Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	111,512	A→G	34.0%	S194S (TCA→TCG)	USA300HOU_0110 →	possible staphylococcal tandem lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	111,512	0	A	G	34.0%	101.8 / 84.4	91	S194S (TCA→TCG)	USA300HOU_0110	possible staphylococcal tandem lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (32/28);  new base G (12/19);  total (44/47)
Fisher's exact test for biased strand distribution p-value = 2.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

ACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAA  >  CP000730/111375‑111650                                                                                                                                          |                                                                                                                                            accgAGCAAGTTAAAGAAGAAAACATACAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCt                                                                                                                                          <  2:190380/138‑1 (MQ=255)     aGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAat                                                                                                                                      <  1:549940/141‑1 (MQ=255)     aGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAat                                                                                                                                      <  1:648990/141‑1 (MQ=255)      ggAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAata                                                                                                                                     >  1:679472/1‑141 (MQ=255)        aaGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATc                                                                                                                                   >  1:624459/1‑141 (MQ=255)            tAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAAtt                                                                                                                               <  1:327539/141‑1 (MQ=255)             aaaGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTa                                                                                                                              >  2:35040/1‑141 (MQ=255)              aagaagAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTaa                                                                                                                             <  1:399088/141‑1 (MQ=255)              aagaagAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTaa                                                                                                                             >  1:9522/1‑141 (MQ=255)                gaagaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACt                                                                                                                           <  1:220190/141‑1 (MQ=255)                  agaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTTCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTaa                                                                                                                         >  2:199344/1‑141 (MQ=255)                  agaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTaa                                                                                                                         <  2:661506/141‑1 (MQ=255)                   gaaAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAat                                                                                                                        >  2:358080/1‑141 (MQ=255)                    aaaaCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAatg                                                                                                                       <  2:240721/141‑1 (MQ=255)                    aaaaCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAatg                                                                                                                       <  1:85562/141‑1 (MQ=255)                     aaaCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAatga                                                                                                                      <  1:478230/141‑1 (MQ=255)                      aaCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAatgat                                                                                                                     >  1:83673/1‑141 (MQ=255)                      aaCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAatgat                                                                                                                     <  1:560453/141‑1 (MQ=255)                      aaCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAatgat                                                                                                                     <  1:298643/141‑1 (MQ=255)                      aaCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAatgat                                                                                                                     >  2:667572/1‑141 (MQ=255)                       aCATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAatgatg                                                                                                                    >  1:80596/1‑141 (MQ=255)                        cATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAAtgatga                                                                                                                   <  2:176883/141‑1 (MQ=255)                          tAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGTGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGAtt                                                                                                                 >  2:400399/1‑141 (MQ=255)                           aaaaaaaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATta                                                                                                                <  1:487595/141‑1 (MQ=255)                            aaaaaaGAAATCGAAAACTTTAAGTTTTTTGCGTAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATAACAATTAACTAATGATGATtat                                                                                                               >  2:518099/1‑141 (MQ=255)                            aaaaaaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATtat                                                                                                               <  1:209729/141‑1 (MQ=255)                            aaaaaaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATtat                                                                                                               >  1:467530/1‑141 (MQ=255)                            aaaaaaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATtat                                                                                                               <  2:548208/141‑1 (MQ=255)                             aaaaaGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTCAATATCAATTAACTAATGATGATtata                                                                                                              >  2:337342/1‑141 (MQ=255)                                 aGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGt                                                                                                          >  2:208877/1‑141 (MQ=255)                                 aGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGt                                                                                                          <  2:60301/141‑1 (MQ=255)                                   aaaTCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑aa                                                                                                       <  2:440747/141‑1 (MQ=255)                                   aaaTCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑aa                                                                                                       <  1:223959/141‑1 (MQ=255)                                   aaaTCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑aa                                                                                                       <  1:214658/141‑1 (MQ=255)                                    aaTCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGT‑aaa                                                                                                      >  1:450649/1‑141 (MQ=255)                                       cGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGT‑AAAACa                                                                                                   >  1:560540/1‑141 (MQ=255)                                            aCTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTAc                                                                                              <  2:74266/141‑1 (MQ=255)                                            aCTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTAc                                                                                              >  1:475455/1‑141 (MQ=255)                                              tttAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACgc                                                                                            <  1:550790/141‑2 (MQ=255)                                                  aGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGCaaaa                                                                                        <  1:342344/141‑1 (MQ=255)                                                  aGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCAGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTaaaa                                                                                        >  2:461140/1‑141 (MQ=255)                                                      ttttGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGCAAAAGata                                                                                    <  1:667751/141‑1 (MQ=255)                                                         tGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGa                                                                                 >  1:595766/1‑141 (MQ=255)                                                         tGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGa                                                                                 <  2:613170/141‑1 (MQ=255)                                                         tGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGa                                                                                 >  1:92312/1‑141 (MQ=255)                                                            gcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGCAAAAgatatgatat                                                                              >  2:164281/1‑141 (MQ=255)                                                            gcAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGCAAAAGATATGatat                                                                              <  2:544542/141‑4 (MQ=14)                                                             ttttttttcAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGTCTATATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGCAAAAGATATGAtata                                                                             >  2:35358/9‑141 (MQ=11)                                                             cAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATa                                                                             <  2:411197/141‑1 (MQ=255)                                                                 ataGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGCAAAAGATATGATATAccga                                                                         <  1:508147/141‑3 (MQ=14)                                                                   aGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATGATATAATCCAGAGGTGCAGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGCAAAAGATATGATATAccgacg                                                                       <  2:453738/141‑5 (MQ=11)                                                                    gCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACcaacaa                                                                      >  1:699214/1‑141 (MQ=255)                                                                     cAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACgc                                                                                            <  2:44390/118‑2 (MQ=255)                                                                     cAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACgc                                                                                            >  1:44390/1‑117 (MQ=255)                                                                        ttttAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATaa                                                                  >  2:85998/1‑141 (MQ=255)                                                                         tttAAAAATTTAAAGAATTATAAAGATGGAGATATATCATATAATCGAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATTATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATaaa                                                                 <  2:457341/141‑1 (MQ=255)                                                                           tAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAgc                                                               <  1:220657/141‑1 (MQ=255)                                                                             aaGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAgcgc                                                             >  1:215956/1‑141 (MQ=255)                                                                              aGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGcc                                                            <  1:440127/141‑1 (MQ=255)                                                                                aTTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGATTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGa                                                          <  2:414608/141‑1 (MQ=255)                                                                                  ttAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAg                                                        <  2:527341/141‑1 (MQ=255)                                                                                     aTGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCtg                                                     <  1:337316/141‑1 (MQ=255)                                                                                      tGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCtgt                                                    <  1:263745/141‑1 (MQ=255)                                                                                         aTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCtgttgt                                                 >  1:177298/1‑141 (MQ=255)                                                                                            ataAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGa                                              >  1:464596/1‑141 (MQ=255)                                                                                            ataAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGa                                              <  2:358477/141‑1 (MQ=255)                                                                                             taAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGTAAAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGa                                              >  1:458472/1‑141 (MQ=255)                                                                                                aGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAgg                                          <  2:320763/141‑1 (MQ=255)                                                                                                   tGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTAc                                       >  1:630916/1‑141 (MQ=255)                                                                                                      agaTATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAgg                                    >  1:294124/1‑141 (MQ=255)                                                                                                       gaTATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGa                                                                                      >  2:653197/1‑90 (MQ=255)                                                                                                       gaTATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGa                                                                                      <  1:653197/90‑1 (MQ=255)                                                                                                       gaTATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAggg                                   <  2:402328/141‑1 (MQ=255)                                                                                                                   aaTCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGt                       <  1:506804/141‑1 (MQ=255)                                                                                                                      ccAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtca                    >  2:37798/1‑141 (MQ=255)                                                                                                                      ccAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGACGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAATGTACAGGGAATTTAAAAGGTtca                    >  2:92468/1‑141 (MQ=255)                                                                                                                        agagGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGcc                                                            >  1:148937/1‑99 (MQ=255)                                                                                                                        agagGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGcc                                                            <  2:148937/99‑1 (MQ=255)                                                                                                                        agagGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTtcatc                  >  1:567262/1‑141 (MQ=255)                                                                                                                           ggTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTAAGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGt               <  2:245191/141‑1 (MQ=255)                                                                                                                              gCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTgg            >  1:296712/1‑141 (MQ=255)                                                                                                                              gCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTgg            >  1:157139/1‑141 (MQ=255)                                                                                                                              gCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAAATTAAAAGGTTCATCAGTTgg            >  2:440830/1‑141 (MQ=255)                                                                                                                                cGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGat          >  2:74488/1‑141 (MQ=255)                                                                                                                                 gAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGata         <  2:616663/141‑1 (MQ=255)                                                                                                                                 gAGTTATTCAGCTCAATATCAATTAACTAATGAGGATTATAATGT‑AAAACAATTACGTAAAATATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGata         >  2:417917/1‑141 (MQ=255)                                                                                                                                   gTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGAtata       >  1:469563/1‑141 (MQ=255)                                                                                                                                     tATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAAt                                                                                                 <  1:535228/49‑1 (MQ=255)                                                                                                                                     tATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAAt                                                                                                 >  2:535228/1‑49 (MQ=255)                                                                                                                                     tATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATaaa     <  1:21109/141‑1 (MQ=255)                                                                                                                                        tCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATaaaaaa  >  2:620957/1‑141 (MQ=255)                                                                                                                                          |                                                                                                                                            ACTGAGGAAGTTAAAGAAGAAAACATAAAAAAAGAAATCGAAAACTTTAAGTTTTTTGCGCAATATAGCAATTTTAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCAGCTCAATATCAATTAACTAATGATGATTATAATGT‑AAAACAATTACGTAAAAGATATGACATACCAACAAATAAAGCGCCGAAGCTGTTGTTGAAAGGTACAGGGAATTTAAAAGGTTCATCAGTTGGATATAAAAAA  >  CP000730/111375‑111650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑