BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	35,997	T→C	30.9%	T185T (ACA→ACG)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	35,997	0	T	C	30.9%	365.1 / 259.6	326	T185T (ACA→ACG)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base T (118/105); new base C (42/58); total (161/165)
Fisher's exact test for biased strand distribution p-value = 7.25e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.70e-01

Only 100 of 326 total aligned reads displayed.

AGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTT‑TCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACC  >  CP000730/35868‑36135

aGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACt                                                                                                                                  >  2:2305/1‑141 (MQ=14)

 gtgtTCGTTTAACTTGCTAGCATGATGCTAATTTTGTGGTATGGTGAAAATCCGTATATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACtt                                                                                                                                 >  2:279205/1‑141 (MQ=11)

 gtgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACtt                                                                                                                                 <  2:165168/141‑1 (MQ=12)

  tgtTCGTCTAACTTGCTAGCATGATGCTAATTTCGGGGCATGGGTAAACTCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTGTTTGCCGTATTGATACttt                                                                                                                                >  2:235536/1‑141 (MQ=11)

   gtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTg                                                                                                                               >  1:91551/1‑141 (MQ=17)

   gtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTg                                                                                                                               >  1:218223/1‑141 (MQ=17)

     tcgtttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGAt                                                                                                                             >  2:289801/5‑141 (MQ=12)

     tCGTTTAACGTGCTAGCATGATGCTAATTGCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGAt                                                                                                                             <  1:254666/141‑1 (MQ=255)

     tCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGAt                                                                                                                             >  1:34949/1‑141 (MQ=32)

       gCTTGACTGGCTAACATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCGGGCGAGACGAGGGGTTCTTTTTAGATATGGCGTAAATACACTCCATACCTTATAAAGTATTCCTTGCTATCTTGATAcgtggagac                                                                                                                           >  2:18553/1‑133 (MQ=2)

        tttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATatc                                                                                                                          >  1:108439/2‑139 (MQ=11)

         ttAACTTGCTAGCATGATGCTAAGTTCTTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCt                                                                                                                         <  2:260539/141‑1 (MQ=11)

          tAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCtt                                                                                                                        >  1:240228/1‑141 (MQ=255)

           aaCTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTg                                                                                                                       >  2:123543/1‑141 (MQ=32)

             cTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtc                                                                                                                     >  1:278805/1‑141 (MQ=17)

              ttGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtct                                                                                                                    <  1:196818/141‑1 (MQ=17)

               tGCTTGCATGATGCTAATTTCGTGTCATGGCAAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctt                                                                                                                   >  2:331188/1‑141 (MQ=255)

               tGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctt                                                                                                                   <  2:257462/141‑1 (MQ=17)

                 cTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtctt‑tc                                                                                                                >  1:132981/1‑141 (MQ=255)

                 cTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctt‑tc                                                                                                                <  1:72235/141‑1 (MQ=17)

                        gatgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTT‑TCTTACttt                                                                                                         <  2:265513/141‑1 (MQ=25)

                             taagtgcGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAGTGTCTTTTAAAGTCTTCTTTGCTGTCTTTATACTTAGATACTTTGTCTT‑TCTTACTGTCatat                                                                                                    >  2:168161/7‑141 (MQ=11)

                             tAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTT‑TCTTACTTTAatat                                                                                                    >  2:52750/1‑141 (MQ=25)

                             tAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTT‑TCTTACTTTAatat                                                                                                    >  2:100829/1‑141 (MQ=21)

                              aaTTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGCGACCTGCAGTTCTTTTTATATAGAGCGTTAATACCGCCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTT‑TCTTACTTTAATATg                                                                                                   <  1:159455/141‑1 (MQ=11)

                                tttCGTGACATGGCGAAAATCCGGAGATCCCAAGAGACCTGCGGATATTTTTATATAGAGCGTAAATACACCCAATACCTTTTACAGTAGTCTGAACTGAATGGATACCTTACTACCTCGTCTT‑TCTCACttaaccatgcc                                                                                                 >  2:47485/1‑132 (MQ=2)

                                 ttCGTGGCATGGGGAAAATCCGTAGATCTGAATAGACCTACGGTTCTTTTTATCTATAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTTATAATATCAAATCTTGTCTT‑ACTTCCTTTacactgccc                                                                                                >  2:91279/1‑133 (MQ=11)

                                  tCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTT‑TCTTACTTTAATATGACgg                                                                                               <  2:299381/141‑1 (MQ=37)

                                   cGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTTAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTT‑TCTTACTTTAATATGACGGt                                                                                              <  1:171511/141‑1 (MQ=255)

                                      ggCATGGGGAAAATCCGTAGATCTGAAGAGCCCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTT‑TCTTACTTTAATATGACGGttag                                                                                           >  2:203015/1‑138 (MQ=11)

                                      ggCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCCATCCATTTTAAAGTTGTCGTTGCTGTATTGATACTTTGATACCTTGTCTT‑TCTTACTTTAATATGACGGTggt                                                                                           >  2:118692/1‑139 (MQ=11)

                                        cATGGCGAAAACTCGTAGATCTGAAGAGACCCGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTT‑TCTTACTTTAATATGACGGTGATCt                                                                                         <  1:119867/141‑1 (MQ=255)

                                          tGGGGAAAATCCGTAAATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTT‑TCTTACTTTATTATGACGGTGATCTTg                                                                                       <  1:165294/141‑1 (MQ=14)

                                                aaaTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTT‑TCTTACTTTAATATGACGGTGATTTTGCTCAAt                                                                                 >  2:126221/1‑141 (MQ=255)

                                                aaaTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTATGCTGTATTGATACTTTGATACCTTGTCTT‑TCTTACTTTAATATGACGGTGATCTTCGTCAAt                                                                                 >  1:224549/1‑141 (MQ=255)

                                                 aaTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTT‑TCTTACTTTAATATGACGGTGATCTTGCTCAATg                                                                                <  2:65858/141‑1 (MQ=38)

                                                  aTCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTT‑TCTTACTTTAATATGACGGTGATCTTGCTCAATGa                                                                               <  1:137689/141‑1 (MQ=255)

                                                   tCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTT‑TCTTACTTTAATATGACGGTGATCTTGCTCAATGAg                                                                              <  1:5660/141‑1 (MQ=38)

                                                    ccGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTT‑TCTTACTTTAATATGACGGTGATCTTGCTCAATGAgg                                                                             >  1:235827/1‑141 (MQ=39)

                                                    ccGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTT‑TCTTACTTTAATATGACGGTGATATGGCTCAATGAgg                                                                             >  1:147573/1‑141 (MQ=255)

                                                    ccGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTT‑TCTTACTTTAATATGACGGTGATCTTGccacatacgg                                                                             >  2:21540/1‑132 (MQ=255)

                                                    ccGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTT‑TCTTACTTTAATATGACGGTGATCTTGCTCAATGAgg                                                                             <  2:247187/141‑1 (MQ=255)

                                                     cGTAGATCTGAAGGGGACTACGCGTCTTTTTATATAGAGCGTAAATACATTTAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTT‑TCTTACTTTAATATGACGGTGATCTAGCTCGATGAGGt                                                                            <  2:39438/141‑1 (MQ=11)

                                                     cGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTT‑TCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGt                                                                            >  1:324879/1‑141 (MQ=38)

                                                     cGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTT‑TCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGt                                                                            >  1:111078/1‑141 (MQ=255)

                                                         gATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTT‑TCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTAtt                                                                        >  1:321413/1‑141 (MQ=255)

                                                          aTCTGACGCGACCTGCGCTCCTTTTTATATAGACCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTT‑TCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTc                                                                       <  1:189798/141‑1 (MQ=14)

                                                          aTCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTT‑TCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTc                                                                       >  1:43951/1‑141 (MQ=38)

                                                                 gagaCCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTT‑TCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATAtt                                                                <  2:103487/141‑1 (MQ=255)

                                                                  agaCCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTT‑TCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATAttt                                                               <  2:150899/141‑1 (MQ=255)

                                                                   gaCCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTATAAAGTATTCTATGCTGTATTGATACTTTGATACCTTGTCTT‑TCTTACTTTAATATGACGGTGATCTTGCTCACTGAGGTTATTCAGATGTTTc                                                              >  2:62123/1‑141 (MQ=11)

                                                                    aCCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGAATTATGTTGC‑TGAAAATGACGGTGATCTTGCTCAATGCGGTTATTCAGATATTTCg                                                             >  1:326883/1‑141 (MQ=11)

                                                                     ccTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTT‑TCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGa                                                            >  1:256429/1‑141 (MQ=38)

                                                                        tcGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTT‑TCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGt                                                         <  2:240265/140‑1 (MQ=255)

                                                                        gCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTTTTGTTTGCTGTATTGATACTTTGATACCTTGTCTT‑TCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGt                                                         <  1:75815/141‑1 (MQ=255)

                                                                          ggTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTT‑TCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTAc                                                       >  2:254890/1‑141 (MQ=38)

                                                                             tCTTTTTATATAGAGCGTAAATACACTCAACACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTT‑TCTTACTTTAATATGACGGTGATCTTGCTAAATTAGGTTATTCAGATATTTCGATGTACAAt                                                    <  2:56784/141‑1 (MQ=255)

                                                                               tttttATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTT‑TCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATgg                                                  <  1:283099/141‑2 (MQ=255)

                                                                                ttgtatataGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTT‑TCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTGATTCAGATATTTCGATGTACAATggc                                                 <  2:206403/138‑3 (MQ=255)

                                                                                 tttATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTT‑TCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACa                                                <  2:272027/141‑1 (MQ=255)

                                                                                   tatataGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTT‑TCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGt                                              <  1:310215/141‑1 (MQ=255)

                                                                                    atataGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTT‑TCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTc                                             <  1:275112/141‑1 (MQ=255)

                                                                                      ttaGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTT‑TCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAg                                           <  2:309578/140‑1 (MQ=255)

                                                                                       cagactggaaaCACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTT‑TCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAgg                                          <  1:155717/133‑1 (MQ=14)

                                                                                        agagCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTT‑TCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgt                                         >  1:280093/1‑141 (MQ=255)

                                                                                        agagCGTAAATACACTCAATACATTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTT‑TCTTACTTTAATATGACGTTGATCTTGCTCATTGCAGTTATTCAGATATTTCGATGTACAATGGCAGACAGgt                                         >  2:44202/1‑141 (MQ=14)

                                                                                         gagCGTAAATACATTCAATACATTTTATAGGATTCTTTGCTGTATTGATACTTTGATACCTTGTCTT‑TCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtt                                        >  2:322392/1‑141 (MQ=255)

                                                                                          agCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATCCTTTGATTCCTTGTCTT‑TCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgttt                                       <  1:246192/141‑1 (MQ=255)

                                                                                           gCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTT‑TCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgttta                                      <  1:176410/141‑1 (MQ=255)

                                                                                           gCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTT‑TCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtt                                        >  2:284054/1‑139 (MQ=255)

                                                                                             ttAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTT‑TCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaag                                    <  1:149773/140‑1 (MQ=255)

                                                                                             gTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTT‑TCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaag                                    <  2:132981/141‑1 (MQ=255)

                                                                                                aaTACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTT‑TCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagttt                                 >  1:204924/1‑141 (MQ=255)

                                                                                                aaTACACTTAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTT‑TCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagttt                                 <  2:129071/141‑1 (MQ=255)

                                                                                                aaTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTT‑TCTTACTTTAATATGAAGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCCGgtttaagttt                                 >  2:145367/1‑141 (MQ=255)

                                                                                                 aTACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTT‑TCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagttta                                >  1:169799/1‑141 (MQ=255)

                                                                                                  tacacttaaTACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTTTT‑TCTTACTTTAATATGACGGTGATCTTGCTCTATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagtttaa                               >  2:209707/8‑141 (MQ=14)

                                                                                                  tACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTT‑TCTTACTTTAATATGACGGTCATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagtttaa                               <  2:222816/141‑1 (MQ=255)

                                                                                                  tACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTT‑TCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGgtttaagtttaa                               >  1:102723/1‑141 (MQ=255)

                                                                                                  tACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTT‑TCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCCGTCAGgtttaagtttaa                               >  1:4759/1‑141 (MQ=255)

                                                                                                      ctCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTT‑TCTTACTTTAATATGACGGTGATCTTGCTCAATGCGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTATaaaact                           >  2:163233/2‑139 (MQ=255)

                                                                                                      ctCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTT‑TCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGc                           >  1:232559/2‑141 (MQ=255)

                                                                                                       tCAATACCTTTTAAAGTACACTACAATTTATTTATACTTTGATATCTTGTCTT‑TCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCt                          >  2:153061/1‑141 (MQ=14)

                                                                                                         aaTACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTATT‑TCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCttt                        >  2:19054/1‑141 (MQ=255)

                                                                                                           tACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTT‑TCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTaa                      <  1:261854/141‑1 (MQ=255)

                                                                                                           tACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTT‑TCTTACTTTATTATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTTAAAGCTTTaa                      >  1:298913/1‑141 (MQ=255)

                                                                                                            aCCTTTTAATGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTT‑TCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAAt                     <  2:278624/141‑1 (MQ=255)

                                                                                                            aCCTTTTAAAGTATTCTTTTCTGTATTGATACTTTGATACTTTGTCTT‑TCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCTGATAGTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAAt                     <  2:170636/141‑1 (MQ=14)

                                                                                                             ccTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTT‑TCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAAtt                    >  1:123639/1‑141 (MQ=255)

                                                                                                                 ttAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTT‑TCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACtt                >  1:214394/1‑141 (MQ=255)

                                                                                                                 ttAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTT‑TCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACtt                <  1:57237/141‑1 (MQ=40)

                                                                                                                 ttAAAGTATTCTTTGCCGTATTGATACTGTGATATCTTGTCTT‑TCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTattt                <  1:6680/141‑4 (MQ=255)

                                                                                                                    aaGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTT‑TCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTaa             <  1:113023/141‑2 (MQ=255)

                                                                                                                        aTTCTTTGCTGTATTGATACTTTGATACCTTGTCTT‑TCTTACTTTAATATGACGGTGATCTTGTTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCAt         >  2:315579/1‑141 (MQ=255)

                                                                                                                        aTTCTTTGCTGTATTGATACTTTGATACCTTGTCTT‑TCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTCCTTTAACCAt         >  1:302762/1‑141 (MQ=255)

                                                                                                                         ttCTTTGCCGTATTGATACTTTGATATCTTGTCTT‑TCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCAtt        <  1:296928/141‑1 (MQ=255)

                                                                                                                          tCTTCGCTGAATCGTTACTTTGATACCTTGGTTT‑TTTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATTTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTg       <  1:193257/141‑1 (MQ=14)

                                                                                                                            ttttCTGTATTGATACTTTGATACCTTGTCTT‑TCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATACTTCGCTGTACAATGGCCGTCAGGTTTAAGTTTAAAAGCTTTACTTACTTTAACCATTGCt     <  2:138666/141‑1 (MQ=14)

                                                                                                                            tttGCTGTATTGATACTTACATCCCTTGTCTT‑TCTTACGTTAATATGACGGTGCTCTTGCTCAATGAGGTTATTCAGATATTCCGATGTACAACTTCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCt     <  1:28984/141‑1 (MQ=11)

                                                                                                                              tGCTGTCTTGATACTTTTATCCCTTGTCGT‑TCTTACTTTAATACGACGGTGATCTTGCTCAATGAGGTTAGTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTAc   <  2:175989/141‑1 (MQ=14)

AGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTT‑TCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACC  >  CP000730/35868‑36135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`