BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	864,720	G→A	5.8%	E12E (GAG→GAA)	est →	carboxylesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	864,720	0	G	A	5.8%	281.5 / 4.5	102	E12E (GAG→GAA)	est	carboxylesterase
Reads supporting (aligned to +/- strand): ref base G (39/52); new base A (2/4); total (46/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.62e-01

Only 100 of 105 total aligned reads displayed.

GTAAATGTCGGGCTTTTTTATTTATAATTAAGAATGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGA  >  CP000730/864581‑864860

gTAAATGTCGGGCTTTTTTATTTATAATTAAGAATGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAgg                                                                                                                                              <  2:341423/141‑1 (MQ=255)

 tAAATGTCGGGCTTTTTTATTTATAATTAAGAATGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGa                                                                                                                                             >  2:302903/1‑141 (MQ=255)

   aaTGTCGGGCTTTTTTATTTTTAATTAAGAATGTAATAGTCTAACAATAAGCTATGTAAAGTATATAGCCTAGTTAATTATGCAAACGGATCGTTAGATTTATGCAGATAAAATTACAAATACTTTTCTTTTATTAGGAAg                                                                                                                                           >  2:458647/1‑141 (MQ=255)

    aTGTCGGGCTTTTTTATTTATAATTAAGAATGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAgg                                                                                                                                          <  1:897622/141‑1 (MQ=255)

        cGGGCTTTTTTATTTATAATTAAGAATGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTaaa                                                                                                                                      <  1:1042335/141‑1 (MQ=255)

        cGGGCTTTTTTATTTATAATTAAGAATGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTaaa                                                                                                                                      <  2:532290/141‑1 (MQ=255)

          ggCTTTTTTATTTATAATTAAGAATGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACg                                                                                                                                    <  2:890516/141‑1 (MQ=255)

            cTTTTTTATTTATAATTAAGAATGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTg                                                                                                                                  <  1:255312/141‑1 (MQ=255)

                    tttatAATTAAGAATGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGtta                                                                                                                          <  2:695865/141‑1 (MQ=255)

                       ataATTAAGAATGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGttatta                                                                                                                       <  2:139890/141‑1 (MQ=255)

                            tAAGAATGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACa                                                                                                                  <  1:815795/141‑1 (MQ=255)

                             aaGAATGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACAt                                                                                                                 <  1:843060/141‑1 (MQ=255)

                              aGAATGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATg                                                                                                                >  2:992629/1‑141 (MQ=255)

                              aGAATGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATg                                                                                                                >  2:170526/1‑141 (MQ=255)

                              aGAATGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATg                                                                                                                <  1:989627/141‑1 (MQ=255)

                              aGAATGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATg                                                                                                                <  1:418746/141‑1 (MQ=255)

                               gAATGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAAGAAGGCAAACGTGCCGTGTTATTATTACATgg                                                                                                               <  2:236759/141‑1 (MQ=255)

                                aaTGTAATAGTTTAACAATAAGCTATGTAAAATATATAGGCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGt                                                                                                              >  1:303433/1‑141 (MQ=255)

                                aaTGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGt                                                                                                              <  2:346955/141‑1 (MQ=255)

                                  tGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTANATGGtt‑t                                                                                                           >  2:815829/1‑141 (MQ=255)

                                  tGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGtt‑t                                                                                                           <  1:141115/141‑1 (MQ=255)

                                  tGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGtt‑t                                                                                                           <  1:182046/141‑1 (MQ=255)

                                   gTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGtt‑tt                                                                                                          <  2:1079332/141‑1 (MQ=255)

                                        aGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAgg                                                                                                     <  2:388708/141‑1 (MQ=255)

                                           ttAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGCGTTATTACTACATGGTT‑TTACAGGCaa                                                                                                  <  1:732883/141‑1 (MQ=255)

                                            tAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAAt                                                                                                 <  2:876587/141‑1 (MQ=255)

                                            tAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAAt                                                                                                 <  1:1037242/141‑1 (MQ=255)

                                            tAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAAt                                                                                                 <  1:568249/141‑1 (MQ=255)

                                             aaCAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTA‑ATATTTGTTACAGGCAAtt                                                                                                >  1:603808/1‑141 (MQ=255)

                                                aaTAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGTAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGt                                                                                             >  2:331368/1‑141 (MQ=255)

                                                aaTAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGt                                                                                             >  2:659412/1‑141 (MQ=255)

                                                   aaGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTg                                                                                          >  1:246712/1‑141 (MQ=255)

                                                     gCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGAt                                                                                        >  2:325608/1‑141 (MQ=255)

                                                      cTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTTTTTTTTTTTTAAGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATg                                                                                       >  2:1026289/1‑141 (MQ=255)

                                                       tATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGt                                                                                      <  2:387898/141‑1 (MQ=255)

                                                         tGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTc                                                                                    >  2:779756/1‑141 (MQ=255)

                                                         tGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTc                                                                                    >  1:501095/1‑141 (MQ=255)

                                                         tGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTc                                                                                    >  2:842588/1‑141 (MQ=255)

                                                         tGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTc                                                                                    >  1:325545/1‑141 (MQ=255)

                                                         tGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTAATACATGGTT‑TTACAGGCAATTCGTCTGGTGTTc                                                                                    >  1:325558/1‑141 (MQ=255)

                                                          gTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCg                                                                                   <  2:863824/141‑1 (MQ=255)

                                                               atatatAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAAt                                                                              <  1:1059044/141‑1 (MQ=255)

                                                                  tataGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAg                                                                           >  1:76335/1‑141 (MQ=255)

                                                                  tataGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAg                                                                           >  1:76351/1‑141 (MQ=255)

                                                                   ataGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCGATTAgg                                                                          <  1:964397/141‑1 (MQ=255)

                                                                    taGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGt                                                                         >  1:989647/1‑141 (MQ=255)

                                                                    taGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGt                                                                         >  2:845134/1‑141 (MQ=255)

                                                                      gCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCACAACCTTTATTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTAGTCTGATGTACGTCAATTAGGTCg                                                                       >  2:159022/1‑141 (MQ=255)

                                                                      gCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGTGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCg                                                                       >  2:345925/1‑141 (MQ=255)

                                                                      gCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAAGAAGGCAAACGTGCCGTGTTATTATTACATGGAT‑TTACAGGCAATTCGTCTGATGTACGTCAATTAGGTCg                                                                       >  2:78141/1‑141 (MQ=255)

                                                                      gCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAAGAAGGCAAACGTGCCGTGTTATTATTACATGGAT‑TTACAGGCAATTCGTCTGATGTACGTCAATTAGGTCg                                                                       >  2:78101/1‑141 (MQ=255)

                                                                        cTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGAt                                                                     <  2:846310/141‑1 (MQ=255)

                                                                         tAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGAtt                                                                    <  2:33945/141‑1 (MQ=255)

                                                                         tAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGAtt                                                                    <  2:852982/141‑1 (MQ=255)

                                                                         tAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGAtt                                                                    <  1:917331/141‑1 (MQ=255)

                                                                           gTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGAtttt                                                                  >  1:1011906/1‑141 (MQ=255)

                                                                              aaGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTAc                                                               <  1:958346/141‑1 (MQ=255)

                                                                              aaGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTAc                                                               <  1:443758/141‑1 (MQ=255)

                                                                               aGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTTTTTATTTTAATCGTAACGAGGTATTACTGCGTGGTT‑TTACAGGCAATTTGGTTGATGTTGGTGGTTTAGGTCGATTTTTaaa                                                              >  2:731571/1‑139 (MQ=255)

                                                                               aGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACa                                                              <  1:435208/141‑1 (MQ=255)

                                                                                gTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACaa                                                             >  1:127617/1‑141 (MQ=255)

                                                                                    gCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTTTTTTTTTTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAaaaga                                                         >  2:1015686/1‑141 (MQ=255)

                                                                                    gCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAaaaga                                                         <  2:385920/141‑1 (MQ=255)

                                                                                    gCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAaaaga                                                         <  2:699559/141‑1 (MQ=255)

                                                                                        aGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAgg                                                     <  2:391707/141‑1 (MQ=255)

                                                                                         gggAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGTGGTTTGTTAAGTTTGCGTGTTTTTATTATATGGTT‑TTACTGGCAATTCGTCTGATGTTCGTTAATTACGTCTATTTTTACAAGAGAAATGt                                                    >  2:74066/1‑141 (MQ=255)

                                                                                         gggAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGt                                                    <  2:400166/141‑1 (MQ=255)

                                                                                           gAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAAGAAGGCAAACGTGCCGTGTTATTATTACATGGAT‑TTACAGGCAATTCGTCTGATGTACGTCAATTAGGTCGATTTTTACaaaaaaagagata                                                  <  1:109092/141‑10 (MQ=255)

                                                                                             gCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTtata                                                <  1:716063/141‑1 (MQ=255)

                                                                                             gCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTtata                                                <  2:382280/141‑1 (MQ=255)

                                                                                               gTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTTTTTTTGTTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATAca                                              >  2:319000/1‑141 (MQ=255)

                                                                                                    aTTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCata                                         <  2:974823/141‑1 (MQ=255)

                                                                                                      ttATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATg                                       <  1:610850/141‑1 (MQ=255)

                                                                                                           cAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCg                                  <  1:722474/141‑1 (MQ=255)

                                                                                                           cAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTATAGTGTTATTACTACATGTTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAAAAAGGTTATACATCATATGCACCg                                  <  2:11662/141‑1 (MQ=255)

                                                                                                            aGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGc                                 <  2:104898/141‑1 (MQ=255)

                                                                                                               tAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAat                              >  2:345063/1‑141 (MQ=255)

                                                                                                                 aaaTTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAatat                            >  1:1075010/1‑141 (MQ=255)

                                                                                                                 aaaTTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAatat                            >  1:440928/1‑141 (MQ=255)

                                                                                                                    ttACCAAAACCTTTCTTTTTTTTTTTTTTTTAACGTGCAGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGGTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGATATACATCATATGTACTGCAATATGaa                         >  2:488527/1‑141 (MQ=255)

                                                                                                                      aCCAAAACCTTTCTTTTTTGAAGAAGGCAAACGTGCCGTGTTATTATTACATGGAT‑TTACAGGCAATTCGTCTGATGTACGTCAATTAGGTCGATTTTTACAAAAAAAGGGATATACTTCGTATGCACCACAATATGAAgg                       <  1:78101/141‑1 (MQ=255)

                                                                                                                       ccAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTGGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGc                      >  1:1083375/1‑141 (MQ=255)

                                                                                                                        cAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGcc                     <  1:425089/141‑1 (MQ=255)

                                                                                                                         aaaaCCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCa                    >  1:653824/1‑141 (MQ=255)

                                                                                                                         aaaaCCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCa                    >  1:109040/1‑141 (MQ=255)

                                                                                                                          aaaCCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCGATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCAc                   <  1:203537/141‑1 (MQ=255)

                                                                                                                          aaaCCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCAc                   >  1:514729/1‑141 (MQ=255)

                                                                                                                           aaCCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCAcg                  <  2:1042963/141‑1 (MQ=255)

                                                                                                                            aCCCTTCTTTTTTGAGGCAGGTAAACGTGCCGTGTTATTACTACGTGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCAcgc                 <  2:94109/141‑1 (MQ=255)

                                                                                                                               tttctttTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGc              <  2:400712/141‑1 (MQ=255)

                                                                                                                                ttctttTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGca             <  1:254814/141‑1 (MQ=255)

                                                                                                                                 tctttTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGcac            >  1:335442/1‑141 (MQ=255)

                                                                                                                                 tctttTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGcac            <  1:672670/141‑1 (MQ=255)

                                                                                                                                   ttttttGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGcacca          >  1:410589/1‑141 (MQ=255)

                                                                                                                                      tttGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCAccacca       <  1:794246/141‑1 (MQ=255)

                                                                                                                                        tGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGa     >  2:763046/1‑141 (MQ=255)

                                                                                                                                          aGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATg   <  1:717283/141‑1 (MQ=255)

                                                                                                                                          aGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATg   >  2:153131/1‑141 (MQ=255)

                                                                                                                                           ggAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGa  >  1:892535/1‑141 (MQ=255)

                                                                                                                                           ggAAGGTAAACGTCCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGa  <  1:177527/141‑1 (MQ=255)

GTAAATGTCGGGCTTTTTTATTTATAATTAAGAATGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGA  >  CP000730/864581‑864860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`