BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	864,726	T→C	5.7%	G14G (GGT→GGC)	est →	carboxylesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	864,726	0	T	C	5.7%	296.6 / 5.8	104	G14G (GGT→GGC)	est	carboxylesterase
Reads supporting (aligned to +/- strand): ref base T (43/55); new base C (2/4); total (45/59)
Fisher's exact test for biased strand distribution p-value = 6.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 105 total aligned reads displayed.

CGGGCTTTTTTATTTATAATTAAGAATGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATAC  >  CP000730/864589‑864865

cGGGCTTTTTTATTTATAATTAAGAATGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTaaa                                                                                                                                           <  1:1042335/141‑1 (MQ=255)

cGGGCTTTTTTATTTATAATTAAGAATGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTaaa                                                                                                                                           <  2:532290/141‑1 (MQ=255)

  ggCTTTTTTATTTATAATTAAGAATGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACg                                                                                                                                         <  2:890516/141‑1 (MQ=255)

    cTTTTTTATTTATAATTAAGAATGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTg                                                                                                                                       <  1:255312/141‑1 (MQ=255)

            tttatAATTAAGAATGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGtta                                                                                                                               <  2:695865/141‑1 (MQ=255)

               ataATTAAGAATGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGttatta                                                                                                                            <  2:139890/141‑1 (MQ=255)

                    tAAGAATGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACa                                                                                                                       <  1:815795/141‑1 (MQ=255)

                     aaGAATGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACAt                                                                                                                      <  1:843060/141‑1 (MQ=255)

                      aGAATGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATg                                                                                                                     >  2:992629/1‑141 (MQ=255)

                      aGAATGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATg                                                                                                                     <  1:418746/141‑1 (MQ=255)

                      aGAATGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATg                                                                                                                     <  1:989627/141‑1 (MQ=255)

                      aGAATGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATg                                                                                                                     >  2:170526/1‑141 (MQ=255)

                       gAATGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAAGAAGGCAAACGTGCCGTGTTATTATTACATgg                                                                                                                    <  2:236759/141‑1 (MQ=255)

                        aaTGTAATAGTTTAACAATAAGCTATGTAAAATATATAGGCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGt                                                                                                                   >  1:303433/1‑141 (MQ=255)

                        aaTGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGt                                                                                                                   <  2:346955/141‑1 (MQ=255)

                          tGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTANATGGtt‑t                                                                                                                >  2:815829/1‑141 (MQ=255)

                          tGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGtt‑t                                                                                                                <  1:141115/141‑1 (MQ=255)

                          tGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGtt‑t                                                                                                                <  1:182046/141‑1 (MQ=255)

                          tGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGtt‑t                                                                                                                >  2:578806/1‑141 (MQ=255)

                           gTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGtt‑tt                                                                                                               <  2:1079332/141‑1 (MQ=255)

                                aGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAgg                                                                                                          <  2:388708/141‑1 (MQ=255)

                                   ttAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGCGTTATTACTACATGGTT‑TTACAGGCaa                                                                                                       <  1:732883/141‑1 (MQ=255)

                                    tAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAAt                                                                                                      <  1:568249/141‑1 (MQ=255)

                                    tAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAAt                                                                                                      <  2:876587/141‑1 (MQ=255)

                                    tAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAAt                                                                                                      <  1:1037242/141‑1 (MQ=255)

                                     aaCAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTA‑ATATTTGTTACAGGCAAtt                                                                                                     >  1:603808/1‑141 (MQ=255)

                                        aaTAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGTAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGt                                                                                                  >  2:331368/1‑141 (MQ=255)

                                        aaTAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGt                                                                                                  >  2:659412/1‑141 (MQ=255)

                                           aaGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTg                                                                                               >  1:246712/1‑141 (MQ=255)

                                             gCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGAt                                                                                             >  2:325608/1‑141 (MQ=255)

                                              cTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTTTTTTTTTTTTAAGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATg                                                                                            >  2:1026289/1‑141 (MQ=255)

                                               tATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGt                                                                                           <  2:387898/141‑1 (MQ=255)

                                                 tGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTc                                                                                         >  1:325545/1‑141 (MQ=255)

                                                 tGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTc                                                                                         >  1:501095/1‑141 (MQ=255)

                                                 tGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTc                                                                                         >  2:842588/1‑141 (MQ=255)

                                                 tGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTc                                                                                         >  2:779756/1‑141 (MQ=255)

                                                 tGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTAATACATGGTT‑TTACAGGCAATTCGTCTGGTGTTc                                                                                         >  1:325558/1‑141 (MQ=255)

                                                  gTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCg                                                                                        <  2:863824/141‑1 (MQ=255)

                                                       atatatAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAAt                                                                                   <  1:1059044/141‑1 (MQ=255)

                                                          tataGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAg                                                                                >  1:76351/1‑141 (MQ=255)

                                                          tataGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAg                                                                                >  1:76335/1‑141 (MQ=255)

                                                           ataGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCGATTAgg                                                                               <  1:964397/141‑1 (MQ=255)

                                                            taGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGt                                                                              >  1:989647/1‑141 (MQ=255)

                                                            taGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGt                                                                              >  2:845134/1‑141 (MQ=255)

                                                              gCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGTGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCg                                                                            >  2:345925/1‑141 (MQ=255)

                                                              gCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCg                                                                            >  2:192552/1‑141 (MQ=255)

                                                              gCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAAGAAGGCAAACGTGCCGTGTTATTATTACATGGAT‑TTACAGGCAATTCGTCTGATGTACGTCAATTAGGTCg                                                                            >  2:78101/1‑141 (MQ=255)

                                                              gCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAAGAAGGCAAACGTGCCGTGTTATTATTACATGGAT‑TTACAGGCAATTCGTCTGATGTACGTCAATTAGGTCg                                                                            >  2:78141/1‑141 (MQ=255)

                                                                cTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGAt                                                                          <  2:846310/141‑1 (MQ=255)

                                                                 tAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGAtt                                                                         <  1:917331/141‑1 (MQ=255)

                                                                 tAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGAtt                                                                         <  2:852982/141‑1 (MQ=255)

                                                                 tAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGAtt                                                                         <  2:33945/141‑1 (MQ=255)

                                                                   gTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGAtttt                                                                       >  1:1011906/1‑141 (MQ=255)

                                                                      aaGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTAc                                                                    <  1:958346/141‑1 (MQ=255)

                                                                      aaGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTAc                                                                    <  1:443758/141‑1 (MQ=255)

                                                                       aGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTTTTTATTTTAATCGTAACGAGGTATTACTGCGTGGTT‑TTACAGGCAATTTGGTTGATGTTGGTGGTTTAGGTCGATTTTTaaa                                                                   >  2:731571/1‑139 (MQ=255)

                                                                       aGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACa                                                                   <  1:435208/141‑1 (MQ=255)

                                                                        gTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACaa                                                                  >  1:127617/1‑141 (MQ=255)

                                                                            gCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTTTTTTTTTTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAaaaga                                                              >  2:1015686/1‑141 (MQ=255)

                                                                            gCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAaaaga                                                              <  2:385920/141‑1 (MQ=255)

                                                                            gCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAaaaga                                                              <  2:699559/141‑1 (MQ=255)

                                                                                aGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAgg                                                          <  2:391707/141‑1 (MQ=255)

                                                                                 gggAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGTGGTTTGTTAAGTTTGCGTGTTTTTATTATATGGTT‑TTACTGGCAATTCGTCTGATGTTCGTTAATTACGTCTATTTTTACAAGAGAAATGt                                                         >  2:74066/1‑141 (MQ=255)

                                                                                 gggAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGt                                                         <  2:400166/141‑1 (MQ=255)

                                                                                   gAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAAGAAGGCAAACGTGCCGTGTTATTATTACATGGAT‑TTACAGGCAATTCGTCTGATGTACGTCAATTAGGTCGATTTTTACaaaaaaagagata                                                       <  1:109092/141‑10 (MQ=255)

                                                                                     gCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTtata                                                     <  1:716063/141‑1 (MQ=255)

                                                                                     gCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTtata                                                     <  2:382280/141‑1 (MQ=255)

                                                                                       gTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTTTTTTTGTTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATAca                                                   >  2:319000/1‑141 (MQ=255)

                                                                                            aTTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCata                                              <  2:974823/141‑1 (MQ=255)

                                                                                              ttATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATg                                            <  1:610850/141‑1 (MQ=255)

                                                                                                   cAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCg                                       <  1:722474/141‑1 (MQ=255)

                                                                                                   cAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTATAGTGTTATTACTACATGTTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAAAAAGGTTATACATCATATGCACCg                                       <  2:11662/141‑1 (MQ=255)

                                                                                                    aGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGc                                      <  2:104898/141‑1 (MQ=255)

                                                                                                       tAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAat                                   >  2:345063/1‑141 (MQ=255)

                                                                                                         aaaTTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAatat                                 >  1:1075010/1‑141 (MQ=255)

                                                                                                         aaaTTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAatat                                 >  1:440928/1‑141 (MQ=255)

                                                                                                            ttACCAAAACCTTTCTTTTTTTTTTTTTTTTAACGTGCAGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGGTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGATATACATCATATGTACTGCAATATGaa                              >  2:488527/1‑141 (MQ=255)

                                                                                                              aCCAAAACCTTTCTTTTTTGAAGAAGGCAAACGTGCCGTGTTATTATTACATGGAT‑TTACAGGCAATTCGTCTGATGTACGTCAATTAGGTCGATTTTTACAAAAAAAGGGATATACTTCGTATGCACCACAATATGAAgg                            <  1:78101/141‑1 (MQ=255)

                                                                                                               ccAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTGGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGc                           >  1:1083375/1‑141 (MQ=255)

                                                                                                                cAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGcc                          <  1:425089/141‑1 (MQ=255)

                                                                                                                 aaaaCCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCa                         >  1:653824/1‑141 (MQ=255)

                                                                                                                 aaaaCCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCa                         >  1:109040/1‑141 (MQ=255)

                                                                                                                  aaaCCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCGATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCAc                        <  1:203537/141‑1 (MQ=255)

                                                                                                                  aaaCCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCAc                        >  1:514729/1‑141 (MQ=255)

                                                                                                                   aaCCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCAcg                       <  2:1042963/141‑1 (MQ=255)

                                                                                                                    aCCCTTCTTTTTTGAGGCAGGTAAACGTGCCGTGTTATTACTACGTGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCAcgc                      <  2:94109/141‑1 (MQ=255)

                                                                                                                       tttctttTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGc                   <  2:400712/141‑1 (MQ=255)

                                                                                                                        ttctttTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGca                  <  1:254814/141‑1 (MQ=255)

                                                                                                                         tctttTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGcac                 >  1:335442/1‑141 (MQ=255)

                                                                                                                         tctttTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGcac                 <  1:672670/141‑1 (MQ=255)

                                                                                                                           ttttttGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGcacca               >  1:410589/1‑141 (MQ=255)

                                                                                                                              tttGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCAccacca            <  1:794246/141‑1 (MQ=255)

                                                                                                                                tGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGa          >  2:763046/1‑141 (MQ=255)

                                                                                                                                  aGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATg        >  2:153131/1‑141 (MQ=255)

                                                                                                                                  aGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATg        <  1:717283/141‑1 (MQ=255)

                                                                                                                                   ggAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGa       >  1:892535/1‑141 (MQ=255)

                                                                                                                                   ggAAGGTAAACGTCCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGa       <  1:177527/141‑1 (MQ=255)

                                                                                                                                    gAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGaa      <  1:89797/141‑1 (MQ=255)

                                                                                                                                    gAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGaa      <  1:89841/141‑1 (MQ=255)

                                                                                                                                        gTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATAc  <  2:142713/141‑1 (MQ=255)

CGGGCTTTTTTATTTATAATTAAGAATGTAATAGTTTAACAATAAGCTATGTAAAATATATAGCCTAGTTAAGTATGCAAAGGGAGCGTTAGATTTATGCAGATAAAATTACCAAAACCTTTCTTTTTTGAGGAAGGTAAACGTGCCGTGTTATTACTACATGGTT‑TTACAGGCAATTCGTCTGATGTTCGTCAATTAGGTCGATTTTTACAAAAGAAAGGTTATACATCATATGCACCGCAATATGAAGGCCACGCGGCACCACCAGATGAAATAC  >  CP000730/864589‑864865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`