| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 479,553 | G→A | 100% | A194T (GCA→ACA) | USA300HOU_0458 → | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 479,553 | 0 | G | A | 100.0% | 88.6 / ‑6.5 | 29 | A194T (GCA→ACA) | USA300HOU_0458 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (22/6); minor base C (1/0); total (23/6) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TGATGCAATAATAGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATGCATGGTTGCATTTAGCG‑CAACATGACCATAG‑TTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAG > CP000730/479417‑479673 | tGATGCAATAATAGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACcatacat < 1:55756/140‑1 (MQ=255) tGCAATAATAGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGt < 1:134292/140‑1 (MQ=255) tGCAATAATAGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAACGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGt < 1:74532/140‑1 (MQ=255) aataGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCAtt > 2:56907/1‑140 (MQ=255) aGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAg > 1:54577/1‑140 (MQ=255) gATGAACAAGTTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAgc < 1:18908/140‑1 (MQ=255) ggTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAGTTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCG‑CAACATGa > 2:10026/1‑140 (MQ=255) aGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGACACTTTATATCGATCAATTTCTATCTAGTTGGACAATGCCGAAGCGTGAGCAACGTTTTTACCATACATGGTTGCATTTAACG‑CAACATGACCAt > 1:188807/1‑140 (MQ=255) cACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCG‑CAACATGACCATAG‑t > 1:78032/1‑140 (MQ=255) cTTAGCGAACAAGTGACTCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCG‑CAACATGACCATAG‑ttt > 1:302539/1‑140 (MQ=255) cTTAGCGAACAAGTGAATCGTCAAATGATTAACTGGACGAAACTTTATATCGCTCAATTCCTATAGAGTTGCACAATGCGGAAGAACTACCTATGGTTTTACCATACATGGTTGCGTTTAGCG‑CAACATGACCATAG‑ttt > 1:60827/1‑140 (MQ=255) cTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCG‑CAACATGACCATAG‑ttt > 1:310975/1‑140 (MQ=255) agcGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCG‑CAACATGACCATAG‑TTTTACTAAAGCACAgcgc < 1:92601/138‑1 (MQ=255) aTCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCG‑CAACATGACCATAG‑TTTTACTAAAGCACAgcgc > 1:173419/1‑140 (MQ=255) ttAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCG‑CAACATGACCATAG‑TTTTACTAAAGCACAGCGCCAAGTGATTaaa < 1:15380/140‑1 (MQ=255) aTGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCG‑CAACATGACCATAG‑TTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCt > 2:250440/1‑140 (MQ=255) gACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCG‑CAACATGACCATAG‑TTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTAc > 1:187086/1‑140 (MQ=255) aaCTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCG‑CAACATGCCCATAG‑TTTTACTAAAGCACAGCGCCAGATGATTAAAGGCTTACCCAAt > 2:70683/1‑140 (MQ=255) tttATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATGTAGCG‑CAACATGACCATAG‑TTTTACTAGAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGAt > 2:196924/1‑140 (MQ=255) tatCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCG‑CAACATGACCATAG‑TTTTACTAAAGCACAGCGCCAAGTGATGAAAGGCTTACCCAATGATCCTg > 2:171761/1‑140 (MQ=255) tCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCG‑CAACATGACCATAG‑TTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTCCCCAATGATCCTGaa > 2:8732/1‑140 (MQ=255) tCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCG‑CAACATGACCATAG‑TTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGaa > 2:77038/1‑140 (MQ=255) tttCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATCGATGTGCACATGTGGCGTCAGGAT‑TCC‑TAGTTTTTACTAAAGCACAGCGCCAAGTGACTAAATGCTTACCCAGTGATCCTGAACTGGCGAt > 1:153791/1‑140 (MQ=255) tttCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCG‑CAACATGACCGTAG‑CCCTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGACCCTGAAATGACGAt > 1:74026/1‑140 (MQ=255) tttCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCG‑CAACATGACCATAG‑TTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGAt > 1:259974/1‑140 (MQ=255) tttCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCG‑CAACATGACCATAG‑TTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGAt > 1:138658/1‑140 (MQ=255) tttCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCG‑CAACATGACCATAG‑TTTTACTAAAGCACAGCGCCAAGTGATTAAAAGATTACCCAATGAAACTGAAATGACGAt > 1:96937/1‑140 (MQ=255) gAAGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCG‑AAACATGACCATAG‑TTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCAttttt < 1:10026/140‑1 (MQ=255) aaGCGTGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCG‑CAACATGACCATAG‑TTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCACTGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTc > 2:206136/1‑140 (MQ=255) tGAGCAAAGTTTTTACCATACATGGTTGCATTTAGCG‑CAACATGACCATAG‑TTTTACTAAAGCCCAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGACATGCCGATGGCGTCAGTATTAACTCATTTTTCCAtcg > 1:224250/1‑138 (MQ=255) | TGATGCAATAATAGATGAACAAGGTGAGCCACTTAGCGAACAAGTGAATCGTCAAATGATTAAATGGACGAAACTTTATATCGATCAATTTCTATCGAGTTGGACAATGCCGAAGCGTGAGCAAAGTTTTTACCATGCATGGTTGCATTTAGCG‑CAACATGACCATAG‑TTTTACTAAAGCACAGCGCCAAGTGATTAAAGGCTTACCCAATGATCCTGAAATGACGATAGAGTCAGTATTAACTCATTTTTCAATAG > CP000730/479417‑479673 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |