BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	2,774,969	A→G	6.6%	D739D (GAT→GAC)	clfB ←	clumping factor B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,774,969	0	A	G	6.6%	314.8 / 3.8	132	D739D (GAT→GAC)	clfB	clumping factor B
Reads supporting (aligned to +/- strand): ref base A (60/59); new base G (3/9); total (64/68)
Fisher's exact test for biased strand distribution p-value = 1.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 135 total aligned reads displayed.

CTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATC  >  CP000730/2774829‑2775109

  gTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATc                                                                                                                                             <  1:374544/141‑1 (MQ=255)

  gTCCGAATCTTAATCACTGTCTGAATGCGAATCGCCATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATc                                                                                                                                             <  1:847969/141‑1 (MQ=255)

      gaatctgaatcACTGTCTGGATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGCCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTa                                                                                                                                         <  1:4945/141‑1 (MQ=255)

      gaatctgaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCtt                                                                                                                                         >  1:721536/1‑140 (MQ=255)

         tctgaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCCAATCGCGATCTgagtctgagtcgctgtctgagtctgagtcgctgtct                                                                                                                                      >  2:411801/1‑141 (MQ=17)

           tgaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctga                                                                                                                                    >  1:767414/1‑141 (MQ=255)

            gaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaa                                                                                                                                   >  1:30930/1‑141 (MQ=255)

             tatcACTGTCTGAATCCGGATCGCTATTTGAATCTGGAGTGCTTTCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgagt                                                                                                                                  <  2:664177/140‑3 (MQ=17)

             aatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaat                                                                                                                                  <  1:221570/141‑1 (MQ=255)

                cACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaatc‑tg                                                                                                                              <  1:459091/141‑1 (MQ=255)

                cACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaatc‑tg                                                                                                                              <  2:497965/141‑1 (MQ=255)

                 aCTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaatc‑tga                                                                                                                             <  1:819259/141‑1 (MQ=255)

                  cTGTCTGACTCCGAGTCGCGCTCTGAATCGGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAg                                                                                                                            <  2:187482/141‑1 (MQ=14)

                     tCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCg                                                                                                                         >  2:492527/1‑141 (MQ=255)

                      cTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGc                                                                                                                        <  1:863176/141‑1 (MQ=255)

                      cTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGc                                                                                                                        >  1:316837/1‑141 (MQ=255)

                             cgtaTCGCTATCTGATTCTGAGTCGCTCGCCGAGTCTGAGTCGCGGTCTGAATCTGACTCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTAtctga                                                                                                                 <  2:633329/138‑1 (MQ=11)

                              gAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTAtctgag                                                                                                                >  1:823612/1‑141 (MQ=255)

                               aaTCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGGCGCTAtctgagt                                                                                                               >  1:813086/1‑141 (MQ=255)

                                aTCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTAtctgagtc                                                                                                              <  1:652017/141‑1 (MQ=255)

                                aTCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCACTAtctgagtc                                                                                                              >  2:586835/1‑141 (MQ=255)

                                 tCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTGtctgagtct                                                                                                             >  2:740324/1‑141 (MQ=255)

                                   gCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTAtctgagtctga                                                                                                           >  1:300448/1‑141 (MQ=255)

                                    gattctgaatctgaaTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAGTC‑TGAGTCGCTGtctgagtcagag                                                                                                          >  2:821792/4‑141 (MQ=14)

                                    cTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTAtctgagtctgag                                                                                                          >  1:359617/1‑141 (MQ=255)

                                         tgaatctgaatcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTAtctgaggctgagtcgct                                                                                                     >  2:66935/1‑141 (MQ=255)

                                         tgaatctgaatcGCCATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTAtctgagtctgagtcgct                                                                                                     <  1:62174/141‑1 (MQ=255)

                                             tctgaatcGCTATCCGAGTCTGAGTCGCTGTCTGAAGCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTGtctgggtctgagtcgctgtct                                                                                                 >  1:322528/1‑141 (MQ=255)

                                                gaatcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTAtctgagtctgagtcgctgtctgag                                                                                              >  2:807683/1‑141 (MQ=255)

                                                gaatcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCGGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑GGAAGCGCTAtgtgggtcggagtcgctgtctgaa                                                                                              >  2:64667/1‑140 (MQ=17)

                                                  atcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTAtctgagtctgagtcgctgtctgagtc                                                                                            <  1:763409/141‑1 (MQ=255)

                                                     gCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTAtctgagtctgagtcgctgtctgagtctga                                                                                         <  1:492527/141‑1 (MQ=255)

                                                      cTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGCATC‑TGAGTCGCTAtctgagtctgagtggctgcctgagtctgag                                                                                        >  1:108505/1‑141 (MQ=255)

                                                       tATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTAtctgagtctgcgtcgctgtctgagtctgagt                                                                                       >  1:602518/1‑141 (MQ=255)

                                                       tATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTAtctgagtctgagtcgctgtctgagtctgagt                                                                                       >  1:510418/1‑141 (MQ=255)

                                                         tCCGATTCTGAGTCGCTGTCTTTATCTGAATCGCTGTCTGAGTCCGAATCGCTATCCGAATCGGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTAtctgagtctgagtcgctgtctgagtctgagtcg                                                                                     <  2:141799/141‑1 (MQ=14)

                                                         tCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATC‑TGAGTCGCTGtctgagtctgagtctctgtctgagtctgagtcg                                                                                     >  2:215198/1‑141 (MQ=255)

                                                           tgggTCTGGGTCGCTGTCTGAATCTGTATCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATC‑TGAGTCGCTAtctgagtctgagtcgctgtctgagtctgagtcgct                                                                                   <  2:105667/138‑1 (MQ=14)

                                                           cGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTAtctgagtctgagtcgctgtctgagtctgagtcgct                                                                                   <  1:17555/141‑1 (MQ=255)

                                                                  gAGTCGCTGTCTGAATCTGTATCGCTGTCTGAGTCCGTATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGtctgagtcgctgtctgagtctgagtcgctgtctgag                                                                            <  2:664547/141‑1 (MQ=255)

                                                                    gTCGCTGTCTGCATCTGACTCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCtgagtcgctgtctgagtctgagtcgctgtctgagtc                                                                          <  2:560581/141‑1 (MQ=255)

                                                                    gTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCtgagtcgctgtctgagtctgagtcgctgtctgagtc                                                                          <  1:764707/141‑1 (MQ=255)

                                                                       ccttccttgatctgaaccGCTGTCTCGATCCGAATCGCTACCTGGATCTGACTCGCTTTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAgtcgctgtctgagtctgagtcgctgtctgagtctga                                                                       <  2:810913/132‑1 (MQ=14)

                                                                        cTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGaa                                                                      <  1:96882/141‑1 (MQ=255)

                                                                           tctgaatctgaaTCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCg                                                                   >  1:448689/1‑141 (MQ=255)

                                                                               aatctgaatcGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTat                                                               <  1:784356/141‑1 (MQ=255)

                                                                                 tctgaatcGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCACTGTCTGAATCCGAATccggatcc                                                             <  2:391134/141‑8 (MQ=14)

                                                                                  ctgaatcGCTGTCTGAGTCCGAATCGCTATCTGAATGTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTatctg                                                            >  1:630738/1‑141 (MQ=255)

                                                                                   tgcatagCTGTCGGAGTCCGAGCCGCTCTCTGAATCTGAGTCGCTGTCTGGGTCTGAGTCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatctga                                                           <  2:219840/135‑1 (MQ=14)

                                                                                     aatcGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaat                                                         <  1:512544/141‑1 (MQ=255)

                                                                                         gCTGTCTGAGTCCGACTCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaatctga                                                     <  1:618614/141‑1 (MQ=255)

                                                                                         gCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaatctga                                                     <  1:443421/141‑1 (MQ=255)

                                                                                          cTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAg                                                    >  1:43843/1‑141 (MQ=255)

                                                                                          cTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAg                                                    >  1:459497/1‑141 (MQ=255)

                                                                                          cTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTAGCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCTCTGTCTGAGTCTGAACCGCTATCTACATCTGAg                                                    >  2:700497/1‑141 (MQ=14)

                                                                                            gtcggaagccGATTTGCTATGTGAATCTGAGTCGCTGTTTGATTCTGAATCGCTAATTGAATC‑TGAGTAGCTATCTTAGTCTTAGTCGATGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTc                                                  <  1:385311/133‑1 (MQ=17)

                                                                                             tCTGAGTCCGAGTCGCTCTCGGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCg                                                 <  2:595228/141‑1 (MQ=255)

                                                                                             tCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCg                                                 <  1:726621/141‑1 (MQ=255)

                                                                                                 aGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATC‑TGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGGATCTGAGTAGCTgt                                             >  2:448505/1‑141 (MQ=255)

                                                                                                 aGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTgt                                             >  1:127371/1‑141 (MQ=255)

                                                                                                  gTCCGACTCGCTATCTGCATCTGAGTCGCTGTCTGAGTCTGAATCGCTTTCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTgtc                                            <  2:794392/141‑1 (MQ=255)

                                                                                                  gTCCGAATCGCTCTCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTgtc                                            <  2:803842/141‑1 (MQ=255)

                                                                                                    ccGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTgtctg                                          <  1:871809/141‑1 (MQ=255)

                                                                                                    ccGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTgtctg                                          <  1:782279/141‑1 (MQ=255)

                                                                                                     cGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGACGCTTTCTGAGTCTGAATCGCTAGATGAGTCGGAGTCGCTgtctga                                         >  2:705469/1‑141 (MQ=17)

                                                                                                      gactCGCCATCTGACTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTgtctgag                                        <  2:823612/138‑1 (MQ=255)

                                                                                                        aTCGCTATTTGAATCTGTGGCGCCGTCTGAGTCTGAGTCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTgtctgagtc                                      <  2:310364/141‑1 (MQ=17)

                                                                                                        aTCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTgtctgagtc                                      >  1:781977/1‑141 (MQ=255)

                                                                                                         tCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTGTCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTgtctgagtct                                     <  2:316837/141‑1 (MQ=255)

                                                                                                         tCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTgtctgagtct                                     >  1:25006/1‑141 (MQ=255)

                                                                                                          cGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAGTCGCTgtctgagtctg                                    <  1:594456/141‑1 (MQ=255)

                                                                                                           gCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTgtctgagtatga                                   <  1:66935/141‑1 (MQ=255)

                                                                                                            ctggctgaatcgcgGTCGCTGTCTGAGTCTGAGCCGCTCTCTGAATC‑GGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGGCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGGGTCGCTGtctgagtctgag                                  <  2:509266/137‑1 (MQ=17)

                                                                                                            cTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGTTGC‑CGAGGCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGtctgagtctgag                                  <  1:852936/141‑1 (MQ=17)

                                                                                                            cTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGtctgagtctgag                                  <  1:161339/141‑1 (MQ=255)

                                                                                                            cTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGtctgagtctgag                                  <  1:634574/141‑1 (MQ=255)

                                                                                                              atctgaatctgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAATCTGAGTCGCTGTCtgagtctgagtc                                <  2:307544/141‑1 (MQ=255)

                                                                                                               tctgaatctgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcg                               >  2:703727/1‑141 (MQ=255)

                                                                                                               tctgaatctgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTATCAGAATCTGAGTCGCCGTCTGAGtctgagtcg                               >  2:703889/1‑141 (MQ=255)

                                                                                                               tctgaatctgaGTCGCTGTATCAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGACTGAATCGCTATCTGAAGCTGAGTCGCTGTCAGAGtccgagtcg                               >  2:196777/1‑141 (MQ=14)

                                                                                                                gtgaagctggGTCGCTGTCTGGGTCTGAGTGGCTATCTGGATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgc                              <  1:5143/140‑1 (MQ=17)

                                                                                                                 tgaatctgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGATTCTGAGTCGCTGGCTGTGTCTGAATCGGTGTCTGAATGTGAGTCACGAGCTGAGtgtgtgtcgat                             >  2:712968/1‑139 (MQ=14)

                                                                                                                  tgagcggcgTCGCTGTGCTAGTCGTAATCTCTATCTGAGTC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAATTGCTGTCTGAGTCTGAATCGCGATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctg                            <  2:219848/133‑1 (MQ=14)

                                                                                                                  gaatctgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGGGgctgagtcgctg                            >  2:853124/1‑141 (MQ=255)

                                                                                                                  gaatctgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctg                            <  1:604883/141‑1 (MQ=255)

                                                                                                                     tctgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTGTGCGactgagtcgctgtct                         >  1:51698/1‑141 (MQ=255)

                                                                                                                      ctgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctg                        <  2:107637/141‑1 (MQ=255)

                                                                                                                      ctgaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAATCGCTGTCTGATTCTGAATCGCTTTCTGAATCTGATTCGCTGTGTGAGtccgagtcgctcacta                        >  2:762807/1‑136 (MQ=14)

                                                                                                                        gaGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaa                      >  2:773689/1‑141 (MQ=255)

                                                                                                                         aGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTGTCTGCGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGGATCTGAGTGGCTGTCTGGGtctgagtcgctgtctgagt                     >  2:869871/1‑139 (MQ=14)

                                                                                                                         aGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgagt                     >  1:142096/1‑139 (MQ=255)

                                                                                                                            cGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctg                  <  1:807683/141‑1 (MQ=255)

                                                                                                                            cGCTGCCCGAGTCTGAATCGCTATCTGAATC‑TGAGCCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctg                  <  1:873645/141‑1 (MQ=255)

                                                                                                                              cTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgag                >  1:121793/1‑141 (MQ=255)

                                                                                                                              cTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCGGTCTGAGACTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtggatgactagatctgat                >  1:340053/1‑140 (MQ=14)

                                                                                                                               tttctgagtcggaATCGGTCTCTGACTC‑TGCGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGACTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagt               <  1:271769/139‑1 (MQ=14)

                                                                                                                                   tctctctgaATCGCTATCTGACTC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcgct           <  2:873448/137‑1 (MQ=255)

                                                                                                                                      gtctgaATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGtctgaggcgctgtctgaatctgagtcgctgtc        >  1:184694/1‑141 (MQ=255)

                                                                                                                                        ccgaATCGCTTTCTGAATCTTGTGT‑TCCACCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGACTCTGTATCGCTATCTGAAGCTGAGTCGCTGTCTGAGtctgagtcgctgtctgaatctgagtcgctgtctg      <  2:185894/139‑1 (MQ=14)

                                                                                                                                            aTCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGGCTGAGTCTGAATCGCTGTCTGGGTCTGATTCGCTATCTGCGtctgagtcgctgtctggggttgagtggctgtcggcagg  >  2:127237/1‑135 (MQ=11)

CTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATC‑TGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAATC  >  CP000730/2774829‑2775109

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`