| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 622,464 | G→A | 11.2% | S1009S (TCG→TCA) | sdrE → | Serine‑aspartate repeat‑containing protein E |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 622,464 | 0 | G | A | 11.2% | 201.5 / 5.1 | 100 | S1009S (TCG→TCA) | sdrE | Serine‑aspartate repeat‑containing protein E |
| Reads supporting (aligned to +/- strand): ref base G (29/55); new base A (7/5); total (36/64) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.25e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-01 | |||||||||||
Only 100 of 101 total aligned reads displayed.
AGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGAT‑TCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGA > USA300TCH1516_ALE/622326‑622604 | aggcagTGATTCTGATTCAGAGAGCGACACAGATTCAGATAGCGACACA‑ACTTGGAGTTAGATCTGAGACTCAGCGGTCGATTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGa < 2:220884/138‑1 (MQ=2)agacagTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGAACCGGTCATTGAT‑TCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGa < 1:751838/141‑1 (MQ=17) cagattcagaCAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGAT‑TCAGACTCAGATAGCGACTCAGAGTCAGATAGCGATGCAGACTCAGAGAGCGACACAGATTCAGAAAGCCAGTCGGAGTCAGagcgcg > 2:343150/1‑136 (MQ=14) gattcagaCAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGAC‑TCAGACTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCCGACAGCGACTCAGACACAGAAAACGATACAGACCCacaccacgaa > 2:476470/1‑135 (MQ=11) attcagaCAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGAT‑TCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGAtt < 1:586629/141‑1 (MQ=255) gaCAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGAT‑TCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑c < 1:125487/141‑1 (MQ=255) gaCAGCGACTCAGATTAAGATAGAGACTAAGATTCGGACAGCGAT‑TCAGACTGAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑c < 2:716602/141‑1 (MQ=255) cAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGAT‑TCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTc < 1:625196/141‑1 (MQ=255) aGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGAC‑TCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACACAGATAGCGACTCAGATTCAGAGAGCGATTCGGCCTCAGATAGCGACTCAGA‑GTCa > 2:681030/1‑141 (MQ=11) gACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGAT‑TCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCGGAt < 1:994272/141‑1 (MQ=255) tccacattcagatGGCGACTCAGATGCGGACAGCGAT‑TCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAg < 2:857868/136‑1 (MQ=255) gccagatgcagaaAGCGAAGCAGATTCAGACAGCGAC‑GGAGATTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAg < 2:336126/139‑1 (MQ=11) cTCAGATTCAGATAGCGACTCAGATTCGGACAGCGAT‑TCAGACTCAGATAGCGACTCATATTCATATAGCGATTCAGACTCAGACAGCGACTCAGGTTTAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAg < 1:268216/141‑1 (MQ=255) cTCAGATTCAGATAGCGACTCAGATTCAGACAGCGAT‑TCAGTCTCAGATAGCGACGCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAg < 1:482098/141‑1 (MQ=255) cagattcagatAGCGACTCAGATTCGGACAGCGAT‑TCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCg > 1:42712/1‑141 (MQ=255) gattcagatAGCGACTCAGATTCGGACAGCGAT‑TCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCgac < 1:317524/141‑1 (MQ=255) ttcagatAGCGACTCAGATTCGGACAGCGAT‑TCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCgactc < 1:793090/141‑1 (MQ=255) ttcagatAGCGACGCAGATTCGGACAGCGAT‑TCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCgactc < 1:476170/141‑1 (MQ=255) cagatAGCGACTCAGATTCGGACAGCGAT‑TCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCgactcag > 1:599871/1‑141 (MQ=255) cagatACCGACTCAGATTCGGACAGCGAT‑TCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCgactcag < 1:221916/141‑1 (MQ=255) gatAGCGACTCAGATTCGGACAGCGAT‑TCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCgactcagac < 1:985035/141‑1 (MQ=255) atAGCGACTCAGAATCGGACAGCGAT‑TCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCgactcagact < 1:744533/141‑1 (MQ=255) aGCGACTCAGATTCGGACAGCGAT‑TCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCgactcagactca < 1:392753/141‑1 (MQ=255) aGCGACTCAGATTCGGACAGCGAT‑TCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCgactcagactca < 1:391908/141‑1 (MQ=255) aGCGACTCAGATTCGGACAGCGAT‑TCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCgactcagactca < 1:680143/141‑1 (MQ=255) gCGACTCAGATTCGGACAGCGAT‑TCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCGGACACAGACAGCGATACAGA‑GTCCGACAGCGacgcagactcag > 2:523573/1‑141 (MQ=11) gCGACTCAGATTCGGACAGCGAT‑TCAGACTCAGATAGCGACTCAGATACAGATAGCGATTCAGACTCAGACAGCGACTCAGAGTCAGATAGCGATGCGGACTCAGACAGCGATTCAGA‑CTCAGAGAGCGacccagacccag > 2:882055/1‑141 (MQ=14) aacAGATGCTGACGGCGAT‑GCAGATGCAGATAGCGACGTAGATGCAGATAGCGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAg < 2:325433/139‑1 (MQ=14) tCAGATTCGGACAGCGAT‑TCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCAGACTCAGACAGCGACTCAGA‑CTCAGATACCGACTCAGACTcagccagc > 2:312658/1‑141 (MQ=14) acAGATGCGGACAGCGAG‑GCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGc < 2:162443/140‑1 (MQ=255) cAGATTCGGACAGCGAT‑CCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGAGTCAGATAGCGATTCGGACTCAGACAGCGCTTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCg < 1:201055/141‑1 (MQ=255) aaagatagACATCGAA‑TGAGACTCAGGTAGCGACTCAGATTAAGATATCGAGTGAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGa < 2:454837/134‑1 (MQ=14) gcttCGGACAGCGAC‑TCCGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGAc < 2:649424/139‑1 (MQ=255) gactCGGACAGCGAT‑TCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGAc < 1:331411/138‑1 (MQ=255) gATTCGGACAGCGAT‑TCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATCGCGCCTCGGACTCAGACAGCGAc > 1:685676/1‑141 (MQ=255) gATTCGGACAGCGAT‑TCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGAc < 1:716282/141‑1 (MQ=255) gATTCGGACAGCGAT‑TCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGAc < 1:736508/141‑1 (MQ=255) atccagACAGCGAC‑TCAGACGCAGATAGCGACTCAGACTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACt < 2:867018/136‑1 (MQ=14) ttCAGACAGCCAT‑TTATACTGAGATAGAGACTACGATGCAGACAGCGAATCAGAGTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACtc < 2:676338/141‑1 (MQ=11) cGGACAGCGAT‑TCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACtcag > 1:367886/1‑141 (MQ=255) cGGACAGCGAT‑TCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACtcag < 1:565684/141‑1 (MQ=255) ggAGAGCTAC‑TCATACTCATAAAGCGACGCTGTGTCTGACATCGTTTCATACTCTGACAGCTACTCGGATTCAGATAGCGATTCCGACTCAGACAATG‑TTAATACCGCTTATAGCGACTCAGACTCAGACAGCGACtcaga < 2:470845/141‑1 (MQ=9) gCGAT‑TCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCCGACAGCGACTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACtcagattcag > 2:118877/1‑141 (MQ=255) ggACTCAGACAGTGACTCGGACTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGACAGCGATTCGGACTCAGACAGCGATTCAGA‑TTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGa < 2:1023471/140‑1 (MQ=11) gACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACACAGACTCAGACAGCGACTCAGATTCAGATAGCGAt > 1:950053/1‑141 (MQ=255) aCTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGaat > 1:442021/1‑139 (MQ=255) aCTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGAtt > 1:768438/1‑141 (MQ=255) tCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCg > 1:72218/1‑141 (MQ=255) tCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGATTCAGA‑CTCAGATAACGACTCACACTCAGACAGCGACTAAGATGACGATAGCGACTcc > 2:853115/1‑140 (MQ=14) tCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGCGAATca > 2:961811/1‑140 (MQ=255) aaGATAGCGACTCAGAGTCAGAGAGCGATTCAGACGCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCgg < 1:382277/140‑1 (MQ=255) aGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGA‑CTCAGATAACGACTCAGACACAAACAACGACTCAGACTCAGAAACCAACTCGGACtaa > 2:365144/1‑139 (MQ=11) cGACTCAGATTCAGATAGCGAGTAAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCAGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCACATCCACATAGCAACTCACACTCaaa > 2:751029/1‑139 (MQ=11) gACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCAGACTCAGACAGCGACTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGCATCACACTCTGACAGCGACTCGGCCTCacaa > 2:60217/1‑138 (MQ=11) tcagattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGc < 1:553723/141‑1 (MQ=255) cagattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCg > 1:931525/1‑141 (MQ=255) cagattaagatAGCGATTCAGACTCAGACAGCGACTCAGATGCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCg < 1:132891/141‑1 (MQ=255) agattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGACTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCCGACAGCGa > 2:337617/1‑141 (MQ=11) cagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGACTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGAGATACAGACTCAGACAGCGACTCaa > 2:885428/1‑140 (MQ=11) cagagAGGGATTCAGCATCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGAGAGCGACTCAGATTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAg < 2:42712/141‑1 (MQ=14) agatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGa < 1:687000/141‑1 (MQ=255) gatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACCCAGaa > 2:754532/1‑140 (MQ=14) agggaatcagactgagatAGAGACTGATATTAAGACAGCGATAGGGAATCAGAGAGCGAGTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCa < 2:284120/135‑1 (MQ=14) aGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGACAGCGATTCAGACTCAGACAGCGCCTCAGacaca > 2:333866/1‑137 (MQ=14) aTTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACa < 1:735621/141‑1 (MQ=255) ttCAGTCCCAGACCGCGACTCAGCTTCAGATAGCGATTCGGACTCAGACAGCGAGTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAg < 2:599871/141‑1 (MQ=14) ggactcagacACAGCCTCAGATTCAGAGAGCGATTCTGACTCAGACGGGGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGa < 2:830757/140‑1 (MQ=14) gactcaggtCGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGAt < 2:148919/141‑1 (MQ=255) gacgcagaaGGCGACTACGATTCAGACAGCGCTTCAGACTCAGAAAGCGACTCAGA‑CTCAGACAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGAt < 2:157069/141‑1 (MQ=11) actcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGAtt > 1:557388/1‑141 (MQ=255) actcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGACTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTTAGATAGCGACTCAGAGTCGGTCAGCGagt > 1:674402/1‑139 (MQ=14) ccagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCa < 1:950273/140‑1 (MQ=255) aaacAGCGACTCAGATTCAGATAGCGACTCGGACTCAGAGAGCGACTCAGA‑CTCAGACAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGa < 2:177145/139‑1 (MQ=255) gacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGAc < 1:939196/141‑1 (MQ=255) gacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGAc < 1:882055/141‑1 (MQ=255) cAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTc < 1:343150/141‑1 (MQ=255) gACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGAAACCAAAGACTAAATCGTTTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGAt > 1:751545/1‑141 (MQ=17) cTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGCTAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAg < 1:541075/141‑1 (MQ=255) cTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAg < 1:814072/141‑1 (MQ=255) cTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAg < 1:559104/141‑1 (MQ=255) cTCAGATTCAAATAGCAATTCGGACTCAGACAGCGATTCCGA‑CTCAGAGAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAg < 1:968881/141‑1 (MQ=255) agattcagatATCTATTCGGCCTCAGACGCCGATTCAGA‑CTCAGATAGCGCCTCAGACTCAGTCACCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCga < 1:285019/141‑1 (MQ=17) gatgcagacAGCGACGCCGACGCAGACAGCGATTCCGA‑GTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGAAAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgac < 2:472632/141‑1 (MQ=14) ttcatatCGCGATTCGGACTCAGACGGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactc < 1:493088/141‑1 (MQ=255) ttcagacAACGAGGCAGACACAGACAGCGATTCAGA‑CACAGATAGCGACTCAGAGTCAGACAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactc < 2:454050/141‑1 (MQ=11) cagatAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTAAGACTCAGATAGCgactcag > 1:868960/1‑141 (MQ=255) gatGGCGATTCAGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcagac < 1:70248/141‑1 (MQ=255) gatAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcagac < 1:392453/141‑1 (MQ=255) gatAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAAACAGCGAATCATGCTCTGATAGCcactcagac > 1:139357/1‑141 (MQ=17) gatAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGACAGCGACTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACCCCGAGCGCgactcacac > 2:244965/1‑141 (MQ=14) ttgagATTCCGGCTCAGACAGCGACCCAGA‑CTCAGACAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGATTCGGGCTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACCCAAAGATCtactcagactc < 2:376887/137‑1 (MQ=11) tAGAGATGCGGGCTAAGACGGCGACCCAGA‑CGCAGACAGCGACTCAGACTCAGACAGCGACTCAGATTCAGAAAGCGATTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcagactc < 2:804897/141‑1 (MQ=11) caGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcagactc < 1:515599/140‑1 (MQ=255) aaGCGAGTCGGACTCAGACAGGGAGTGAGA‑CTCAGATAGCGACTCACACCCAGAAAGACACTCAGACTCAGACAGCGACTCTGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcagactc < 2:592160/140‑1 (MQ=11) ttCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGAACCGGACTCAGATAGCGACTCAGATTCAGACAGCGACTCACACTACGATAGCGCCTCAGACCCAGACAg > 2:559824/1‑141 (MQ=14) tCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGt < 1:289001/141‑1 (MQ=255) cGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTg > 1:683242/1‑141 (MQ=255) cGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTg > 1:626692/1‑141 (MQ=255) cGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACCCAGACAGTg > 1:728459/1‑141 (MQ=255) ggACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGACAGCGACTCGGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTga > 2:681404/1‑141 (MQ=255) | AGACAGTGATTCAGATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGAT‑TCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGA‑CTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGA > USA300TCH1516_ALE/622326‑622604 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |