BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,774,969	A→G	9.4%	D739D (GAT→GAC)	clfB ←	Clumping factor B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,774,969	0	A	G	9.4%	264.3 / 4.9	120	D739D (GAT→GAC)	clfB	Clumping factor B
Reads supporting (aligned to +/- strand): ref base A (51/50); new base G (2/10); total (53/67)
Fisher's exact test for biased strand distribution p-value = 3.29e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 122 total aligned reads displayed.

CTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAA  >  USA300TCH1516_ALE/2774829‑2775107

ttGTCTGAGTCTGAGTCGCTGTCTGAAGCCGAATCGGTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGaa                                                                                                                                             <  2:465320/140‑1 (MQ=11)

ctttctgaatctgaaTCACTGTCTGAGTTCTATTCGTTATTTGATTCTGCATCGCTATCTGAGTTTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAGTCCGAGTCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGaa                                                                                                                                             <  2:118208/138‑1 (MQ=11)

 tGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAAt                                                                                                                                            <  1:777110/141‑1 (MQ=255)

   tctgaatctgaaTCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTTAGTCTGAAGCg                                                                                                                                          >  2:155770/1‑141 (MQ=255)

     tgaatctgaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCTGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTATGAATCCGAATCGCTATATGACTCTGACTCGATATCTGAGTATGAATCCCt                                                                                                                                        >  2:277265/1‑141 (MQ=14)

     tgaatctgaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCt                                                                                                                                        <  1:857133/141‑1 (MQ=255)

     tgaatctgaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCt                                                                                                                                        <  1:988220/141‑1 (MQ=255)

     tgaatctgaagtCCTGTCTGAATCCGACTCGCGATCTGAATCTGAAGCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGCATCTGAGTCGCTGTCTGAGTCTGAATCGCt                                                                                                                                        <  1:6658/141‑1 (MQ=17)

        ttcttaatcATTTTCTTAGTCTGAATCTCTATCTGTATCTGAATCTCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatc                                                                                                                                     <  2:309440/140‑1 (MQ=14)

        atctgaatcACTGTCTGAATCCGAATCGCTATCTGAATCCGAATCGCCATCCTAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatc                                                                                                                                     <  1:702813/141‑1 (MQ=255)

             tgtcACTATCGGAGTCCGAGTCGCTATCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaat                                                                                                                                <  2:985523/139‑1 (MQ=11)

              atcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaatc                                                                                                                               >  1:672009/1‑141 (MQ=255)

                cACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTctctgagtacg                                                                                                                             >  2:578390/1‑136 (MQ=255)

                cACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaatctg                                                                                                                             >  1:726990/1‑141 (MQ=255)

                cACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaatctg                                                                                                                             <  1:948897/141‑1 (MQ=255)

                  cTGTCTGAATCTTAATCTCTATCTGAATTTTAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAg                                                                                                                           <  1:134138/141‑1 (MQ=14)

                  cTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTTTCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAg                                                                                                                           <  2:46225/141‑1 (MQ=255)

                  atcgcTGAATCCTAATAGCTATCTGAATCCGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCCGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAg                                                                                                                           <  2:1024146/137‑1 (MQ=14)

                     tCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGACTCGCTGTCTGAGTTTGAGTTATTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCg                                                                                                                        <  1:921536/141‑1 (MQ=14)

                     tCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTACGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCg                                                                                                                        <  1:800457/141‑1 (MQ=255)

                      cTGACTCCGAATCGCTCTCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGc                                                                                                                       <  1:824629/141‑1 (MQ=255)

                      cTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTTTCTGAGTCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTGTATCTCGCGATCTGAATCTGAGTCGc                                                                                                                       <  2:324552/141‑1 (MQ=11)

                      cTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGc                                                                                                                       >  1:992666/1‑141 (MQ=255)

                        gAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTa                                                                                                                     <  1:892784/141‑1 (MQ=255)

                           tCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATGTGAGTCGCTGTCTGAGTGTGAATCGCTATCTGAACGTGAGTCGCTAtct                                                                                                                  >  2:166563/1‑141 (MQ=255)

                            ccGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAGTCTGAGTCGCTGTCTGAGACTGAATCGCTATGTGAAGCTGAGGCGCTAtgtg                                                                                                                 >  2:860201/1‑141 (MQ=14)

                            ccGAACCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctg                                                                                                                 <  1:460021/141‑1 (MQ=255)

                             cGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctga                                                                                                                <  2:792267/141‑1 (MQ=255)

                                aTCGCTATCTGATTCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtc                                                                                                             <  1:834978/141‑1 (MQ=255)

                                   gCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctga                                                                                                          <  1:401541/141‑1 (MQ=255)

                                      ctctgaatctgaaTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtc                                                                                                       <  1:333382/140‑1 (MQ=255)

                                      atcttaatctgaATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtc                                                                                                       <  1:851400/141‑1 (MQ=255)

                                      atctgaatctgaATCGCTATCCGAGTCTGAGTCTCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtc                                                                                                       <  1:24166/141‑1 (MQ=255)

                                      atctgaatctgaATCGCTATCCGAGACTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtc                                                                                                       <  1:524042/141‑1 (MQ=255)

                                       tctgaatctgaaTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGGGTCGCTGtctgagtctgaggcg                                                                                                      >  1:391770/1‑141 (MQ=255)

                                       tctgaatctgaaTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcg                                                                                                      >  1:862413/1‑141 (MQ=255)

                                       tctgaatctgaaTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcg                                                                                                      >  1:875053/1‑141 (MQ=255)

                                        ctgcatctgagtCGCGATCTGAGTCTGTGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgc                                                                                                     <  2:373820/141‑1 (MQ=11)

                                        ctgaatctgaatCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgc                                                                                                     <  1:775728/141‑1 (MQ=255)

                                         tgagtctgagtcGCTATTATTTTCTTAGTCGCTCTCTGACTCTGAATCTCTCTCCGTATCCGAGTCGCTATCTGAATCTCAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgct                                                                                                    <  2:617192/141‑1 (MQ=2)

                                            atctgaatcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtc                                                                                                 >  1:296669/1‑141 (MQ=255)

                                            atctgaatcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtc                                                                                                 >  1:805433/1‑141 (MQ=255)

                                              ctgaatcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctg                                                                                               >  1:1025067/1‑141 (MQ=255)

                                                 aatcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctgagt                                                                                            <  1:17936/141‑1 (MQ=255)

                                                    cGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctgagtctg                                                                                         >  1:550598/1‑141 (MQ=255)

                                                    cGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcactgtctgagtctg                                                                                         >  1:543751/1‑141 (MQ=255)

                                                     gCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTAtctgagtccgagtcgctctctgaatctga                                                                                        >  2:659055/1‑141 (MQ=255)

                                                     gCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTCtctgagtgtgagtcgctgtctcagtctga                                                                                        >  2:661790/1‑141 (MQ=255)

                                                       tATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctgagtctgagt                                                                                      >  1:808680/1‑141 (MQ=255)

                                                        atcttattCTGCGTCGCTTTCTGAATCTGAATCGCTGTCTGAGTCCGAATCCCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctgagtctgagtc                                                                                     <  1:856365/134‑1 (MQ=14)

                                                        aTCCGAGTCTGAGTCGCTGTCTGTATTTGAATCTCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctgagtctgagtc                                                                                     <  2:870945/141‑1 (MQ=255)

                                                        aTCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctgagtctgagtc                                                                                     <  1:601315/141‑1 (MQ=255)

                                                        aTCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGGATCGCTGtctgagtccgagccgctgtctgagtccgcgtc                                                                                     >  2:73902/1‑141 (MQ=14)

                                                          ccGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAATCGCTATCTGATTGTGAGTCGCTCgctgagtctgagtccctatctgagtctgagtagc                                                                                   >  2:911567/1‑141 (MQ=14)

                                                            gAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTAtctgagtctgagtcgctgtctgaatctgcgacgcta                                                                                 >  2:543676/1‑140 (MQ=255)

                                                                cTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGagtctgagtcactgtctgagtctgagtcgctgtctg                                                                             >  1:580318/1‑141 (MQ=255)

                                                                  gtgtggCTGTCTGTGTCTGAGTCTCTGTTTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTATCTGAGtctgagtcgctgtctgagtctgagtcgctgtctgag                                                                           <  2:487538/136‑1 (MQ=11)

                                                                  gAGTGGCTGCCTGCATCTGAACCACTGTCTTAGTCCGAATCGTTATCTGAAACTGAGTCGCTGTCTGAGTCTGATTCGCGATCTGAATCTGAGTCGCTATCTGAGtctgagtcgctgtctgagtctgagtcgctgtctgag                                                                           <  1:597632/141‑1 (MQ=17)

                                                                  gAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTTTCTgaatctgaatcgctttctaggtctggttcgctgccttgg                                                                           >  2:696788/1‑138 (MQ=11)

                                                                  gAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGtctgagtcgctgtctgagtctgagtcgctgtctgag                                                                           <  1:411339/141‑1 (MQ=255)

                                                                  gAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGtctgagtcgctgtctgagtctgagtcgctgtctgag                                                                           <  1:326074/141‑1 (MQ=255)

                                                                  gAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGtctgagtcgctgtctgagtctgagtcgctgtctgag                                                                           <  1:798600/141‑1 (MQ=255)

                                                                  gAGTCGCTGTCTGAATCGGGATCGCTGTCTGAGTCTGAAGTGCTATTTGAATCTGAGTCGCTGTCTTAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGtctgagtcgctgtctgagtctgagtcgctgtctgag                                                                           <  2:892444/141‑1 (MQ=17)

                                                                   aGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAGTCTGAGGCGCTTTCTGAGTCTGAATCGCTGTCTGATTCTGAGTCGCTATCTGAGTctgagtcgctgtctgagtctgagtcgctgtctgagt                                                                          >  2:125951/1‑141 (MQ=14)

                                                                    gtggCTGTCTGAATCTGTGTCGCTGTCTGAGTCCGAATCCCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTATCTGAGTCtgagtcgctgtctgagtctgagtcgctgtctgagtc                                                                         <  2:57452/138‑1 (MQ=17)

                                                                    gTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCtgagtcgctgtctgagtctgagtcgctgtctgagtc                                                                         <  1:208464/141‑1 (MQ=255)

                                                                    gTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCtgagtcgctgtctgagtctgagtcgctgtctgagtc                                                                         >  1:232268/1‑141 (MQ=255)

                                                                        cTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGaa                                                                     >  1:471738/1‑141 (MQ=255)

                                                                               aatctgaatcGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CGCTat                                                             >  1:615786/1‑141 (MQ=255)

                                                                                   tgaatcGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGATTCTGAGT‑CGCTgtctga                                                         >  2:340535/1‑141 (MQ=11)

                                                                                       tcGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTGTCTGAGACTGAAT‑CAGTgtctgatact                                                     >  2:269942/1‑137 (MQ=11)

                                                                                        ctctgtctgtGTCTTAGTCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGATTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CGCTatctgaatctg                                                    <  2:118635/139‑1 (MQ=14)

                                                                                         gCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CGCTatctgaatctgg                                                   >  1:499952/1‑140 (MQ=255)

                                                                                         gCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CGCTatctgaatctga                                                   >  1:957954/1‑141 (MQ=255)

                                                                                             tCTGAGTCCGAATCGCTATCTGACTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CGCTATCTGAATCTGAGTCg                                               <  1:987498/141‑1 (MQ=255)

                                                                                             tCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGACTCGCTGTCTGAGTCTGAGTCGCTGTCTGAACCTGAGT‑CGCTatatgaatccgcctcc                                               >  2:45893/1‑134 (MQ=11)

                                                                                              cTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CGCTGTCTGAATCTGAGtggg                                              >  2:949631/1‑138 (MQ=14)

                                                                                                 aGTCCGAATCGCCATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGCGTCCGAGTCGCTCTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CGCTATCTGAATCTGAGTCGCTgt                                           <  1:147326/141‑1 (MQ=255)

                                                                                                  gTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CGCTATCTGAATCTGAGTCGCTgtc                                          <  1:309508/141‑1 (MQ=255)

                                                                                                  gTCCGAACCGCAATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CGCTATCTGAATCTGAGTCGCTgtc                                          <  1:734314/141‑1 (MQ=255)

                                                                                                    ccGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CTCTATCTGAGTCCGAGTCGCTgtctg                                        >  2:428889/1‑141 (MQ=255)

                                                                                                        aTCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CGCTATCTGAATCTGAGTCGCTgtctgagtc                                    >  1:1011601/1‑141 (MQ=255)

                                                                                                         tCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTGTGAAT‑CGCTCTCTGAATCTGCGTCGCTgtctgaatct                                   >  2:983926/1‑141 (MQ=255)

                                                                                                         tCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCGATCTGAGTCTGAGTGGCCTTCAAAAGCTTAGTCGCTGTCTGTGTCTGCCT‑CGCTATCTGCATCTGAGTCGCAgtctgagtct                                   >  1:240170/1‑141 (MQ=14)

                                                                                                          tgCTATCTGATTCTGAGTCGGTGTCTTAGTCCCAGTCCGTGTCTGAGTCTTAGTAACTAGCGTAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTGTGAG‑ATGCATGCGCTATCTGAATCTGAGTCGCTgtctgagtctg                                  <  2:417245/140‑1 (MQ=2)

                                                                                                                       tgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctga                     >  1:486477/1‑141 (MQ=255)

                                                                                                                       tgaGTCGCTGTCTGAGGCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGATTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctga                     <  2:219854/141‑1 (MQ=14)

                                                                                                                       tgaGTCGCTGGCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctga                     <  1:302739/141‑1 (MQ=255)

                                                                                                                       ggtgTCGCTGTCTTAGTCTGATTCGCTATCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGT‑CGCTATCTGAgtctgagtcgctgtctgagtctgagtcgctgtctga                     <  2:607562/138‑1 (MQ=14)

                                                                                                                          gTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CGCTATCTGAATCtgagtcgctgtctgagtctgagtcgctgtctgagtc                  >  1:382031/1‑141 (MQ=255)

                                                                                                                           tCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGACT‑CGCTGTCTGAATCTGACTCGCCGTCACAGtctgagtcgctctctgaatcc                 >  2:965513/1‑140 (MQ=14)

                                                                                                                           tCGCGGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatct                 <  2:932950/141‑1 (MQ=255)

                                                                                                                            ctcTGTCTTATTTTGAGTTGCTATTTGAATCTGTTTCGCTTTCTGAGTCTGCGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctg                <  2:969830/139‑1 (MQ=14)

                                                                                                                             tcTGTCTTAGTCTGATTCTCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTTAGTCGCTGTCTGAGTCTGAAT‑CGCTATCTGAAGCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctga               <  1:468047/140‑1 (MQ=17)

                                                                                                                             gCTGTCTGAGTCTGATTCGCTCTCTGAGTCTGTGTCGCTCTCTGAGTCTGAGTCGCTCTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctga               <  2:272368/141‑1 (MQ=14)

                                                                                                                             gCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGCCTGAAT‑CGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctga               <  1:375722/141‑1 (MQ=255)

                                                                                                                              cTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgag              >  1:228606/1‑141 (MQ=255)

                                                                                                                                 tctggtgctgaATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcg           <  1:772598/134‑1 (MQ=255)

                                                                                                                                    gagtctgaTTCGCTGTCTGAATCTGAGTCGCTATCTGTGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcgctg        <  2:559465/141‑1 (MQ=255)

                                                                                                                                          gaATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCAGAAG‑CGCTATCTGAGTCTGAGTCTCTGTCTGAGtctgagtcactatcagagtctgaatcgctgtctgag  >  2:10170/1‑140 (MQ=11)

CTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAAT‑CGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAA  >  USA300TCH1516_ALE/2774829‑2775107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`