Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 613,998 G→A 6.4% S770S (TCG→TCA)  sdrC → Ser‑Asp rich fibrinogen/bone sialoprotein‑binding protein SdrC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730613,9980GA 6.4% 305.1 / 3.9 109S770S (TCG→TCA) sdrCSer‑Asp rich fibrinogen/bone sialoprotein‑binding protein SdrC
Reads supporting (aligned to +/- strand):  ref base G (49/52);  new base A (3/5);  total (52/57)
Fisher's exact test for biased strand distribution p-value = 7.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 112 total aligned reads displayed.

TCTGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGA‑TAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGA  >  CP000730/613858‑614138
                                                                                                                                            |                                                                                                                                             
tCTGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCg                                                                                                                                               <  2:763519/141‑1 (MQ=255)
 cTGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCgg                                                                                                                                              <  1:155861/141‑1 (MQ=255)
   gACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGAt                                                                                                                                            >  1:69052/1‑141 (MQ=255)
     gtctgaatcgctgtcagatagcgactcagattcagatagcgactcagatTCAGACAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAg                                                                                                                                          >  1:57802/14‑141 (MQ=255)
     cAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAg                                                                                                                                          <  1:622813/141‑1 (MQ=255)
     cAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAg                                                                                                                                          <  1:949997/141‑1 (MQ=255)
        cGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGGCAGCGATTCAGACTCGGATAGCga                                                                                                                                       >  1:765087/1‑141 (MQ=255)
        cGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCga                                                                                                                                       <  1:840615/141‑1 (MQ=255)
         gATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCgac                                                                                                                                      <  1:1011077/141‑1 (MQ=255)
         gATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCgac                                                                                                                                      <  2:171126/141‑1 (MQ=255)
         gATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGAATCAGATTCAGACAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCgac                                                                                                                                      >  1:573728/1‑141 (MQ=255)
          aTTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCgact                                                                                                                                     <  2:40236/141‑1 (MQ=255)
             cAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCgactcag                                                                                                                                  <  1:762451/141‑1 (MQ=255)
              agacagcgattcagattcagatagcgattcagattccgacagtgactcaGATTCCGACAGTGACTCGGATTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCAGAGAGCgactcaga                                                                                                                                 <  2:629364‑M1/92‑1 (MQ=255)
              aGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGGCTCAGACTCAGACAGCGATTCAGACTCGGATAGCgactcaga                                                                                                                                 <  2:285226/141‑1 (MQ=255)
              aGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCgactcaga                                                                                                                                 <  2:185361/141‑1 (MQ=255)
               gACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCgactcagac                                                                                                                                >  1:508039/1‑141 (MQ=255)
               gACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACAGCGACACCGACTCAGACAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCGGACAGCgactcagac                                                                                                                                >  2:579353/1‑141 (MQ=18)
                 cTCAGATAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCgactcagactc                                                                                                                              <  2:1078860/141‑1 (MQ=255)
                    agatagcgactcagattcagatagcgactcagattcAGACAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGActcagactcaga                                                                                                                           >  2:199903/1‑141 (MQ=255)
                    agatagcgactcagattcagatagcgactcagattcAGACAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGActcagactcaga                                                                                                                           <  2:65528/141‑1 (MQ=255)
                       tagcgactcagattcagatagcgactcagattcagaCAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGA‑TAg                                                                                                                       <  1:955603/141‑1 (MQ=255)
                         gcgactcagattcagatagcgactcagattcagaCAGCGATTCAGACAGAGACTGAGACTCAGATAGCGATTCAGATTCAGACGGCGACTCAGACTCGGATGGCGATTGAGAAGCGGATAGCGACACAGACTCAGA‑TAGCg                                                                                                                     >  2:944327/1‑141 (MQ=18)
                          cgcctcagattcagatagcgactcagattcagaCAGCGATTCAGACAGCGACACAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGA‑TAGCGa                                                                                                                    <  1:225967/138‑1 (MQ=255)
                          cgactcagattcagatagcgactcagattcagaCAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGA‑TAGCGa                                                                                                                    <  2:957767/141‑1 (MQ=255)
                          cgactcagattcagatagcgactcagattcagaCAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGA‑TAGCGa                                                                                                                    <  2:124869/141‑1 (MQ=255)
                          cgactcagattcagatagcgactcagattcagaCAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGA‑TAGCGa                                                                                                                    <  1:147422/141‑1 (MQ=255)
                          cgactcagagtcagatagcgactcagattcagaCAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGA‑TAGCGa                                                                                                                    <  1:819685/141‑1 (MQ=255)
                           gactcagattcagatagcgactcagattcagaCAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGAGAGCGATTGAGACTCAGATAGCGACTCAGACTCAGA‑TAGCGAc                                                                                                                   >  2:1086395/1‑141 (MQ=255)
                           gactcagattcagatagcgactcagattcagaCAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGA‑TAGCGAc                                                                                                                   >  1:274858/1‑141 (MQ=255)
                           gactcagattcagatagcgactcagattcagaCAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGA‑TAGCGAc                                                                                                                   <  2:459196/141‑1 (MQ=255)
                             ctcagattcagatagcgactcagattcagaCAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGA‑TAGCGACTc                                                                                                                 >  1:395075/1‑141 (MQ=255)
                               cagattcagatagcgcctcagattcagaCAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGA‑TAGCGACTCAg                                                                                                               <  1:59492/141‑1 (MQ=255)
                               cagattcagatagcgactcagattcagaCAGCGATTCAGACTCAGACTCAGACTCAGATACCGATCCAGATTCAGACACCGACCCAGACTCAGACAGAGACTCAGACTCGGAGCCGGACTCCGACGCAGC‑TAGCGatcccg                                                                                                               >  2:204587/1‑136 (MQ=17)
                               cagattcagatagcgactcagattcagaCAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGA‑TAGCGACTCAg                                                                                                               <  1:793382/141‑1 (MQ=255)
                                 gattcagatagcgactcagattcagaCAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGA‑TAGCGACTCAGAt                                                                                                             <  2:520918/141‑1 (MQ=255)
                                 gacagcgattcagactcagatagcgactcaGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGATTCAGACAGCGATTCAGATTCAGATAGCGATTCAGATTCAGA‑CAGCGACTCAGAt                                                                                                             <  1:1012559‑M1/111‑1 (MQ=255)
                                   ttcagatagcgactcagattcagaCAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGA‑TAGCGACTCATATTc                                                                                                           >  1:1042431/1‑141 (MQ=255)
                                    tcagatagcgactcagattcagaCAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGA‑TAGCGACTCAGATTCg                                                                                                          <  1:735211/141‑1 (MQ=255)
                                      tgattcagactcagacagcgactcaGATTCAGACAGTGATTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGA‑TAGCGACTCAGATTCGGa                                                                                                        >  1:327354‑M1/26‑141 (MQ=255)
                                       gattcagactcagacagcgactcaGATTCAGACAGTGATTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGA‑TAGCGACTCAGATTCGgac                                                                                                       <  1:923375‑M1/117‑2 (MQ=255)
                                       gatagcgactcagattcagaCAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGA‑TAGCGACTCAGATTCGGAt                                                                                                       <  2:516578/141‑1 (MQ=255)
                                             ttctcatattgagaAATCGATTATAACAGAGATGCAGACTCGGATAGCGATTCAGATTCAGACATCAACTCAGACTCAGACAGCGATTCAGCCTCGGATAGCGACTCAGAGTCAGA‑TAGCGACTCAGATTCGGATAGCgac                                                                                                 <  2:322728/139‑1 (MQ=14)
                                             gactcagattcagaCAGCGATTCAGACAGCGACTCAGACTCAGATGGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGA‑TAGCGACTCAGATTCGGATAGCgac                                                                                                 <  2:342025/141‑1 (MQ=255)
                                             gactcagattcagaCAGCGATTCAGACAGCGACACAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGAGAGCGATTCGGACTCGGATAGCGACTCAGACTCAGA‑TAGCGACACAGACTCAGATAGCgac                                                                                                 >  2:181920/1‑141 (MQ=18)
                                              actcagattcagaCAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGA‑TAGCGACTCAGATTCGGATAGCgact                                                                                                <  2:729861/141‑1 (MQ=255)
                                                tcagattcagaCAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTGAGACTCGGAGAGCGACTCAGACTCAGA‑GAGCGACTCAGACTCAGATAGCgactca                                                                                              >  2:778332/1‑141 (MQ=17)
                                                     gtcagacagggattcagacagcgaCTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGA‑TAGCGACTCAGATTCGGATAGCgactcagactc                                                                                         <  2:1060882/140‑1 (MQ=255)
                                                      acagacagcgattcagacagcgaCTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGA‑TAGCGACTCAGATTCGGATAGCgactcagactca                                                                                        <  2:1086571/140‑1 (MQ=255)
                                                       cagacagcgattcagacagcgaCTCAGACTCAGACAGAGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGA‑TAGCGACTCAGATTCGGATAGCGactcagactcag                                                                                       <  2:187298/141‑1 (MQ=255)
                                                        agatgacgatgcagacagcgaCTGAGGCTCAGATAGCGATGTAGATTCAGACAGCGACTCTGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGA‑TAGCGACTCAGATTCGGATAGCGActcagactcaga                                                                                      <  2:473768/135‑1 (MQ=17)
                                                         gacagcgattcagacagcgaCTCAGACTCAGATAGCGATTCAGACTCAGAGAGCGACTCAGATTCAGAAAGCGATTCAGAATCGGATAGCGAGTCGGAGTCAGAGTAG‑GTTTCAGAATCGGGTAGCgatgcgaacgcggag                                                                                     >  2:611740/1‑128 (MQ=2)
                                                             gcgattcagacagcgaCTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGA‑TAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCg                                                                                 <  2:62105/141‑1 (MQ=255)
                                                             gcgattcagacagcgaCTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGA‑TAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCg                                                                                 >  2:737040/1‑141 (MQ=255)
                                                              cgattcagacagcgaCTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGA‑TAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCga                                                                                <  2:1016529/141‑1 (MQ=255)
                                                               gaatcagatagcgaCTCAGAATCAGATAGCGAGTCAGATTCAGACAGAGACTCAGACTCAGACAGTGATTCAGACTCAGATAGCGATTCAGACTCAGA‑TAGCGATTCAGATTCGGATAGCGACTCAGACTCAGATAGCgat                                                                               <  2:492649/138‑1 (MQ=11)
                                                                   cagacagcgaCTCAGACNCAGATAGCGANTCAGNTTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGA‑TAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCgattcag                                                                           >  1:633542/1‑141 (MQ=255)
                                                                    agacagcgaCTCAGACTCAGATAGCGATTCAGAGTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACCCAGA‑TAGAGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCgattcaga                                                                          <  2:809005/141‑1 (MQ=255)
                                                                     gacagcgaCTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCGGATAGCGACTCAGACTCAGA‑TAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCgattgagac                                                                         >  2:884320/1‑140 (MQ=255)
                                                                       cagcgaCTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGA‑TAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCgattcagattc                                                                       >  1:459618/1‑141 (MQ=255)
                                                                          cgaCTCAGATTCAGATAGCGATTCAGATTCTGACAGCGTTTCAGACTCAGACAGCGATTCAGACTCGGATAGCGATTCAGATTCAGA‑TAGCGACTCATATTTGGATAGCGATGCAGATTCAGATTAGGAttcataatcaga                                                                    >  2:549146/1‑141 (MQ=14)
                                                                          cgaCTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGA‑TAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGAttcagattcaga                                                                    <  2:596798/141‑1 (MQ=255)
                                                                             ctcagactcagaTAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGA‑TAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAg                                                                 >  1:106052/1‑141 (MQ=255)
                                                                                agactcagaTAGCGATTCAGAATCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGA‑TAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGa                                                              <  2:1043116/141‑1 (MQ=255)
                                                                                    tcagaTAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATATCGACTCAGACTCAGA‑TAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCg                                                          >  1:703019/1‑141 (MQ=255)
                                                                                    tcagaTAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGA‑TAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGAGAACGACTcc                                                          >  2:410496/1‑140 (MQ=255)
                                                                                    tcagaTAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGA‑TAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCAGATAGCGAtaca                                                          >  2:13486/1‑138 (MQ=255)
                                                                                       gaTAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGA‑TAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGAc                                                       >  1:10578/1‑141 (MQ=255)
                                                                                       gaTAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGA‑TAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGAc                                                       >  1:105116/1‑141 (MQ=255)
                                                                                           gCGATTCAGATGCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGA‑TAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAg                                                   <  2:940704/141‑1 (MQ=255)
                                                                                               ttcagattcagaCAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGA‑TAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAg                                               <  1:773007/141‑1 (MQ=255)
                                                                                                 cagattcagaCAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGA‑TAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTg                                             <  1:230684/141‑1 (MQ=255)
                                                                                                  agattcagaCAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGA‑TAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTga                                            <  2:1068299/141‑1 (MQ=255)
                                                                                                     ttcagaCACCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGA‑TAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTgattc                                         <  2:69052/141‑1 (MQ=255)
                                                                                                      tcagaCAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGA‑TAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTgattca                                        >  1:245543/1‑141 (MQ=255)
                                                                                                       cagaCAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGA‑TAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTgattcag                                       >  1:206548/1‑141 (MQ=255)
                                                                                                       cagaCAGCGACTCAGACTCAGACAGCGATTCAGACTCGGACAGCGACTCAGACTCAGA‑TAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGAgattcag                                       >  2:934838/1‑141 (MQ=255)
                                                                                                         gaCAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGA‑TAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTgattcagat                                     >  1:21833/1‑141 (MQ=255)
                                                                                                             gCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGA‑TAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGattcagnttcag                                 >  1:305175/1‑141 (MQ=255)
                                                                                                              cGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGA‑TAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGAttcagattcaga                                <  1:594627/141‑1 (MQ=255)
                                                                                                                 ctcagactcagaCAGCGATTCAGACTCGGATAGCGACTCAGACTCAGA‑TAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTc                             >  1:357593/1‑141 (MQ=255)
                                                                                                                  tcagactcagacAGCGATTCAGACTCGGATAGCGACTCAGACTCAGA‑TAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCa                            >  1:524774/1‑141 (MQ=255)
                                                                                                                    agactcagacAGCGATTCAGACTCGGATAGCGACTCAGACTCAGA‑TAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGa                          <  1:533264/141‑1 (MQ=255)
                                                                                                                    agactcagacAGCGATTCAGACTCGGATAGCGACTCAGACTCAGA‑TAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTCATTCAGCTTCAGACTCAGa                          <  2:222884/141‑1 (MQ=255)
                                                                                                                    agactcagacAGCGATCCAGGGTCGGATAGCGACTCAGACTCAGA‑TAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGa                          <  1:826237/141‑1 (MQ=255)
                                                                                                                     gactcagacAGCGATTCAGACTCGGATAGCGACTCAGACTCAGA‑TAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGAt                         <  1:199903/141‑1 (MQ=255)
                                                                                                                     gactcagacAGCGATTCAGACTCGGATAGCGACTCAGACTCAGA‑TAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGATTCAGACTCAGAt                         >  2:937328/1‑141 (MQ=255)
                                                                                                                     gactcagacAGCGATTCAGACTCGGATAGCGACTCAGACTCAGA‑TAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGATTCAGACACAGAt                         >  2:685980/1‑141 (MQ=255)
                                                                                                                          agacAGCGATTCAGACTCGGATAGCGACTCAGACTCAGA‑TAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGa                    <  1:743802/141‑1 (MQ=255)
                                                                                                                          agacAGCGATTCAGACTCGGATAGCGACTCAGACTCAGA‑TAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGAGATTCAGATTCAGAcagagaatgcga                    >  2:768007/1‑130 (MQ=255)
                                                                                                                           gacAGCGATTCAGACTCGGATAGCGACTCAGACTCAGA‑TAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGAc                   <  1:405584/141‑1 (MQ=255)
                                                                                                                           gacAGCGATTCAGACTCGGATAGCGACTCAGACTCAGA‑TAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCAGACTCAGACAGCGATTCAGATTCAGACACAGATACCGAc                   >  2:96932/1‑141 (MQ=255)
                                                                                                                           gacAGCGATTCAGACTCAGATAGCGACTCAGACTCAGA‑TAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGAc                   <  2:592486/141‑1 (MQ=255)
                                                                                                                               gCGATTCAGACTCGGATAGCGACTCAGACTCAGA‑TAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAg               <  1:862036/141‑1 (MQ=255)
                                                                                                                                cGATTCAGACTCGGATAGCGACTCAGACTCAGA‑TAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGa              <  2:995693/141‑1 (MQ=255)
                                                                                                                                     cAGACTCGGATAGCGACTCAGACTCAGA‑TAGCGACTCAGATTCGGATAGCga                                                                                                  >  1:367888/1‑52 (MQ=255)
                                                                                                                                       gACTCGGATAGCGACTCAGACTCAGA‑TAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGAc       >  1:65276/1‑141 (MQ=255)
                                                                                                                                         cTCGGATAGCGACTCAGACTCAGA‑TAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAg     <  1:927234/141‑1 (MQ=255)
                                                                                                                                         cTCGGATAGCGACTCAGACTCAGA‑TAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAg     >  1:640141/1‑141 (MQ=255)
                                                                                                                                            ggATAGCGACTCAGACTCAGA‑TAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGa  <  1:189155/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                             
TCTGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGA‑TAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGA  >  CP000730/613858‑614138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑