Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	1,891,904	(T)6→5	coding (103/501 nt)	rot ←	repressor of toxins Rot

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,891,899	0	T	.	97.5%	177.4 / ‑1.7	40	coding (108/501 nt)	rot	repressor of toxins Rot
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base . (19/20);  total (20/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.01e-01

TTTTGCCATAAAGTTAGTAAAATTAAAATTTCTTCTCTAGACATTTTGTATTCGCTTTCAATCTCGCTGAAAATTGAGTTAATGTCACCCAAAAGTGTTTCTAATTGCAAAATTCCAAATACAGTGTCGTTATTTACTTTTTTCATGCTAAACATCTCCCAATTAATAATCACATATTTAGCTACTATACAAGTATTAACAAACATCCCAACAATCCCAAAACTTGTATGTGCTAACTTATGCATAAAACTACAAGTGTAAATAAA  >  CP000730/1891762‑1892027                                                                                                                                          |                                                                                                                                 ttttGCCATAAAGTTAGTAAAATTAAAATTTCTTCTCTAGACATTTTGTATTCGCTTTCAATCTCGCTGAAAATTGAGTTAATGTCACCCAAAAGTGTTTCTAATTGCAAAATTCCAAATACAGTGTCGTTATTTACtttt                                                                                                                               >  2:156476/1‑141 (MQ=255)     gCCATAAAGTTAGTAAAATTAAAATTTCTTCTCTAGACATTTTGTATTCGCTTTCAATCTCGCTGAAAATTGAGTTAATGTCACCCAAAAGTGTTTCTAATTGCAAAATCCCAAATACAGTGTCGTTATTTACTTTTTTCa                                                                                                                           >  1:350218/1‑141 (MQ=255)        aTAAAGTTAGTAAAATTAAAATTTCTTCTCTAGACATTTTGTATTCGCTTTCAATCTCGCTGAAAATTGAGTTAATGTCACCCAAAAGTGTTTCTAATTGCAAAATTCCAAATACAGTGTCGTTATTTAC‑TTTTTCATGCt                                                                                                                       >  1:98349/1‑141 (MQ=255)        aTAAAGTTAGTAAAATTAAAATTTCTTCTCTAGACATTTTGTATTCGCTTTCAATCTCGCTGAAAATTGAGTTAATGTCACCCAAAAGTGTTTCTAATTGCAAAATTCCAAATACAGTGTCGTTATTTAC‑TTTNTCATGCt                                                                                                                       >  2:310426/1‑141 (MQ=255)                      attaaaattTCTTCTCTAGACATTTTGTATTCGCTTTCAATCTCGCTGAAAATTGAGTTAATGTCACCCAAAAGTGTTTCTAATTGCAAAATTCCAAATACAGTGTCGTTATTTAC‑TTTTTCATGCTAAACATCTCCCAAt                                                                                                         >  2:373073/1‑141 (MQ=255)                       ttaaaattTCTTCTCTAGACATTTTGTATTCGCTTTCAATCTCGCTGAAAATTGAGTTAATGTCACCCAAAAGTGTTTCTAATTGCAAAATTCCACATACAGTGTCGTTATTTAC‑TTTTTCATGCTAAACATCTCCCAAtt                                                                                                        <  2:413501/141‑1 (MQ=255)                          aaattTCTTCTCTAGACATTTTGTATTCGCTTTCAATCTCGCTGAAAATTGAGTTAATGTCACCCAAAAGTGTTTCTAATTGCAAAATTCCAAATACAGTGTCGTTATTTAC‑TTTTTCATGCTAAACATCTCCCAATtaat                                                                                                     <  2:379354/141‑1 (MQ=255)                          aaattTCTTCTCTAGACATTTTGTATTCGCTTTCAATCTCGCTGAAAATTGAGTTAATGTCACCCAAAAGTGTTTCTAATTGCAAAATTCCAAATACAGTGTCGTTATTTAC‑TTTTTCATGCTAAACATCTCCCAATtaat                                                                                                     <  1:183166/141‑1 (MQ=255)                          aaattTCTTCTCTAGACATTTTGTATTCGCTTTCAATCTCGCTGAAAATTGAGTTAATGTCACCCAAAAGTGTTTCTAATTGCAAAATTCCAAATACAGTGTCGTTATTTAC‑TTTTTCATGCTAAACATCTCCCAATtaat                                                                                                     <  1:389068/141‑1 (MQ=255)                               tcttctCTAGACATTTTGTATTCGCTTTCAATCTCGCTGAAAATTGAGTTAATGTCACCCAAAAGTGTTTCTAATTGCAAAATTCCAAATACAGTGTCGTTATTTAC‑TTTTTCATGCTAAACATCTCCCAATTAATAATca                                                                                                <  2:231872/141‑1 (MQ=255)                               tcttctCTAGACATTTTGTATTCGCTTTCAATCTCGCTGAAAATTGAGTTAATGTCACCCAAAAGTGTTTCTAATTGCAAAATTCCAAATACAGTGTCGTTATTTAC‑TTTTTCATGCTAAACATCTCCCAATTAATAATca                                                                                                <  2:52514/141‑1 (MQ=255)                                cttctCTAGACATTTTGTATTCGCTTTCAATCTCGCTGAAAATTGAGTTAATGTCACCCAAAAGTGTTTCTAATTGCAAAATTCCAAATACAGTGTCGTTATTTAC‑TTTTTCATGCTAAACATCTCCCAATTAATAATcac                                                                                               <  2:98385/141‑1 (MQ=255)                                          cATTTTGTATTCGCTTTCAATCTCGCTGAAAATTGAGTTAATGTCACCCAAAAGTGTTTCTAATTGCAAAATTCCAAATACAGTGTCGTTATTTAC‑TTTTTCATGCTAAACATCTCCCAATTAATAATCACATATTTAGct                                                                                     >  1:27183/1‑141 (MQ=255)                                          cATTTTGTATTCGCTTTCAATCTCGCTGAAAATTGAGTTAATGTCACCCAAAAGTGTTTCTAATTGCAAAATTCCAAATACAGTGTCGTTATTTAC‑TTTTTCATGCTAAACATCTCCCAATTAATAATCACATATTTAGct                                                                                     <  1:296539/141‑1 (MQ=255)                                                  aTTCGCTTTCAATCTCGCTGAAAATTGAGTTAATGTCACCCAAAAGTGTTTCTAATTGCAAAATTCCAAATACAGTGTCGTTATTTAC‑TTTTTCATGCTAAACATCTCCCAATTAATAATCACATATTTAGCTACTATACa                                                                             <  2:218181/141‑1 (MQ=255)                                                  aTTCGCTTTCAATCTCGCTGAAAATTGAGTTAATGTCACCCAAAAGTGTTTCTAATTGCAAAATTCCAAATACAGTGTCGTTATTTAC‑TTTTTCATGCTAAACATCTCCCAATTAATAATCACATATTTAGCTACTATACa                                                                             <  1:158513/141‑1 (MQ=255)                                                  aTTCGCTTTCAATCTCGCTGAAAATTGAGTTAATGTCACCCAAAAGTGTTTCTAATTGCAAAATTCCAAATACAGTGTCGTTATTTAC‑TTTTTCATGCTAAACATCTCCCAATTAATAATCACATATTTAGCTACTATACa                                                                             <  2:375961/141‑1 (MQ=255)                                                     cGCTTTCAATCTCGCTGAAAATTGAGTTAATGTCACCCAAAAGTGTTTCTAATTGCAAAATTCCAAATACAGTGTCGTTATTTAC‑TTTTTCATGCTAAACATCTCCCAATTAATAATCACATATTTAGCTACTATACAAGt                                                                          >  2:126426/1‑141 (MQ=255)                                                     cGCTTTCAATCTCGCTGAAAATTGAGTTAATGTCACCCAAAAGTGTTTCTAATTGCAAAATTCCAAATACAGTGTCGTTATTTAC‑TTTTTCATGCTAAACATCTCCCAATTAATAATCACATATTTAGCTACTATACAAGt                                                                          >  2:155049/1‑141 (MQ=255)                                                     cGCTTTCAATCTCGCTGAAAATTGAGTTAATGTCACCCAAAAGTGTTTCTAATTGCAAAATTCCAAATACAGTGTCGTTATTTAC‑TTTTTCATGCTAAACATCTCCCAATTAATAATCACATATTTAGCTACTATACAAGt                                                                          >  1:7072/1‑141 (MQ=255)                                                            aaTCTCGCTGAAAATTGAGTTAATGTCACCCAAAAGTGTTTCTAATTGCAAAATTCCAAATACAGTGTCGTTATTTAC‑TTTTTCATGCTAAACATCTCCCAATTAATAATCACATATTTAGCTACTATACAAGTATTaaca                                                                   >  1:291796/1‑141 (MQ=255)                                                            aaTCTCGCTGAAAATTGAGTTAATGTCACCCAAAAGTGATTCTAATTGCAAAATTCCAAATACAGTGTCGTTATTTAC‑TTTTTCATGCTAAACATCTCCCAATTAATAATCACATATTTAGCTACTATACAAGTATTaaca                                                                   >  1:218705/1‑141 (MQ=255)                                                              tctcGCTGAAAATTGAGTTAATGTCACCCAAAAGTGTTTCTAATTGCAAAATTCCAAATACAGTGTCGTTATTTAC‑TTTTTCATGCTAAACATCTCCCAATTAATAATCACATATTTAGCTACTATACAAGTATTaacaaa                                                                 >  2:356923/1‑141 (MQ=255)                                                                 cGCTGAAAATTGAGTTAATGTCACCCAAAAGTGTTTCTAATTGCAAAATTCCAAATACAGTGTCGTTATTTAC‑TTTTTCATGCTAAACATCTCCCAATTAATAATCACATATTTAGCTACTATACAAGTATTAACAAACAt                                                              >  2:215783/1‑141 (MQ=255)                                                                 cGCTGAAAATTGAGTTAATGTCACCCAAAAGTGTTTCTAATTGCAAAATTCCAAATACAGTGTCGTTATTTAC‑TTTTTCATGCTAAACATCTCCCAATTAATAATCACATATTTAGCTACTATACAAGTATTAACAAACAt                                                              >  2:28927/1‑141 (MQ=255)                                                                 cGCTGAAAATTGAGTTAATGTCACCCAAAAGTGTTTCTAATTGCAAAATTCCAAATACAGTGTCGTTATTTAC‑TTTTTCATGCTAAACATCTCCCAATTAATAATCACATATTTAGCTACTATACAAGTATTAACAAACAt                                                              >  2:8021/1‑141 (MQ=255)                                                                 cGCTGAAAATTGAGTTAATGTCACCCAAAAGTGTTTCTAATTGCAAAATTCCAAATACAGTGTCGTTATTTAC‑TTTTTCATGCTAAACATCTCCCAATTAATAATCACATATTTAGCTACTATACAAGTATTAACAAACAt                                                              >  1:117284/1‑141 (MQ=255)                                                                 cGCTGAAAATTGAGTTAATGTCACCCAAAAGTGTTTCTAATTGCAAAATTCCAAATACAGTGTCGTTATTTAC‑TTTTTCATGCTAAACATCTCCCAATTAATAATCACATATTTAGCTACTATACAAGTATTAACAAACAt                                                              >  1:22937/1‑141 (MQ=255)                                                                  gCTGAAAATTGAGTTAATGTCACCCAAAAGTGTTTCTAATTGCAAAATTCCAAATACAGTGTCGTTATTTAC‑TTTTTCATGCTAAACATCTCCCAATTAATAATCACATATTTAGCTACTATACAAGTATTAACAAACATc                                                             >  2:378217/1‑141 (MQ=255)                                                                               ttAATGTCACCCAAAAGTGTTTCTAATTGCAAAATTCCAAATACAGTGTCGTTATTTAC‑TTTTTCATGCTAAACATCTCCCAATTAATAATCACATATTTAGCTACTATACAAGTATTAACAAACATCCCAACAATCCCaa                                                <  2:27183/141‑1 (MQ=255)                                                                                   tGTCACCCAAACGTGTTTCTAATTGCAAAATTCCAAATACAGTGTCGTTATTTAC‑TTTTTCATGCTAAACATCTCCCAATTAATAATCACATATTTAGCTACTATACAAGTATTAACAAACATCCCAACAATCCCAAAACt                                            <  2:159261/141‑1 (MQ=255)                                                                                      cACCCAAAAGTGTTTCTAATTGCAAAATTCCAAATACCGTGTCGTTATTTAC‑TTTTTCATGCTAAACATCTCCCAATTAATAATCACATATTTAGCTACTATCCAAGTATTAACAAACACCCCAACAATCCCAAAACTTGt                                         >  2:327597/1‑141 (MQ=255)                                                                                      cACCCAAAAGTGTTTCTAATTGCAAAATTCCAAATACAGTGTCGTTATTTAC‑TTTTTCATGCTAAACATCTCCCAATTAATAATCACATATTTAGCTACTATACAAGTATTAACAAACATCCCAACAATCTCAAAACTTGt                                         >  2:307123/1‑141 (MQ=255)                                                                                                   ttCTAATTGCAAAATTCCAAATACAGTGTCGTTATTTAC‑TGTTTCATGCTAAACATCTCCCAATTAATTATCACATATTTAGCTACTATACCAGTATTAACAAACATCCCAACAATCCCACAACTTGTATGTGCTAACTTa                            <  1:402965/141‑1 (MQ=255)                                                                                                         ttGCAAAATTCCAAATACAGTGTCGTTATTTAC‑TTTTTCATGCTAAACATCTCCCAATTAATAATCACATATTTAGCTACTATACAAGTATTAACAAACATCCCAACAATCCCAAAACTTGTATGTGCTAACTTATTCATa                      <  1:72212/141‑1 (MQ=255)                                                                                                         ttGCAAAATTCCAAATACAGTGTCGTTATTTAC‑TTTTTCATGCTAAACATCTCCCAATTAATAATCACATATTTAGCTACTATACAAGTATTAACAAACATCCCAACAATCCCAAAACTTGTATGTGCTAACTTATGCATa                      <  2:231073/141‑1 (MQ=255)                                                                                                           gCAAAATTCCAAATACAGTGTCGTTATTTAC‑TTTTTCATGCTAAACATCTCCCAATTAATAATCACATATTTAGCTACTATACAAGTATTAACAAACATCCCAACAATCCCAAAACTTGTATGTGCTAACTTATGCATaaa                    >  2:155900/1‑141 (MQ=255)                                                                                                                     aaaTACAGTGTCGTTATTTAC‑TTTTTCATGCTAAACATCTCCCAATTAATAATCACATATTTAGCTACTATACAAGTATTAACAAACATCCCAACAATCCCAAAACTTGTATGTGCTAACTTATGCATAAAACTACAAgtg          <  1:8021/141‑1 (MQ=255)                                                                                                                     aaaTACAGTGTCGTTATTTAC‑TTTTTCATGCTAAACATCTCCCAATTAATAATCACATATTTAGCTACTATACAAGTATTAACAAACATCCCAACAATCCCAAAACTTGTATGTGCTAACTTATGCATAAAACTACAAgtg          <  1:28927/141‑1 (MQ=255)                                                                                                                     aaaTACAGTGTCGTTATTTAC‑TTTTTCATGCTAAACATCTCCCAATTAATAATCACATATTTAGCTACTATACAAGTATTAACAAACATCCCAACAATCCCAAAACTTGTATGTGCTAACTTATGCATAAAACTACAAgtg          <  2:22937/141‑1 (MQ=255)                                                                                                                             tgtCGTTATTTAC‑TTTTTCATGCTAAACATCTCCCAATTAATAATCACATATTTAGCTACTATACAAGTATTAACAAACATCCCAACAATCCCAAAACTTGTATGTGCTAACTTATGCATAAAACTACAAGTGtaaataaa  <  1:373073/141‑1 (MQ=255)                                                                                                                                          |                                                                                                                                 TTTTGCCATAAAGTTAGTAAAATTAAAATTTCTTCTCTAGACATTTTGTATTCGCTTTCAATCTCGCTGAAAATTGAGTTAATGTCACCCAAAAGTGTTTCTAATTGCAAAATTCCAAATACAGTGTCGTTATTTACTTTTTTCATGCTAAACATCTCCCAATTAATAATCACATATTTAGCTACTATACAAGTATTAACAAACATCCCAACAATCCCAAAACTTGTATGTGCTAACTTATGCATAAAACTACAAGTGTAAATAAA  >  CP000730/1891762‑1892027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑