BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,087	A→G	58.4%	C155C (TGT→TGC)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,087	0	A	G	58.4%	794.1 / inf	1367	C155C (TGT→TGC)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base A (274/292); new base G (410/380); total (691/676)
Fisher's exact test for biased strand distribution p-value = 2.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

Only 100 of 1369 total aligned reads displayed.

TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTAT  >  CP000730/35947‑36226

tttttATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGa                                                                                                                                             <  2:706363/141‑1 (MQ=40)

    tatataGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGACAGt                                                                                                                                         <  1:248940/141‑1 (MQ=255)

     atataGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGACAGTc                                                                                                                                        >  1:312208/1‑141 (MQ=255)

        taGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGCTCTTCCTCAATGAGG‑TTATTCAGATCTTTCGATGTACAATGGCAGTAAgg                                                                                                                                     >  1:658343/1‑141 (MQ=14)

          ttttgTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGACAGTCAGgtt                                                                                                                                   >  2:582029/5‑141 (MQ=255)

           agCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGACAGTCAGgttt                                                                                                                                  >  1:816181/1‑141 (MQ=40)

           agCGTAAATACACTCAATACCTTTTAGAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGATGGTGATCTTGCTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGGCAGTCAGgttt                                                                                                                                  <  1:577695/141‑1 (MQ=14)

              ggaaaTACACTCAATACCTTTTAAAGGATTCTTTGCTGTTTGGATACTTTGGTAACTTGTCTTTCTTACTTTAATAAGACGGTGATCTTTCTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaag                                                                                                                               <  2:256634/139‑1 (MQ=14)

                 aaTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGG‑TTATTCAGATCTTTCGATGTACAATGGCAGTAAGgtttaagttt                                                                                                                            >  2:791585/1‑141 (MQ=255)

                 aaTACACTCAATACCTTTTAAACTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACCTTAATATGACGGTGATCTTGCTCAATGAGG‑TTATTCAGATATTTCGATGCACAATGGCAGTCAGgtttaagttt                                                                                                                            <  1:706229/141‑1 (MQ=14)

                  aTACATTCAATACCTTTTAAAGTATTCGTTGCCGTATTGATACTTTGATATCTTGACTTTCTTACTTTAATATGACGGAGCTCTTGCTCAATGAGG‑TTGTTGATATATGTCGATGTACAATGACAGTCAGgtttgagtttt                                                                                                                           >  2:370526/1‑140 (MQ=14)

                  aTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagttta                                                                                                                           <  2:873074/141‑1 (MQ=255)

                  aTACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagttta                                                                                                                           >  1:508873/1‑141 (MQ=255)

                   tACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGGCAGTCAGgtttaagtttaa                                                                                                                          >  1:772648/1‑141 (MQ=255)

                     cactCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTaaaa                                                                                                                        <  2:220791/138‑1 (MQ=255)

                        ccAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGCCAGTCAGGTTTAAGTTTAAAAGCt                                                                                                                     <  2:202453/140‑1 (MQ=255)

                            tACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGGCAGTCAGgt                                                                                                                                    >  2:278946/1‑122 (MQ=255)

                            tACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTGACTTTAATATGACGATGCTCTTTCTCCGTGCGGCTCAGGCGGAT‑TTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTat                                                                                                                 >  1:409414/1‑140 (MQ=14)

                            tACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTaa                                                                                                                 >  1:831062/1‑141 (MQ=255)

                             aCCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAAt                                                                                                                >  1:758916/1‑141 (MQ=255)

                             aCCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAAt                                                                                                                <  1:63172/141‑1 (MQ=255)

                                 tttAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACt                                                                                                            >  1:400754/1‑141 (MQ=255)

                                   tAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACttt                                                                                                          <  1:920626/141‑1 (MQ=255)

                                    acaGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTa                                                                                                         <  1:273783/139‑1 (MQ=255)

                                     aaGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAg                                                                                                        >  1:722140/1‑141 (MQ=255)

                                        tATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCa                                                                                                     <  2:293202/141‑1 (MQ=255)

                                         aTTCTTTGCTGTATGGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGGCAGTAAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCAt                                                                                                    >  1:522604/1‑141 (MQ=255)

                                         aTTCTTTGCCGTATTGATAATTTGATATCTTGTCTTTCTTACGTTAAGATGACGGGGATGTTGCTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCAt                                                                                                    >  2:318773/1‑141 (MQ=11)

                                              ttGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTa                                                                                               >  2:30814/1‑141 (MQ=255)

                                                 cTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCt                                                                                            >  2:392359/1‑141 (MQ=255)

                                                   gTATTGATACTTTGATATCTTGTTTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGCCAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTc                                                                                          >  1:457542/1‑141 (MQ=14)

                                                   gTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTc                                                                                          <  2:161252/141‑1 (MQ=255)

                                                      ttGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGtt                                                                                       >  2:91059/1‑141 (MQ=255)

                                                       ttaTACTTTGATACCTTGACTTTCTTACTTTAATTTGACTGTTATCTTGCTCAATGAGG‑ATATTCAGATATTTCTATGTACAAGGGCAGGCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTg                                                                                      <  1:785939/139‑1 (MQ=11)

                                                        gATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGa                                                                                     <  2:515272/141‑1 (MQ=255)

                                                         aTACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGaa                                                                                    >  2:52985/1‑141 (MQ=255)

                                                          tACTTTGATATCTTGTCTTTCTTACTTTAATATTACGGTGATCTTGCTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAg                                                                                   <  1:861906/141‑1 (MQ=255)

                                                          tACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAg                                                                                   <  2:186953/141‑1 (MQ=255)

                                                          tACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAg                                                                                   >  2:721371/1‑141 (MQ=255)

                                                           aCTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAgg                                                                                  <  2:543186/141‑1 (MQ=255)

                                                             tttGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTg                                                                                <  1:194446/141‑1 (MQ=255)

                                                               tGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAACAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGcc                                                                              >  2:176268/1‑141 (MQ=255)

                                                               tGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGcc                                                                              <  1:105028/141‑1 (MQ=255)

                                                               tGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGcc                                                                              >  1:5973/1‑141 (MQ=255)

                                                                gATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAg                                                                                   <  2:330731/135‑1 (MQ=38)

                                                                gATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAg                                                                                   >  1:330731/1‑135 (MQ=39)

                                                                 atatcTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTg                                                                            >  1:12987/5‑141 (MQ=255)

                                                                 aTAGCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTTCATGAGG‑TTGTTCAGATATTTCGATGTACAGTGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTGTCCATTGCTGCCTTCGTTGAAGGTGCCtc                                                                            >  2:404336/1‑140 (MQ=11)

                                                                      ttGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAGGTTTAAAAGCGTTAATTATTTTAGCCCTCCCCGCCTTCGTTGAAGGTGCCTGATCtt                                                                       >  2:614792/1‑140 (MQ=14)

                                                                      ttGTCTTTCTTACTGTAATATGACGGTGATCTTGCTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTg                                                                       >  1:491945/1‑141 (MQ=255)

                                                                       tGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGt                                                                      <  2:558346/141‑1 (MQ=255)

                                                                        gTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGCAGGTGCCTGATCTGTa                                                                     <  1:561162/141‑1 (MQ=255)

                                                                         tctttcttACTTTAATATGACGGTGATCTTGCTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTaa                                                                    >  1:90284/1‑141 (MQ=255)

                                                                            ttcttACTCTAATATGACAGTGATCTTGCCCAATGAGG‑TTATTCAGAAATTTCGATGTACAATGGCAGTCAGCTTTAAGTTCAAAAGCATTAATTACTTTAACCATTGCTACCTTCGCTGAAGGTGCCTGATCTGTAATTa                                                                 <  2:447911/141‑1 (MQ=11)

                                                                                 aCTTTAATATGACGGTGATCTTGCTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCttt                                                            >  2:603104/1‑141 (MQ=255)

                                                                                 aCTTTAATATGACGGTGATCTTGCTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCttt                                                            <  2:829088/141‑1 (MQ=255)

                                                                                   tttAATATGACGGTGATCTTGCTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTg                                                          <  1:284801/141‑1 (MQ=25)

                                                                                    ttAATATGACGGTGATCTTGCTCAATGAGG‑TTATTCCGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGa                                                         <  2:471879/141‑1 (MQ=255)

                                                                                    ttAATATGACGGTGATCTTGCTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTgg                                                         >  2:747110/1‑140 (MQ=255)

                                                                                    ttAATATGACGGTGAACTAGATCAATGACA‑TTATTCAGATATTTCGATTTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGa                                                         >  2:417842/1‑141 (MQ=11)

                                                                                        tttGACGGTGATCTTGCTCAATGAGG‑TTATTCAGATATTTCGATGTACCATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGtt                                                     <  2:930516/139‑1 (MQ=255)

                                                                                        tatGACGGTGATCTTGCTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAggct                                                     <  1:846738/141‑3 (MQ=255)

                                                                                        aatGACGGTGATCGTGCTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAggct                                                     <  1:93489/140‑3 (MQ=11)

                                                                                          tGACGGTGATCTTGCTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTa                                                   <  1:234261/141‑1 (MQ=11)

                                                                                           gACGGTGATCTTGCTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAc                                                  <  1:356396/141‑1 (MQ=21)

                                                                                            ccGGTGATCTTGCTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAcc                                                 <  1:147725/140‑1 (MQ=19)

                                                                                            aCGGTGATCTTGCTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAcc                                                 <  1:386545/141‑1 (MQ=25)

                                                                                             cGGTGATCTTGCTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTTAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCa                                                >  2:633671/1‑141 (MQ=255)

                                                                                               gTGATCTTGCTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCaaa                                              <  2:849776/141‑1 (MQ=12)

                                                                                                 gATCTTGCTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTACTACCTTCGTTGAAGGTGCCTGATCCGTAATTATCTTTTGAGGTTTACCAAAtt                                            <  1:609989/141‑1 (MQ=11)

                                                                                                  aTCTTGCTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTg                                           <  1:797513/141‑1 (MQ=12)

                                                                                                  aTCTTGCTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTg                                           <  2:138341/141‑1 (MQ=11)

                                                                                                   tCTTGCTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGt                                          <  1:877803/141‑1 (MQ=11)

                                                                                                    cTTGCTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAGATTGtt                                         >  1:466087/1‑141 (MQ=255)

                                                                                                    cTTGCTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGtt                                         >  2:481569/1‑141 (MQ=32)

                                                                                                       gCTCAATGTGG‑TTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTaa                                      <  1:477998/141‑1 (MQ=255)

                                                                                                        cTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAAt                                     <  1:682973/141‑1 (MQ=17)

                                                                                                         tCAATGAGG‑TTCTTCAGATATTTCGATTTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATg                                    <  2:117222/141‑1 (MQ=255)

                                                                                                         tCAATGAGG‑TTATTCAGATACTTCGATTTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTTCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATg                                    <  2:500180/141‑1 (MQ=11)

                                                                                                          cAATGAGG‑TTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATga                                   <  1:743604/141‑1 (MQ=12)

                                                                                                           aaTGAGG‑TTATTGAGATATTTCGTTGTAGATTGGCAGTTAGGTGTAAGGTTAAAAGCTCTGATTATTTTGGTCATCGCTGCCTTCGTCGAAGGTGGCTGATCTGTAAATACCTTTTGAGGTTTACCAAATTGGTTAATgag                                  <  2:356241/141‑1 (MQ=2)

                                                                                                               ggg‑TTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGt                              <  2:803815/140‑1 (MQ=11)

                                                                                                                gg‑TTATTCCAGTGTTTTGATGTACCAAGGCATTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGtt                             <  2:235615/141‑1 (MQ=11)

                                                                                                                gg‑TTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGtt                             >  1:29969/1‑141 (MQ=12)

                                                                                                                gg‑TTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGtt                             <  2:765864/141‑1 (MQ=17)

                                                                                                                   ttATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGCTCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTg                           <  1:735698/141‑1 (MQ=11)

                                                                                                                   ttATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTg                           <  2:892315/141‑1 (MQ=255)

                                                                                                                    tATTCAGTTATTTCGATGTATAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATCACTTGAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGa                          <  1:163180/141‑1 (MQ=11)

                                                                                                                    tATTCAGATATTTCGATGTACAATGGCAGTCATGTTTAAGTTTAAAAGCTTTAATTCCATTATCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGa                          <  1:448796/141‑1 (MQ=11)

                                                                                                                            tatTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCa                  <  1:542551/141‑1 (MQ=11)

                                                                                                                            tatTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCa                  <  1:369418/141‑1 (MQ=32)

                                                                                                                                tCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATg              >  2:65713/1‑141 (MQ=32)

                                                                                                                                  gATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGCGACGTTTGATAAACGCATATGCt            >  1:43154/1‑141 (MQ=17)

                                                                                                                                   aTGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTg           <  1:67205/141‑1 (MQ=11)

                                                                                                                                       aCAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATg       <  1:641665/141‑1 (MQ=17)

                                                                                                                                       aCAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATg       >  1:171256/1‑141 (MQ=32)

                                                                                                                                        cAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATTTCTGAATAGCGGTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATgt      >  1:217975/1‑140 (MQ=11)

                                                                                                                                        cAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGa      >  1:98604/1‑141 (MQ=17)

                                                                                                                                           tGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTa   <  2:736455/141‑1 (MQ=11)

                                                                                                                                            gACAGCCATGTTTCAGTTTAAAAGTTTTAATTAGTTTAGCCATTGCTGCCTGCGTTGAAGGTGTCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTAt  <  2:682137/141‑1 (MQ=11)

TTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGG‑TTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTAT  >  CP000730/35947‑36226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`