BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	35,940	G→A	27.8%	R204R (CGC→CGT)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	35,940	0	G	A	27.8%	572.2 / 289.1	435	R204R (CGC→CGT)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base G (128/183); new base A (63/58); total (192/243)
Fisher's exact test for biased strand distribution p-value = 5.19e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.88e-01

Only 100 of 447 total aligned reads displayed.

GTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAA‑ATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGT  >  CP000730/35798‑36080

gtattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                                                                                                                                               >  2:529666/7‑141 (MQ=11)

 gattttaataaAAA‑CGATGGGTCTTTTGCAAAGT‑AAAAAACATAGCTAATCACGAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                                                                                                                                              <  1:347449/136‑1 (MQ=11)

  gATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                                                                                                                                               >  1:634764/1‑141 (MQ=255)

  attttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                                                                                                                                             <  1:417923/137‑1 (MQ=11)

   ttttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                                                                                                                                            >  2:326033/4‑141 (MQ=11)

   ttttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                                                                                                                                            <  1:71200/138‑1 (MQ=11)

   ttttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCGAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                                                                                                                                            >  1:748206/4‑141 (MQ=11)

     ttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGtt                                                                                                                                          <  2:41180/140‑1 (MQ=11)

       aataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCt                                                                                                                                        <  2:541797/141‑1 (MQ=11)

                  aaGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCCGAAGAGACCTGCGGTTCTTTTtatata                                                                                                                               <  1:219886/141‑1 (MQ=11)

                                                                     cAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTCA‑ATACATTCAATACCTTTTAAAGTATGCTTTGCCGTATTTATAc                                                                           >  2:175143/1‑141 (MQ=11)

                                                                     cAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAA‑ATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATAc                                                                           <  2:310403/141‑1 (MQ=11)

                                                                     cAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAA‑ATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATAc                                                                           <  2:698898/141‑1 (MQ=11)

                                                                       gtgtTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAA‑ATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACtt                                                                         <  2:302263/141‑1 (MQ=32)

                                                                        tgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGAATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAA‑ATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGGTACttt                                                                        >  1:761552/1‑141 (MQ=255)

                                                                         gtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGGATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAA‑ATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTg                                                                       <  1:315174/141‑1 (MQ=11)

                                                                         gtTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGCCCTGCGGTTCGTTTTATATAGAGCGTAA‑ATACACTCAGTACCTGTTAACGTATTCTTGTCTGTATTGATACGTTg                                                                       >  1:190730/1‑141 (MQ=11)

                                                                          ttCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAA‑ATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGa                                                                      <  1:128037/141‑1 (MQ=11)

                                                                          ttCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAA‑ATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa                                                                      <  2:687565/141‑1 (MQ=32)

                                                                          gtCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAA‑ATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGa                                                                      <  1:116320/140‑1 (MQ=11)

                                                                           tcgtttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAA‑ATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGAt                                                                     >  1:136512/5‑141 (MQ=12)

                                                                            cgttttaCTTGCTAGCATGATGCTAATTTCGTGGCATGGTGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAA‑ATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATa                                                                    <  2:620934/135‑1 (MQ=11)

                                                                            cGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAA‑ATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATa                                                                    >  2:229078/1‑141 (MQ=17)

                                                                             gtttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAA‑ATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATat                                                                   <  1:583868/139‑2 (MQ=255)

                                                                              tttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAA‑ATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAca                                                                  <  2:665725/140‑2 (MQ=17)

                                                                              tctAACTTGCTAGCATGAGTCTAAGTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAA‑ATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATatc                                                                  <  1:78181/139‑3 (MQ=11)

                                                                              cTTAACGTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAA‑ATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAcc                                                                  <  1:324343/141‑1 (MQ=17)

                                                                               ttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAA‑ATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCt                                                                 <  2:81161/141‑1 (MQ=17)

                                                                               ttAACTTGCTAGCAGGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAA‑ATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCt                                                                 <  2:719493/141‑1 (MQ=17)

                                                                                tAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAA‑ATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCtt                                                                <  2:606278/141‑1 (MQ=32)

                                                                                 aatttGCTAGCATGATGCTAATTTCGTGGCATTGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAA‑AGACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTg                                                               <  2:147731/138‑1 (MQ=255)

                                                                                   cTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAA‑ATACATTCAATACCTTTTAAAGTATTCTTTGCCGGATTGATACTTTGATATCTTGtc                                                             >  1:256046/1‑141 (MQ=11)

                                                                                   cTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATACGTAGATCTGAAGAGACCTGCGGGTCTGTTTATATAGAGCGTAA‑ATACACTCAATACCTTTTAAAGATATCTTTGCTGTATTGTTACTTTGATACCTTGtc                                                             >  2:384145/1‑141 (MQ=11)

                                                                                    ttGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAA‑ATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtct                                                            <  2:510762/141‑1 (MQ=17)

                                                                                      gCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAA‑ATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtcttt                                                          >  1:456200/1‑141 (MQ=17)

                                                                                       cTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAA‑ATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttc                                                         <  2:13942/141‑1 (MQ=17)

                                                                                       cTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTACTTTTTATATAGAGCGTAA‑ATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGGTACTTTGATACCTTGtctttc                                                         >  1:244059/1‑141 (MQ=12)

                                                                                         aGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAA‑ATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttctt                                                       <  1:199490/141‑1 (MQ=17)

                                                                                          gCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAA‑CTACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGttttttttt                                                      >  2:266376/1‑140 (MQ=11)

                                                                                          gCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAA‑ATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTa                                                      >  1:548663/1‑141 (MQ=32)

                                                                                          gCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAA‑ATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTa                                                      >  1:296025/1‑141 (MQ=17)

                                                                                           cATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAA‑ATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTAc                                                     >  1:670484/1‑141 (MQ=255)

                                                                                           cATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAA‑ATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTAc                                                     >  1:433368/1‑141 (MQ=32)

                                                                                             tgatgCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGCCCTACGGTTCTTTTTATATAGAGCGTAA‑ATACATCCACTACCTTTTAAAGTATTCTTTCCCGTATTGATACTTGCATTTCTTGTCTTTCTTCCtt                                                   >  2:116820/1‑141 (MQ=11)

                                                                                             tgatgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAA‑ATACACTCAATACATTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACtt                                                   >  2:554190/1‑141 (MQ=21)

                                                                                              gatgCTAATTTCGTGACATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAA‑ATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACttt                                                  >  1:336206/1‑141 (MQ=21)

                                                                                               gtgCTAATTTCGTGGCATGGGGAAAACCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAA‑ATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTa                                                 <  1:17371/140‑1 (MQ=11)

                                                                                               atgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAA‑ATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTAGTCTTTCTTACTTTa                                                 >  2:210125/1‑141 (MQ=21)

                                                                                                   tAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAA‑ATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAACTTGTCTTTCTTACTTTAatat                                             >  2:650796/1‑141 (MQ=21)

                                                                                                    aaTTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAA‑ATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGAAACTTTGAGACCTTGTCTTTCTTACATTAatatt                                            >  1:84821/1‑140 (MQ=255)

                                                                                                      tttCGTGGCATGGCGAAGATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAA‑ATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGAc                                          <  2:497054/141‑1 (MQ=255)

                                                                                                       ttCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAA‑ATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACg                                         >  2:483391/1‑141 (MQ=25)

                                                                                                         tgTGGCATGTGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAA‑ATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGt                                       <  2:344561/140‑1 (MQ=11)

                                                                                                          gTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAA‑ATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTg                                      >  2:249205/1‑141 (MQ=14)

                                                                                                          gTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAA‑ATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGACACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTg                                      <  1:598631/141‑1 (MQ=255)

                                                                                                          gTGGCATGGCGAAAATCCGTAGATCCGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAA‑ATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTg                                      >  1:100074/1‑141 (MQ=255)

                                                                                                           tGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAA‑ATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGa                                     <  1:207295/141‑1 (MQ=255)

                                                                                                              cATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAA‑ATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCt                                  >  2:219096/1‑141 (MQ=38)

                                                                                                              cATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAA‑ATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGATGATCt                                  <  2:108390/141‑1 (MQ=255)

                                                                                                               aTGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAA‑ATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCtt                                 <  2:442051/141‑1 (MQ=14)

                                                                                                               aTGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAA‑ATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCtt                                 <  1:614335/141‑1 (MQ=14)

                                                                                                               aTGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAA‑ATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCtt                                 >  1:74125/1‑141 (MQ=255)

                                                                                                                 ggggAAAATCCGTAGATCTGAACAGACCTACGGTTCTTTTT‑TTCACAGCGAAAGATACATTCAATACCTTTTAAATTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGc                               <  1:553948/138‑1 (MQ=14)

                                                                                                                 ggCGAAAATCCGTAGATCTGAAGAGGCCTGCGGTTCTTTTTATATAGAGCGTAA‑ATACAGTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTCGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGc                               <  2:280304/141‑1 (MQ=255)

                                                                                                                 ggCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAA‑ATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGc                               <  2:676293/141‑1 (MQ=255)

                                                                                                                 ggCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAA‑ATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGGGATCTTGc                               >  1:658843/1‑141 (MQ=255)

                                                                                                                   cGAAAATCCGTAGATCTGAATAGACCTGCGGTTCCTTTTATATAGAGCGTAA‑ATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTc                             <  1:463104/141‑1 (MQ=255)

                                                                                                                   cGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAA‑ATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGGTCTTGCTc                             >  1:235813/1‑141 (MQ=255)

                                                                                                                   cGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAA‑ATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTc                             >  1:379440/1‑141 (MQ=38)

                                                                                                                    gAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAA‑ATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCa                            <  1:304909/141‑1 (MQ=255)

                                                                                                                    gAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAA‑ATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCa                            >  2:360370/1‑141 (MQ=255)

                                                                                                                      aaaTCCGTAGATCTGAAGATACCTGCGGTTCTTTTTATATAGAGCGTAA‑ATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTGTAATATGACGGTGATCTTGCTCAAt                          <  2:7381/141‑1 (MQ=255)

                                                                                                                       aaTCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAA‑ATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATg                         >  1:147829/1‑141 (MQ=255)

                                                                                                                       aaTCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAAAGAGCGTAA‑ATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATGCTTTGATATCTTGTCTTTATTACTTTAATATGACGGTGATCTTGATCAATg                         >  1:167046/1‑141 (MQ=11)

                                                                                                                          ccGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAA‑ATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAgg                      <  1:226169/141‑1 (MQ=39)

                                                                                                                          ccGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAA‑ATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAgg                      <  2:730579/141‑1 (MQ=255)

                                                                                                                          ccGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGCAA‑CCACAGTCAATATCGTTTTAAGTATTCTTTGCTGTATTGATACTTCGATACCTTGTCTTTCTTACTCTAATATGACGGTGATCTTGCTCAATGAgg                      <  2:573131/141‑1 (MQ=14)

                                                                                                                           cGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAA‑ATGCCCAGCAAAGCTTTTAAAGTATTCTTTTCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGt                     <  1:717127/141‑1 (MQ=14)

                                                                                                                           cGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAA‑ATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTTCTTTAATATGACGGTGATCTTGCTCAATGAGGt                     >  2:429137/1‑141 (MQ=255)

                                                                                                                            gTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAA‑ATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACATTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGtt                    <  1:509858/141‑1 (MQ=255)

                                                                                                                             tAGACCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAA‑ATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATGCCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTa                   >  2:465696/1‑141 (MQ=255)

                                                                                                                               gATCTTAAGAGACCTACGGTTCTTTTTATATAGAGCGTAA‑ATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTAtt                 <  1:252375/141‑1 (MQ=255)

                                                                                                                                aTCTGAAGTGAGCTACGGTTCTTTTTATAGAAGGCGTAA‑ATACTTTCAATACTGTTTAAGGTATTCTTTGCCGTATGGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTc                <  2:18908/141‑1 (MQ=11)

                                                                                                                                aTCTGAAGAGACCTGCGGTTCTTTTTATCTAGAGCGTAA‑ATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGACACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTc                >  1:495149/1‑141 (MQ=255)

                                                                                                                                aTCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAA‑ATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTc                >  1:574678/1‑141 (MQ=255)

                                                                                                                                  cTGAAGAGCCCTGCGGTTCTTTTTATATAGAGCGTAA‑ATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTGAATATGACGGTGATCTTGCTCAATGAGGTTATTCAg              <  1:531082/141‑1 (MQ=255)

                                                                                                                                  cTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAA‑ATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAg              <  1:69382/141‑1 (MQ=255)

                                                                                                                                   tGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAA‑ATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGa             <  1:406047/141‑1 (MQ=37)

                                                                                                                                     aaGAGACCTGCGGTTCTTTTTATATAGAGCGTAA‑ATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGata           >  1:356354/1‑141 (MQ=38)

                                                                                                                                      agagACCTGCGGTTCTTTTTATATAGAGCGTAA‑ATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGatat          <  2:458020/141‑1 (MQ=255)

                                                                                                                                       gagaCCTGCGGTTCTTTTTATATAGAGCGTAA‑ATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATAtt         <  1:647034/141‑1 (MQ=255)

                                                                                                                                        agaCCTGCGGTTCTTTTTATATAGAGCGTAA‑ATACATTCAATACCTTTTAAAGTATTATTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATAttt        >  2:655729/1‑141 (MQ=38)

                                                                                                                                        agaCCTGCGGTTCTTTTTATATAGAGCGTAA‑ATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATAttt        >  2:712797/1‑141 (MQ=255)

                                                                                                                                          aCCTGCGGTTCTTTTTATATAGAGCGTAA‑ATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCg      <  1:16257/141‑1 (MQ=38)

                                                                                                                                           ccTGCGGTTCTTTTTATATAGAGCGTAA‑ATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGa     <  1:281050/141‑1 (MQ=255)

                                                                                                                                           ccTGCGGTTCTTTTTATATAGAGCGTAA‑ATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGa     <  1:474199/141‑1 (MQ=255)

                                                                                                                                           ccTGCGGTTCTTTTTATATAGAGCGTAA‑ATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGa     <  2:626174/141‑1 (MQ=255)

                                                                                                                                             tGCGGTTCTTTTTATATAGAGCGTAA‑ATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATg   <  2:757968/141‑1 (MQ=255)

                                                                                                                                             tGCGGTGCTTTTTATATAGAGCGTAA‑ATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATg   <  1:392136/141‑1 (MQ=255)

                                                                                                                                              gCGGTTCTTTTTATATAGAGCGTAA‑ATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGt  >  2:586482/1‑141 (MQ=255)

GTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAA‑ATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGT  >  CP000730/35798‑36080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`