BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	2,119,129	T→C	6.4%	M373V (ATG→GTG)	USA300HOU_1992 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,119,129	0	T	C	6.4%	303.9 / 7.6	104	M373V (ATG→GTG)	USA300HOU_1992	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (51/46); new base C (5/2); total (56/48)
Fisher's exact test for biased strand distribution p-value = 4.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 111 total aligned reads displayed.

ATTATTGTCATCCTCTAACTTCTCGATGATTAGCTTTATTTTCTTGCCTTCTGAAATAATGTGATTGATAGATGTTTGTATTGTTTCTAATTC‑TTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTAACTTTTGACCACAACAACTACATACATTGTCATCAAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTT  >  CP000730/2118989‑2119269

attattGTCATCCTCTAACTTCTCGATGATTAGCTTTATTTTCTTGCCTTCTGAAATAATGTGATTGATAGATGTTTGTATTGTTTCTAATTC‑TTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCat                                                                                                                                              >  2:132188/1‑141 (MQ=255)

attattGTCATCCTCTAACTTCTCGATGATTAGCTTTATTTTCTTGCCTTCTGAAATAATGTGATTGATAGATGTTTGTATTGTTTCTAATTC‑TTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCat                                                                                                                                              >  1:386621/1‑141 (MQ=255)

 ttattGTCATCCTCTAACTTCTCGATGATTAGCTTTATTTTCTTGCCTTCTGAAATAATGTGATTGATAGATGTTTGTATTGTTTCTAATTC‑TTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCata                                                                                                                                             >  2:493051/1‑141 (MQ=255)

  tattGTCATCCTCTAACTTCTCGATGATTAGCTTTATTTTCTTGCCTTCTGAAATAATGTGATTGATAGATGTTTGTATTGTTTCTAATTC‑TTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCatat                                                                                                                                            <  2:1029978/141‑1 (MQ=255)

   attGTCATCCTCTAACTTCTCGATGATTAGCTTTATTTTCTTGCCTTCTGAAATAATGTGATTGATAGATGTTTGTATTGTTTCTAATTC‑TTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATc                                                                                                                                           <  1:457084/141‑1 (MQ=255)

     tGTCATCCTCTCACTTCTCAATGATTAGCTTTATTTTCTTGCCTTCTGAAATAATGTGATTGATAGATGTTTGTATTGTTTCTAATTC‑TTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCa                                                                                                                                         <  2:318502/141‑1 (MQ=255)

     tGTCATCCTCTAACTTCTCGATGATTAGCTTTATTTTCTTGCCTTCTGAAATAATGTGATTGATAGATGTTTGTATTGTTTCTAATTC‑TTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCa                                                                                                                                         >  1:1017113/1‑141 (MQ=255)

      gTCATCCTCTAACTTCTCGATGATTAGCTTTATTTTCTTGCCTTCTGAAATAATGTGATTGATAGATGTTTGTATTGTTTCTAATTCTTTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCa                                                                                                                                         >  1:1046794/1‑141 (MQ=255)

      gTCATCCTCTAACTTCTCGATGATTAGCTTTATTTTCTTGCCTTCTGAAATAATGTGATTGATAGATGTTTGTATTGTTTCTAATTC‑TTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCAc                                                                                                                                        <  1:730174/141‑1 (MQ=255)

      gTCATCCTCTAACTTCTCGATGATTAGCTTTATTTTCTTGCCTTCTGAAATAATGTGATTGATAGATGTTTGTATTGTTTCTAATTC‑TTTCGATTTGCTAGCAGTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCAc                                                                                                                                        <  2:528330/141‑1 (MQ=255)

          tCCTCTAACTTCTCGATGATTAGCTTTATTTTCTTGCCTTCTGAAATAATGTGATTGATAGATGTTTGTATTGTTTCTAATTC‑TTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGt                                                                                                                                    >  1:207070/1‑141 (MQ=255)

            ctctAACTTCTCGATGATTAGCTTTATTTTCTTGCCTTCTGAAATAATGTGATTGATAGATGCTTGTATTGTTTCTAATTC‑TTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTc                                                                                                                                  <  2:844811/141‑1 (MQ=255)

                aaCTTCTCGATGATTAGCTTTATTTTCTTGCCTTCTGAAATTATGTGATTGATAGATGTTTGTATTGTTTCTAATTC‑TTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACt                                                                                                                              <  2:600202/141‑1 (MQ=255)

                aaCTTCTCGATGATTAGCTTTATTTTCTTGCCTTCTGAAATAATGTGATTGATAGATGTTTGTATTGTTTCTGATTC‑TTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACt                                                                                                                              <  1:508675/141‑1 (MQ=255)

                aaCTTCTCGATGATTAGCTTTATTTTCTTGCCTTCTGAAATAATGTGATTGATAGATGTTTGTATTGTTTCTAATTC‑TTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACt                                                                                                                              >  1:773443/1‑141 (MQ=255)

                  cTTCTCGATGATTAGCTTTATTTTCTTGCCTTCTGAAATAATGTGATTGATAGATGTTTGTATTGTTTCTAATTC‑TTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTgg                                                                                                                            <  2:1007532/141‑1 (MQ=255)

                   ttCTCGATGATTAGCTTTATTTTCTTGCCTTCTGAAATAATGTGATTGATAGATGTTTGTATTGTTTCTAATTC‑TTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGt                                                                                                                           <  1:66685/141‑1 (MQ=255)

                   ttCTCGATGATTAGCTTTATTTTCTTGCCTTCTGAAATAATGTGATTGATAGATGTTTGTATTGTTTCTAATTC‑TTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGt                                                                                                                           <  1:66595/141‑1 (MQ=255)

                   ttCTCGATGATTAGCTTTATTTTCTTGCCTTCTGAAATAATGTGATTGATAGATGTTTGTATTGTTTCTAATTC‑TTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGt                                                                                                                           <  2:643158/141‑1 (MQ=255)

                    tctcGATGATTAGCTTTATTTTCTTGCCTTCTGAAATAATGTGATTGATAGATGTTTGTATTGTTTCTAATTC‑TTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTa                                                                                                                          <  1:469768/141‑1 (MQ=255)

                    tctcGATGATTAGCTTTATTTTCTTGCCTTCTGAAATAATGTGATTGATAGATGTTTGTATTGTTTCTAATTC‑TTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTa                                                                                                                          <  1:469781/141‑1 (MQ=255)

                      tcGATGATTAGCTTTATTTTCTTGCCTTCTGAAATAATGTGATTGATAGATGTTTGTATTGTTTCTAATTC‑TTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTAAc                                                                                                                        >  2:730200/1‑141 (MQ=255)

                         atgatTGGCTTTATTTTCTTGCCTTCTGACATAATGTGATTGATAGATGTTTGTATTGTTTCTAATTC‑TTTCGATTTTTTTACATTGAATTTCTGTAAAGCTTTTTCTCTTGCCTCATTCACTTGTTCAGTTGGTAACtgt                                                                                                                     >  1:575017/1‑139 (MQ=255)

                         atgatTGGCTTTATTTTCTTGCCTTCTGAAATAATGTGATTGATAGATGTTTGTATTGTTTCTAATTC‑TTTCGATTTTTTTACATTGAATTTCTGTAAAGCTTTTTCTCTTGCCTCATTCACTTGTTCAGTTGGTAACtgt                                                                                                                     >  1:139581/1‑139 (MQ=255)

                         atgatTGGCTTTATTTTCTTGCCTTCTGAAATAATGTGATTGATAGATGTTTGTATTGTTTCTAATTC‑TTTCGATTTTTTTACATTGAATTTCTGTAAAGCTTTTTCTCTTGCCTCATTCACTTGTTCAGTTGGTAACtgt                                                                                                                     >  1:139559/1‑139 (MQ=255)

                         atgatTAGCTTTATTTTCTTGCCTTCTGAAATAATGTGATTGATAGATGTTTGTATTGTTTCTAATTC‑TTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTAACttt                                                                                                                     <  2:624067/141‑1 (MQ=255)

                         atgatTAGCTTTATTTTCTTGCCTTCTGAAATAATGTGATTGATAGATGTTTGTATTGTTTCTAATTC‑TTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTAACttt                                                                                                                     <  2:929045/141‑1 (MQ=255)

                            atTAGCTTTATTTTCTTGCCTTCTGAAATAATGTGATTGATAGATGTTTGTATTGTTTCTAATTC‑TTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTAACTTTTGa                                                                                                                  <  2:709104/141‑1 (MQ=255)

                            atTAGCTTTATTTTCTTGCCTTCTGAAATAATGTGATTGATAGATGTTTGTATTGTTTCTAATTC‑TTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTAACTTTTGa                                                                                                                  >  1:638909/1‑141 (MQ=255)

                             ttAGCTTTATTTTCTTGCCTTCTGAAATAATGTGATTGATAGATGTTTGTATTGTTTCTAATTC‑TTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTAACTTTTTAc                                                                                                                 <  2:321515/141‑1 (MQ=255)

                             ttAGCTTTATTTTCTTGCCTTCTGAAATAATGTGATTGATAGATGTTTGTATTGTTTCTAATTC‑TTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTAACTTTTGAc                                                                                                                 >  1:51040/1‑141 (MQ=255)

                               aGCTTTATTTTCTTGCCTTCTGAAATAATGTGATTGATAGATGTTTGTATTGTTTCTAATTC‑TTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTAACTTTTGACca                                                                                                               <  1:818779/141‑1 (MQ=255)

                                      ttttCTTGCCTTCTGAAATAATGTGATTGATAGATGTTTGTATTGTTTCTAATTC‑TTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTAACTTTTGACCACaacaac                                                                                                        <  2:384062/141‑1 (MQ=255)

                                      ttttCTTGCCTTCTGAAATAATGTGATTGATAGATGTTTGTATTGTTTCTAATTC‑TTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTAACTTTTGACCACaacaac                                                                                                        >  1:189716/1‑141 (MQ=255)

                                         tCTTGCCTTCTGAAATAATGTGATTGATAGATGTTTGTATTGTTTCTAATTC‑TTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTAACTTTTGACCACAACAACtac                                                                                                     >  2:735695/1‑141 (MQ=255)

                                          cTTGCCTTCTGAAATAATGTGATTGGTAGATGTTTGTATTGTTTCTAATTC‑TTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTAACTTTTGACCACAACAACtaca                                                                                                    >  2:513899/1‑141 (MQ=255)

                                          cTTGCCTTCTGAAATAATGTGATTGGTAGATGTTTGTATTGTTTCTAATTC‑TTTCGATTTGCTAGCATTGAATTTCTGAAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTAACTTTTGACCACAACAACtaca                                                                                                    >  2:575016/1‑141 (MQ=255)

                                          cTTGCCTTCTGAAATAATGTGATTGATAGATGTTTGTATTGTTTCTAATTC‑TTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTAACTTTTGACCACAACAACtaca                                                                                                    >  1:37001/1‑141 (MQ=255)

                                          cTTGCCTTCTGAAATAATGTGATTGATAGATGTTTGTATTGTTTCTAATTC‑TTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTAACTTTTGACCACAACAACtaca                                                                                                    >  1:651713/1‑141 (MQ=255)

                                           ttGCCTTCTGAAATAATGTGATTGATAGATGTTTGTATTGTTTCTAATTC‑TTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTAACTTTTGACCACAACAACtacat                                                                                                   <  2:309555/141‑1 (MQ=255)

                                           ttGCCTTCTGAAATAATGTGATTGATAGATGTTTGTATTGTTTCTAATTC‑TTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTAACTTTTGACCACAACAACtacat                                                                                                   >  2:590686/1‑141 (MQ=255)

                                            tGCCTTCTGAAATAATGTGATTGATAGATGTTTGTATTGTTTCTAATTC‑TTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTAACTTTTGACCACAACAACtacata                                                                                                  >  2:356409/1‑141 (MQ=255)

                                            tGCCTTCTGAAATAATGTGATCGATAGATGTTTGTATTGTTTCTAATTC‑TTTCGATTTGCTAGCATTGAATTTCTTCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTAACTTTTGACCACAACAACtacata                                                                                                  >  2:105298/1‑141 (MQ=255)

                                              ccTTCTGAAATAATGTGATTGATAGATGTTTGTATTGTTTCTAATTC‑TTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTAACTTTTGACCACAACAACtacataca                                                                                                <  2:663526/141‑1 (MQ=255)

                                                  cTGAAATAATGTGATTGATAGATGTTTGTATTGTTTCTAATTC‑TTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTAACTTTTGACCACAACAACTACATACATTGt                                                                                            >  1:793125/1‑141 (MQ=255)

                                                     aaaTAATGTGATTGATAGATGTTTGTATTGTTTCTAATTC‑TTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTAACTTTTGACCACAACAACTACATACATTGtcat                                                                                         >  1:570654/1‑141 (MQ=255)

                                                        taatGTGATTGATAGATGTTTGTATTGTTTCTAATTC‑TTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTAACTTTTGACCACAACAACTACATACATTGTCATCaa                                                                                      >  1:562083/1‑141 (MQ=255)

                                                        taatGTGATTGATAGATGTTTGTATTGTTTCTAATTC‑TTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTAACTTTTGACCACAACAACTACATACATTGTCATCaa                                                                                      >  2:319376/1‑141 (MQ=255)

                                                            gtgATTGATAGATGTTTGTATTGTTTCTAATTC‑TTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTAACTTTTGACCACAACAACTACATACATTGTCATCAAGata                                                                                  >  1:1081236/1‑141 (MQ=255)

                                                            gtgATTGATAGATGTTTGTATTGTTTCTAATTC‑TTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTAACTTTTGACCACAACAACTACATACATTGTCATCAAGata                                                                                  >  1:305454/1‑141 (MQ=255)

                                                             tgattgatAGATGTTTGTATTGTTTCTAATTC‑TTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTAACTTTTGACCACAACAACTACATACATTGTCATCAAGatat                                                                                 >  1:331390/1‑141 (MQ=255)

                                                             tgattgatAGATGTTTGTATTGTTTCTAATTC‑TTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTAACTTTTGACCACAACAACTACATACATTGTCATCAAGatat                                                                                 <  2:974177/141‑1 (MQ=255)

                                                             tgattgatAGATGTTTGTATTGTTTCTAATTC‑TTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTAACTTTTGACCACAACAACTACATACATTGTCATCAAGatat                                                                                 >  2:230411/1‑141 (MQ=255)

                                                              gattgatAGATGTTTGTATTGTTTCTAATTC‑TTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTAACTTTTGACCACNACAACTACATACNTTGTCATCAAGATAtt                                                                                <  1:988698/141‑1 (MQ=255)

                                                                 tgatAGATGTTTGTATTGTTTCTAATTC‑TTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTAACTTTTGACCACAACAACTACATACATTGTCATCAAGATATTCaa                                                                             >  2:705960/1‑141 (MQ=255)

                                                                    tAGATGTTTGTATTGTTTCTAATTC‑TTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTAACTTTTGACCACAACAACTACATACATTGTCATCAAGATATTCAAAtt                                                                          >  1:460475/1‑141 (MQ=255)

                                                                      gATGTTTGTATTGTTTCTAATTC‑TTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTAACTTTTGACCACAACAACTACATACATTGTCATCAAGATATTCAAAtttt                                                                        >  1:506157/1‑141 (MQ=255)

                                                                       aTGTTTGTATTGTTTCTAATTC‑TTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTCTCTCTTGCCACATCCACTTGTTCAACTGGTAACTTTTGACCACAACAACTACATACATTGTCATCAAGATATTCAAAttttt                                                                       <  1:102255/141‑1 (MQ=255)

                                                                        tGTTTGTATTGTTTCTAATTC‑TTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTTCCATATCCACTTGTTCAACTGGTAACTTTTGACCACAACAACTACATACATTGTCATCAAGATATTCAAATTTTTg                                                                      >  2:991182/1‑141 (MQ=255)

                                                                           ttGTATTGTTTCTAATTC‑TTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTAACTTTTGACCACAACAACTACATACATTGTCATCAAGATATTCAAATTTTTGAtt                                                                   <  2:490068/141‑1 (MQ=255)

                                                                           ttGTATTGTTTCTAATTC‑TTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTAACTTTTGACCACAACAACTACATACATTGTCATCAAGATATTCAAATTTTTGAtt                                                                   >  1:1077523/1‑141 (MQ=255)

                                                                            tGTATTGTTTCTAATTC‑TTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTAACTTTTGACCACAACAACTACATACATTGTCATCAAGATATTCAAATTTTTGAttt                                                                  >  2:307713/1‑141 (MQ=255)

                                                                             gTATTGTTTCTAATTC‑TTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTAACTTTTGACCACAACAACTACATACATTGTCATCAAGATATTCAAATTTTTGAtttt                                                                 <  1:466325/141‑1 (MQ=255)

                                                                             gTATTGTTTCTAATTC‑TTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTAACTTTTGACCACAACAACTACATACATTGTCATCAAGATATTCAAATTTTTGAtttt                                                                 <  2:927506/141‑1 (MQ=255)

                                                                             gTATTGTTTCTAATTC‑TTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTAACTTTTGACCACAACAACTACATACATTGTCATCAAGATATTCAAATTTTTGAtttt                                                                 >  2:49198/1‑141 (MQ=255)

                                                                                  gTTTCTAATTC‑TTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTAACTTTTGACCACAACAACTACATACATTGTCATCAAGATATTCAAATTTTTGATTTTTAGCt                                                            <  1:85345/141‑1 (MQ=255)

                                                                                   tttCTAATTC‑TTTCGATTTGTTTGCATTGAATTTCTGCAATGCTTTTTCTCTTACCTCACTCACTTGTTCAGCTGGTAACTGTTGACCACAACAACTACATACATTGTCATCAAGATATTCAAATTTTTGATTTTTAGCtt                                                           >  1:89043/1‑141 (MQ=255)

                                                                                     tCTAATTC‑TTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTAACTTTTGACCACAACAACTACATACATTGTCATCAAGATATTCAAATTTTTGATTTTTAGCtttt                                                         >  1:275619/1‑141 (MQ=255)

                                                                                       tAATTC‑TTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTAACTTTTGACCACAACAACTACATACATTGTCATCAAGATATTCAAATTTTTGATTTTTAGCTTTTTc                                                       <  2:1082495/141‑1 (MQ=255)

                                                                                         aTTC‑TTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTAACTTTTGACCACAACAACTACATACATTGTCATCAAGATATTCAAATTTTTGATTTTTAGCTTTTTCTa                                                     >  1:550576/1‑141 (MQ=255)

                                                                                          ttC‑TTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTAACTTTTGACCACAACAACTACATACATTGTCATCAAGATATTCAAATTTTTGATTTTTAGCTTTTTCTaa                                                    <  1:839143/141‑1 (MQ=255)

                                                                                                tCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTAACTTTTGACCACAACAACTACATACATTGTCATCAAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCAc                                               >  1:743258/1‑141 (MQ=255)

                                                                                                   aTTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTAACTTTTGACCACAACAACTACATACATTGTCATCAAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACttt                                            <  2:171398/141‑1 (MQ=255)

                                                                                                    tttgtttgCATTGAATTTCTGCAATGCTTTTTCTCTTACCTCACTCACTTGTTCAGCTGGTAACTGTTGACCACAACAACTACATACATTGTCATCAAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACtttt                                           >  2:33652/8‑141 (MQ=255)

                                                                                                       gCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTAACTTTTGACCACAACAACTACATACATTGTCATCAAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTaa                                        >  1:286099/1‑141 (MQ=255)

                                                                                                       gCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTAACTTTTGACCACAACAACTACATACATTGTCATCAAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTaa                                        >  1:888165/1‑141 (MQ=255)

                                                                                                        cTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTAACTTTTGACCACAACAACTACATACATTGTCATCAAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAAt                                       >  2:766900/1‑141 (MQ=255)

                                                                                                        cTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTAACTTTTGACCACAACAACTACATACATTGTCATCAAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAAt                                       <  1:733253/141‑1 (MQ=255)

                                                                                                          aGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTAACTTTTGACCACAACAACTACATACATTGTCATCAAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATcc                                     <  2:18783/141‑1 (MQ=255)

                                                                                                           gCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTAACTTTTGACCACAACAACTACATACATGGGCATCAAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTCTTTAATCCt                                    >  1:1053763/1‑141 (MQ=255)

                                                                                                            cattgcagaaattcAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTAACTTTTGACCACAACAACTACATACATTGTCATCAAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCtt                                   >  2:92741/14‑141 (MQ=255)

                                                                                                            cATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTAACTTTTGACCACAACAACTACATACATTGTCATCAAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCtt                                   <  2:972800/141‑1 (MQ=255)

                                                                                                             aTTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTAACTTTTGACCACAACAACTACATACATTGTCATCAAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCttt                                  <  1:983598/141‑1 (MQ=255)

                                                                                                              ttGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTAACTTTTGACCACAACAACTACATACATTGTCATCAAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTa                                 <  2:207070/141‑1 (MQ=255)

                                                                                                                  aTTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTAACTTTTGACCACAACAACTACATACATTGTCATCAAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGAt                             >  1:435692/1‑141 (MQ=255)

                                                                                                                     tCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTAACTTTTGACCACAACAACTACATACATTGTCATCAAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATAAAATTTTAATCCTTTATGAtttt                          >  1:9049/1‑141 (MQ=255)

                                                                                                                      cTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTAACTTTTGACCACAACAACTACATACATTGTCATCAAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTc                         >  1:770709/1‑141 (MQ=255)

                                                                                                                      cTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTAACTTTTGACCACAACAACTACATACATTGTCATCAAGATATTCAAATTTTTGATTTTTAGATTTTTCTTTTTTACTTTTTAATACTTTATTAttttt                         >  2:467720/1‑140 (MQ=255)

                                                                                                                       tGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTAACTTTTGACCACAACAACTACATACATTGTCATCAAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCt                        <  2:573043/141‑1 (MQ=255)

                                                                                                                           aTGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTAACTTTTGACCACAACAACTACATACATTGTCATCAAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCtaata                    <  1:600715/141‑1 (MQ=255)

                                                                                                                                   tctcTTGCCATATCCACTTGTTCAACTGGTAACTTTTGACCACAACAACTACATACATTGTCATCAAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTa            >  1:212249/1‑141 (MQ=255)

                                                                                                                                   tctcTTGCCATATCCACTTGTTCAACTGGTAACTTTTGACCACAACAACTACATACATTGTCATCAAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTa            >  2:409616/1‑141 (MQ=255)

                                                                                                                                     tctTGCCATATCCACTTGTTCAACTGGTAACTTTTGACCACAACAACTACATACATTGTCATCAAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACg          <  1:936679/141‑1 (MQ=255)

                                                                                                                                       ttGCCATATCCACTTGTTCAACTGGTAACTTTTGACCACAACAACTACATACATTGTCATCAAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTc        <  1:951531/141‑1 (MQ=255)

                                                                                                                                        tGCCATATCCACTTGTTCAACTGGTAACTTTTGACCACAACAACTACATACATTGTCATCAAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCt       <  1:944501/141‑1 (MQ=255)

                                                                                                                                        tGCCATATCCACTTGTTCAACTGGTAACTTTTGACCACAACAACTACATACATTGTCATCAAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCt       >  2:223611/1‑141 (MQ=255)

                                                                                                                                          ccATATCCACTTGTTCAACTGGTAACTTTTGACCACAACAACTACATACATTGTCATCAAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCttt     <  2:973203/141‑3 (MQ=255)

                                                                                                                                          ccATATCCACTTGTTCAACTGGTAACTTTTGACCACAACAACTACATACATTGTCATCAAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTAt     <  2:506157/141‑1 (MQ=255)

                                                                                                                                            atatCCACTTGTTCAACTGGTAACTTTTGACCACAACAACTACATACATTGTCATCAAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTAttt   <  1:514260/141‑1 (MQ=255)

                                                                                                                                             tatCCACTTGTTCAACTGGTAACTTTTGACCACAACAACTACATACATTGTCATCAAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTAtttt  >  1:184876/1‑141 (MQ=255)

ATTATTGTCATCCTCTAACTTCTCGATGATTAGCTTTATTTTCTTGCCTTCTGAAATAATGTGATTGATAGATGTTTGTATTGTTTCTAATTC‑TTTCGATTTGCTAGCATTGAATTTCTGCAATGCTTTTTCTCTTGCCATATCCACTTGTTCAACTGGTAACTTTTGACCACAACAACTACATACATTGTCATCAAGATATTCAAATTTTTGATTTTTAGCTTTTTCTAAATCACTTTTTAATCCTTTATGATTTTCTAATAATTGATTACGTCTATTTT  >  CP000730/2118989‑2119269

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`