BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	2,442,876	T→C	4.4%	T164T (ACA→ACG)	USA300HOU_2305 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,442,876	0	T	C	4.4%	316.3 / 2.8	112	T164T (ACA→ACG)	USA300HOU_2305	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (64/41); new base C (2/3); total (66/46)
Fisher's exact test for biased strand distribution p-value = 3.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01

Only 100 of 113 total aligned reads displayed.

TTTGAAACACTTGTAAGAAAATATAATGAGTATTTACTACATAATGATATTTCAAATTAGAAAAAAGGAAGTTATGATTTAATGGCCTTGAGCCTATCATAACTTCCTTTTATCATTTTATTGTTGTGTTGATGTTTCGATAACGTGGTACATCTTATCAAACATCAATTCGAAACCATGCAC‑CATGGCATCATGATATTCTTTTTTCTTTTGCTTGTATTCTAAATTAGTAAATCGTCTTTCTTTTTCAACTAATGAACGATAATAAAATAG  >  CP000730/2442744‑2443016

tttGAAACACTTGTAAGAAAATATAATGAGTATTTACTACATAATGATATTTCAAATTAGAAAAAAGGAAGTTATGATTTAATGGCCTTGAGCCTATCATAACTTCCTTTTATCATTTTATTGTTGTGTTGATGTTTCGAt                                                                                                                                       <  1:833440/141‑1 (MQ=255)

tttGAAACACTTGTAAGAAAATATAATGAGTATTTACTACATAATGATATTTCAAATTAGAAAAAAGGAAGTTATGATTTAATGGCCTTGAGCCTATCATAACTTCCTTTTATCATTTTATTGTTGTGTTGATGTTTCGAt                                                                                                                                       <  1:869464/141‑1 (MQ=255)

    aaaCACTTGTAAGAAAATATAATGAGTATTTACTACATAATGATATTTCAAATTAGAAAAAAGGAAGTTATGATTTAATGGCCTTGAGCCTATCATAACTTCCTTTTATCATTTTATTGTTGTGTTGATGTTTCGATAACg                                                                                                                                   >  2:543706/1‑141 (MQ=255)

    aaaCACTTGTAAGAAAATATAATGAGTATTTACTACATAATGATATTTCAAATTAGAAAAAAGGAAGTTATGATTTAATGGCCTTGAGCCTATCATAACTTCCTTTTATCATTTTATTGTCGTGTTGATGTTTCGATAACg                                                                                                                                   <  1:589646/141‑1 (MQ=255)

      acacTTGTAAGAAAATATAATGAGTATTTACTACATAATGATATTTCAAATTAGAAAAAAGGAAGTTATGATTTAATGGCCTTGAGCCTATCATAACTTCCTTTTATCATTTTATTGTTGTGTTGATGTTTCGATAACGTg                                                                                                                                 >  1:66662/1‑141 (MQ=255)

          ttGTAAGAAAATATAATGAGTATTTACTACATAATGATATTTCAAATTAGAAAAAAGGACGTTATGATTTAATGGCCTTGAGCCTATCATAACTTCCTTTTATCATTTTATTGTTTGGTTGATATTTCgtgaaatggctac                                                                                                                             >  2:632844/1‑129 (MQ=255)

            ttAAGAAAAAATAATGAGTATTTACTACATAATGATATTTCAAATTAGAAAAAAGGAAGTTATGATTTAATGGCCTTGAGCCTATCATAACTTCCTTTTATCATTTTATTGTTGTGTTGATGTTTCGATAACGTGGTACAt                                                                                                                           <  2:71455/140‑1 (MQ=255)

            gTAAGAAAATATAATGAGTATTTACTACATAATGATATTTCAAATTAGAAAAAAGGAAGTTATGATTTAATGGCCTTGAGCCTATCATAACTTCCTTTTATCATTTTGTTGTTGTGTTGATGTTTCGATAACGTGGTACAt                                                                                                                           >  2:187823/1‑141 (MQ=255)

            gTAAGAAAATATAATGAGTATTTACTACATAATGATATTTCAAATTAGAAAAAAGGAAGTTATGATTTAATGGCCTTGAGCCTATCATAACTTCCTTTTATCATTTTATTGTTGTGTTGATGTTTCGATAACGTGGTACAt                                                                                                                           >  1:495024/1‑141 (MQ=255)

            gTAAGAAAATATAATGAGTATTTACTACATAATGATATTTCAAATTAGAAAAAAGGAAGTTATGATTTAATGGCCTTGAGCCTATCATAACTTCCTTTTATCATTTTATTGTTGTGTTGATGTTTCGATAACGTGGTACAt                                                                                                                           <  2:576998/141‑1 (MQ=255)

            gTAAGAAAATATAATGAGTATTTACTACATAATGATATTTCAAATTAGAAAAAAGGAAGTTATGATTTAATGGCCTTGAGCCTATCATAACTTCCTTTTATCATTTTATTGTTGTGTTGATGTTTCGATAACGTGGTACAt                                                                                                                           >  1:371185/1‑141 (MQ=255)

            gTAAGAAAATATAATGAGTATTTACTACATAATGATATTTCAAATTAGAAAAAAGGAAGTTATGATTTAATGGCCTTGAGCCTATCATAAATTCCTTTTATCATTTTATTGTTGTGTTGATGTTTCGATAAAGTGGTAcct                                                                                                                           >  2:435591/1‑139 (MQ=255)

               aGAAAATATAATGAGTATTTACTACATAATGATATTTCAAATTAGAAAAAAGGAAGTTATGATTTAATGGCCTTGAGCCTATCATAACTTCCTTTTATCATTTTATTGTTGTGTTTATGTTTCGATAACGTGGTACATCtt                                                                                                                        >  1:199712/1‑141 (MQ=255)

               aGAAAATATAATGAGTATTTACTACATAATGATATTTCAAATTAGAAAAAAGGAAGTTATGATTTAATGGCCTTGAGCCTATCATAACTTCCTTTTATCATTTTATTGTTGTGTTGATGTTTCGATAACGTGGTACATCtt                                                                                                                        >  1:780119/1‑141 (MQ=255)

                gAAAATATAATGAGTATTTACTACATAATGATATTTCAAATTAGAAAAAAGGAAGTTATGATTTAATGGCCTTGAGTCTATCATAACTTCCTTTTATCATTTTATTGTTGTGTTGCCGTTTCGCTAACGTGGTACACCTTa                                                                                                                       >  1:905233/1‑141 (MQ=255)

                gAAAATATAATGAGTATTTACTACATAATGATATTTCAAATTAGAAAAAAGGAAGTTATGATTTAATGGCCTTGAGCCTATCATAACTTCCTTTTATCATTTTATTGTTGTGTTGACGTTTCGATAACGTGGTACATCTTa                                                                                                                       >  1:480706/1‑141 (MQ=255)

                    atatAATGAGTATTTACTACATAATGATATTTCAAATTAGAAAAAAGGAAGTTATGATTTAATGGCCTTGAGCCTATCATAACTTCCTTTTATCATTTTATTGTTGTGTTGATGTTTCGATAACGTGGTACATCTTTTCaa                                                                                                                   >  1:131144/1‑141 (MQ=255)

                    atatAATGAGTATTTACTACATAATGATATTTCAAATTAGAAAAAAGGAAGTTATGATTTAATGGCCTTGAGCCTATCATAACTTCCTTTTATCATTTTATTGTTGTGTTGATGTTTCGATAACGTGGTACATCTTATCaa                                                                                                                   >  2:439050/1‑141 (MQ=255)

                    atatAATGAGTATTTACTACATAATGATATTTCAAATTAGAAAAAAGGAAGTTATGATTTAATGGCCTTGAGCCTATCATAACTTCCTTTTATCATTTTATTGTTGTGTTGATGTTTCGATAACGTGGTACATCTTATCaa                                                                                                                   <  1:916370/141‑1 (MQ=255)

                     tataATGAGTATTTACTACATAATGATATTTCAAATTAGAAAAAAGGAAGTTATGATTTAATGGCCTTGAGCCTATCATAACTTCCTTTTATCATTGTATTGTTGTGTTGATGTTTCGATAACGTGGTACATCTTATCaaa                                                                                                                  >  2:484586/1‑141 (MQ=255)

                      ataATGAGTATTTACTACATAATGATATTTCAAATTAGAAAAAAGGAAGTTATGATTTAATGGCCTTGAGCCTATCATAACTTCCTTTTATCATTTTATTGTTGTGTTGATGTTTCGATAACGTGGTACATCTTATCAAAc                                                                                                                 >  1:423202/1‑141 (MQ=255)

                       taATGAGTATTTACTACATAATGATATTTCAAATTAGAAAAAAGGAAGTTATGATTTAATGGCCTTGAGCCTATCATAACTTCCTTTTATAATTTTATTGTTGTGTTGATGTTTCGATGACGTGGTACATCTTATCAAACa                                                                                                                >  2:874199/1‑141 (MQ=255)

                        aaTGAGTATTTACTACATAATGATATTTCAAATTAGAAAAAAGGAAGTTATGATTTAATGGCCTTGAGCCTATCATAACTTCCTTTTATCATTTTATTGTTGTGTTGATGTTTCGTTAACGTGGTACATCTTATCAAACAt                                                                                                               >  2:769249/1‑141 (MQ=255)

                        aaTGAGTATTTACTACATAATGATATTTCAAATTAGAAAAAAGGAAGTTATGATTTAATGGCCTTGAGCCTATCATAACTTCCTTTTATCATTTTATTGTTGTGTTGATGTTTCGATAACGTGGTACATCTTATCAAACAt                                                                                                               >  2:313042/1‑141 (MQ=255)

                         aTGAGTATTTATTACATAATGATATTTCAAATTAGAAGAAAAGAAGTTATGATTTAATGGCCTTGAGCCTATCATAACTTCCGTTTATCATTTTATTGTTGTGTTGATGTTTCGATAACGTGGTACATCTTTTCAAAAATc                                                                                                              >  2:620483/1‑141 (MQ=255)

                         aTGAGTATTTACTACATCATGATAATTCAAATTAGAAAAAAGGAAGTTATGATTTAATGGCCTTGAGCCTATCATAACTTCCTTTTATCATTTTATTGTTGTGTTGATGTTTCGATAACGTGGTACATCTTATCAAACATc                                                                                                              >  2:203802/1‑141 (MQ=255)

                         aTGAGTATTTACTACATAATGATATTTCAAATTAGAAAAAAGGAAGTTATGATTTAATGGCCTTGAGCCTATCATAACTTCCTTTTATCATTTTATTGTTGTGTTGATGTTTCGATAACGTGGTACATCTTTTTATACAtt                                                                                                              >  2:701471/1‑140 (MQ=255)

                         aTGAGTATTTACTACATAATGATATTTCAAATTAGAAAAAAGGAAGTTATGATTTAATGGCCTTGAGCCTATCATAACTTCCTTTTATCATTTTATTGTTGTGTTGATGTTTCGATAACGTGGTACATCTTATCAAACAta                                                                                                              >  2:362613/1‑140 (MQ=255)

                         aTGAGTATTTACTACATAATGATATTTCAAATTAGAAAAAAGGAAGTTATGATTTAATGGCCTTGAGCCTATCATAACTTCCTTTTATCATTTTATTGTTGTGTTGATGTTTCGATAACGTGGTACATCTTATCAAACATc                                                                                                              >  2:292577/1‑141 (MQ=255)

                         aTGAGTATTTACTACATAATGATATTTCAAATTAGAAAAAAGGAAGTTATGATTTAATGGCCTTGAGCCTATCATAACTTCCTTTTATCATTTTATTGTTGTGTTGATGTTTCGATAACGTGGTACATCTTATCAAACATc                                                                                                              >  2:507611/1‑141 (MQ=255)

                         aTGAGTATTTACTACATAATGATATTTCAAATTAGAAAAAAGGAAGTTATGATTTAATGGCCTTGAGCCTATCATAACTTCCTTTTATCATTTTATTGTTGTGTTGATGTTTCGATAACGTGGTACATCTTATCAAACATc                                                                                                              <  1:21859/141‑1 (MQ=255)

                         aTGAGTATTTACTACATAATGATATTTCAAATTAGAAAAAAGGAAGTTAGGATTTACTGGCCTTGAGACAATCATAACGAACTGTTATAATGTTATTGTTGTGTTGATGTTTCGATCACGTGTTACATATTATCAatcatc                                                                                                              >  2:114088/1‑141 (MQ=255)

                         aTGAGTATTTACTACATAATGATATTTCAAATTAGAAAAAAAAAAGTTATGATTTAATGACCTTGAGCCTATCATAACTTCCTTTTATAATTTTATTGTTGTGTTGATGTTTCGATAACGTGTTACATCTTTTCAAGCAta                                                                                                              >  2:154345/1‑140 (MQ=255)

                               ttgtactacATAATGTTATTTTAAATTAGAAAAAAGGAGGTTATGTTTTAATGGCCTTGAGCCTATCATAACTTCCTTTTATCATTTTAGTGTTGTGTTGATGTTTGGATAACGTGGTACATCTTATCAAACATCAATTCg                                                                                                        <  2:799871/138‑1 (MQ=255)

                               aTTTACTACATAATGCTATCTCAAACTAGAAAAAAGGAAGTTATGATTTAATGGCCTTGAGCCTATCATAACTTCCTTTTATCATTTTATTGTTGTGTTGATGTTTCGATAACGTGGTACATCTTATCAAACATCAATTCg                                                                                                        <  2:234998/141‑1 (MQ=255)

                                    ctacATAATGATATTTCAAATTAGAAAAAAGGAAGTTATGATTTAATGGCCTTGAGCCTATCATAACTTCCTTTTATCATTTTATTGTTGTGTTGATGTTTCGATAACGTGGTACATCTTATCAAACATCAATTCGAAAcc                                                                                                   >  1:658848/1‑141 (MQ=255)

                                    ctacATAATGATATTTCAAATTAGAAAAAAGGAAGTTATGATTTAATGGCCTTGAGCCTATCATAACTTCCTTTTATCATTTTATTGTTGTGTTGATGTTTCGATAACGTGGTACATCTTATCAAACATCAATTCGAAAcc                                                                                                   >  1:789782/1‑141 (MQ=255)

                                    ctacATAATGATATTTCAAATTAGAAAAAAGGAAGTTATGATTTAATGGCCTTGAGCCTATCATAACTTCCTTTTATCATTTTATTGTTGTGTTGATGTTTCGATAACGTGGTACATCTTATCAAACATCAATTCGAAAcc                                                                                                   >  1:269118/1‑141 (MQ=255)

                                       cATAATGATATTTCAAATTAGAAAAAAGGAAGTTATGATTTAATGGCATTGAGCCTATCATAACTTCCTTTTATCATTTTATTGTTGTGTAGATGTTTCGATAACGTGGTACTTCTTATCAAACATCAATTCGAAACCATg                                                                                                >  2:615598/1‑141 (MQ=255)

                                            tGATATTTCAAATTAGAAAAAAGGAAGTTATGATTTAATGGCCTTGAGCCTATCATAACTTCCTTTTATCATTTTATTGTTGTGTTGATGTTTCGATAACGTGGTACATCTTATCAAACATCAATTCGAAACCATGCAC‑Ca                                                                                          >  2:395835/1‑141 (MQ=255)

                                                atTTCAAATTAGAAAAAAGGAAGTTATGATTTAATGGCCTTGAGCCTATCATCACTTCCTTTTATCATTTTATTGTTGTGTTGATGTTTCGATAACGTGGTACATCTTATCAAACATCAATTCGAAACCATGCAC‑CATGGc                                                                                      >  1:28212/1‑141 (MQ=255)

                                                atTTCAAATTAGAAAAAAGGAAGTTATGATTTAATGGCCTTGAGCCTATCATAACTTCCTTTTATCATTTTATTGTTGTGTTGATGTTTCGATAACGTGGTACATCTTATCAAACATCAATTCGAAACCATGCAC‑CATGGc                                                                                      >  1:111617/1‑141 (MQ=255)

                                                atTTCAAATTAGAAAAAAGGAAGTTATGATTTAATGGCCTTGAGCCTATCATAACTTCCTTTTATCATTTTATTGTTGTGTTGATGTTTCGATAACGTGGTACATCTTATCAAACATCAATTCGAAACCATGCAC‑CATGGc                                                                                      >  2:736776/1‑141 (MQ=255)

                                                atTTCAAATTAGAAAAAAGGAAGTTATGATTTAATGGCCTTGAGCCTATCATAACTTCCTTTTATCATTTTATTGTTGTGTTGATGTTTCGATAACGTGGTACATCTTATCAAACATCAATTCGAAACCATGCAC‑CATGGc                                                                                      <  2:69736/141‑1 (MQ=255)

                                                  ttCAAATTAGAAAAAAGGAAGTTATGATTTAGTGGCCTTGAGCCTATCATAACTTCCTTTTATCATTTTATTGTTGTGTTGATGTTTCGATAACGTGGTACATCTTATCAAACATCAATTCGAAACCATGCAC‑CATGGcat                                                                                    <  1:617817/141‑1 (MQ=255)

                                                  ttCAAATTAGAAAAAAGGAAGTTATGATTTAATGGCCTTGAGCCTATCATAACTTCCTTTTATCATTTTATTGTTGTGTTGATGTTTCGATAACGTGGTACATCTTATCAAACATCAATTCGAAACCATGCAC‑CATGGcat                                                                                    <  1:507872/141‑1 (MQ=255)

                                                          aGAAAAAAGGAAGTTATGATTTAATGGCCTTGAGCCTATCATAACTTCCTTTTATCATTTTATTGTTGTGTTGATGTTTCGATAACGTGGTACATCTTATCAAACATCAATTCGAAACCATGCAA‑CATGGCATCATGatat                                                                            >  2:307372/1‑141 (MQ=255)

                                                            aaaaaaGGAAGTTATGATTTAATGGCCTTGAGCCTATCATAACTTCCTTTTATCATTTTATTGTTGTGTTGATGTTGCGATAACGTGGTACATCTTATCAAACATCAATTCGAAACCATGCAC‑CATGGCATCATGATAttc                                                                          <  2:147833/141‑1 (MQ=255)

                                                              aaaaGGAAGTTATGATTTAATGGCCTTGAGCCTATCATAACTTCCTTTTATCCTTTTATTGTTGTGTTGATGTTTCGATAACGTGGTACATCTTATCAAACATCCATTCGAAACCATGCAC‑CCTGGCCTCACAATAttctt                                                                        >  2:748833/1‑141 (MQ=255)

                                                              aaaaGGAAGTTATGATTTAATGGCCTTGAGCCTATCATAACTTCCTTTTATCATTTTATTGTTGTGTTGATGTTTCGATAACGTGGTACATCTTATCAAACATCAATTCGAAACCATGCAC‑CATGGCATCATGATAttctt                                                                        >  2:303921/1‑141 (MQ=255)

                                                              aaaaGGAAGTTATGATTTAATGGCCTTGAGCCTATCATAACTTCCTTTTATCATTTTATTGTTGTGTTGATGTTTCGATAACGTGGTACATCTTATCAAACATCAATTCGAAACCATGCAC‑CATGGCATCATGAAAttctt                                                                        >  2:447143/1‑141 (MQ=255)

                                                                aatgAATTTATTATTTAATGTCCTTGAGCCTATCATAACTTCCTTTTATCATTTTATTGTTGTGTTGATGTTTCGATAACGTGGTACATCTTATCAAACATCAATTCGAAACCATGCAC‑CATGGCATCATGATAttctttt                                                                      <  2:862757/138‑1 (MQ=255)

                                                                aaGGAAGTTATGATTTAATGGCCTTGAGCCTATCATAACTTCCTTTTATCATTTTATTGTTGTGTTGATGTTTCGATAACGTGGTACATCTTATCAAACATCAATTCGAAACCATGCAC‑CATGGCATCATGATAttctttt                                                                      <  2:554097/141‑1 (MQ=255)

                                                                aaGGAAGTTATGATTTAATGGCCTTGAGCCTATCATAACTTCCTTTTATCATTTTATTGTTGTGTTGATGTTTCGATAACGTGGTACATCTTATCAAACATCAATTCGAAACCATGCAC‑CATGGCATCATGATAttctttt                                                                      <  1:899905/141‑1 (MQ=255)

                                                                    aaGTTATGATTTAATGGCCTTGAGCCTATCATAACTTCCTTTTATCATTTTATTGTTGTGTTGATGTTTCGATAACGTGGTACATCTTATCAAACATCAATTCGAAACCATGCAC‑CATGGCATCATGATAttcttttttct                                                                  <  1:835199/141‑1 (MQ=255)

                                                                    aaGTTATGATTTAATGGCCTTGAGCCTATCATAACTTCCTTTTATCATTTTATTGTTGTGTTGATGTTTCGATAACGTGGTACATCTTATCAAACATCAATTCGAAACCATGCAC‑CATGGCATCATGATAttcttttttct                                                                  <  1:323630/141‑1 (MQ=255)

                                                                    aaGTTATGATTTAATGGCCTTGAGCCTATCATAACTTCCTTTTATCATTTTATTGTTGTGTTGATGTTTCGATAACGTGGTACATCTTATCAAACATCAATTCGAAACCATGCAC‑CATGGCATCATGATAttcttttttct                                                                  <  1:848345/141‑1 (MQ=255)

                                                                     atttATGATGTAATGGCCTTGAGCCTATCATAACTTCGCTTTATCATGTTACTGTTGTGTTGATGTTTCTACAACGTGGGACATCTTATCAAACATCAATTCGAAACCATGCAC‑CATGGCATCATGATAgtcttttttctt                                                                 <  2:169792/139‑1 (MQ=255)

                                                                     aGTTATGATTTAATGGCCTTGAGCCTATCATAACTTCCTTTTATCATTTTATTGTTGTGTTGATGTTTCGATAACGTGGTACATCTTATCAATCATCAATTCGAAACCATGCAC‑CATGGCATCATGATAttcttttttttt                                                                 >  2:454070/1‑141 (MQ=255)

                                                                     aGTTATGATTTAATGGCCTTGAGCCTATCATAACTTCCTTTTATCATTTTATTGTTGTGTTGATGTTTCGATAACGTGGTACATCTTATCAAACATCAATTCGAAACCATGCAC‑CATGGCATCATGATAttcttttttctt                                                                 <  2:283346/141‑1 (MQ=255)

                                                                     aGTTATGAGTTAATGGCCTTGAGCCTATCATAACTTCCTTTTATCATTTTATTGTTGTGTTGATGTTTCGATAACGTGGTACATCTTATCAAACATCAATTCGAAACCATGCAC‑CATGGCATCATGATAttcttttttctt                                                                 <  2:559387/141‑1 (MQ=255)

                                                                       ttATGATTTAATGGCCTTGAGCCTATCATAACTTCCTTTTATCATTTTATTGTTGTGTTGATGTTTCGATAACGTGGTACATCTTATCAAACATCAATTCGAAACCATGCAC‑CATGGCATCATGATAttcttttttctttt                                                               >  1:878386/1‑141 (MQ=255)

                                                                       ttATGATTTAATGGCCTTGAGCCTATCATAACTTCCTTTTATCATTTTATTGTTGTGTTGATGTTTCGATAACGTGGTACATCTTATCAAACATCAATTCGAAACCATGCAC‑CATGGCATCATGATAttcttttttctttt                                                               >  2:815309/1‑141 (MQ=255)

                                                                         aTGATTTAATGGCCTTGAGCCTATCATAACTTCCTTTTATCATTTTATTGTTGTGTTGATGTTTCGATAACGTGGTACATCTTATCAAACATCAATTCGAAACCATGCAC‑CACGGCAACATGCTATTCTTTTTTCTTTTGc                                                             >  2:396450/1‑141 (MQ=255)

                                                                          tGATTTAATGGCCTTGAGCCTATCATAACTTCCTTTTATCATTTTATTGTTGTGTTGATGTTTCGATAACGTGGTACATCTTATCAAACATCAATTCGAAACCATGCAC‑CATGGCATCATGATATTCTTTTTTCTTTTGCt                                                            >  1:394118/1‑141 (MQ=255)

                                                                            aTTTCATGGCCTTTAGCCTATCATAACTTCCTTTTATCATTTTATTGTTGTGTTGATGTTTCGATAACGTGGTACATCTTATCAAACATCAATTCGAAACCATGCAC‑CATGGCATCATGATATTCTTTTTTCTTTTGCTTg                                                          <  1:73317/141‑1 (MQ=255)

                                                                            aTTTAATGGCCTTGAGCCTATCATAACTTCCTTTTATCATTTTATTGTTGTGTTGATGTTTCGATAACGTGGTACATCTTATCAAACATCAATTCGAAACCATGCAC‑CATGGCATCATGATATTCTTTTTTCTTTTGCTTg                                                          <  2:479533/141‑1 (MQ=255)

                                                                            aTTTAATGGCCTTGAGCCTATCATAACTTACTTTTATCATTTTATTGTTGTGTTGATGTTTCGATAACGTGGTACATCTTATCAAACATCAATTCGAAACCATGCAC‑CATGGCATCATGATATTCTTTTTTCTTTTGCTTg                                                          >  2:474702/1‑141 (MQ=255)

                                                                               ttaTGGCCTTGGGCCTATCATAACTTCCTTTTATCATTTTATTGTTGTGTTGATGTTTCGATAACGTGGTACATCTTATCAAACATCAATTCGAAACCATGCAC‑CATGGCATCATGATATTCTTTTTTCTTTTGCTTGTAt                                                       <  2:303813/139‑1 (MQ=255)

                                                                               tAATGGCCTTGATCCTATCATAACTTCCTTTCATCATTTTATTGTTGTGTTGATGTTTCGATAACGTGGTACATCTTATCAAACATCAATTCGAAACCATGCAC‑CATGGCATCATGATATTCTTTTTTCTTTTGCTTGTAt                                                       <  1:150491/141‑1 (MQ=255)

                                                                               tAATGGCCTTGAGCCTATCATAACTTCCTTTTATCATTTTATTGTTGTGTTGATGTTTCGATAACGTGGTACATCTTATCAAACATCAATTCGAAACCATGCAC‑CATGGCATCATGATATTCTTTTTTCTTTTGCTTGTAt                                                       <  1:593360/141‑1 (MQ=255)

                                                                               tAATGGCCTTGAGCCTATCATAACTTCCTTTTATCATTTTATTGTTGTGTTGATGTTTCGATAACGTGGTACATCTTATCAAACATCAATTCGAAACCATGCAC‑CATGGCATCATGATATTCTTTTTTCTTTTGCTTGTAt                                                       <  2:466872/141‑1 (MQ=255)

                                                                               tAATGGCCTTGAGCCTATCATAACTTCCTTTTATCATTTTATTGTTGTGTTGATGTTTCGATAACGTGGTACATCTTATCAAACATCAATTCGAAACCATGCAC‑CATGGCATCATGATATTCTTATTTCTTTTGCTTGTAt                                                       <  1:469733/141‑1 (MQ=255)

                                                                                 aTGGCCTTGAGCCTATCATAACTTCCTTTTATCATTTTATTATTGTCTTCATTGGTGGTGCAGTCGTTATATCTTATCAAACATCCATTCGAAACCATGCAC‑CATGGCATCATGATATTCTTTTTTCTTTTGCTTGTATTc                                                     <  2:905559/141‑1 (MQ=255)

                                                                                   ggCCTTGAGCCTATCATAACTTCCTTTTATCATTTTATTGTTGTGTTGATGTTTCGATAACGTGGTACATCTTATCAAACATCAATTCGAAACCATGCAC‑CCTGGCATCATGATATTCTTTTTTTTTTTTCTTGTATTCTa                                                   >  2:575974/1‑141 (MQ=255)

                                                                                          aGCCTATCATAACTTCCTTTTATCATTTTATTGTTGTGTTGATGTTTCGATAACGTGGTACATCTTATCAAACATCAATTCGACACAACGCGC‑AATGGCATCTGGTTATTCTTTTTTCTTTTGTTTGTATTCTAAATTGGt                                            >  2:802956/1‑141 (MQ=255)

                                                                                           gCCTATCATAACTTCCTTTTATCATTTTATTGTTGTGTTGATGTTTCGATAACGTGGTACATCTTATCAAACATCAATTCGAAACCATGCAC‑CATGGCATCATGATATTCTTTTTTCTTTTGCTTGTATTCTAAATTAGTa                                           <  2:418604/141‑1 (MQ=255)

                                                                                              tATCATAACTTCCTTTTATCATTTTATTGTTGTGTTGATGTTTCGATAACGTGGTACATCTTATCAAACATCAATTCGAAACCATGCAC‑CATGGCATCATGATATTCTTTTTTCTTTTGCTTGTATTCTAAATTAGTAAAt                                        >  1:462065/1‑141 (MQ=255)

                                                                                               aTCATAACTTCCTTTTATCATTTTATTGTTGTGTTGATGTTTCGATAACGTGGTACATCTTATCAAACCTCAATTCGAAACCATGCAC‑CATGGCATCATGATATTCTTTTTTCTTTTGCTTGTTATCTAAATTAGTAAATc                                       >  2:39068/1‑141 (MQ=255)

                                                                                                  aTAACTTCCTTTTATCATTTTATTGTTGTGTTGATGTTTCGATAACGTGGTACATCTTATCAAACATCAATTCGAAACCATGCAC‑CATGGCATCATGATATTCTTTTTTCTTTTGCTTGTATTATAAATTAGTAAATCGtc                                    >  1:119833/1‑141 (MQ=255)

                                                                                                      tttCCTTTTATCATTTTATTGTTGTTTTGACGTTTCGATAACGTGGTACATCTTATCAAACATCAATTCGAAACCATTCAC‑CATGGCATCATGATATTCTTTTTTCTTTTGCTTTTATTCTAAATTAGTAAATCGtctttc                                <  2:587273/140‑1 (MQ=255)

                                                                                                       ttCCTTTTATCATTTTATTGTTGTGTTGATGTTTCGATAACGTGGTACATCTTATCAAACATCAATTCGAAACCATGCAC‑CATGGCATCATGATATCCTTTTTTCTTTTGCTTGTATTCTAAATTAGTAAATCGtctttct                               <  1:515453/141‑1 (MQ=255)

                                                                                                          cTTTTATCATTTTATTGTTGTGTTGATGTTTCGATAACGTGGTACATCTTATCAAACATCAATTCGAAACCATGCAC‑CATGGCATCATGATATTCTTTTTTCTTTTGCTTGTATTCTAAATTAGTTAATCGTCTTTCtttt                            <  1:474702/141‑1 (MQ=255)

                                                                                                          cTTTTATCATTTTATTGTTGTGTTGACGTTTCGATCACGTGGTCCATGTTCTAGCTCGTCAATTCCAAACCATGCAC‑CATGGCATCATGATATTCTTTTTTCTTTTGCTTGTATTCTAAATTAGTAAATCGTCTTTCtttt                            <  2:905233/141‑1 (MQ=255)

                                                                                                            tttATCATTTTATGGTTGTGTTGATGTTTCGATAACGTGGTACATCTTATCAAACATCAATTCGAAACCATGCAC‑CATGGCATCATGATATTCTTTTTTCTTTTGCTTGTATTCTAAATTAGTAAATCGTCTTTCTTTTTc                          >  2:456831/1‑141 (MQ=255)

                                                                                                             ttATCATTTTATTGTTGTGTTGATGTTTCGATAACGTGGTACATCTTATCAAACATCAATTCGAAACCATGCAC‑CATGGCATCATGATATTCTTTTTTCTTTTGCTTGTATTCTAAATTAGTAAATCGTCTTTCTTTTTCa                         >  2:332823/1‑141 (MQ=255)

                                                                                                              tATCATTTTATTGTTGTGTTGATGTTTCGATAACGTGGTACATCTTATCAAACATCAATTCGAAACCATGCAC‑CATGGCATCATGATATTCTTTTTTCTTTTGCTTGTATTCTAAATTAGTAAATCGTCTTTCTTTTTCaa                        >  2:563461/1‑141 (MQ=255)

                                                                                                               aTCATTTTATTGTTGTGTTGATGTTTCGATAACGTGGTACATCTTATCAAACATCAATTCGAAACCATGCAC‑CATGGCATCATGATATTCTTTTTTCTTTTGCTTGTATTCTAAATTAGTAAATCGTCTTTCTTTTTCAAc                       >  1:527848/1‑141 (MQ=255)

                                                                                                                 cATTTTATTGTTGTGTTGAGGTTTCGATAACGTGGTACATCTGATCAAACCTCAATTCGAAACCATGCAC‑CATGGCATCATGATATTCTTTTTTCTTTTGCTTGTATTCTAAATTAGTAAATCGTCTTTCTTTTTCAACTa                     <  2:779894/141‑1 (MQ=255)

                                                                                                                            tgtgttgATGTTTCGATAACGTGGTACATCTTATCAAACATCAATTCGAAACCATGCAC‑CATGGCATCATGATATTCTTTTTTCTTTTGCTTGTATTCTAAATTAGTAAATTGTTTTTCTTTTTCAACTATTTAATGataa          >  2:507402/1‑141 (MQ=255)

                                                                                                                            tgtgttgATGTTTCGATAACGTGGTACATCTTATCAAACATCAATTCGAAACCATGCAC‑CATGGCATCATGATATTCTTTTTTCTTTTGCTTGTATTCTAAATTAGTAAATCGTCTTTCTTTTTCAACTAATGAACGataa          >  1:511176/1‑141 (MQ=255)

                                                                                                                            tgtgttgAGGTTTCGATAACGTGGTACATCTTATCAAACATCAATTCGAAACCATGCAC‑CATGGCATCATGATATTCTTTTTTCTTTTGCTTGTATTCTAAATTAGTAAATCGTCTTTCTTTTTCAACTAATGAACGataa          <  2:423202/141‑1 (MQ=255)

                                                                                                                             gtgttgATGTTTCGATAACGTGGTACATCTTATCAAACATCAATTCGAAACCATGCAC‑CATGGCATCATGATATTCTTTTTTCTTTTGCTTGTATTCTAAATTAGTAATTCGTCTTTCTTTTTCATCTAATTAACGataat         >  1:208226/1‑141 (MQ=255)

                                                                                                                               tttgATGTTTCGATAACGTGGTACATCTTATCAAACATCAATTCGAAACCATGCAC‑CATGGCATCATGATATTCTTTTTTCTTTTGCTTGTATTCTAAATTAGTAAATCGTCTTTCTTTTTCAACTAATGAACGAtaataa       <  2:751929/140‑1 (MQ=255)

                                                                                                                               gttgATGTTTCGATAACGTGGTACATCTTATCAAACATCAATTCGAAACCATGCAC‑CATGGCATCATGATATTCTTTTTTCTTTTGCTTGTATTCTAAATTAGTAAATCGTCTTTCTTTTTCAATTAATGAACTAtcataa       >  2:596450/1‑141 (MQ=255)

                                                                                                                               gttgATGTTTCGATAACGTGGTACATCTTATCAAACATCAATTCGAAACCATGCAC‑CATGGCATCATGATATTCTTTTTTCTTTTGCTTGTATTCTAAATTAGTAAATCGTCTTTCTTTTTCAACTAATGAACGAtaataa       <  2:578998/141‑1 (MQ=255)

                                                                                                                               gttgATGTTTCGATAACGTGGTACATCTTATCAAACATCAATTCGAAACCATGCAC‑CATGGCATCATGATATTCTTTTTTCTTTTGCTTGTATTCTAAATTAGTAAATCGTCTTTCTTTTTCAACTAATGAACGAtaataa       <  1:115813/141‑1 (MQ=255)

                                                                                                                               gttgATGTTTCGATAACGTGGTACATCTTATCAAACATCAATTCGAAACCATGCAC‑CATGGCATCATGATATTCTTTTTTCTTTTGCTTGTATTCTAAATTAGTAAATCGTCTTTCTTTTTCAACTAATGAACGAtaataa       <  1:592074/141‑1 (MQ=255)

                                                                                                                                  gATGTTTCGATAACGTGGTACATCTTATCAAACATCAATTCGAAACCCT‑CACACCTGGCATCATGATATTCTTTTTTCTTTTGCTTGTATTCTAAATTAGTAAATCGTCTTTCTTTTTCAACTAATGAACGATAATAAAAt    <  1:26914/141‑1 (MQ=255)

                                                                                                                                    tGTTTCGATAACGTGGTACATCTTATCAAACATCAATTCGAAACCATGCAC‑CATGGCATCATGATATTCTTTTTTCTTTTGCTTGTATTCTAAATTAGTAAATCGTCTTTCTTTTTCAACTAATGAACGATAATAAAATAg  <  1:543706/141‑1 (MQ=255)

TTTGAAACACTTGTAAGAAAATATAATGAGTATTTACTACATAATGATATTTCAAATTAGAAAAAAGGAAGTTATGATTTAATGGCCTTGAGCCTATCATAACTTCCTTTTATCATTTTATTGTTGTGTTGATGTTTCGATAACGTGGTACATCTTATCAAACATCAATTCGAAACCATGCAC‑CATGGCATCATGATATTCTTTTTTCTTTTGCTTGTATTCTAAATTAGTAAATCGTCTTTCTTTTTCAACTAATGAACGATAATAAAATAG  >  CP000730/2442744‑2443016

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`