| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 2,709,596 | Δ1 bp | 100% | coding (1331/1812 nt) | oatA ← | possible acetyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 2,709,596 | 0 | T | . | 94.7% | 77.7 / ‑0.8 | 19 | coding (1331/1812 nt) | oatA | possible acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base . (8/10); total (8/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.64e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch. | |||||||||||
TTGCGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCATGTTGTTTGCC > CP000730/2709460‑2709720 | ttGCGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGACtttta > 2:68311/1‑140 (MQ=255) cacaATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCctct > 2:130250/1‑141 (MQ=255) acaATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCtt < 1:126584/141‑1 (MQ=255) aaTTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTg < 2:87016/141‑1 (MQ=255) ccAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCt < 1:90555/141‑1 (MQ=255) cTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATc > 2:10478/1‑141 (MQ=255) tttACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCt > 1:107419/1‑141 (MQ=255) tACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCttt < 2:10123/141‑1 (MQ=255) aCCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCtttt > 2:16206/1‑141 (MQ=255) aTCAATTTGTGCATTTGGTATTTTCTTCGTAAAGACATTACCAATCTCCACCATGGCCGAGACACCACTCAATAGTGGTGATGAC‑TTTTGATATCCTCTTTGCTATTCGCTGTCTGCTTGTCCTCTTGCTTATCTGTTTTa > 2:93992/1‑141 (MQ=255) cATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACtttttt > 1:16371/1‑141 (MQ=255) tttCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCgttgtt < 1:130250/141‑1 (MQ=255) ttAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTaa > 2:121356/1‑141 (MQ=255) aGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCg < 2:82949/141‑1 (MQ=255) gACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGtc > 1:11812/1‑141 (MQ=255) accaTGACAGCGTCACCACTTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTATTTTAACGACTTTTTTCTTCGTTGTTTTACATTCCGTCTTCTTCTCTTtcttct < 2:117407/141‑1 (MQ=255) cGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTATCGACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCa < 2:88906/141‑1 (MQ=255) cGAGTCACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCa < 2:33244/141‑1 (MQ=255) cACCAATTAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCAtgttg < 2:83950/141‑1 (MQ=255) ttAATAGTGGTGATGAC‑TTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCATGTTGTTTGcc < 1:10478/141‑1 (MQ=255) | TTGCGATTTCACAATTGGTGTAGCATCAACGAGTTGCCGTCCAACTTTACCATCAATTTGTGCATTTGGTATTTTCTTAGTAAAGACATTACCAATATCCACCATGACCGAGTCACCAATTAATAGTGGTGATGACTTTTTAATATCCTCTTTGCTATTCGCTGTCTGCTTATCCTCTTGCTTATCTTTTTTAACGACTTTTTTCTTCGTTGTTTTAAATTCCGTCTTCTTCTCTTTCTTCTCGGCTTCATGTTGTTTGCC > CP000730/2709460‑2709720 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |