BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	96,908	Δ1 bp	6.5%	intergenic (+1424/+55)	gloB → / ← USA300HOU_0095	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	96,908	0	T	.	6.5%	568.0 / 31.3	169	intergenic (+1424/+55)	gloB/USA300HOU_0095	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (87/70); new base . (5/6); total (92/77)
Fisher's exact test for biased strand distribution p-value = 5.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.43e-01

Only 100 of 171 total aligned reads displayed.

CAGTGTTGTGTTTATATGTCGTGAATTTTTAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTCCATTTCTTGCCAGATATTCA  >  CP000730/96770‑97048

cAGTGTTGTGTTTATATGTCGTGAATTTTTAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTaa                                                                                                                                             >  1:1161405/1‑141 (MQ=255)

  gtgttgtgttTATATGTCGTGAATTTTTAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAAtt                                                                                                                                           <  1:1438088/141‑1 (MQ=255)

    gttgtgttTATATGTCGTGAATTTTTAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑Ga                                                                                                                                        >  1:1110555/1‑141 (MQ=255)

     ttgtgttTATATGTCGTGAATTTTTAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑Gaa                                                                                                                                       >  2:914316/1‑141 (MQ=255)

       gtgttTATATGTCGTGAATTTTTAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑Gaaaa                                                                                                                                     <  2:1410559/141‑1 (MQ=255)

                tGTCGTGAATTTTTAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTGAGATGATTTTC‑AATTAGAAAatatcttac                                                                                                                             <  2:1398389/141‑8 (MQ=37)

                   cGTGAATTTTTAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTGAGATGATTTTC‑AATTAGAAAAT‑ATCTTActgc                                                                                                                         <  1:366329/141‑1 (MQ=37)

                   cGTGAATTTTTAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATAATCTCActgc                                                                                                                         >  1:484471/1‑141 (MQ=255)

                    gTGAATTTTTAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCActgct                                                                                                                        >  1:1066982/1‑141 (MQ=255)

                     tGAATTTTTAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCActgctg                                                                                                                       <  2:1041604/141‑1 (MQ=255)

                        aTTTTTAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGttt                                                                                                                    >  1:894247/1‑141 (MQ=255)

                          ttttAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGttttt                                                                                                                  <  1:1072193/141‑1 (MQ=255)

                           tttAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGtttttt                                                                                                                 >  2:142891/1‑141 (MQ=255)

                              aGCTCTAGATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTaaa                                                                                                              >  1:1313337/1‑141 (MQ=255)

                              aGCTCTAAATAGTATTAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTaaa                                                                                                              <  1:1378564/141‑1 (MQ=255)

                              aGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTaaa                                                                                                              <  1:272788/141‑1 (MQ=255)

                               gCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAg                                                                                                             >  1:1613878/1‑141 (MQ=255)

                               gCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAg                                                                                                             <  2:1613878/141‑1 (MQ=255)

                               gCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAg                                                                                                             <  2:922645/141‑1 (MQ=255)

                                 tctAAATAGTAAAAGATAGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTGAGATGATTTTC‑AATTAGAAAAT‑ATCTTACTGCTGtttctattttca                                                                                                           <  2:332234/141‑10 (MQ=18)

                                  ctAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGtt                                                                                                          >  2:1498653/1‑141 (MQ=255)

                                   tAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGttt                                                                                                         >  1:1320919/1‑141 (MQ=255)

                                    aaaTAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTta                                                                                                        <  1:119738/141‑1 (MQ=255)

                                    aaaTAGTATAAGATTGAAAAAGGTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAAGT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTta                                                                                                        <  1:1546516/141‑1 (MQ=255)

                                          tataAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCTTACAATTTGGATGATTATTAATATAGAAACTTCAGTAATAT‑TTTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTc                                                                                                  >  1:328073/1‑141 (MQ=37)

                                          tataAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTc                                                                                                  >  1:1335852/1‑141 (MQ=255)

                                          tataAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTc                                                                                                  <  1:234899/141‑1 (MQ=255)

                                          tataAGATTGAAAAAGTTGTTACTGCTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATTATCTCACTGCCGTTTTTTAAAGGTTTATACATc                                                                                                  >  1:1148286/1‑141 (MQ=255)

                                           ataAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCa                                                                                                 <  2:4543/141‑1 (MQ=255)

                                            taAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAATCAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCAt                                                                                                <  2:803487/141‑1 (MQ=255)

                                              aGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATcc                                                                                              >  2:1534053/1‑141 (MQ=255)

                                              aGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATcc                                                                                              >  1:902411/1‑141 (MQ=255)

                                                  tGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTa                                                                                          <  1:1646748/141‑1 (MQ=255)

                                                    aaaaaGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTaaa                                                                                        >  2:229054/1‑141 (MQ=255)

                                                     aaaaGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAat                                                                                       >  1:1009765/1‑141 (MQ=255)

                                                          ttgttACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTAttt                                                                                  <  1:844085/141‑1 (MQ=255)

                                                           tgttACTGTTTTAAATNATCACGAAGAAGNCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGCTTTCTAAAGGTTTATACCTCATCCTCTAAATTATTTa                                                                                 >  1:1216855/1‑141 (MQ=255)

                                                           tgttACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTa                                                                                 <  1:74073/141‑1 (MQ=255)

                                                            gttACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTaa                                                                                >  1:476679/1‑141 (MQ=255)

                                                             ttACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTaaa                                                                               <  1:1138351/141‑1 (MQ=255)

                                                             ttACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTaaa                                                                               >  2:330675/1‑141 (MQ=255)

                                                              tACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTaaaa                                                                              <  1:1422583/141‑1 (MQ=255)

                                                                   ttttAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAaataat                                                                         <  1:1463608/141‑1 (MQ=255)

                                                                    tttAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAAtaatt                                                                        <  2:1520023/141‑1 (MQ=255)

                                                                        aaTGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATg                                                                    >  1:1130384/1‑141 (MQ=255)

                                                                        aaTGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATg                                                                    >  1:698981/1‑141 (MQ=255)

                                                                         aTGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTCTACCTCATCCTCTACATTACTTAAAAATACTTAATgg                                                                   >  2:781421/1‑141 (MQ=255)

                                                                          tGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGt                                                                  >  2:966962/1‑141 (MQ=255)

                                                                           gATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTa                                                                 <  1:554097/141‑1 (MQ=255)

                                                                              cACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTAttt                                                              >  2:1064594/1‑141 (MQ=255)

                                                                                cGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGa                                                            >  1:468674/1‑141 (MQ=255)

                                                                                cGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGa                                                            <  1:1521319/141‑1 (MQ=255)

                                                                                 gATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAg                                                           >  1:314155/1‑141 (MQ=255)

                                                                                 gATGAAGTCATTCAAGAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAg                                                           <  1:1373962/141‑1 (MQ=255)

                                                                                    gAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCAc                                                        >  2:912739/1‑141 (MQ=255)

                                                                                         cATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTa                                                   <  2:1335852/141‑1 (MQ=255)

                                                                                            tCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCg                                                <  2:512155/141‑1 (MQ=255)

                                                                                              aaTAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGAc                                              <  1:401715/141‑1 (MQ=255)

                                                                                              aaTAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGAc                                              <  1:1351985/141‑1 (MQ=255)

                                                                                              aaTAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGAc                                              <  1:614278/141‑1 (MQ=255)

                                                                                               aTAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACt                                             >  2:680293/1‑141 (MQ=255)

                                                                                                tAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACtt                                            <  2:610298/141‑1 (MQ=255)

                                                                                                 aaGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTTTTTTTTTTTACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACttt                                           >  2:569091/1‑141 (MQ=38)

                                                                                                   gAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTAt                                         >  1:441985/1‑141 (MQ=255)

                                                                                                   gAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTAt                                         >  1:121811/1‑141 (MQ=255)

                                                                                                     aTGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTAtga                                       >  2:824623/1‑141 (MQ=255)

                                                                                                        aTTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAACATAATTAATGGTATTTGAGCACGTTTAGCGACTTTAtgactg                                    >  2:1583433/1‑141 (MQ=255)

                                                                                                          tATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTAtgactgac                                  <  2:1371360/141‑1 (MQ=255)

                                                                                                            tGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGTTATTTGAGCACGTTTAGCGACTTTATGACTGACAt                                <  2:1217605/141‑1 (MQ=255)

                                                                                                            tGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACAt                                >  1:1631611/1‑141 (MQ=255)

                                                                                                            tGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACAt                                >  2:272498/1‑141 (MQ=255)

                                                                                                            tGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACAt                                <  2:684140/141‑1 (MQ=255)

                                                                                                            tGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACAt                                >  2:1297080/1‑141 (MQ=255)

                                                                                                            tGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACAt                                >  2:147178/1‑141 (MQ=255)

                                                                                                              aaaaTAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTa                              <  1:541558/141‑1 (MQ=255)

                                                                                                                  tAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTGCCTGCGGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCaa                          >  1:380362/1‑141 (MQ=255)

                                                                                                                  tAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCaa                          <  1:387838/141‑1 (MQ=255)

                                                                                                                  tAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCaa                          >  2:375424/1‑141 (MQ=255)

                                                                                                                     aaaCAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAAttt                       <  2:235583/141‑1 (MQ=255)

                                                                                                                     aaaCAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAAttt                       >  1:1174946/1‑141 (MQ=255)

                                                                                                                     aaaCAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAAttt                       >  1:638498/1‑141 (MQ=255)

                                                                                                                      aaCAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTc                      >  2:790911/1‑141 (MQ=255)

                                                                                                                      aaCAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTc                      >  2:1480104/1‑141 (MQ=255)

                                                                                                                      aaCAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTc                      >  2:595716/1‑141 (MQ=255)

                                                                                                                       aCAGCAGTGAGATGATTTTC‑AATTAGAAAAT‑ATCTTACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTcc                     >  1:771958/1‑141 (MQ=37)

                                                                                                                       aCAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTAGTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTcc                     <  2:428889/141‑1 (MQ=255)

                                                                                                                         agtagTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTCCAt                   <  2:1392613/138‑1 (MQ=255)

                                                                                                                         agcagTGAGATGATTTTC‑AATTAGAAAAT‑ATCTTACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTCCAt                   >  1:58610/1‑141 (MQ=37)

                                                                                                                         agcagTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTCCAt                   <  2:638498/141‑1 (MQ=255)

                                                                                                                         agcagTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTCCAt                   <  2:1521136/141‑1 (MQ=255)

                                                                                                                            agTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTCCCAATTTCCATTTc                >  2:18170/1‑141 (MQ=255)

                                                                                                                                aGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAATGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTCCATTTCTTGc            >  2:1113602/1‑141 (MQ=255)

                                                                                                                                aGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTCCATTTCTTGc            >  2:1305621/1‑141 (MQ=255)

                                                                                                                                 gAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTCCATTTCTTGcc           <  2:1133017/141‑1 (MQ=255)

                                                                                                                                   t‑atTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTCCATTTCTTGCCAg         <  1:1482579/141‑1 (MQ=255)

                                                                                                                                        ttCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTCCATTTCTTGCCAGatat     <  2:875277/141‑1 (MQ=255)

                                                                                                                                        ttCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTCCATTTCTTGCCAGatat     >  1:749768/1‑141 (MQ=255)

                                                                                                                                          cTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTCCATTTCTTGCCAGATATTc   >  2:157848/1‑141 (MQ=255)

                                                                                                                                           tAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTCCATTTCTTGCCAGATATTCa  <  1:1297080/141‑1 (MQ=255)

                                                                                                                                           tAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTCCATTTCTTGCCAGATATTCa  <  1:147178/141‑1 (MQ=255)

CAGTGTTGTGTTTATATGTCGTGAATTTTTAGCTCTAAATAGTATAAGATTGAAAAAGTTGTTACTGTTTTAAATGATCACGATGAAGTCATTCAATAAGAATGATTATGAAAATAGAAACAGCAGTAAGAT‑ATTTTCTAATT‑GAAAATCATCTCACTGCTGTTTTTTAAAGGTTTATACCTCATCCTCTAAATTATTTAAAAATAATTAATGGTATTTGAGCACGTTTAGCGACTTTATGACTGACATTACCAATTTCCATTTCTTGCCAGATATTCA  >  CP000730/96770‑97048

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`