BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,929,870	A→C	59.1%	intergenic (‑953/‑215)	USA300HOU_1794 ← / → USA300HOU_1796	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,929,870	0	A	C	59.1%	116.7 / 117.7	131	intergenic (‑953/‑215)	USA300HOU_1794/USA300HOU_1796	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (26/27); new base C (46/30); total (74/57)
Fisher's exact test for biased strand distribution p-value = 2.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 131 total aligned reads displayed.

AATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAG  >  CP000730/1929739‑1930009

aaTCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGCTTAttt                                                                                                                                    <  2:55256/141‑1 (MQ=255)

 aTCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGGGGAGGTGTA‑TAA‑G‑G‑G‑GGTGCTTGtttt                                                                                                                                   >  1:67697/1‑141 (MQ=255)

              aaTCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGCTAAAAGATTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATa                                                                                                                      <  1:625249/141‑1 (MQ=255)

              aaTCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATa                                                                                                                      <  1:40282/141‑1 (MQ=255)

              aaTCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATa                                                                                                                      <  1:40471/141‑1 (MQ=255)

               aTCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAg                                                                                                                     >  1:104477/1‑141 (MQ=255)

               aTCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAg                                                                                                                     >  2:651616/1‑141 (MQ=255)

                    ataACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCAATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCa                                                                                                                >  1:456894/1‑141 (MQ=255)

                             cAGAAGCAAGTGACTGTGCTTGTTCTTGAACCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGt                                                                                                       >  1:661663/1‑141 (MQ=255)

                             cAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGt                                                                                                       >  2:655242/1‑141 (MQ=255)

                              aGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGCTTATTTTGGTTCACATCATAGCACCAGGAATCAGTg                                                                                                      >  1:255828/1‑141 (MQ=255)

                               atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                                                                                                    >  1:621917/15‑141 (MQ=255)

                                  tgaagtcgatatccTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgt                                                                                                 <  1:509801/128‑1 (MQ=255)

                                  ggaagtaggtatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgt                                                                                                 <  1:82708/130‑1 (MQ=255)

                                  ggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgt                                                                                                 >  1:168329/12‑141 (MQ=255)

                                  gCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACGTAATAGCAGCAGTCATCAGTGGCtg                                                                                                  >  2:189474/1‑141 (MQ=255)

                                  gCAAGTGACTGTGCTTGTATATTTACCGATGATATAATAGTAGAGTCGCCTATCTCGCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtg                                                                                                  >  2:490596/1‑141 (MQ=255)

                                    aagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                                                                                               <  1:442291/132‑1 (MQ=255)

                                    aaGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                                                                                                >  1:291877/1‑141 (MQ=255)

                                     agtagatattcTTCTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGct                                                                                              <  2:500018/131‑2 (MQ=255)

                                     agtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGct                                                                                              <  2:618990/133‑2 (MQ=255)

                                     agtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAATCATTGTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGct                                                                                              <  1:430107/133‑2 (MQ=255)

                                      gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                                                                                             <  1:543834/134‑3 (MQ=255)

                                      gcagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                                                                                             >  2:578301/8‑139 (MQ=255)

                                        gACTGTGCTTGTATATTTGCCGATCATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCCCATCATAGCACCAGTCAGCAGTGGCTGTGCCAt                                                                                            <  1:134979/141‑1 (MQ=255)

                                        agatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgct                                                                                           >  1:476726/6‑137 (MQ=255)

                                          atatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttgc                                                                                         <  1:603429/138‑7 (MQ=255)

                                           tatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCGGTGGCTGTGCTGTTGCg                                                                                        >  1:117086/3‑141 (MQ=255)

                                            ttgCTTGTATAGTTGCCGATGATATAATAGTAGCGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCg                                                                                        <  2:101255/140‑1 (MQ=255)

                                            gtgCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCg                                                                                        >  1:416956/1‑141 (MQ=255)

                                            atgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGt                                                                                       <  2:516675/140‑1 (MQ=255)

                                             tgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGta                                                                                      >  1:32426/1‑140 (MQ=255)

                                               cTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatt                                                                                    <  1:557506/141‑4 (MQ=255)

                                                ttGTATATTCACAGATGGGATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCCGTCATCAGTGGCTGTGCTGTTGCGtatta                                                                                   <  1:594485/141‑2 (MQ=255)

                                                  gTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GCTAATCATTTTCGTTCACATAATAGCACCAGTCATCAGTGGCCGTGCTGTTGCGtattata                                                                                 >  2:374439/1‑138 (MQ=255)

                                                   tatattcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtgttatac                                                                                >  1:187644/11‑137 (MQ=255)

                                                   tatattcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatat                                                                                >  2:605751/11‑137 (MQ=255)

                                                   tatattcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑T‑TGGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatac                                                                                >  1:451585/11‑137 (MQ=255)

                                                   tatattcacagatggtATAATAATAGAGTCGCCTATCTCTCAGACGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAATAATTTTCGTTCACATCATAGCACCAGGCATCAGTGGCTGTGCTGTTGCGtattatac                                                                                >  2:605744/11‑137 (MQ=255)

                                                   tataTTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATTCTTATCTTCGTTCGCATCATAGCACCAGTCATCAGTGCCTGTGCCATTGCGttttttt                                                                                 <  1:407313/141‑1 (MQ=255)

                                                    atattcacagatggtATAATAATAGAGTCGCGTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGAGCATTAC‑T‑T‑T‑GCTAATCATTTTCGTACACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatact                                                                               >  1:491760/10‑136 (MQ=255)

                                                     aatTTGCCGATGATATAATAGGAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCt                                                                               <  2:262162/140‑1 (MQ=255)

                                                      tatcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTa                                                                             >  2:337992/8‑141 (MQ=255)

                                                      attcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTa                                                                             >  2:121783/8‑141 (MQ=255)

                                                      atTTGCCGATGATATAATAGTAGAGTCGACTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCtt                                                                              <  1:463886/141‑1 (MQ=255)

                                                          acagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGCGGAGGTGCATTAC‑T‑T‑T‑GCTAGTCCTTGTCGCTCACATCAGAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTgg                                                                         >  2:640275/4‑141 (MQ=255)

                                                          acagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTgg                                                                         <  1:252100/138‑1 (MQ=255)

                                                           cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc                                                                        <  2:591541/139‑1 (MQ=255)

                                                           cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc                                                                        >  1:9688/3‑141 (MQ=255)

                                                            cGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGc                                                                        <  2:40546/141‑1 (MQ=255)

                                                               tggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAg                                                                    >  1:183012/4‑141 (MQ=255)

                                                                ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATTATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                                   <  2:20863/139‑1 (MQ=255)

                                                                ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                                   >  2:621136/3‑141 (MQ=255)

                                                                ggtacaatgttagAGTCGCCTATCTCCTAGGCGGCACTT‑‑GCCGAAGAGAGGAGGTGCATTCC‑T‑T‑T‑GCTAGTCATTTCCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                                   <  2:112381/131‑1 (MQ=255)

                                                                 gtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAATCATTTTCGCTCACCTCAAAACACCTGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTa                                                                  >  1:126114/2‑141 (MQ=255)

                                                                   ctaataaGAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaa                                                                <  1:533308/140‑3 (MQ=255)

                                                                   ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaa                                                                >  1:328466/1‑139 (MQ=255)

                                                                    taataaTAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaac                                                               <  1:220128/141‑4 (MQ=255)

                                                                    taataaTAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAAGCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATAATTATTGGCttggtaaac                                                               >  1:91844/1‑133 (MQ=255)

                                                                     aataatACAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACg                                                              <  2:430551/141‑1 (MQ=255)

                                                                      ataatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                                             >  2:336579/5‑141 (MQ=255)

                                                                      ataGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTacctcgacc                                                              >  2:429641/1‑133 (MQ=255)

                                                                       aaatagAGGCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa                                                            <  2:581824/138‑1 (MQ=255)

                                                                          tagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaaca                                                         >  1:293777/1‑139 (MQ=255)

                                                                          tagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaaca                                                         <  2:299261/141‑3 (MQ=255)

                                                                           agagTCGCCTCTCTCTCCGGCGTCAATT‑‑GACGACGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCAGTGCTGTTGCGTCTTATACTTATTGGCTTAGTAAACGccacaa                                                        >  1:190893/1‑136 (MQ=255)

                                                                            gagTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATAc                                                        <  1:459516/141‑1 (MQ=255)

                                                                            gagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaa                                                       >  2:282973/1‑137 (MQ=255)

                                                                             agTCCCCTATCTCTCAGGCGTCAATTTATACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACa                                                       <  1:65601/141‑1 (MQ=255)

                                                                              gTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAACCGCaacaaata                                                     >  1:213076/1‑135 (MQ=255)

                                                                              gTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaata                                                     >  2:430302/1‑135 (MQ=255)

                                                                                 gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                                  <  1:464365/141‑10 (MQ=255)

                                                                                 gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                                  >  2:154913/1‑132 (MQ=255)

                                                                                 gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑TTT‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGGTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                                  <  1:456075/141‑10 (MQ=255)

                                                                                   cTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCGGTCGTCATCGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaa                                                >  1:154206/1‑130 (MQ=255)

                                                                                   cTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGGGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaa                                                <  2:45839/141‑12 (MQ=255)

                                                                                    tATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaaa                                               >  2:269331/1‑129 (MQ=255)

                                                                                    tATCTCTCAGGCGTAAATT‑‑GACGAAGAGAGGAGGTGCA‑TAACT‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCCCCAGTCATCAGGGGCTGTGCTGTTGCGTATTATACTTATTGGCTGAGTAAACGCaacaaataattaat                                               <  1:603319/141‑13 (MQ=255)

                                                                                     aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTACTCATTTTCTTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                                              <  1:349945/141‑14 (MQ=255)

                                                                                     aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                                              >  1:377099/1‑128 (MQ=255)

                                                                                      tctctcAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCCCATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGCCTAAGTAGCAGCCATACAACATAGGTg                                              >  2:528254/1‑141 (MQ=255)

                                                                                      tctctcAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattcatag                                             >  2:195512/1‑127 (MQ=255)

                                                                                         ctcAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACa                                           >  2:389607/1‑141 (MQ=255)

                                                                                         cccacgCGTCAATTTATCCGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCAGCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACa                                           <  1:401522/136‑1 (MQ=255)

                                                                                           cAGGCGTCAATTTAGACGAAGAGAGGAGGTGTA‑GAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACata                                         <  2:33137/141‑1 (MQ=255)

                                                                                               cggcAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGc                                     <  2:463808/138‑1 (MQ=255)

                                                                                                     tttAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCAcc                               <  1:425465/141‑1 (MQ=255)

                                                                                                     tttAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTCCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTATGTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCAcc                               >  2:368015/1‑141 (MQ=255)

                                                                                                           cGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCGAGGTAGATGCAATACAAAATAGGTGCCGTTTAGCCGCACCATTaga                         >  1:17030/1‑139 (MQ=255)

                                                                                                            gCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATaaaa                        >  2:659479/1‑141 (MQ=255)

                                                                                                            gCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTAACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTTTTGGCTAAGTAGACGGAATACATAATAGGTGACATATAGCCGCACCAATaaaa                        >  2:321984/1‑141 (MQ=255)

                                                                                                                   ggaggTGTA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCt                 <  2:543100/141‑1 (MQ=255)

                                                                                                                       gtgtA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACt             >  1:231736/1‑141 (MQ=255)

                                                                                                                          ta‑taA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTAcc          >  1:620603/1‑141 (MQ=255)

                                                                                                                          ta‑taA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTAcc          >  1:281535/1‑141 (MQ=255)

                                                                                                                             taA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGc        <  1:494761/141‑1 (MQ=255)

                                                                                                                                 g‑g‑T‑GAGTCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCaaa     <  2:232352/141‑1 (MQ=255)

                                                                                                                                       gATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAACATAGGAGTCATAGAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAg  <  1:293784/141‑1 (MQ=255)

                                                                                                                                       gATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGACATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAg  <  1:437436/141‑1 (MQ=255)

                                                                                                                                       gATGCTCATTTTCGTTCACATCAGAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAg  <  1:2760/141‑1 (MQ=255)

AATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAG  >  CP000730/1929739‑1930009

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`