| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 1,860,581 | A→G | 39.9% | L1198S (TTG→TCG) | sftA ← | DNA translocase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 1,860,581 | 0 | A | G | 39.9% | 2.4 / 5.6 | 10 | L1198S (TTG→TCG) | sftA | DNA translocase |
| Reads supporting (aligned to +/- strand): ref base A (3/3); new base G (2/2); total (5/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TAATTCTTGCTGCTCTATTATAGCCAATTTGGAAATGTCTTTGGATTAATGATGTTGAAATATGTCCTTCATTAACCATAAATGCACAAACATCATCAAATA‑ATTCATCTTGTGATTGTGTTTGTGTTTTTTTCAACAATTCTTTTTCTTCAAATAGATAGTCCGGTTCTCTTTGTTGTTTGATAAAATCAACAACATCATCAATTTCGTCATCAGAAACAAATGTACCTTGAACTCTAATCGGTTTATTCATACCGCTACCAAGATA > CP000730/1860444‑1860711 | tAATTCTTGCTGCTCTATTATAGCCAATTTGGAAATGTCTTTGGATTAATGATGTTGAAATATGTCCTTCATTAACCATAAATGCACAAACATCATCAAATA‑ATTCATCTTGTGATTGTGTTTGTGTTTTTTTCAACGAtt > 1:62895/1‑141 (MQ=255)tAATTCTTGCTGCTCTATTATAGCCAATTTGGAAATGTCTTTGGATTAATGATGTTGAAATATGTCCTTCATTAACCATAAATGCACAAACATCATCAAATA‑ATTCATCTTGTGATTGTGTTTGTGTTTTTTTCAACGAtt > 1:80219/1‑141 (MQ=255) ttGGAAATGTCTTTGGATTAATGATGTTGAAATATGTCCTTCATTAACCATAAATGCACAAACATCATCAAATA‑ATTCATCTTGTGATTGTGTTTGTGTTTTTTTCAACAATTCTTTTTCTTCAAATAGATAGTCCGGTtc > 2:114023/1‑141 (MQ=255) aTGTCTTTGGATTAATGATGTTGAAATATGTCCTTCATTAACCATACCTGAACAAAAAAC‑CCAGTTACATTTATATTGAGAGTCTTTTTGTGTTTTTTTCAACAATTCTTTTTCTTCAAATAGATAGTCCGGTTCTCTttg < 1:52697/141‑1 (MQ=255) cTTTGGATTAATGATGTTGAAATATGTCCTTCATTAACCATAAATGCACAAACATCATCAAATA‑ATTCATCTTGTGATTGTGTTTGTGTTTTTTTCAACAATTCTTTTTCTTCAAATAGATAGTCCGGTTCTCTTtgttgt > 2:14416/1‑141 (MQ=255) gTTGAAATATGTCCTTCATTAACCATAAATGCACAAACATCATCAAATA‑ATTCATCTTGTGATTGTGTTTGTGTTTTTTTCAACAATTCTTTTTCTTCAAATAGATAGTCCGGTTCTCTTTGTTGTTTGATAAAATcaaca > 1:33461/1‑141 (MQ=255) tCCTTCATTAACCATAAATGCACAAACATCATCAAATA‑ATTCATCTTGTGATTGTGTTTGTGTTTTTTTCAACAATTCTTTTTCTTCAAATAGATAGTCCGGTTCTCTTTGTTGTTTGATAAAATCAACAACATCATCAAt < 2:88633/141‑1 (MQ=255) aCCATAAATGCACAAACATCATCAAATA‑ATTCATCTTGTGATTGTGTTTGTGTTTTTTTCAACAATTCTTTTTCTTCAAATAGATAGTCCGGTTCTCTTTGTTGTTTGATAAAATCAACAACATCATCAATTTCGtcatca < 1:114023/141‑1 (MQ=255) ttttttCAACGATTCTTTTTCTTCAAATAGATAGTCCGGTTCTCTTTGTTGTTTGATAAAATCAACAACATCATCAATTTCGTCATCAGAAACAAATGTACCTTGAACTCTAATCGGTTTATTCATACCGCTACCAAGata < 2:62895/141‑1 (MQ=255) ttttttCAACGATTCTTTTTCTTCAAATAGATAGTCCGGTTCTCTTTGTTGTTTGATAAAATCAACAACATCATCAATTTCGTCATCAGAAACAAATGTACCTTGAACTCTAATCGGTTTATTCATACCGCTACCAAGata < 2:80219/141‑1 (MQ=255) | TAATTCTTGCTGCTCTATTATAGCCAATTTGGAAATGTCTTTGGATTAATGATGTTGAAATATGTCCTTCATTAACCATAAATGCACAAACATCATCAAATA‑ATTCATCTTGTGATTGTGTTTGTGTTTTTTTCAACAATTCTTTTTCTTCAAATAGATAGTCCGGTTCTCTTTGTTGTTTGATAAAATCAACAACATCATCAATTTCGTCATCAGAAACAAATGTACCTTGAACTCTAATCGGTTTATTCATACCGCTACCAAGATA > CP000730/1860444‑1860711 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |