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	USA300TCH1516_ALE	2,026,955	G→A	T331I (ACA→ATA)	vraS ←	Sensor protein VraS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,026,955	0	G	A	99.1%	360.6 / NA	106	T331I (ACA→ATA)	vraS	Sensor protein VraS
Reads supporting (aligned to +/- strand): ref base G (0/1); major base A (61/43); minor base C (1/0); total (62/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 106 total aligned reads displayed.

CCAACTACTTCAATATCACTTTGCGTTGATAGATAACTTGAAATTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTGTACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTACCATTATCTTGAATTCT  >  USA300TCH1516_ALE/2026819‑2027088

ccAACTACTTCAATATCACTTTGCGTTGATAGATAACTTGAAATTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATAcc                                                                                                                                    >  2:777880/1‑141 (MQ=255)

ccAACTACTTCAATATCACTTTGCGTTGATAGATAACTTGAAATTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATAcc                                                                                                                                    <  1:87692/141‑1 (MQ=255)

   actactTCAATATCACTTTGCGTTGATAGATAACTTGAAATTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGa                                                                                                                                 <  3:20455/141‑1 (MQ=255)

     tactTCAATATCACTTTGCGTTGATAGATAACTTGAAATTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAAt                                                                                                                               >  1:531983/1‑141 (MQ=255)

       ctTCAATATCACTTTGCGTTGATAGATAACTTGAAATTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCt                                                                                                                             >  2:425034/1‑141 (MQ=255)

         tCAATATCACTTTGCGTTGATAGATAACTTGAAATTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTgg                                                                                                                           <  4:170541/141‑1 (MQ=255)

         tCAATATCACTTTGCGTTGATAGATAACTTGAAATTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTgg                                                                                                                           >  3:205686/1‑141 (MQ=255)

                cACTTTGCGTTGATAGATAACTTGAAATTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGAt                                                                                                                    <  2:660373/141‑1 (MQ=255)

                 aCTTTGCGTTGATAGATAACTTGAAATTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTACGGGTGCTTTCCCCTCGATACGTATACCTGAATCTTGCAAGGATa                                                                                                                   >  1:430262/1‑141 (MQ=255)

                 aCTTTGCGTTGATAGATAACTTGAAATTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATa                                                                                                                   >  1:478531/1‑141 (MQ=255)

                   tttGCGTTGATAGATAACTTGAAATTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACa                                                                                                                 >  1:445397/1‑141 (MQ=255)

                        gTTGATAGATAACTTGAAATTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATg                                                                                                            >  3:158931/1‑141 (MQ=255)

                        gTTGATAGATAACTTGAAATTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATg                                                                                                            >  3:99014/1‑141 (MQ=255)

                         ttGATAGATAACTTGAAATTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATgg                                                                                                           <  3:273736/141‑1 (MQ=255)

                           gatagataACTTGAAATTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑aa                                                                                                        >  1:111477/1‑141 (MQ=255)

                            atagataACTTGAAATTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AAc                                                                                                       >  2:700556/1‑141 (MQ=255)

                              agataACTTGAAATTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGt                                                                                                     <  2:739762/141‑1 (MQ=255)

                                  aaCTTGAAATTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCa                                                                                                 <  4:198803/141‑1 (MQ=255)

                                   aCTTGAAATTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCAc                                                                                                <  2:291218/141‑1 (MQ=255)

                                     ttGAAATTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCa                                                                                              >  3:157810/1‑141 (MQ=255)

                                        aaaTTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAAtt                                                                                           >  1:522806/1‑141 (MQ=255)

                                        aaaTTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGAAAC‑ATCTGGTAACGTTGCACCAAtt                                                                                           >  3:177937/1‑141 (MQ=255)

                                         aaTTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAAttt                                                                                          >  1:315458/1‑141 (MQ=255)

                                         aaTTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAAttt                                                                                          >  3:153670/1‑141 (MQ=255)

                                         aaTTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAAttt                                                                                          >  2:132000/1‑141 (MQ=255)

                                          aTTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTc                                                                                         >  1:477972/1‑141 (MQ=255)

                                             ccTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCaa                                                                                      <  2:315757/141‑1 (MQ=255)

                                             ccTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCaa                                                                                      >  1:285617/1‑141 (MQ=255)

                                             ccTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTAGTTAAAGTTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCaa                                                                                      >  1:358469/1‑141 (MQ=255)

                                              cTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCaaa                                                                                     >  2:514053/1‑141 (MQ=255)

                                              cTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCaaa                                                                                     >  1:40740/1‑141 (MQ=255)

                                               tataCGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAg                                                                                    <  2:511295/141‑1 (MQ=255)

                                               tataCGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAg                                                                                    <  1:255135/141‑1 (MQ=255)

                                               tataCGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAg                                                                                    <  1:485622/141‑1 (MQ=255)

                                                   cGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCt                                                                                                                                   >  3:94999/1‑91 (MQ=255)

                                                       ccATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTTATGTTGTTCTGGCCCGGATTCGGATGCCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCa                                                                            >  3:155659/1‑141 (MQ=255)

                                                       ccATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCa                                                                            >  1:59890/1‑141 (MQ=255)

                                                       ccATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCa                                                                            >  3:321891/1‑141 (MQ=255)

                                                        cATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCAc                                                                           <  2:323809/141‑1 (MQ=255)

                                                         aTTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACg                                                                          <  3:66523/141‑1 (MQ=255)

                                                          tttCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTg                                                                                                                                  >  1:161343/1‑85 (MQ=255)

                                                          tttCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTg                                                                                                                                  <  2:161343/85‑1 (MQ=255)

                                                           ttCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACGCa                                                                        <  1:761149/141‑1 (MQ=255)

                                                            tCATGATCATCCACACACACTACTTTGCTCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATCACTGAACCTGGCATTGCTACAATATGG‑AACGCTGCACCAATTTCCACAGCTCTTTCACGCat                                                                       <  2:276551/141‑1 (MQ=255)

                                                             cATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGa                                                                                                                                 <  2:740834/83‑1 (MQ=255)

                                                             cATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGa                                                                                                                                 >  1:740834/1‑83 (MQ=255)

                                                             cATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACGCata                                                                      >  1:691759/1‑141 (MQ=255)

                                                             cATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACATCGATACGTATACCTGAATCTGGCATTGATACAATATGG‑ATCGTTGCACCAATTTCTAAAGCTCTTTCACGCcta                                                                      >  3:286785/1‑141 (MQ=255)

                                                             cATGATCATCCACAAACAATACTCTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACGCata                                                                      >  2:124514/1‑141 (MQ=255)

                                                              aTGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACGCatat                                                                     >  3:278020/1‑141 (MQ=255)

                                                               tGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTGTACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACGCATAtt                                                                    <  1:712085/141‑1 (MQ=255)

                                                                   catcCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTa                                                                >  1:144176/1‑141 (MQ=255)

                                                                   catcCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTa                                                                >  1:605464/1‑141 (MQ=255)

                                                                        acaaacaaTACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTcc                                                           <  2:21438/141‑1 (MQ=255)

                                                                        acaaacaaTACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTcc                                                           <  2:21612/141‑1 (MQ=255)

                                                                        acaaacaaTACCTTACTCGTCATACGAATCATCATTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTcc                                                           <  2:157724/141‑1 (MQ=255)

                                                                             caaTACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAAc                                                      >  1:460715/1‑141 (MQ=255)

                                                                             caaTACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAAc                                                      >  1:387847/1‑141 (MQ=255)

                                                                               aTACTTTAATCGTCATACGAAGCCTCCTTATTTAAAGTTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACtt                                                    <  2:669665/141‑1 (MQ=255)

                                                                                tACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACGCa                                                                        <  2:129752/120‑1 (MQ=255)

                                                                                tACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACGCa                                                                        >  1:129752/1‑120 (MQ=255)

                                                                                 aCTTTAATAGACATACGAATCCTCCTTATTTAAAGGTGATTTCACATCGCTCCGTCTACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCAATCACttta                                                  >  4:293379/1‑140 (MQ=255)

                                                                                   tttAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGtt                                                <  2:429261/141‑1 (MQ=255)

                                                                                    ttAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTc                                               <  2:87357/141‑1 (MQ=255)

                                                                                      aaTCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTa                                             >  1:681542/1‑141 (MQ=255)

                                                                                       aTCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTaa                                            >  3:254755/1‑141 (MQ=255)

                                                                                       aTCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTaa                                            <  2:267330/141‑1 (MQ=255)

                                                                                           tCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAAtttt                                        <  1:769126/141‑1 (MQ=255)

                                                                                            cATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAAttttt                                       >  2:476277/1‑141 (MQ=255)

                                                                                            cATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAAttttt                                       >  1:167097/1‑141 (MQ=255)

                                                                                            cATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAAttttt                                       >  1:56688/1‑141 (MQ=255)

                                                                                            cATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAAttttt                                       >  3:10628/1‑141 (MQ=255)

                                                                                            cATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAAttttt                                       >  2:308648/1‑141 (MQ=255)

                                                                                            cATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCAAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAAttttt                                       >  1:356524/1‑141 (MQ=255)

                                                                                             aTACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTtc                                      <  1:582320/141‑1 (MQ=255)

                                                                                              tACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑ACCGTTGCCAGAGTATGACAAGATCTTTTAACTGTTTTTTTAAGTCCATAACTTTCTTCTAATTTTtca                                     >  1:667551/1‑141 (MQ=255)

                                                                                               aCGAATCCTCCTTATTTAAAGGTGCTTTCACGTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACGCATCTTTTTAAGTCCATAACTTTGTTCTAATTTTtcaa                                    >  4:174479/1‑140 (MQ=255)

                                                                                               aCGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACACTATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTtcat                                    <  1:32695/141‑1 (MQ=255)

                                                                                                cGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTtcatc                                   >  2:501647/1‑141 (MQ=255)

                                                                                                cGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTtcatc                                   <  1:514053/141‑1 (MQ=255)

                                                                                                  agtcctccTTGTTTAAATGTGCGTTCTCCTCGATACGTATACCTGAATCGCGAAATGATACAATATGG‑AACCTTGCACCAATTTCCCAAGCCCTTTCACGCATATTTTTAAGTCCATAACTATTTTCTAATTTTtcatcca                                 <  4:137103/139‑3 (MQ=255)

                                                                                                   aTCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAAc                                <  1:147339/141‑1 (MQ=255)

                                                                                                        cctTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTaa                           <  3:342059/141‑1 (MQ=255)

                                                                                                         ctTATTTAAAGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTaaa                          <  1:425034/141‑1 (MQ=255)

                                                                                                                aaaGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTa                   >  3:58008/1‑141 (MQ=255)

                                                                                                                aaaGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTa                   >  1:225127/1‑141 (MQ=255)

                                                                                                                aaaGGTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTa                   >  1:100224/1‑141 (MQ=255)

                                                                                                                    gTGCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTACCAt               <  1:476277/141‑1 (MQ=255)

                                                                                                                      gCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCaa                                 <  2:393204/121‑1 (MQ=255)

                                                                                                                      gCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTACCATTa             >  1:316195/1‑141 (MQ=255)

                                                                                                                      gCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTACCATTa             >  2:185116/1‑141 (MQ=255)

                                                                                                                      gCTTTCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTACCATTa             >  3:14856/1‑141 (MQ=255)

                                                                                                                         ttCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTACCATTATCt          <  1:315123/141‑1 (MQ=255)

                                                                                                                         ttCACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTACCATTATCt          <  2:527779/141‑1 (MQ=255)

                                                                                                                          ccACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTACCATTATCtt         <  2:430262/140‑1 (MQ=255)

                                                                                                                           cACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTACCATTATCTTg        <  1:423367/141‑1 (MQ=255)

                                                                                                                           cACCTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTACCATTATCTTg        <  2:285617/141‑1 (MQ=255)

                                                                                                                             ccTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTACCATTATCTTGaa      >  2:555767/1‑141 (MQ=255)

                                                                                                                             ccTCGATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTACCATTATCTTGaa      >  2:684302/1‑141 (MQ=255)

                                                                                                                                 gATACGTATACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTACCATTATCTTGAATtct  >  4:266706/1‑141 (MQ=255)

CCAACTACTTCAATATCACTTTGCGTTGATAGATAACTTGAAATTCCTATACGTACCATTTCATGATCATCCACAAACAATACTTTAATCGTCATACGAATCCTCCTTATTTAAAGGTGCTTTCACCTCGATACGTGTACCTGAATCTGGCAATGATACAATATGG‑AACGTTGCACCAATTTCCAAAGCTCTTTCACGCATATTTTTAAGTCCATAACTTTGTTCTAATTTTTCATCAACATTAAAACCTTTACCATTATCTTGAATTCT  >  USA300TCH1516_ALE/2026819‑2027088

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`