BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	35,970	T→C	52.1%	E194E (GAA→GAG)	tpn ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	35,970	0	T	C	52.1%	23.0 / 280.7	211	E194E (GAA→GAG)	tpn	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base T (48/53); new base C (45/65); total (93/118)
Fisher's exact test for biased strand distribution p-value = 4.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.02e-01

Only 100 of 211 total aligned reads displayed.

CAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAA  >  CP000730/35867‑36107

cAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATAc                                                                                                      >  1:361001/1‑141 (MQ=11)

  gtgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACtt                                                                                                    >  2:210314/1‑141 (MQ=17)

  gtgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACtt                                                                                                    >  1:4772/1‑141 (MQ=12)

  gtgtTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACtt                                                                                                    >  1:276953/1‑141 (MQ=17)

  gtgtTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATCGATACtt                                                                                                    >  2:179274/1‑141 (MQ=17)

   tgtTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACttt                                                                                                   >  2:307769/1‑141 (MQ=32)

      tcgtttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGAt                                                                                                <  2:336890/137‑1 (MQ=11)

        gtttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAc                                                                                              <  2:296195/139‑1 (MQ=17)

         cTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAcc                                                                                             <  2:68605/141‑1 (MQ=32)

         cTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAcc                                                                                             <  1:217000/141‑1 (MQ=17)

          ttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGGAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCt                                                                                            <  2:15264/141‑1 (MQ=12)

          ttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCt                                                                                            <  2:389943/141‑1 (MQ=17)

          ttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCt                                                                                            <  2:415735/141‑1 (MQ=17)

           tAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCtt                                                                                           <  1:228458/141‑1 (MQ=17)

            aaCTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTg                                                                                          <  2:163176/141‑1 (MQ=11)

            aaCTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTg                                                                                          <  1:191713/141‑1 (MQ=11)

              cTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtc                                                                                        >  1:94391/1‑141 (MQ=32)

              cTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtc                                                                                        <  2:249247/141‑1 (MQ=32)

              cTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtc                                                                                        >  1:382687/1‑141 (MQ=17)

              cTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtc                                                                                        >  2:264854/1‑141 (MQ=17)

               ttGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtct                                                                                       <  1:98468/141‑1 (MQ=32)

                 gCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtcttt                                                                                     <  2:404609/141‑1 (MQ=32)

                 gCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACATGCGGTTC‑TCTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtcttt                                                                                     >  2:343059/1‑141 (MQ=17)

                   tAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttct                                                                                   >  1:143124/1‑141 (MQ=17)

                   tAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttct                                                                                   <  2:157384/141‑1 (MQ=17)

                   tAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttct                                                                                   >  1:163482/1‑141 (MQ=17)

                     gCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCTGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTa                                                                                 <  1:435523/141‑1 (MQ=17)

                       atgatgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACt                                                                               <  1:341162/141‑1 (MQ=21)

                         gatgCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACttt                                                                             <  2:14767/141‑1 (MQ=11)

                         gatgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACttt                                                                             >  2:83242/1‑141 (MQ=25)

                             cTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAata                                                                         >  1:241221/1‑141 (MQ=34)

                             cTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAata                                                                         <  1:328834/141‑1 (MQ=25)

                               aaTTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATg                                                                       <  1:112727/141‑1 (MQ=25)

                                aTTTCGTGGGATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGTGCGTAAATACACTCATTACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAatgtat                                                                      <  2:413076/141‑5 (MQ=11)

                                aTTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGa                                                                      <  2:408625/141‑1 (MQ=11)

                                aTTTCGTAGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGa                                                                      >  1:401850/1‑141 (MQ=25)

                                  ttCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACg                                                                    >  1:428362/1‑141 (MQ=11)

                                  ttCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACg                                                                    >  1:396185/1‑141 (MQ=37)

                                    cGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGt                                                                  <  2:195661/141‑1 (MQ=38)

                                     gTGGCGTGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTC‑TTTTTATATATAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTTCTTTAATATGACGGTg                                                                 >  2:104097/1‑141 (MQ=11)

                                       ggCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGAt                                                               >  1:201518/1‑141 (MQ=14)

                                       ggCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAt                                                               >  1:249947/1‑141 (MQ=255)

                                        gCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATc                                                              <  1:425323/141‑1 (MQ=255)

                                          aTGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCtt                                                            <  2:281028/141‑1 (MQ=14)

                                              ggAAAATCCGTAGATCTGAAGAGACCTACGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTc                                                        >  2:127302/2‑141 (MQ=255)

                                              cGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCt                                                         >  2:218087/1‑141 (MQ=255)

                                                 aaaTCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAAt                                                     <  1:264873/141‑1 (MQ=255)

                                                  aaTCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATg                                                    <  1:264281/141‑1 (MQ=39)

                                                  aaTCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATg                                                    <  1:354087/141‑1 (MQ=255)

                                                  aaTCCGTAGATCTGAAGAGACCTACGGTTC‑TTTTTATATAGAGGGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATg                                                    <  2:425333/141‑1 (MQ=255)

                                                    tCCGTAGATCTGAAGAGACCTACGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAg                                                  <  1:301632/141‑1 (MQ=255)

                                                     ccGTAGATCTGAAGAGACCTACGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAgg                                                 <  2:135143/141‑1 (MQ=255)

                                                      cGTAGATCTGACGAGACCTGAGGTTC‑TTTTTATATAGAACGTACATACACTCAATACCTTTTAAAGTATTCATTGCTGTATTGCTACTTTGATACCTTGTCTTTCTTACTTTCATATGACGGTGATTTTGCTTAATGAGGt                                                >  2:181552/1‑141 (MQ=11)

                                                      cGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGt                                                <  1:62800/141‑1 (MQ=255)

                                                      cGTAGATCTGAAGAGACCTACGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGACACTTTGATATCTTGTCTTTCTTACTTTAATATGTCGGTGATCTTGCTCAATGAGGt                                                <  2:446527/141‑1 (MQ=14)

                                                        tAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTa                                              >  1:232537/1‑141 (MQ=255)

                                                        tAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTa                                              >  2:293689/1‑141 (MQ=37)

                                                        tAGATCTGAAGAGACCTACGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTa                                              >  1:100003/1‑141 (MQ=255)

                                                        tAGATCTGAAGAGACCTACGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTACTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTa                                              <  1:119370/141‑1 (MQ=14)

                                                         aGATCTGAAGAGACCTACGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTAt                                             >  2:80256/1‑141 (MQ=255)

                                                             cTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAg                                         <  1:255744/141‑1 (MQ=39)

                                                             cTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAg                                         <  1:289920/141‑1 (MQ=255)

                                                             cTGAAGAGACCTACGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAg                                         <  2:49144/141‑1 (MQ=255)

                                                              tGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGa                                        <  2:142617/141‑1 (MQ=38)

                                                              tGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGa                                        <  2:318029/141‑1 (MQ=39)

                                                              tGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGa                                        <  1:281470/141‑1 (MQ=255)

                                                              tGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACGTTAATATGACGGTGTTCTTGCTCAATGAGGTTATTCAGa                                        <  2:40343/141‑1 (MQ=255)

                                                                aaGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGata                                      <  2:378833/141‑1 (MQ=255)

                                                                   agaCCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATAttt                                   >  2:440213/1‑141 (MQ=38)

                                                                    gaCCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTc                                  <  1:169028/141‑1 (MQ=255)

                                                                     aCCTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCg                                 <  1:371630/141‑1 (MQ=255)

                                                                      ccTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGa                                >  1:447716/1‑141 (MQ=255)

                                                                       cTGCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTCTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGAt                               <  2:371878/141‑1 (MQ=255)

                                                                         gCGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGt                             <  2:199625/141‑1 (MQ=38)

                                                                         gCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTTATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGt                             >  1:71650/1‑141 (MQ=255)

                                                                         gCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGt                             <  2:35364/141‑1 (MQ=255)

                                                                         gCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGt                             <  1:376278/141‑1 (MQ=38)

                                                                         gCGGTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGt                             >  2:253412/1‑141 (MQ=255)

                                                                         acGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGt                             >  1:197855/2‑141 (MQ=255)

                                                                         acGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGt                             >  2:53076/2‑141 (MQ=255)

                                                                            gTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACa                          >  2:174129/1‑141 (MQ=39)

                                                                            gTTC‑TTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACa                          <  2:449168/141‑1 (MQ=255)

                                                                                 tttttATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGa                      >  1:368612/1‑141 (MQ=255)

                                                                                  ttttATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGAc                     >  1:224610/1‑141 (MQ=255)

                                                                                   tttATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACa                    <  1:126533/141‑1 (MQ=255)

                                                                                     tatataGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGt                  <  2:2395/141‑1 (MQ=255)

                                                                                     tatataGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCGTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGt                  <  2:452138/141‑1 (MQ=255)

                                                                                       tataGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCa                <  1:305281/141‑1 (MQ=255)

                                                                                         taGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAgg              <  2:230361/141‑1 (MQ=255)

                                                                                         taGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAgg              <  1:31209/141‑1 (MQ=255)

                                                                                          agagCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgt             >  2:118389/1‑141 (MQ=255)

                                                                                          agagCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgt             >  1:110405/1‑141 (MQ=255)

                                                                                           gagCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtt            >  2:141118/1‑141 (MQ=255)

                                                                                           gagCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtt            >  1:337501/1‑141 (MQ=40)

                                                                                             gCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgttta          <  1:276421/141‑1 (MQ=255)

                                                                                             gCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgttta          <  1:187968/141‑1 (MQ=255)

                                                                                                  aaTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagttt     <  1:105446/141‑1 (MQ=255)

                                                                                                  aaTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgattaagttt     >  2:365608/1‑141 (MQ=255)

                                                                                                   aTACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagttta    >  1:206835/1‑141 (MQ=255)

                                                                                                    tACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagtttaa   <  1:135256/141‑1 (MQ=255)

CAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTC‑TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAA  >  CP000730/35867‑36107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`