Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	2,279,705	G→A	100%	intergenic (+1373/+6659)	mtlD → / ← glmM	mannitol‑1‑phosphate 5‑dehydrogenase/phosphoglucosamine mutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,279,705	0	G	A	100.0%	247.1 / NA	80	intergenic (+1373/+6659)	mtlD/glmM	mannitol‑1‑phosphate 5‑dehydrogenase/phosphoglucosamine mutase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (40/40);  total (40/40)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTGCTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCTGT  >  CP000730/2279568‑2279841                                                                                                                                          |                                                                                                                                          ttGAGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACtg                                                                                                                                        <  2:3825/141‑1 (MQ=255)    tgCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACtgttg                                                                                                                                     >  1:740323/1‑141 (MQ=255)    tgCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACtgttg                                                                                                                                     >  1:282638/1‑141 (MQ=255)       cGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGttt                                                                                                                                  >  1:132206/1‑141 (MQ=255)          tattaGTTGATTCTACTTCTGGTTTACTTGTTTCATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑Ac                                                                                                                              <  1:124675/141‑1 (MQ=255)           attaGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑Acc                                                                                                                             <  1:135533/141‑1 (MQ=255)           attaGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑Acc                                                                                                                             >  1:278958/1‑141 (MQ=255)           attaGTTGATTCTAATTCTGGTTTACTAGTTACATCGTTATCCAGTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑Acc                                                                                                                             >  1:431446/1‑141 (MQ=255)             tcgTTGATTCTTCTTCTGGTTTACTAGTTTCATCGTTATCCTTTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTg                                                                                                                           <  2:694134/139‑1 (MQ=255)             taGTTGATTCTTCTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCCTCAGTGTCTGATGTTACTGTTGTTTC‑ACCTg                                                                                                                           <  2:169365/141‑1 (MQ=255)               gTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGtt                                                                                                                         >  2:61766/1‑141 (MQ=255)                ttGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTg                                                                                                                        <  1:341723/141‑1 (MQ=255)                    ttCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGc                                                                                                                    <  1:684386/141‑1 (MQ=255)                     tCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGAACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCAGCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGct                                                                                                                   >  1:14316/1‑140 (MQ=255)                      tttcTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCAt                                                                                                                  <  2:261763/138‑1 (MQ=255)                        aCTTCTGGTTTACTAGTTACGTCGGTATCCATTGTCGGACTGTGTGTTGATGCATCCTCACTTGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCa                                                                                                                <  1:64643/141‑1 (MQ=255)                        aCTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATcc                                                                                                                >  2:576963/1‑140 (MQ=255)                        aCTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCa                                                                                                                >  2:790170/1‑141 (MQ=255)                        aCTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCa                                                                                                                <  1:329217/141‑1 (MQ=255)                         cTTCTGGTTTACTAGTCCCAACACGATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAACTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGGTGTTACTGTTGTTTC‑ACCTGTTGCCGCATcgc                                                                                                               >  1:383669/1‑139 (MQ=255)                           tCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCTGCATCACta                                                                                                             >  1:648532/1‑141 (MQ=255)                           tCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACta                                                                                                             >  2:17845/1‑141 (MQ=255)                           tCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTCC‑ACCTGTTGCCGCATCACta                                                                                                             >  2:350971/1‑141 (MQ=255)                           tCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATAAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCAGCATGACta                                                                                                             >  2:636023/1‑141 (MQ=255)                            tgggTTTACTAGTTACATTGTTTTCCATTGTCGGACTGTTTGTTCATGCATCTACCCTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCGGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACtat                                                                                                            <  2:377984/139‑1 (MQ=255)                                tttACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTattatt                                                                                                        <  1:247754/141‑1 (MQ=255)                                  tACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTg                                                                                                      >  1:341140/1‑141 (MQ=255)                                  tACCAGTTACCGCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTg                                                                                                      <  1:730268/141‑1 (MQ=255)                                     tAGTTACATCTTTATCCATTGTCGGACTGTTTGTTGCTGCATCTACACTAGTATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTTATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGgtg                                                                                                   <  1:353825/141‑1 (MQ=255)                                        ttACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTgttgttt                                                                                                >  1:335796/1‑140 (MQ=255)                                          acttCGTTTTCCATTGTCGGACTGTTTGTTGAGGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCAGTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTc                                                                                              <  2:120826/138‑1 (MQ=255)                                          aCATCGTTATCCATGTTCGGATTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTc                                                                                              <  1:83173/141‑1 (MQ=255)                                             tCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATTATCTGTTGCTGATGTTACGTTTCTTTCGAGGTG‑TGCCGCATCACTATTATTTGGTGTTGTCGga                                                                                           <  2:556025/141‑1 (MQ=255)                                               gTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGgaga                                                                                         >  1:249172/1‑141 (MQ=255)                                                     cATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGGGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTc                                                                                   <  2:359343/141‑1 (MQ=255)                                                      aTTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTAGTCGGAGAAGCGTCt                                                                                  >  1:92940/1‑141 (MQ=255)                                                       ttGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCCTCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTg                                                                                 >  1:303156/1‑141 (MQ=255)                                                       ttGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTg                                                                                 >  1:51089/1‑141 (MQ=255)                                                           cGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCttt                                                                             <  1:398293/141‑1 (MQ=255)                                                            ggccTGTTTGTTGGTGCATCTACACTAGAATTGTTATTAGATTGGGGTTTATCATGTGCATCGTCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCGCTATTATTTGGTGTTGTGGGCGCACCGTCTGCTTTg                                                                            >  2:360839/4‑141 (MQ=255)                                                               cTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTTTGGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCa                                                                         <  2:281749/141‑1 (MQ=255)                                                               cTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCACCAGTTGCTGATGCTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCa                                                                         <  2:281755/141‑1 (MQ=255)                                                                tgtttgttGATGCATCTACACTAGGATTGTTTCTAGCTTGCGGTTCATCATTTGCATCACCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTTTTTGAAATTGTCGTAGAAGCGTCTGCTTTGCCAt                                                                        <  1:189256/141‑1 (MQ=255)                                                                tgtttgttGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCAt                                                                        >  2:678026/1‑141 (MQ=255)                                                                   ttgttgATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTTGGAGAAGCGTCTGCTTTGCCATTAg                                                                     >  1:493792/1‑141 (MQ=255)                                                                    tgttgATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGc                                                                    <  2:655982/141‑1 (MQ=255)                                                                      ttgATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑CCCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAGGCGTCTGCTTTGCCATTAGCTg                                                                  >  1:135986/1‑141 (MQ=255)                                                                         aTGCATCTACACTAGAATTGATATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTgtcg                                                               >  1:475503/1‑141 (MQ=255)                                                                           gCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTgtcgtc                                                             <  2:266722/141‑1 (MQ=255)                                                                                tACACTAGAATTGTTATTGGCTTGCGGTTTATCCTTTGTATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGa                                                        <  2:208401/141‑1 (MQ=255)                                                                                tACACTAATATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACGATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGa                                                        <  1:370262/141‑1 (MQ=255)                                                                                      atgatgtttcttcgCTTCCGGTTTATCATTTGTATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGtt                                                  <  1:57837/128‑1 (MQ=255)                                                                                         aTTGTTATTAGCTTCCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAgg                                               <  2:648532/141‑1 (MQ=255)                                                                                              tattaGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTc                                          >  2:75162/1‑141 (MQ=255)                                                                                                   gCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCTTTAGCTGTCGTGTCAGATACGTTAGGTTGTCCGGtt                                     >  2:493215/1‑140 (MQ=255)                                                                                                   gCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGGTAGGTTGTCCAGtt                                     >  1:2750/1‑140 (MQ=255)                                                                                                        cGGTTTATCTTTTGCTTCATCAGTTGCTGTTGTTACTGTTGTTTC‑TCCTTTTGCCGCATCACTATTATTTGGTGTTGTCGGAGATGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTc                                <  2:417296/141‑1 (MQ=255)                                                                                                         ggTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCACATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCt                               >  1:525038/1‑141 (MQ=255)                                                                                                          gTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTg                              <  2:132206/141‑1 (MQ=255)                                                                                                           tttGTCATTTGCTTCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTgg                             <  1:288158/141‑1 (MQ=255)                                                                                                            ttATCATTTGCATCATCCGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGgt                            <  1:177933/141‑1 (MQ=255)                                                                                                            ttATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTATGgt                            >  1:686045/1‑141 (MQ=255)                                                                                                              aTCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGgtgt                          <  2:393181/141‑1 (MQ=255)                                                                                                                  ttttcgtcggtaGTTGCTGATGTTACTGTTGTTTC‑GCCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCa                      <  1:197897/130‑1 (MQ=255)                                                                                                                  tttGCATCATTCGTTGCCGATGTTACTGTTGTTTC‑CCCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCCCAAACTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTCGCa                      <  1:615241/141‑1 (MQ=255)                                                                                                                       atcatcAGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGc                 >  1:765203/1‑141 (MQ=255)                                                                                                                           tcaGTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCAttt             <  1:430862/141‑1 (MQ=255)                                                                                                                           gcaactgaagATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTAGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCAGTCGTCTCAGATACGTTAGGTTGACCAGTATTTTCTGGTGTTGCATTAGCAttt             >  2:581127/10‑141 (MQ=255)                                                                                                                            caGTTGCTGATGTTACTGTTGTTTC‑ACGTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGCTACGTTAGGTTGTACAGTATTTTCTGGTGTTGCATTAGCGTTTg            >  2:459753/1‑141 (MQ=255)                                                                                                                              gTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGTGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTTGGTTGTCAAGTATTTTCTGGTGTTGCATTAGCATTTGaa          >  1:19852/1‑141 (MQ=255)                                                                                                                              gTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGaa          <  1:790170/141‑1 (MQ=255)                                                                                                                              gTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGaa          >  1:640190/1‑141 (MQ=255)                                                                                                                              gTTGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTATGGTGTTGCATTAGCATTTGaa          >  2:516185/1‑141 (MQ=255)                                                                                                                               ttGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCGTTAGCATTTGAAt         >  2:291573/1‑141 (MQ=255)                                                                                                                               ttGCTGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAAt         <  2:303156/141‑1 (MQ=255)                                                                                                                                   tGATGTTACTGTTGTTTC‑GCCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGc     <  1:395133/141‑1 (MQ=255)                                                                                                                                   tGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCCGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGc     >  2:415613/1‑141 (MQ=255)                                                                                                                                   tGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGc     <  1:622414/141‑1 (MQ=255)                                                                                                                                   tGATGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGc     >  2:234743/1‑141 (MQ=255)                                                                                                                                      tGTTACTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGAATTAGCATTTGAATTTGCTGt  >  2:593761/1‑141 (MQ=255)                                                                                                                                          |                                                                                                                                          TTGTGCCGTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTGCTGTTGTTTC‑ACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAATTTGCTGT  >  CP000730/2279568‑2279841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑