BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,929,917	C→T	52.9%	intergenic (‑1000/‑168)	USA300HOU_1794 ← / → USA300HOU_1796	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,929,917	0	C	T	52.9%	13.2 / 213.7	159	intergenic (‑1000/‑168)	USA300HOU_1794/USA300HOU_1796	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (37/38); new base T (48/36); total (85/74)
Fisher's exact test for biased strand distribution p-value = 3.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01

Only 100 of 162 total aligned reads displayed.

GTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTAATTA  >  CP000730/1929777‑1930057

 tGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCa                                                                                                                                                    >  2:756786/1‑141 (MQ=255)

  gACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCAt                                                                                                                                                   <  1:418512/141‑1 (MQ=255)

  gACTGCGTTTGTATACTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTTGTCGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCAt                                                                                                                                                   <  1:1077/141‑1 (MQ=255)

     tgtgCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGC‑TTATTTGCGTTCCCATCATAGCACCAGTCATCTGTGGCTGTGCCAtttc                                                                                                                                                >  2:331535/1‑139 (MQ=255)

     tgtgCTTGTATATTTGCCGATGATATAATAGTAAAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGc                                                                                                                                                >  1:350511/1‑141 (MQ=255)

      atgCTTGTATATTCACAGATGGTATCATCATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGt                                                                                                                                              >  1:304451/2‑141 (MQ=255)

      atgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGt                                                                                                                                              <  2:661469/140‑1 (MQ=255)

      atgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGt                                                                                                                                              >  2:317567/2‑141 (MQ=255)

       tgCTTGTATATTTGGCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGt                                                                                                                                              <  2:286128/141‑1 (MQ=255)

       tgCTTGGATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGta                                                                                                                                             <  1:212214/141‑2 (MQ=255)

        gCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGTCATTGCGtt                                                                                                                                             <  2:690961/141‑1 (MQ=255)

         cTTGTATATTCACAGATGGTATAATAATAGAGGCGCATATCTGTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAA‑TCGTTTTAGTTAACATCATAGCACCAGTCATCAGAGACTGTGCTGTTGCGtatt                                                                                                                                           >  1:183114/1‑141 (MQ=255)

          ttGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGtttt                                                                                                                                           <  2:236979/141‑1 (MQ=255)

           tGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattat                                                                                                                                         >  1:762306/1‑139 (MQ=255)

           tGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattat                                                                                                                                         <  2:685505/141‑6 (MQ=255)

            gtatattcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑TTTGCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattata                                                                                                                                        >  2:255734/12‑138 (MQ=255)

             tataTTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑TGCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatac                                                                                                                                       <  1:384396/141‑8 (MQ=255)

              atattcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatact                                                                                                                                      >  2:452646/10‑136 (MQ=255)

               tatgcggcGATGATATAATAGTAGAGCCGCCTATCTCTCAGGTGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCt                                                                                                                                      <  1:94473/134‑1 (MQ=255)

                 ttcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAt                                                                                                                                   >  2:482053/7‑141 (MQ=255)

                 ttcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAt                                                                                                                                   <  2:641712/135‑1 (MQ=255)

                 ttcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑TGCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATCCTTAt                                                                                                                                   >  2:116941/7‑141 (MQ=255)

                  tcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAtt                                                                                                                                  <  1:588051/136‑1 (MQ=255)

                  tcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAA‑TCATTTTCGTTCACATCATAGCACCGGTCATCAGTGGCGGTGCTGTTGCGTATTATACTTAtt                                                                                                                                  >  1:23374/6‑141 (MQ=255)

                   cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTg                                                                                                                                 >  1:744835/5‑141 (MQ=255)

                   cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAA‑TCATTTTCGTTCACATCATAGCACTAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTg                                                                                                                                 >  1:26139/5‑141 (MQ=255)

                   cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAA‑TCATTTTCGTTCACATCATAGCACTAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTg                                                                                                                                 >  1:46857/5‑141 (MQ=255)

                   cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTg                                                                                                                                 >  2:254977/5‑141 (MQ=255)

                     cagatggtATAATAATATAGTGGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCAGCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc                                                                                                                               <  2:183178/139‑1 (MQ=255)

                     cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc                                                                                                                               >  1:692460/3‑141 (MQ=255)

                     cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc                                                                                                                               >  1:493152/3‑141 (MQ=255)

                      agatggtATAATAATCGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGTAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAA‑TCATTTTCGTTCACATCATAGCACGAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGACt                                                                                                                              >  2:214743/2‑141 (MQ=255)

                           gtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGTGAGTTGGGGCA‑TTA‑C‑TTTGCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttatta                                                                                                                         >  1:720412/2‑136 (MQ=255)

                           gtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttcgta                                                                                                                         >  2:210700/2‑136 (MQ=255)

                            tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTggtctagtaa                                                                                                                        >  2:15738/1‑133 (MQ=255)

                            tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaa                                                                                                                        <  1:642279/141‑2 (MQ=255)

                              taataaTAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAAc                                                                                                                      >  2:300211/1‑141 (MQ=255)

                               aataatAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACg                                                                                                                     >  2:173057/1‑141 (MQ=255)

                                ataatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                                                                                                    <  1:608995/137‑1 (MQ=255)

                                ataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                                                                                                    <  2:740733/141‑1 (MQ=255)

                                ataCTAGAGTCGCCCACTTCTCACGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑TGCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                                                                                                    <  2:597991/141‑1 (MQ=255)

                                 taatagAGTCGCCTATCTCTCGGGCGGCAATT‑‑GACGAAGCGAGAAGGAATAGTTC‑T‑T‑TGCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa                                                                                                                   <  1:362513/138‑1 (MQ=255)

                                  aatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑a                                                                                                                <  1:323271/139‑1 (MQ=255)

                                  aatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑a                                                                                                                <  1:702817/139‑1 (MQ=255)

                                   gtagAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCa‑‑a                                                                                                                <  1:103884/141‑1 (MQ=255)

                                   atagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑ac                                                                                                               <  2:371298/140‑2 (MQ=255)

                                   atagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑TGCTAA‑TAATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑ac                                                                                                               >  2:195353/2‑140 (MQ=255)

                                     agagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAAAGCa‑‑acaa                                                                                                             >  2:283390/1‑138 (MQ=255)

                                     agagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaa                                                                                                             >  1:13729/1‑138 (MQ=255)

                                      gagTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATAc                                                                                                             <  2:708963/141‑1 (MQ=255)

                                      gagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaa                                                                                                            <  2:521906/141‑5 (MQ=255)

                                      gagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaa                                                                                                            >  1:719709/1‑137 (MQ=255)

                                       agTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACa                                                                                                            >  1:132538/1‑141 (MQ=255)

                                        gTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAC‑G‑C‑T‑CTGCGTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAACTAGACGCA‑‑ATACaa                                                                                                           <  1:402258/141‑1 (MQ=255)

                                        gTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACaa                                                                                                           <  1:193340/141‑1 (MQ=255)

                                         tCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataa                                                                                                         >  1:653965/1‑134 (MQ=255)

                                         gcGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACaa‑‑a                                                                                                        <  1:79128/140‑1 (MQ=255)

                                          cGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataat                                                                                                        >  1:645293/1‑133 (MQ=255)

                                           gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataatt                                                                                                       >  2:412835/1‑132 (MQ=255)

                                           gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataatt                                                                                                       <  1:317320/141‑10 (MQ=255)

                                           gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataatt                                                                                                       <  2:725208/141‑10 (MQ=255)

                                           gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTGTACTTATTGGCTTAGTAAACGCa‑‑acaaataatt                                                                                                       >  2:499373/1‑132 (MQ=255)

                                           gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataatt                                                                                                       <  2:409843/141‑10 (MQ=255)

                                            ccTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataatta                                                                                                      >  2:266377/1‑131 (MQ=255)

                                             cTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataattaa                                                                                                     <  1:627713/141‑12 (MQ=255)

                                              tATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAgg                                                                                                   >  1:454/1‑141 (MQ=255)

                                              tATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTCGCGTATTATACTTATTGGCTTAGCAAACGCa‑‑acaaataattaat                                                                                                    <  1:467103/141‑13 (MQ=255)

                                              tATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑TGCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaagtaattaat                                                                                                    >  1:286993/1‑129 (MQ=255)

                                               ttctctcAGGCGTTAATTTTGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGC‑TTTTTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTCCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGt                                                                                                  <  2:51561/140‑1 (MQ=255)

                                               aTCTCCCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAA‑TCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa‑‑acaaataattaata                                                                                                   <  1:416478/141‑14 (MQ=255)

                                                    tcAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGAC‑at                                                                                            <  2:36661/141‑1 (MQ=255)

                                                     cAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGAC‑ata                                                                                           >  2:495019/1‑141 (MQ=255)

                                                     cAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAC‑TCATTTTAGTTCACATCCTAGCACCAGTCATCAGTGGCTGTGCTGTTCCGTATTATACTTATTGGCTTAGTAAACGCA‑‑ACACAT‑‑AAttaataggcgac                                                                                           >  2:111110/1‑130 (MQ=255)

                                                            cAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑Gc                                                                                  <  1:668149/141‑1 (MQ=255)

                                                            cAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATACGTGAC‑ATAT‑AGCC‑Gc                                                                                  >  1:517510/1‑141 (MQ=255)

                                                                ttAGACGCAGAGGGGAGGTGTA‑TAA‑G‑G‑TGATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCa                                                                              <  1:146770/141‑1 (MQ=255)

                                                                ttAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCa                                                                              <  1:123006/141‑1 (MQ=255)

                                                                ttAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCa                                                                              <  1:742979/141‑1 (MQ=255)

                                                                 tAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑Gccacaa                                                                             >  1:3115/1‑136 (MQ=255)

                                                                  aGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGAC‑GTAT‑AGCC‑GCACCAAt                                                                            <  1:568180/141‑1 (MQ=255)

                                                                    aCGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATaa                                                                          <  1:27185/141‑1 (MQ=255)

                                                                        agagagGAGGTGTA‑TAA‑G‑G‑TGATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAAt                                                                      >  1:503593/1‑141 (MQ=255)

                                                                         gagagGAGGTGTA‑TAA‑G‑G‑TGATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AACAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATc                                                                     <  2:582040/141‑1 (MQ=255)

                                                                                       taA‑G‑G‑TGATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGc                                                        <  1:576517/141‑1 (MQ=255)

                                                                                        aa‑G‑G‑TGATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCa                                                       <  2:676258/141‑1 (MQ=255)

                                                                                        aa‑G‑G‑GGATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCa                                                       <  1:233930/141‑1 (MQ=255)

                                                                                                       tATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAggg                                            <  1:756786/141‑1 (MQ=255)

                                                                                                        aTTTTCGTTCACATGATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGGGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAgggg                                           <  1:247488/141‑1 (MQ=255)

                                                                                                        aTTTTCGTCCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAgggg                                           <  1:165511/141‑1 (MQ=255)

                                                                                                        aTTTTCGATCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTATTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAgggg                                           >  1:484195/1‑141 (MQ=255)

                                                                                                         ttttCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGa                                          >  1:281437/1‑141 (MQ=255)

                                                                                                              gTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGgt                                     >  1:90260/1‑141 (MQ=255)

                                                                                                                     tcatAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTATATAAg                              >  1:693435/1‑141 (MQ=255)

                                                                                                                        tAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTaa                           >  1:686362/1‑141 (MQ=255)

                                                                                                                         aGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTaaa                          >  2:692661/1‑141 (MQ=255)

                                                                                                                          gCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCAACAAATAATTAATAGGCGACTTTATAAGCCAGCATCGA‑AAAAATCCCCACACTACTGC‑AATAGTGTGGGGATTGGTGcattaa                              >  2:23121/1‑138 (MQ=255)

                                                                                                                                  catcaGTGGCTGGGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTAttt                 <  1:727494/141‑1 (MQ=255)

                                                                                                                                     caGTGGCTGTGTCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATAATTATTTTCg              <  2:260684/141‑1 (MQ=255)

                                                                                                                                         ggCTGTGCCATTGCGTTTTTTTCTTATTGGCGAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGt          <  2:578995/141‑1 (MQ=255)

                                                                                                                                               gCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTAAt    <  2:653597/141‑1 (MQ=255)

GTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGC‑TTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCA‑‑ATACAA‑‑AATAGGTGAC‑ATAT‑AGCC‑GCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTAATTA  >  CP000730/1929777‑1930057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`