| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 2,559,840 | T→G | 10.5% | intergenic (+34/+89) | bcr → / ← USA300HOU_2421 | MFS family major facilitator transporter, bicyclomycin:cation symporter/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 2,559,840 | 0 | T | G | 10.5% | 95.5 / 5.0 | 38 | intergenic (+34/+89) | bcr/USA300HOU_2421 | MFS family major facilitator transporter, bicyclomycin:cation symporter/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (15/19); new base G (2/2); total (17/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
TTAATACATCACCATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACAT‑AATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAATATATTTTTCACTTGTAGT > CP000730/2559703‑2559979 | ttAATACATCACCATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACAT‑AATTAGAACCCTAGCAAAGAtatcta > 2:265746/1‑141 (MQ=255)ttAATACATCACCATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACAT‑AATTAGAACCCTAGCAAAGAtatcta > 2:127013/1‑141 (MQ=255) cATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACATAAAGAAGAACTCTGACAAAGATAGATATCTTTgctagg < 2:384784/141‑6 (MQ=37) cATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACAT‑AATTAGAACCCTAGCAAAGATATCTATCTTTGTCAggg < 1:87745/141‑1 (MQ=255) atatGATTATTATCTTCATTACAGCCATTCTATTAGTCATTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACAT‑AATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGttc < 1:452545/141‑1 (MQ=255) atatGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACAT‑AATTAGAACCCTGACAAAGATAGATATCTTTGCTAGGGttc < 1:203153/141‑1 (MQ=38) tattatCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACAT‑AATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTa < 1:176700/141‑1 (MQ=255) tACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACAT‑AATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATgaga < 2:286180/141‑1 (MQ=255) tACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACAT‑AATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATgaga < 1:236682/141‑1 (MQ=255) aGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACAT‑AATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCg > 1:13893/1‑141 (MQ=255) aGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACAT‑AATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCg > 1:168462/1‑141 (MQ=255) ttAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACAT‑AATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAAc < 1:223662/141‑1 (MQ=255) ttAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACAT‑AATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAAc < 1:35523/141‑1 (MQ=255) tcTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACAT‑AATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTa > 1:92644/1‑141 (MQ=255) aCAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACAT‑AATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGc > 1:160151/1‑141 (MQ=255) attttaaagtaaaTGATTAAAAAGCAACATGTCGCATAACACTTCAACAT‑AATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATa > 2:318486/11‑141 (MQ=255) tttACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACAT‑AATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAg > 2:410138/1‑141 (MQ=255) tACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACAT‑AATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCa > 1:399500/1‑141 (MQ=255) tttAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACAT‑AATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAgg < 2:70014/141‑1 (MQ=255) tttAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACAT‑AATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAgg < 1:305002/141‑1 (MQ=255) tAAAAAGATTAAAAACCAACATGTCACATAACACTACAACAT‑TATTAGACCCCTAGCAAAGATATCTATCTTTGTCAGGTTTCTTCTTTATGAATTATAAGATCGAATCTTCAACTAAAATTACGCCTTCCTAGCAAAGaa < 2:271034/141‑2 (MQ=255) aTTAAAAAGCAACATGTCGCATAACACTTCAACAT‑AATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTa > 1:273222/1‑141 (MQ=255) aTTAAAAAGCAACATGTCGCATAACACTTCAACAT‑AATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTa > 1:300156/1‑141 (MQ=255) ttAAAAATCAACATTTCACATAACACTTCAACAT‑AATTAGTACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATTGAAGCTGCAACTAATTTTACGCCTTCATAGCAAGGACATTTCTAt < 2:339710/141‑1 (MQ=255) cAACATGTCGCATAACACTTCAACAT‑AATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCAc > 2:23195/1‑141 (MQ=255) aaCATGTCGCATAACACTTCAACAT‑AATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCAcc > 1:83329/1‑141 (MQ=255) cGCATAACACTTCAACAT‑AATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAAc < 2:154374/141‑1 (MQ=255) tAACACTTCAACAT‑AATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGc < 2:312362/141‑1 (MQ=255) aaCACTTCAACAT‑AATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCa > 1:410522/1‑141 (MQ=255) acacTTCAACAT‑AATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCAt < 1:143637/141‑1 (MQ=255) cTTCAACAT‑AATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCa > 2:117044/1‑141 (MQ=255) aTTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTa > 1:263315/1‑141 (MQ=255) ttAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTaa < 2:227239/141‑1 (MQ=255) ttAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTaa < 2:227231/141‑1 (MQ=255) tAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTaa < 2:147307/119‑1 (MQ=255) tAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTaa > 1:147307/1‑119 (MQ=255) cctgacAAAGATAGATATCTTTGCTAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAATATAttt > 2:103547/6‑141 (MQ=255) ctgacAAAGATAGATATCTTTGCTAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAATATAtttt > 2:418122/5‑141 (MQ=255) aGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAATATATTTTTCACTTg < 1:301212/141‑1 (MQ=255) aGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGAGCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAATATATTTTTCACTTg < 1:86030/141‑1 (MQ=255) atctatctTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAATATATTTTTCACTTgtagt < 1:67787/141‑1 (MQ=255) | TTAATACATCACCATATATGATTATTATCTTCATTACAGCCATTCTATTAGTCAGTCTACAAATCATTTACTTTAAAATGATTAAAAAGCAACATGTCGCATAACACTTCAACAT‑AATTAGAACCCTAGCAAAGATATCTATCTTTGTCAGGGTTCTTCTTTATGAATTATGAGATCGAATCTTCAACTAAAATTACGCCTTCATAGCAAGGACATTTCTATTCAATCACCCTTTAACAGGCATCCAAATTTCTGTAATATATTTTTCACTTGTAGT > CP000730/2559703‑2559979 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |