Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,000,857	1	.	C	30.8%	19.9 / 15.4	13	noncoding (535/2921 nt)	rrl3	23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base . (7/2);  new base C (4/0);  total (11/2)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AGCACACGGTTTCAGGTTCTATTTCACTCCCC‑T‑TCCGGGGTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACT‑AGAGAGTATTTAGCCTTAGGA‑GATGGTCCTCCCAGATTCCGACGGAATTTCACGTGTTCCGTCGTACTCAGGATCCACTCAAGAGAG  >  CP000730/2000825‑2000996                                   |                                                                                                                                              cgtacacGGTTTCAGGTTCTATTTCACTCCCC‑TCACAGGGGTTCTTTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGTCAG‑TAGTATTTAGCCTT‑GGAGGATGGTCCCCCCATATTCAGACAGGATATCACGTGTCCcgc                           >  1:120805/4‑147 (MQ=2)   cacaCGGTTTCAGGTTCTATTTCACTCCCC‑T‑TCCGGGGTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACT‑AGAGAGTATTTAGCCTTAGGA‑GATGGTCCTCCCAGATTCCGACGGAATTTCACGTGTTCcgtcgt                        >  1:317030/1‑148 (MQ=32)     cacGGTTTCAGGTTCTATTTCACTCCCC‑T‑TCCGGGGTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACT‑AGAGAGTATTTAGCCTTAGGA‑GATGGTCCTCCCAGATTCCGACGGAATTTCACGTGTTCCGTCGTAc                      >  1:205965/1‑148 (MQ=32)     cacGGTTTCAGGTTCTATTTCACTCCCC‑T‑TCCGGGGGGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACT‑AGAGAGTATTTAGCCTTAGGA‑GATGGTCCTCCCAGATTCCGACGGAATTTCACGTGTTCCGTCGTAc                      >  2:120263/1‑148 (MQ=17)        ggTTTCAGGTTCTATTTCACTCCCC‑TCACAGGGGTTCTTTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGT‑CAGTAGTATTTAGCCTTGGAG‑GATGGTCCCCCCATATTCAGACAGGATATCACTTGTCCCGCCCTACTc                    >  1:317467/1‑148 (MQ=2)        ggTTTCAGGTTCTATTTCACTCCCC‑TCACAGGGGTTCTTTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGT‑CAGTAGTATTTAGCCTTGGAG‑GATGGTCCCCCCATATTCAGACAGGATATCACGTGTCCCGCCCTACTc                    >  1:205522/1‑148 (MQ=2)           ttCAGGTTCTATTTCACTCCCC‑T‑TCCGGGGTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACT‑AGAGAGTATTTAGCCTTAGGA‑GATGGTCCTCCCAGATTCCGACGGAATTTCACGTGTTCCGTCGTACTCAGGa                <  2:292042/148‑1 (MQ=32)                gTTCTATTTCACTCCCC‑T‑TCCGGGGTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACT‑AGAGAGTATTTAGCCTTAGGA‑GATGGTCCTCCCAGATTCCGACGGAATTTCACGTGTTCCGTCGTACTCAGGATCCAc           <  1:345683/148‑1 (MQ=32)                    tATTTCACTCCCCAC‑ACAGGGGTTCTTTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGT‑CAGTAGTATTTAGCCTTGGAG‑GATGGTCCCCCCATATTCAGACAGGCTATCACGTGTCCCGCCCTACTcgatttcactgaa        >  2:116113/1‑136 (MQ=2)                    tATTTCACTCCCC‑TCACAGGGGTTCTTTTCGCCTTTCCCTCACGGTACTGGTTCACTATCGGTCAGT‑CAGTAGTATTTAGCCTTGGAG‑GATGGTCCCCCCATATTCAGACAGGATATCACGTGTCCCGCCCTACTcgatttcactgaa        >  2:224754/1‑136 (MQ=2)                       ttCACTCCCC‑T‑TCCGGGGTGCTTTTCACCTTTCCTTCACGGTACTGGTTCACTATCGGTCACT‑AGAGAGTATTTAGCCTTAGGA‑GATGGTCCTCCCAGATTCCGACGGAATTTCACGTGTTCCGTCGTACTCAGGATCCACTCAagag    >  2:273728/1‑148 (MQ=17)                         cACTCCCC‑T‑TCCGGGGTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACT‑AGAGAGTATTTAGCCTTAGGA‑GATGGTCCTCCCAGATTCCGACGGAATTTCACGTGTTCCGTCGTACTCAGGATCCACTCAagagag  >  2:344064/1‑148 (MQ=32)                         cACTCCCC‑T‑TCCGGGGTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACT‑AGAGAGTATTTAGCCTTAGGA‑GATGGTCCTCCCAGATTCCGACGGAATTTCACGTGTTCCGTCGTACTCAGGATCCACTCAagagag  >  2:96601/1‑148 (MQ=32)                                   |                                                                                                                                              AGCACACGGTTTCAGGTTCTATTTCACTCCCC‑T‑TCCGGGGTGCTTTTCACCTTTCCCTCACGGTACTGGTTCACTATCGGTCACT‑AGAGAGTATTTAGCCTTAGGA‑GATGGTCCTCCCAGATTCCGACGGAATTTCACGTGTTCCGTCGTACTCAGGATCCACTCAAGAGAG  >  CP000730/2000825‑2000996

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑