Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	583,939	G→A	6.9%	intergenic (+23/‑249)	rplA → / → rplJ	ribosomal protein L1/ribosomal protein L10

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	583,939	0	G	A	6.9%	215.8 / 2.7	76	intergenic (+23/‑249)	rplA/rplJ	ribosomal protein L1/ribosomal protein L10
Reads supporting (aligned to +/- strand):  ref base G (44/25);  new base A (2/4);  total (46/30)
Fisher's exact test for biased strand distribution p-value = 1.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAGATGTATTAGCTAAAGCTAAACCATCATCTGCTAAAGGTACATACTTCAAATCTGTTGCTGTAACTACAACAATGGGTCCTGGAGTTAAAATT‑GATA‑CTGCAAGTTTCAAATAATAAATGATA‑TA‑AACAATTACAGGCTGAAAGAAATATCTTTCAGTCTGTAAAAATATATTGACAATAAGTAATTTCCAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATT  >  CP000730/583801‑584077                                                                                                                                               |                                                                                                                                           aaGATGTATTAGCTAAAGCTAAACCATCATCTGCTAAAGGTACATACTTCAAATCTGTTGCTGTAACTACAACAATGGGTCCTGGAGTTAAAATT‑GATA‑CTGCAAGTTTCAAATAATAAATGATA‑TA‑AACAATTACAGGc                                                                                                                                           <  2:28503/140‑1 (MQ=255)  aGATGTATTAGCTACAGCTAAACCACCATCTGCGAAAGGTACATACTTCAAATCTGTTGCTGTAACTACAACAATGGGTCCTGGAGTTAAAATT‑GTTA‑CTGCAAGTTTCAAATAATAAATGATA‑TA‑AACAATTACAGGCt                                                                                                                                          <  1:442028/140‑1 (MQ=255)    aTGTATTAGCTAAAGCTAAACCATCATCTGCTAAAGGTACATACTTCAAATCTGTTGCTGTAACTACAACAATGGGTCCTGGAGTTAAAATT‑GATA‑CTGCAAGTTTCAAATAATAAATGATA‑TA‑AACAATTACAGGCTga                                                                                                                                        >  2:629434/1‑140 (MQ=255)        aTTAGCTAAAGCTAAACCATCATCTGCTAAAGGTACATACTTCAAATCTGTTGCTGTAACTACAACAATGGGTCCTGGAGTTAAAATT‑GATA‑CTGCAAGTTTCAAATAATAAATGATA‑TA‑AACAATTACAGACTgaaaga                                                                                                                                    >  1:318308/1‑140 (MQ=255)            gctaaagctaaaCCATCATCTGCTAAAGGTACATACTTCAAATCTGTTGCTGTAACTACAACAATGGGTCCTGGAGTTAAAATT‑GATA‑CTGCAAGTTTCAAATAATAAATGATA‑TA‑AACAATTACAGGCTGAAAGTAata                                                                                                                                <  1:496231/140‑1 (MQ=255)                  gctaaaCCATCATCTGCTAAAGGTACATACTTCAAATCTGTTGCTGTAACTACAACAATGGGTCCTGGAGTTAAAATT‑GATA‑CTGCAAGTTTCAAATAATAAATGATA‑TA‑AACAATTACAGACTGAAAGATAtttctttc                                                                                                                          <  2:318308/140‑1 (MQ=255)                  gctaaaCCATCATCTGCTAAAGGTACATACTTCAAATCTGTTGCTGTAACTACAACAATGGGTCCTGGAGTTAAAATT‑GATA‑CTGCAAGTTTCAAATAATAAATGATA‑TA‑AACAATTACAGACTGAAAGATAtttctttc                                                                                                                          <  1:180777/140‑1 (MQ=255)                   ctaaaACATCATCTACTAAAATTACATACTTCAAATCTGTTGCTGTAACTACAACAATGGGTCCTGGAATTAAAATT‑GATA‑CTGCAAGTTTCAAATAAAACATGATA‑AA‑AACAATTACAGGCTGAAAGAAATATCTTTCa                                                                                                                         <  1:104207/140‑1 (MQ=255)                       tccATCATCTGCTAAAGGTACATACTTCAAATCTGTTGCTGTAACTACAACAATGGGTCCTGGAGTTAAAATT‑GATA‑CTGCAAGTTTCAAATAATAAATGATA‑TA‑AACAATTACAGACTGAAAGATATTTCTTTCAgcct                                                                                                                     <  2:329921/139‑4 (MQ=255)                       aCCATCATCTGCTAAAGGTACATACTTCAAGTCTGTTGCTGGAACTACAACAATGGGTCCTGGAATTAAAATC‑AATA‑AAGCAAGTTTCAAATAATAAATGATA‑TA‑AACAATTACAGGCTGAAAGAAATATCTTTCAGTCt                                                                                                                     <  2:360697/140‑1 (MQ=255)                         catcatCTGCTAAAGGTACATACTTCAAATCTGTTGCTGTAACTGCAACAATGGGTCCTGGAGTTAAAATT‑GATA‑CTGCAAGTTTCAAATAATAAATGATA‑TA‑AACAATTACAGGCTGAAAGAAATATCTTTCAGTCTGt                                                                                                                   >  1:412228/1‑140 (MQ=255)                         catcatCTGCTAAAGGTACATACTTCAAATCTGTTGCTGTAACTACAACAATGGGTCCTGGAGTTAAAATT‑GATA‑CTGCAAGTTTCAAATAATAAATGATA‑TA‑AACAATTACAGGCTGAAAGAAATATCTTTCAGTCTGt                                                                                                                   >  2:426723/1‑140 (MQ=255)                         catcatCTGCTAAAGGTACATACTTCAAATCTGTTGCTGTAACTACAACAATGGGTCCTGGAGTTAAAATT‑GATA‑CTGCAAGTTTCAAATAATAAATGATA‑TA‑AACAATTACAGGCTGAAAGAAATATCTTTAAGTCTGt                                                                                                                   >  2:58143/1‑140 (MQ=255)                         catcatCTGCTAAAGGTACATACTTCAAATCTGTTGCTGTAACTACAACAATGGGTCCTGGAGTTAAAATT‑GATA‑CTGCAAGTTTCAAATAATAAATGATA‑TA‑AAAAATTACAGGCTGAAAGAAATATCTTTCAGTCTGt                                                                                                                   >  1:156510/1‑140 (MQ=255)                           tcatcTGCTAAAGGTACATACTTCAAATCTGTTACTGTAACTACAACAATGGGTCCTGGAGTTAGAATT‑GATA‑CTGCAAGTTTCAAATAATAAATGATA‑TA‑AACAATTACAGGCTGAAAGAAATATCTTTCAGTCTGTaa                                                                                                                 >  2:164806/1‑140 (MQ=255)                              tcTGCTAAAGGTACATACTTCAAATCTGTTGCTGTAACTACAACAATGGGTCCTGGAGTTAAAATT‑GATA‑CTGCAAGTTTCAAATAATAAATGATA‑TA‑AACAATTACAGGCTGAAAGAAATATCTTTCAGTCTGTaaaaa                                                                                                              <  2:547740/140‑1 (MQ=255)                              tcTGCTAAAGGTACATACTTCAAATCTGCTGCTGGAACTACAACGATGAGGCCTGACGTTAAAATT‑GATA‑CTGCAAGTTTCAAGTAATAAATGGAA‑TA‑AATAATTACAGGATCGAAGAAATATTGTTCAGTCtcgcacca                                                                                                              >  2:177105/1‑133 (MQ=255)                              tcTGCTAAAGGTACATACTTCAAATCTGATGCTGCACAAAAAACAAAGGTTTCTGGAGTTAAAATT‑GATA‑CTGCAAGTTTCAAATAATAAGTGATA‑TA‑AACAATTACAGGCTGAAAGCAAGAACTTTAAGTCTGtaataa                                                                                                              >  2:119480/1‑140 (MQ=255)                              tcTGCTAAAGCTACATACTTCAAATCTGTCGCTGTAACTACAACAAGGGATGCTCGTGTTAAAATT‑TATA‑CTGCAAGTTTACAATAATAAATGATA‑TA‑CACACTTTCAGGCTGAAAGAAATATCTTTCATTCTATaaaaa                                                                                                              >  2:177050/1‑140 (MQ=255)                               cTGCTAAAGGTACATACTTCAAATCTGTTGCTGTAACTACAACAATGGGTCCTGGAGTTAAAATT‑GATA‑CTGCAAGTTTCAAATAATAAATGATA‑TA‑AACAATTACAGGCTGAAAGAAATATCTTTCAGTCTGTAAAAat                                                                                                             >  1:162693/1‑140 (MQ=255)                                tacaaaaGGAACAGACTTCAAAACTGTTGCTCTAACTACAACAATGGATCCTGCAATTAAAATA‑AAAA‑CTGCAAGTTTCAAATAATAAATGATA‑TA‑AACAATCACAGACTAAAAGAAATATCTTTCAGTCTTTAAAAata                                                                                                            <  2:195428/136‑1 (MQ=255)                                tGCTAAAGGTACATACTTCAAATCTGTTGCTGTAACTACAACAATGGGTCCTGGAGTTAAAATT‑GATA‑CTGCAAGTTTCAAATAATAAATGATA‑TA‑AACAATTACAGGCTGAAAGAAATATCTTTCAGTCTGTAAAAata                                                                                                            >  1:32535/1‑140 (MQ=255)                                 gCTAAAGGTACATACTTCAAATCTGTTGCTGTAACTACAACAATGGGTCCTGGAGTTAAAATT‑GATA‑CTGCAAGTTTCAAATAATAAATGATA‑TA‑AACAATTACAGGCTGAAAGAAATATCTTTCAGTCTGTAAAAatat                                                                                                           <  2:702800/140‑1 (MQ=255)                                     aaGGTACATACTTCAAATCTGTTGCTGTAACTACAACAATGGGTCCTGGAGTTAAAATT‑GATA‑CTGCAAGTTTCAAATAATAAATGATA‑TA‑AACAATTACAGGCTGAAAGAAATATCTTTCAGTCTGTAAAAATATATTg                                                                                                       <  2:191682/140‑1 (MQ=255)                                       ggTACATACTTCAAATCTGTTGCTGTAACTACAACAATGGGTCCTGGAGTTAAAATT‑GATA‑CTGCAAGTTTCAAATAATAAATGATA‑TA‑AACAATTACAGGCTGAAAGAAATATCTTTCAGTCTGTAAAAATATATTGAc                                                                                                     >  1:65661/1‑140 (MQ=255)                                         tACATACTTCAAATCTGTTGCTGTAACTACAACAATGGGTCCTGGAGTTAAAATT‑GATA‑CTGCAAGTTTCAAATAATAAATGATA‑TA‑AACAATTACAGGCTGAAAGAAATATCTTTCAGTCTGTAAAAATATATTGACaa                                                                                                   >  2:680260/1‑140 (MQ=255)                                            aTACTTCAAATCTGTTGCTGTAACTACAACAATGGGTCCTGGAGTTAAAATT‑GATA‑CTGCAAGTTTCAAATAATAAATGATA‑TA‑AACAATTACAGGCTGAAAGAAATATCTTTCAGTCTGTAAAAATATATTGACAATaa                                                                                                >  1:696236/1‑140 (MQ=255)                                            aTACTTCAAACCTGTTGCTGTAACTACAACAATGGGTCCTGGAGTTAAAATT‑GATA‑CTGCAAGTTTCAAATAATAAATGATA‑TA‑AACAATTACAGGCTGAAAGAAATATCTTTCAGTCTGTAAAAATATATTGACAATaa                                                                                                <  2:213845/140‑1 (MQ=255)                                              aCTTCAAATCTGTTGCTGTAACTACAACAATGGGTCCTGGAGTTAAAATT‑GATA‑CTGCAAGTTTCAAATAATAAATGATA‑TA‑AACAATTACAGGCTGAAAGAAATATCTTTCAGTCTGTAAAAATATATTGACAATAAGt                                                                                              >  1:359203/1‑140 (MQ=255)                                              aCTTCAAATCTGTTGCTGTAACTACAACAATGGGTCCTGGAGTTAAAATT‑GATA‑CTGCAAGTTTCAAATAATAAATGATA‑TA‑AACAATTACAGGCTGAAAGAAATATCTTTCAGTCTGTAAAAATATATTGACAATAAGt                                                                                              >  1:467530/1‑140 (MQ=255)                                               cTTCAAATCTGTTGCGGTAACTACAACAATGGGTCCTGGAGTTAAAATT‑GATA‑CTGCAAGTTTCAAATAATAAATGATA‑TA‑AACGATGACAGACTGAAAGAAATATCTTTCAGTCTGTAAAAATATATTGACAATAAGTa                                                                                             >  1:536694/1‑140 (MQ=255)                                                  cAAATCTGTTGCTGTAACTACAACAATGGGTCCTGGAGTTAAAATT‑GATA‑CTGCAAGTTTCAAATAATAAATGATA‑TA‑AACAATTACAGGCTGAAAGAAATATCTTTCAGTCTGTAAAAATATATTGACAATAAGTAAtt                                                                                          <  1:358143/140‑1 (MQ=255)                                                     aTCTGTTGCTGTAACTACAACAATGGGTCCTGGAGTTAAAATT‑GATA‑CTGCAAGTTTCAAATAATAAATGATA‑TA‑AACAATTACAGGCTGAAAGAAATATCTTTCAGTCTGTAAAAATATATTGACAATAAGTAATTTcc                                                                                       <  2:614312/140‑1 (MQ=255)                                                     aTCGGTTGCTGTAACTACAACAAGGGGTCCTGGAGTTAAAGTT‑GTTATCTGC‑AGTTGCAAATAATAACTGATA‑TA‑AACAATTACAGGCTGAAAGAAAGATCTTTCAGGCTGTAAAAATATATTGACAATAAGTAATTTcc                                                                                       <  1:137121/140‑1 (MQ=255)                                                        tGTTGCTGTAACTACAACAATGGGTCCTGGAGTTAAAATT‑GATA‑CTGCAAGTTTCAAATAATAAATGATA‑TA‑AACAATTACAGGCTGAAAGAAATATCTTTCAGTCTGTAAAAATATATTGAAAATAAGTAATTTCCAAg                                                                                    >  2:521360/1‑140 (MQ=255)                                                         gTTGCTGTAACTACAACAATGGGTCCTGGAGTTAAAATT‑GATA‑CTGCAAGTTTCAAATAATAAATGATA‑TA‑AACAATTACAGGCTGAAAGAAATATCTTTCAGTCTGTAAAAAAATATTGACAATAAGTAATTTCCAGGt                                                                                   >  2:554370/1‑140 (MQ=255)                                                         gTTGCTGTAACTACAACAATGGGTCCTGGAGGTAAAATT‑GATA‑CTGCAAGTTTCAAATAATAAATGATA‑TA‑AACAATTACAGGCTGAAAGAAATATCTTTCAGTCTGTAAAAATATATTGACAATAAGTAATTTCCAAGt                                                                                   >  2:558717/1‑140 (MQ=255)                                                         gTTGCTATAATTAAAACAATGGGTCCTGGAGTTAAAATT‑GATA‑CTGCAAGTTTCAAATAATAAATGATA‑TA‑AACAATTACAGGCTGAAAGAAATATCTTTCAGTCTGTAAAAATATATTGACAATAAGTAATTTCCAAGt                                                                                   <  1:318469/140‑1 (MQ=255)                                                          ttGCTGTAACTACAACAATGGGTCCTGGAGTTAAAATT‑GATA‑CTGCAAGTTTCAAATAATAAATGATA‑TA‑AACAATTACAGGCTGAAAGAAATATCTTTCAGTCTGTAAAAATATATTGACAATAAGTAATTTCCAAGtt                                                                                  >  2:215464/1‑140 (MQ=255)                                                            gCTGTAACTACAACAATGGGTCCTGGAGTTAAAATT‑GATA‑CTGCAAGTTTCAAATAATAAATGATA‑TA‑AACAATTACAGGCTGAAAGAAATAGCTTTCAGTCTGTAAAACTATATTGACAATAAGTAATTTCCAGGtttt                                                                                >  1:117935/1‑138 (MQ=255)                                                              tGTAACTACAACAATGGGTCCTGGAGTTAAAATT‑GATA‑CTGCAAGTTTCAAATAATAAATGATA‑TA‑AACAATTACAGGCTGAAAGAAATATCTTTCAGTCTGTAAAAATATATTGACAATAAGTAATTTCCAAGTTatat                                                                              <  2:32535/140‑1 (MQ=255)                                                              cgTAACTAAAACAATGGGTCCTGGAGTTAAAATT‑GATA‑CTGCAAGTTTCAAATAATAAATGATA‑TA‑AACAATTACAGGCTGAAAGAAATATCTTTCAGTCTGTAAAAATATATTGACAATAAGTAATTTCCAAGTTatat                                                                              <  2:316094/139‑1 (MQ=255)                                                                  acgaaaacaaTGTGTCCGGGAGTTAAAATT‑GATA‑CTGCAAGTTTCAAATAATAAATGATA‑TA‑AACAATTACAGGCTGAAAGAAATATCTTTCAGTCTGTAAAAATATATTGACAATAAGTAATTTCCAAGTTATATTACt                                                                          <  2:259248/135‑1 (MQ=255)                                                                       aacaaTGGGTCCTGGAGTTAAAATT‑GATA‑CTGCAAGTTTTAAATAATAAATGATA‑TA‑AACAATTACAGGCTGAAAGAAATATCTTTCAGTCTGTAAAAATATATTGACAATAAGTAATTTCCAAGTTATATTACTTATtg                                                                     <  2:685119/140‑1 (MQ=255)                                                                            tGGGTCCTGGAGTTAAAATT‑GATA‑CTGCAAGTTTCAAATAATAAATGATC‑TA‑AACAATTACAGGCTGAAAGAAATATCTTTCAGTCTGTAAAAATATATTGACAATAAGTAATTTCCAAGTTATATTACTTATTGTGatt                                                                >  2:122179/1‑140 (MQ=255)                                                                                tCCTGGAGTTAAAATT‑GATA‑CTGCAAGTTTCAAATAATAAATGATA‑TA‑AACAATTACAGGCTGAAAGAAATATCTTTCAGTCTGTAAAAATATATTGACAATAAGTAATTTCCAAGTTATATTACTTATTGTGATTAttt                                                            >  2:649539/1‑140 (MQ=255)                                                                                  cTGGAGTTAAAATT‑GATA‑CTGCAAGTTTCAAATAATAAATGATA‑TA‑AACAATTACAGGCTGAAAGAAATATCTTTCAGTCTGTAAAAATATATTGGCAATAAGTAATTTCCAAGTTATTTTGTTTATAGTGATTGTTTTa                                                          >  1:291599/1‑140 (MQ=255)                                                                                   tGGAGTTAAAATT‑GATA‑CTGCAAGTTTCAAATAATAAATGATA‑TACCATCATTA‑GGTCTAAAAGAAATATCTTTCAGTCTGTAAAAATATATTGACAATAAGTAATTTCCAAGTTATATTACTTATTGTGATTATTTTAc                                                         <  1:680260/140‑1 (MQ=255)                                                                                    ggAGTTAAAATT‑GATA‑ATTCAAGGTTCAAATAAAAAATGATA‑TA‑AACAATTACAGGCTGAAAGAAATATCTTTCAGTCTGTAAAAATATATTGACAATAAGTAATTTCCAAGTTATATTACTTATTGTGATTATTTTAcc                                                        <  1:55312/140‑1 (MQ=255)                                                                                       gTTAAAATT‑GATA‑CTGTAAGTTTCAAATAATAAATGATA‑TA‑AACAATTACAGGCTGAAAGAAATATCTTTCAGTCTGTAAAAATATATTGACAATAAGTAATTTCCAAGTTATATTACTTATTGTGATTATTTTACCTaa                                                     <  1:673881/140‑1 (MQ=255)                                                                                         tAAAATT‑GATA‑CTGCAAGTTTCAAATAATAAATGATA‑TA‑AACAATTACAGGCTGAAAGAAATATCTTTCAGTCTGTAAAAATATATTGACAATAAGTAATTTCCAAGTTAGATTACTTATTGTGATTATTTTACCTAAGa                                                   <  1:451042/140‑1 (MQ=255)                                                                                           aaaTTGGATA‑CTGCAAGTTTCAAATTATAAATGATATTA‑ACCAATTA‑TTGCTGAAAGAAATATCCTTCAGTCTGTAAAAATATATTGACAATAAGTAATTTCCAAGTTATATTACTTATTGTGATTATTTTACCTAAGAc                                                  <  2:116024/140‑1 (MQ=255)                                                                                                 gATA‑CTGCAAGTTTCAAATAATAAATGATA‑TA‑AACAATTACAGGCTGAAAGAAATATCTTTCAGTCTGTAAAAATATATTGACAATAAGTAATTTCCAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAgg                                            >  2:489114/1‑140 (MQ=255)                                                                                                    a‑CTGCAAGTTTCAAATAATAAATGATA‑TA‑AACTATTACAGGCTGAAAGAAATATCTTTCAGTCTGTAAAAATATATTGACAATAAGTAATTTCCAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGt                                         <  1:281188/140‑1 (MQ=255)                                                                                                        gCATGTTTCAAATAATAAATGATA‑TA‑AACAATTACAGGCTGAAAGAAATATCTTTCAGTCTATAAAAATATATTGACAATAAGTAATTTCCAAGTTATATTACTTATTGTGATTAGTTTACTTACGAccatagatgttct                                      >  2:7546/1‑128 (MQ=255)                                                                                                        gCAAGTTTCAAATAATAAATGATA‑TA‑AACAAATACAGGCTGAAAGAAATATCTTTCATTCTGTAAAAATATATTGACAATAAGTAATTTCCAAGTTATATTACATATTGTGATTATTTTACCTAAGACAGTAGGAGttat                                      >  2:270670/1‑140 (MQ=255)                                                                                                          aaGTTTCAAATAATAAATGATA‑TA‑AACAATTACAGGCTGAAAGAAATATCTTTCAGTCTGTAAAAATATATTGACAATAAGTAATTTCCAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGttattt                                    >  1:700112/1‑140 (MQ=255)                                                                                                          aaGTTTCAAATAATAAATGATA‑TA‑AACAATTACAGGCTGAAAGAAATATCTTTCAGTCTGTAAAAATATATTGACAATAAGTAATTTCCAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGttattt                                    >  1:653280/1‑140 (MQ=255)                                                                                                           aGTTTCAAATAATAAATGATA‑TA‑AACAATTACAGGCTGAAAGAAATATCTTTCCGTATGTAAAAATATATTGACAATAAGTAATTTCCAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAATAGGAGttattta                                   >  1:530808/1‑140 (MQ=255)                                                                                                           aGTTTCAAATAATAAATGATA‑TA‑AACAATTACAGGCTGAAAGAAATATCTTTCAGTCTGTAAAAATATATTGACAATAAGTAATTTCCAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGttattta                                   >  1:651845/1‑140 (MQ=255)                                                                                                           aGTTTCAAATAATAAATGATA‑TA‑AACAATTACAGGCTGAAAGAAATATCTTTCAGTCTGTAAAAATATATTGACAATAAGTAATTTCCAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGttattta                                   >  2:597506/1‑140 (MQ=255)                                                                                                           aGTTTCAAATAATAAATGATA‑TA‑AACAATTACAGGCTGAAAGAAATATCTTTCAGTCTGTAAAAATATATTGACAATAAGTAATTTCCAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGttattta                                   >  2:597512/1‑140 (MQ=255)                                                                                                            gTTTCAAATAATAAATGATT‑TA‑AGCAATTACAGGCTTAGGGAGATATCTTTCAGTCTGTAAAAATATATTGACAATGAGTAATTTCCAAGTTATATTGCTTATTGTGATTATTTTATCTTAGACAGTAGGAGttatttgg                                  >  2:376894/1‑138 (MQ=255)                                                                                                            gTTTCAAATAATAAATGATA‑TA‑AACAATTACAGGCTGAAAGAAATATCTTTCAGTCTGTAAAAATATATTGACAATAAGTAATTTCCAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGttatttat                                  >  2:48181/1‑140 (MQ=255)                                                                                                            gTTTCAAATAATAAATGATA‑TA‑AACAATTACAGGCTGAAAGAAATATCTTTCAGTCTGTAAAAATATATTGACAATAAGTAATTTCCAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGttatttat                                  <  1:629434/140‑1 (MQ=255)                                                                                                                cAAATAATAAATGATA‑TA‑AACAATTACAGGCTGAAAGAAATATCTTTCAGTCTGTAAAAATATATTGACAATAAGTAATTTCCAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACt                              >  2:170183/1‑140 (MQ=255)                                                                                                                 aaaTAATAAATGATA‑TA‑AACAATTACAGGCTGAAAGAAATATCTTTCAGTCTGTAAAAATATATTGACAATAAGTAATTTCCAAGTTATATTACTTATTGTGATTATTTTACCTACGACATTAGGAGTTATTTATAACtt                             <  2:156510/140‑1 (MQ=255)                                                                                                                  aataatAAATGATA‑TA‑AACAATTACGGGCTGAAAGAAATATCTTTCAGTCTGTAAAAATATATTGACAATAAGTAATTTCAAAGTTATTTTACTTATTGTAATTATTTTAACTAAAAAAGTAGGAAGTATTTATAACTTa                            >  1:30490/1‑140 (MQ=255)                                                                                                                  aataatAAATGATA‑TA‑AACAATTACAGGCTGAAAGAAATATCTTTCAGTCTGTAAAAATATATTGACAATAAGTAATTTCCAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTa                            >  2:348252/1‑140 (MQ=255)                                                                                                                      ataaATGATA‑TA‑AACAATTACAGGCTGAAAGAAATATCTTTCAGTCTGTAAAAATATATTGACAATAAGTAATTTCCAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAAt                        <  1:164806/140‑1 (MQ=255)                                                                                                                          aTGATA‑TA‑AACAATTACAGGCTGAAAGAAATATCTTTCAGTCTGTAAAAATATATTGACAATAAGTAATTTCCAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTAt                    >  1:664721/1‑140 (MQ=255)                                                                                                                             ata‑tA‑AACAATTACAGGCTGAAAGAAATATCTTTCAGTCTGTAAAAATATATTGACAATAAGTAATTTCCAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCt                 >  2:474063/1‑140 (MQ=255)                                                                                                                                    aaCAATTACAGGCTGAAAGAAATATCTTTCAGTCTGTAAAAATATATTGACAATAAGTAATTTCCAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGa            >  1:363812/1‑140 (MQ=255)                                                                                                                                    aaCAATTACAGGCTGAAAGAAATATCTTTCAGTCTGTAAAAATATATTGACAATAAGTAATTTCCAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGa            >  1:360407/1‑140 (MQ=255)                                                                                                                                          tACAGGCTGAAAGAAATATCTTTCAGTCTGTAAAAATATATTGACAATAAGTAATTTCCAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCGaa      >  2:520136/1‑140 (MQ=255)                                                                                                                                           aacggCTGAAAGAAATATCTTTCAGTCTGTAAAAATATATTGACAATAAGTAATTTCCAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCAAGGCTaaa     >  2:628934/4‑140 (MQ=255)                                                                                                                                            cAGGCTGAAAGAAATATCTTTCAGTCTGTAAAAATATATTGACAATAAGTAATTTCCAAGTTATAGTACGTATTGTGATTATTTTACCTACGACAGTAGGCGTTATGTATAACTTCAAATTTATCCTGCCGAGGCTcaaa    >  2:370589/1‑140 (MQ=255)                                                                                                                                              ggCTGAAAGAAATATCTTTCAGTCTGTAAAAATATATTGACAATAAGTAATTTCCAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATATATAAATTAAAATTTATCCTGCCAAGGCTAAAAtt  >  1:647334/1‑140 (MQ=255)                                                                                                                                               |                                                                                                                                           AAGATGTATTAGCTAAAGCTAAACCATCATCTGCTAAAGGTACATACTTCAAATCTGTTGCTGTAACTACAACAATGGGTCCTGGAGTTAAAATT‑GATA‑CTGCAAGTTTCAAATAATAAATGATA‑TA‑AACAATTACAGGCTGAAAGAAATATCTTTCAGTCTGTAAAAATATATTGACAATAAGTAATTTCCAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATT  >  CP000730/583801‑584077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑