BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	466,487	T→A	3.9%	S163R (AGT→AGA)	lpl7 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	466,487	0	T	A	3.9%	353.1 / 2.6	132	S163R (AGT→AGA)	lpl7	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (65/59); new base A (4/2); total (69/63)
Fisher's exact test for biased strand distribution p-value = 6.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 132 total aligned reads displayed.

TCAGGACGTTTTATCGCAAGGATAAATTACCTGATAGAAAAAATTATAAAGTTGAAATGAAAAATAATAAAATCATTTTATTAGATAAAGTAGAAGATAAAAAGC‑TTAAACAAAAAATAGAAAATTTTAAATTTTTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GGTGATGTCT‑CAATTAATGAGAATGTTCCAAGTTATGACGTGAAATATAAAATGAGTAATAAAGATGAAAATGTTAAGCAATTAAGAAGTCGTTATAAT  >  CP000730/466348‑466625

      ggagttagcgggaGGATAAATTATCTGAAAGAAAAAAATAGAAAGGTGAAATGAAAAATAATAAAATCATTTTATTAGATAAAGTAGAAGATAAAAAAC‑AAAAAAAAAAAATAGAAAATTTTAAATTTTTCAGTCAATATg                                                                                                                                       <  2:262830/129‑1 (MQ=17)

        ttttATCGCAAGGATAAATTACCTGATAGAAAAAATTATAAAGTTGAAATGAAAAATAATAAAATCATTTTATTAGATAAAGTAGAAGATAAAAAGC‑TTAAACAAAAAATAGAAAATTTTAAATTTTTCAGTCAATATGCa                                                                                                                                     >  1:749011/1‑141 (MQ=255)

           tATCGCAAGGATAAATTACCTGATAGAAAAAATTATAAAGTTGAAATGAAAAATAATAAAATCATTTTATTAGATAAAGTAGAAGATAAAAAGC‑TTAAACAAAAAATAGAAAATTTTAAATTTTTAAGTCAATATGAAAAt                                                                                                                                  >  2:229392/1‑141 (MQ=255)

            aTCGCAAGGATAAATTACCTGATAGAAAAAATTATAAAGTTGAAATGAAAAATAATAAAATCATTTTATTAGATAAAGTAGAAGATAAAAAGC‑TTAAACAAAAAATAGAAAATTTTAAATTTTTCAGTCAATATGCAAATc                                                                                                                                 >  2:18502/1‑141 (MQ=255)

            aTCGCAAGGATAAATTACCTGATAGAAAAAATTATAAAGTTGAAATGAAAAATAATAAAATCATTTTATTAGATAAAGTAGAAGATAAAAAGC‑TTAAACAAAAAATAGAAAATTTTAAATTTTTCAGTCAATATACAAATc                                                                                                                                 >  2:441969/1‑141 (MQ=255)

             tCGAAAGGATAAATTACCTGATAGAAAAAATTATAAAGTTGAAATGAAAAATAATAAAATCATTTTATTAGATAAAGTAGAAGATAAAAAGC‑TTAAACAAAAAATAGAAAATTTTAAATTTTTCAGTCAATATGCAAATCt                                                                                                                                <  1:231827/141‑1 (MQ=255)

                  aGGATAAATTACCTGATAGACAAGCGAATAAAGTTGAAATGAAAAATAATAAAATCATTTTATTAGATAAAGTAGAAGATAAAAAGC‑TTAAACAAAAAATAGAAAATTTTAAATTTTTCAGTCAATATGCAAATCTTAAAg                                                                                                                           >  2:1068725/1‑141 (MQ=21)

                  aGGATAAATTACCTGATAGAAAAAATTATAAAGTTGAAATGAAAAATAATAAAATCATTTTATTAGATAAAGTAGAAGATAAAAAGC‑TTAAACAAAAAATAGAAAATTTTAAATTTTTCAGTCAATATGCAAATCTTAAAg                                                                                                                           >  1:576020/1‑141 (MQ=255)

                     aTAAATTACCTGATAGAAAAAATTATAAAGTTGAAATGAAAAATAATAAAATCATTTTATTAGATAAAGTAGAAGATAAAAAGC‑TTAAACAAAAAATAGAAAATTTTAAATTTTTCAGTCAATATGCAAATCTTAAAGAAt                                                                                                                        <  2:98055/141‑1 (MQ=255)

                      tAAATTACCTGATAGAAAAAATTATAAAGTTGAAATGAAAAATAATAAAATCATTTTATTAGATAAAGTAGAAGATAAAAAGC‑TTAAACAAAAAATAGAAAATTTTAAATTTTTCAGTCAATATGCAAATCTTAAAGAAtt                                                                                                                       <  1:977408/141‑1 (MQ=255)

                        aaTTACCTGATAGAAAAAATTATAAAGTTGAAATGAAAAATAATAAAATCATTTTATTAGATAAAGTAGAAGATAAAAAGC‑TTAAACAAAAAATAGAAAATTTTAAATTTTTCAGTCAATATGCAAATCTTAAAGAATTGa                                                                                                                     >  2:490591/1‑141 (MQ=255)

                         aTTACCTGATAGAAAAAATTATAAAGTTGAAATGAAAAATAATAAAATCATTTTATTAGATAAAGTAGAAGATAAAAAGC‑TTAAACAAAAAATAGAAAATTTTAAATTTTTCAGTCAATATGCAAATCTTAAAGAATTGaa                                                                                                                    >  1:492294/1‑141 (MQ=255)

                              cTGATAGAAAAAATTATAAAGTTGAAATGAAAAATAATAAAATCATTTTATTAGATAAAGTAGAAGATAAAAAGC‑TTAAACAAAAAATAGAAAATTTTAAATTTTTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACt                                                                                                               >  2:175333/1‑141 (MQ=255)

                              cTGATAGAAAAAATTATAAAGTTGAAATGAAAAATAATAAAATCATTTTATTAGATAAAGTAGAAGATAAAAAGC‑TTAAACAAAAAATAGAAAATTTTAAATTTTTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACt                                                                                                               >  1:1079068/1‑141 (MQ=255)

                                gATAGAAAAAATTATAAAGTTGAAATGAAAAATAATAAAATCATTTTATTAGATCAAGTAGAAGATAAAAAGC‑TTAAACAAAAAATAGAAAATTTTAAATTTTTCAGTCACTATGCAAATCTTAAAGAATTGAAAAACTAc                                                                                                             >  1:714887/1‑141 (MQ=255)

                                  tAGAAAAAATTATAAAGTTGAAATGAAAAATAATAAAATCATTTTATTAGATAAAGTAGAAGATAAAAAGC‑TTAAACAAAAAATAGAAAATTTTAAATTTTTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTACAg                                                                                                           >  1:269360/1‑141 (MQ=255)

                                  tAGAAAAAATTATAAAGTTGAAATGAAAAATAATAAAATCATTTTATTAGATAAAGTAGAAGATAAAAAGC‑TTAAACAAAAAATAGAAAATTTTAAATTTTTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTAAAg                                                                                                           >  1:385286/1‑141 (MQ=255)

                                      aaaaaTTATAAAGTTGAAATGAAAAATAATAAAATCATTTTATTAGATAAAGTAGAAGATAAAAAGC‑TTAAACAAAAAATAGAAAATTTTAAATTTTTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTACAGCaaa                                                                                                       >  2:549900/1‑140 (MQ=255)

                                        aaaaTAAAAAGTTTAAATGAAAAATAAAAAAATCATTATAGTAGATAAGGTAGAAGATAAAAAAC‑TAAAACAAAAAATAGAAAATTTTAAATTTTTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑gg                                                                                                    <  2:373573/141‑1 (MQ=18)

                                        aaaTTATAAAGTTGAAATGAAAAATAATAAAATCATTTTATTAGATAAAGTAGAAGATAAAAAGC‑TTAAACAAAAAATAGAAAATTTTAAATTTTTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑gg                                                                                                    <  2:687550/141‑1 (MQ=255)

                                        aaaTTATAAAGTTGAAAAGAAAAATAAGAAAATCATTTTATTAGATAAAGTAGAAGATAAAAAGC‑TTAAACAAAAAATAGAAAATTTTAAATTTTTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTACAGCCAT‑gg                                                                                                    <  2:265399/141‑1 (MQ=255)

                                          aTTATAAAGTTGAAATGAAAAATAATAAAATCATTTTATTAGATAAAGTAGAAGATAAAAAGC‑TTAAACAAAAAATAGAAAATTTTAAATTTTTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GGTg                                                                                                  >  2:884477/1‑141 (MQ=255)

                                          aTTATAAAGTTGAAATGAAAAATAATAAAATCATTTTATTAGATAAAGTAGAAGATAAAAAGC‑TTAAACAAAAAATAGAAAATTTTAAATTTTTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GGTg                                                                                                  >  2:743730/1‑141 (MQ=255)

                                             ataAAGTTGAAATGAAAAATAATAAAATCATTTTATTAGATAAAGTAGAAGATAAAAAGC‑TTAAACAAAAAATAGAAAATTTTAAATTTTTCAGTTAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GGTGATg                                                                                               <  1:517034/141‑1 (MQ=255)

                                             ataAAGTTGAAATGAAAAATAATAAAATCATTTTATTAGATAAAATAAAAAATAAAAAGC‑TTAAACAAAAAATAGAAAATTTTAAATTTTTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GGTGATg                                                                                               <  2:268917/141‑1 (MQ=255)

                                             ataAAGTTGAAATGAAAAATAATAAAATCATTTTATTAAATAAAGTAGAAAATAAAAAGC‑TTAAACAAAAAATAGAAAATTTTAAATTTTTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTTCAGCAAT‑GGTGATg                                                                                               >  2:310964/1‑141 (MQ=255)

                                               aaaGTTGAAATGAAAAATAATAAAATCATTTTATGATATAAAGTAGAAGATAAAAAGC‑TTAAACAAAAAATAGAAAATTTTAAATTTTTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GGTGATGtc                                                                                             >  1:533531/1‑141 (MQ=255)

                                                 aGTTGAAATGAAAAATAATAAAATCATTTTATTAGATAAAGTAGAAGATAAAAAGC‑TTAAACAAAAAATAGAAAATTTTAAATTTTTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GGTGATGtct‑c                                                                                          <  1:960200/141‑1 (MQ=255)

                                                     gaaatgaaaAATAATAAAATCATTTTATTAGATAAAGTAGAAGATAAAAAGC‑TTAAACAAAAAATAGAAAATTTTAAATTTTTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GGTGATGTCT‑Caaaa                                                                                      >  1:793857/1‑139 (MQ=255)

                                                     gaaatgaaaAATAATAAAATCATTTTATTAGATAAAGTAGAAGATAAAAAGC‑TTAAACAAAAAATAGAAAATTTTAAATTTTTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GGTGATGTCT‑CAAtt                                                                                      >  1:145072/1‑141 (MQ=255)

                                                          gcaaaaTAATAAAATCATTATATTAGATAAAGTCGAAGATAAAAAGC‑TTAAACAAAAAACAGAAAAATTTAAATTTTTCAGTCAATAAGCAAATCTTACAGAATTGAAAAACTACAGCAAT‑GGTGATGTCT‑Cacataaaga                                                                                 >  2:366333/3‑133 (MQ=255)

                                                          gaaaAATAATAAAATCATTTTATTAGATAAAGTAGAAGATAAAAAGC‑TTAAACAAAAAATAGAAAATTTTAAATTTTTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GGTGATGTCT‑CAATTAATga                                                                                 <  2:53318/141‑1 (MQ=255)

                                                          gaaaAATAATAAAATCATTTTATTAGATAAAGTAGAAGATAAAAAGC‑TTAAACAAAAAATAGAAAATTTTAAATTTTTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GGTGATGTCT‑CAATTAATga                                                                                 <  2:476188/141‑1 (MQ=255)

                                                          gaaaAATAATAAAATCATTTTATTAGATAAAGTAGAAGATAAAAAGC‑TTAAACAAAAAATAGAAAATTTTAAATTTTTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GGTGATGTCT‑CAATTAATga                                                                                 <  1:359612/141‑1 (MQ=255)

                                                          agaagagaataaAATCATTTTATTAGATAAAGTAGAAGATAAAAAGC‑TGAAGCAAAAAATAGAAAATTTTAAATTGTTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GGTGATGTCT‑CAATTAATga                                                                                 <  2:385993/134‑1 (MQ=18)

                                                          aaaaaaTAATAAAATCATTTTATTAGATAAAGTAGAAGATAAAAAGC‑TTAAACAAAAAATAGAAAATTTTAAATTTTTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GGTGATGTCT‑CAATTAATga                                                                                 <  2:1015139/140‑1 (MQ=255)

                                                          aaaaaaTAATAAAATCATTTTATTAGATAAAGTAGAAGATAAAAAGC‑TTAAACAAAAAATAGAAAATTTTAAATTTTTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GGTGATGTCT‑CAATTAATga                                                                                 <  1:41098/140‑1 (MQ=255)

                                                            aaaaTAATAAAATCATTTTATTAGATAAAGTAGAAGATAAAAAGC‑TTAAACAAAAAATAGAAAATTTTAAATTTTTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GGTGATGTCT‑CAATTAATgaga                                                                               >  2:1048025/1‑141 (MQ=255)

                                                            aaaaTAATAAAATCATTTTATTAGATAAAGTAGAAGATAAAAAGC‑TTAAACAAAAAATAGAAAATTTTAAATTTTTCAGAAAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GGTGATGTCT‑CAATTAATgaga                                                                               <  1:480080/141‑1 (MQ=255)

                                                                  ataaAATCAGTTTATTAGATAAAGTAGAAGATAAAAAGC‑TTAAACAACAAATAGAAAATTTTAAATTTTTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTACATCAAT‑GGTGAGGTCT‑CAATTATTGAGATTGtta                                                                         >  2:709838/1‑140 (MQ=21)

                                                                    aaaaTCATTTTATTAGATAAAGTAGAAGATAAAAAGC‑TTAAACAAAAAATAGAAAATTTTAAATTTTTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GGTGATGTCT‑CAATTAATGAGAATGTTCCa                                                                       >  2:172557/1‑141 (MQ=255)

                                                                    aaaaTCATTTTATTAGATAAAGTAGAAGATAAAAAGC‑TTAAACAAAAAATAGAAAATTTTAAATTTTTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GGTAATGTCT‑CAATTAATGAGAATGTTCCa                                                                       >  2:525058/1‑141 (MQ=255)

                                                                    aaaaTCATTTTATTAGATAAAGGAGAAAATAAAAATC‑TTAAACAAAACATAGAAACTCTTAAATATTTCAATCAAGATGCAAAGCTTAAAGAATTGAAAAAATACAGCAAT‑GGTGATGTCT‑CAATGAAtaccaatggcaca                                                                       >  2:554717/1‑129 (MQ=255)

                                                                       aTCATTTTATTAGATAAAGTAGAAGATAAAAAGC‑TTAAACAAAAAATAGAAAATTTTAAATTTTTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GGTGATGTCT‑CAATTAATGAGAATGTTCCAAGt                                                                    <  1:930089/141‑1 (MQ=255)

                                                                         cATTTTATTAGATAAAGTAGAAGATAAAAAGC‑TTAAACAAAAAATAGAAAATTTTAAATTTTTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GGTGATGTCT‑CAATTAATGAGAATGTTCCAAGTTa                                                                  <  1:659852/141‑1 (MQ=255)

                                                                         cATTTTATTAGATAAAGTAGAAGATAAAAAGC‑TTAAACAAAAAATAGAAAATTTTAAATTTTTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GGTGATGTCT‑CAATTAATGAGAATGTTCCAAGTTa                                                                  <  2:737734/141‑1 (MQ=255)

                                                                              tattaGATAAGGTAGAATAAAAAAAGC‑TAAAAAAAAAAATAGAAAATTTTAATTTTTTAAGTCAATATGCAAATCTTAAAGAATTGAAAACCTAAGGCAAG‑GGTGTTGTCT‑CAATTAATGAGAATGTTCCAGGTTTTgcgg                                                             <  2:407327/141‑4 (MQ=17)

                                                                              aaaaaataaaaGGAGAAGAAAAAAAGC‑TTAAACAAAAAAAAAAAAATTTAAAATTTTTCGGCCAATATGCAAAAAAAAAAAAAAAAAAAAACTACAGAAAT‑GGTGATGTCT‑AAATTAATGAGAATGTTCCAAGTTATGACg                                                             <  2:544139/133‑1 (MQ=12)

                                                                               attaGATAAAGTAGAAGATAAAAAGC‑TTAAACAAAACATAGAAAATTTTAAATTTTTCAGTCAATATGCAAATCTTAAAGAATAGAGAGACTACAGCAAT‑GGTGATGTCT‑CAATTAATGAGAATGTTCCAAGTTATGACGt                                                            <  1:208656/141‑1 (MQ=255)

                                                                               ataaaataaaagaaaagaTAAAAAACTTTAAACAAAAAATAGAAAATTTAAAATTTTCAATTCAAATTGAAAATCTAAAAGAA‑TGGAAAACCACAGCAAA‑GGGTGTGTTTCCATTTAATGACATTGTTCGAAGTTATGCCg                                                             <  2:509917/127‑1 (MQ=255)

                                                                               agtaGAGACAGGAGAGGATAAAAAGC‑AGAAACAAAAAATAGAAAATTTTAAATTTTTCAGACAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GGTGATGTCT‑CAATTAATGGGAATGTTCCAAGTTATGACGt                                                            <  2:1047288/139‑1 (MQ=17)

                                                                                   gATAAAGTAGAAGATAAAAAGC‑TTAAACAAAAAATAGAAAATTTTAAATTTTTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GGTGATGTCT‑CAATTAATGAGAATGTTCCAAGTTATGACGTGaaa                                                        >  2:738495/1‑141 (MQ=255)

                                                                                   gATAAAGTAGAAGATAAAAAGC‑TTAAACAAAAAATAGAAAATTTTAAATTTTTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GGTGATGTCT‑CAATTAATGAGAATGTTCCAAGTTATGACGTGaaa                                                        >  2:87204/1‑141 (MQ=255)

                                                                                   gATAAAGTAGAAGATAAAAAGC‑TTAAACAAAAAATAGAAAATTTTAAATTTTTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GGTGATGTCC‑CAATTAATGAGAATGTTCCAAGTTATGACGTGaaa                                                        <  1:95185/141‑1 (MQ=255)

                                                                                    aTAAAGTAGAAGATAAAAAGC‑TTAAACAAAAAATAGAAAATTTTAAATTTTTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GGTGATGTCT‑CAATTAATGAGAATGTTCCAAGTTATGACGTGAAat                                                       <  1:598243/141‑1 (MQ=255)

                                                                                        aGTAGAAGATAAAAAGC‑ATAAACAAAAAATAGAAAATTTTAAATTTTTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GGTGATGTCT‑CAATTAATGAGAATGTTCCAAGTTATGACGTGAAATATaa                                                   <  1:906275/141‑1 (MQ=255)

                                                                                          tAGAAGATAAAGAGC‑TTAAACAAAAAATAGAAAATTTTAAATTTTTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GGTGATGTCT‑CAATTAATGAGAATGTTCCAAGTTATGACGTGAAATATaaaa                                                 >  1:901647/1‑141 (MQ=255)

                                                                                          tAGAAGATAAAAAGC‑TTAAACAAAAAATAGAAAATTTTAAATTTTTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GGTGATGTCT‑CAATTAATGAGAATGTTCCAAGTTATGACGTGAAATATaaaa                                                 >  1:318995/1‑141 (MQ=255)

                                                                                           agaagaTAAAAAGC‑TTAAACAAAAAATAGAAAATTTTAAATTTTTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GGTGATGTCT‑CAATTAATGAGAATGTTCCAAGTTATGACGTGAAATATAAAAt                                                <  2:276859/141‑1 (MQ=255)

                                                                                            gaagaTAAAAAGC‑TTAAACAAAAAATAGAAAATTTTAAATTTTTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GGTGATGTCT‑CAATTAATGAGAATGTTCCAAGTTATGACGTGAAATATAAAATg                                               >  1:104170/1‑141 (MQ=255)

                                                                                                aTAAAAAGC‑TTAAACAAAAAATAGAAAATTTTAAATTTTTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GGTGATGTCT‑CAATTAATGAGAATGTTCCAAGTTATGACGTGAAATATAAAATGAGta                                           >  1:782878/1‑141 (MQ=255)

                                                                                                 tAAAAAGC‑TTAAACAAAAAATAGAAAATTTTAAATTTTTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GGTGATGTCT‑CAATTAATGAGAATGTTCCAAGTTATGACGTGAAATATAAAATGAGtaa                                          <  2:210884/141‑1 (MQ=255)

                                                                                                 tAAAAAGC‑TTAAACAAAAAATAGAAAATTTTAAATTTTTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GGTGATGTCT‑CAATTAATGAGAATGTTCCAAGTTATGACGTGAAATATAAAATGAGtaa                                          <  2:650293/141‑1 (MQ=255)

                                                                                                 tAAAAAGC‑TTAAACAAAAAATAGAAAATTTTAAATTTTTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GGTGATGTCT‑CAATTAATGACAATGTTCCAAGTTATGACGTGGAATATAAAATGAGtaa                                          <  1:616287/141‑1 (MQ=255)

                                                                                                 aaaagaGC‑TTAAACAAAAAATAGAAAATGTGAAAGTTTTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GGTGATGTCT‑CAATTAATGAGAATGTTCCAAGTTATGCCGTGAAATATAAAATGAGtaa                                          <  2:581813/140‑1 (MQ=21)

                                                                                                   aaaaGC‑TTAAACAAAAAATAGAAAATTTTAAATTTTTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GGTGATGTCT‑CAATTAATGAGAATGTTCCAAGTTATGACGTGAAATATAAAATGAGtaata                                        >  1:362788/1‑141 (MQ=255)

                                                                                                   aaaaGC‑TTAAACAAAAAATAGAAAATTTTAAATTTTTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GGTGATGTCT‑CAATTAATGAGAATGTTCCAAGTTATGACGTGAAATATAAAATGAGtaata                                        >  1:169642/1‑141 (MQ=255)

                                                                                                   aaaaGC‑TTAAACAAAAAATAGAAAATTTTAAATTTTTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GGTGATGTCT‑CAATTAATGAGAATGTTCCAAGTTATGACGTGAAATATAAAATGAGtaata                                        >  1:865061/1‑141 (MQ=255)

                                                                                                   aaaaGC‑TTAAACAAAAAATAGAAAATTTTAAATTTTTCAGAGAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GGTGATGTCT‑CAATTAATGAGAATGTTCCAAGTTATGGAGGGAAATATAAAATGAGtaaga                                        >  1:315647/1‑139 (MQ=18)

                                                                                                   aaaaGC‑TTAAACAAAAAATAGAAAATTTTAAATTTTTCAGACAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GGTGATGTCT‑CAATTAATGAGAATGTTCCAAGTTATGACGTGAAATATAAAATGAGtaata                                        >  2:10218/1‑141 (MQ=255)

                                                                                                   aaaaGC‑TTAAACAAAAAATAGAAAATTTTAAATTTTTCAGACAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GGTGATGTCT‑CAATTAATGAGAAGGTTCCAAGTTATGACGGGAAATATAAAATGAGtaata                                        >  2:578209/1‑141 (MQ=255)

                                                                                                   aaaaGC‑TTAAACAAAAAATAGAAAATTTTAAATTTTTCAGACAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GGTGATGTCT‑CAATTAATGAGAAAGTTCCAAGTTATGACGTGAAATATAAAATGAGtaata                                        >  2:91251/1‑141 (MQ=255)

                                                                                                      aGC‑TTAAACAAAAAAAAGAAAATTTTAAATTTTGCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GGTGATGTCT‑CAATTAATGAGAATGTTCCAAGTTATGACGTGAAATATAAAATGAGTAATAAAg                                     <  1:15479/141‑1 (MQ=255)

                                                                                                        c‑TTAAACAAAAAATAGAAAATTTTAAATTTTTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GGTGATGTCT‑CAATTAATGAGAATGTTCCAAGTTATGACGTGAAATATAAAATGAGTAATAAAGAt                                   >  1:382198/1‑141 (MQ=255)

                                                                                                          ttAAACAAAAAATAGAAAATTTTAAATTTTTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GGTGATGTCT‑CAATTAATGAGAATGTTCCAAGTTATGACGTGAAATATAAAATGAGTAATAAAGATg                                  <  2:673721/141‑1 (MQ=255)

                                                                                                          atAAAAAAAAAATAGAAAAATTTAAATTTTGCACTCAAGATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GGGGATGTCT‑CAATTAATGAGAATGTTCCAAGTTATGACGTGAAATATAAAATGAGTAATAAAGATg                                  <  2:843988/140‑1 (MQ=18)

                                                                                                             aaCAAAAAATAGAAAATTTTAAATTTTTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GGTGATGTCT‑CAATTAATGAGAATGTTCCAAGTTATGACGTGAAATATAAAATGAGTAATAAAGATGaaa                               >  2:71774/1‑141 (MQ=255)

                                                                                                              aaaaaaaaTAGAAAATTTTAAATTTTTCAGTCAACAGGCCACTCTTCCACTGTCGAAAAACAACAGCAAT‑GGTGATGTCT‑CAAATAACGTGAATGTTCCAAGTTCTGACGTGAAATATAAAATGAGTAATAAAGATGaaaa                              <  2:335861/139‑1 (MQ=21)

                                                                                                              aCAAAAAATAGAAAATTTTAAATTTTTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GGTGATGTCT‑CAATTAATGAGAATGTTCCAAGTTATGACGTGAAATATAAAATGAGTAATAAAGATGaaaa                              >  1:89007/1‑141 (MQ=255)

                                                                                                                  aaaaTAGAAAATTTTAAATTTTTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GGTGATGTCT‑CAATTAATGAGAATGTTCCAAGTTATGACGTGAAATATAAAATGAGTAGTAAAGATGAAAATGtt                          >  2:297460/1‑141 (MQ=255)

                                                                                                                  aaaaTAGAAAATTTTAAATTTTTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GGTGATGTCT‑CAATTAATGAGAATGTTCCAAGTTATGACGTGAAATATAAAATGAGTAATAAAGATGAAAATGtt                          >  1:1012647/1‑141 (MQ=255)

                                                                                                                  aaaaTAGAAAATTTTAAATTTTTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GGTGATGTCT‑CAATTAATGAGAATGTTCCAAGTTATGACGTGAAATATAAAATGAGTAATAAAGATGAAAATGtt                          >  2:163823/1‑141 (MQ=255)

                                                                                                                  aaaaTAGAAAATTTTAAATTTTTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GGTGATGTCT‑CAATTAATGAGAATGTTCCAAGTTATGACGTGAAATATAAAATGAGTAATAAAGATGAAAATGtt                          >  1:503393/1‑141 (MQ=255)

                                                                                                                  aaaaTAGAAAATTTTAAATTTTTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GGTGATGTCT‑CAATTAATGAGAATGTTCCAAGTTATGACGTGAAACATAAAATGAGTAATAAAGATGAAAATGtt                          >  2:230188/1‑141 (MQ=255)

                                                                                                                   aaaTAGAAAATTTTAAATTTTTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GGTGATGTCT‑CAATTAATGAGAATGTTCCAAGTTATGACGTGAAATATAAAATGAGTAATAAAGATGAAAATGTTa                         >  2:933487/1‑141 (MQ=255)

                                                                                                                    aaTAGAAAATTTTAAATTTTTCAGTAAATATGCAAATCTTAAAGAATTGAAAAACT‑CATAATTGGGGGATGTCT‑CAATTAATGAGAATGTTCCAAGTTATGACGTGAAATATAAAATGAGTAATAAAGATGAAAATGTTaa                        <  1:525023/141‑1 (MQ=17)

                                                                                                                     aTAGAAAATTTTAAATTTTTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GATGATGTCT‑CAATTAATGAGAATGTTCCAAGTTATGACGTGAAATATAAAATGAGTAATAAAGATGAAAATGTTacg                       >  2:411677/1‑139 (MQ=255)

                                                                                                                     aTAGAAAATTTTAAATTTTTCAGTAAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GGTGATGTCG‑CAATTAATGAGAATGTTCCAAGTTATGACGTGAAATATAAAATGAGTAATAAAGATGAAAATGTTAAg                       >  1:808352/1‑141 (MQ=255)

                                                                                                                     aTAGAAAATTTTAAATTTGTCAGTGGATATGTAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GAGGATGTGT‑AAATTAATGAGAATGTTCAAAGTTATGACGTGAAAGATAAAATGAGTAATAAAGATGAAAATGTTAAg                       >  2:556736/1‑141 (MQ=17)

                                                                                                                      tAGAAAATTTTAAATTTTTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GGTGATGTCT‑CAATTAATGAGAATGTTCCAAGTTATGACGTGAAATATAAAATGAGTAATAAAGATGAAAATGTTAAGc                      >  1:875294/1‑141 (MQ=255)

                                                                                                                         aaaaTTTTAAATTTTTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTACAGCCAT‑GGTGATGTCT‑CAATTAATGAGAATGTTCCAAGTTATGACGTGAAATATAAAATGAGTAATAAAGATGAAAATGTTAAGCAAt                   >  1:307308/1‑141 (MQ=255)

                                                                                                                         aaaaTTTTAAATTTTTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GGTGATGTCT‑CAATTAATGAGAATGTTCCAAGTTATGACGTGAAATATAAAATGAGTAATAAAGATGAAAATGTTAAGCAAt                   >  1:1016202/1‑141 (MQ=255)

                                                                                                                         aaaaTTTTAAATTTTTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GGTGATGTCT‑CAATTAATGAGAATGTTCCAAGTTATGACGTGAAATATAAAATGAGTAATAAAGATGAAAATGTTAAGCAAt                   >  1:75057/1‑141 (MQ=255)

                                                                                                                          aaattttaaattttTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GGTGATGTCT‑CAATTAATGAGAATGTTCCAAGTTATGACGTGAAATATAAAATGAGTAATAAAGATGAAAATGTTAAGCAAtt                  <  1:338923/141‑1 (MQ=255)

                                                                                                                           aattttaaattttTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GGTGATGTCT‑CAATTAATGAGAATGTTCCAAGTTATGACGTGAAATATAAAATGAGTAATAAAGATGAAAATGTTAAGCAATTa                 <  2:690710/141‑1 (MQ=255)

                                                                                                                           aattttaaattttTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GGTGATGTCT‑CAATTAATGAGAATGTTCCAAGTTATGACGTGAAATATAAAATGAGTAATAAAGATGAAAATGTTAAGCAATTa                 <  2:590224/141‑1 (MQ=255)

                                                                                                                            attttaaattttTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GGTGATGTCT‑CAATTAATGAGAATGTTCCAAGTTATGACGTGAAATATAAAATGAGTAATAAAGATGAAAATGTTAAGCAATTaa                <  2:890177/141‑1 (MQ=255)

                                                                                                                            attttaaactttACAGTCAATATTAAAATAAAAAAAAAAAAAAAAACTACAGCAAT‑GGTGATGTCT‑CAATTAATGAGAATGTTCCAAGTTATGACGTGAAATATAAAATGAGTAATAAAGATGAAAATGTTAAGCAATTaa                <  2:890657/141‑1 (MQ=17)

                                                                                                                             ttttaaattttTCAGGCAATATGCAAATCCTAAAGAATTGAAAAACTACAGCAAT‑GGTGATGTCT‑CAATTAATGAGAATGTTCCAAGTTATGACGTGAAATATAAAATGAGTAATAAAGATGAAAATGTTAAGCAATTaag               <  1:963148/141‑1 (MQ=255)

                                                                                                                                      tttCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GGTGATGTCT‑CAATTAATGAGAATGTTCCAAGTTATGACGTGAAATATAAAATGAGTAATAAAGATGAAAATGTTAAGCAATTAAGAAGTCGTta      <  2:875294/141‑1 (MQ=255)

TCAGGACGTTTTATCGCAAGGATAAATTACCTGATAGAAAAAATTATAAAGTTGAAATGAAAAATAATAAAATCATTTTATTAGATAAAGTAGAAGATAAAAAGC‑TTAAACAAAAAATAGAAAATTTTAAATTTTTCAGTCAATATGCAAATCTTAAAGAATTGAAAAACTACAGCAAT‑GGTGATGTCT‑CAATTAATGAGAATGTTCCAAGTTATGACGTGAAATATAAAATGAGTAATAAAGATGAAAATGTTAAGCAATTAAGAAGTCGTTATAAT  >  CP000730/466348‑466625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`