New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP000730	= 225539	37 (0.970)	4 (0.110)	4/262	5.6	9.7%	coding (626/897 nt)	murQ	possible sugar phosphate isomerase
	?	CP000730	= 225543	39 (1.080)					coding (630/897 nt)	murQ	possible sugar phosphate isomerase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

ATCTATTTCATGCAATGAACATGCAGTTATAAGTGAAATTGCGCAGTATCCAGTAGAAGTTAAAGTTGGTCCAGAAGTATTAACTGGTTCAACACGTTTAAAGTCTGGTACAGCACAAAAGTTAATTTTAAATATGATTTCAACCAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  CP000730/225393‑225539 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑caaccatTGTGATGGTTGAAATCATATTTAAAATTAACTTTTGTGCTGTACCAGACTTTAA  <  CP000730/225543‑225490                                                                                                                                                                                                           ATCTATTTCATGCAATGAACATGCAGTTATAAGTGAAATTGCGCAGTATCCAGTAGAAGTTAAAGTTGGTCCAGAAGTATTAACTGGTTCAACACGTTTAAAGTCTGGTACAGCACAAAAGTTAATTTTAAATATGATTTC                                                              >  1:408243/1‑141                  AACATGCAGTTATAAGTGAAATTGCGCAGTATCCAGTAGAAGTTAAAGTTGGTCCAGAAGTATTAACTGGTTCAACACGTTTAAAGTCTGGTACAGCACAAAAGTTAATTTTAAATATGATTTCAACCATTGTGATGGTTG                                             <  1:226712/141‑1                        aaaTTATAAGTGAAATTGCGCAGTAACCAGTAGAAGTTAAAGTTGGTCCAGAAGTAGTAACTGGTTCAACACGTTTAAAGTATAAAAAAAAACAAAAGTTAATTTTAAATATGATTTCAACCATTGTGATGGTTGAAATCA                                       <  2:372654/138‑1                                                                    GGTCCAGAAGTATTAACTGGTTCAACACGTTTAAAGTCTGGTACAGCACAAAAGTTAATTTTAAATATGATTTCAACCATTGTGATGGTTGAAATCATATTTAAAATTAACTTTTGTGCTGTACCAGACTTTAA  <  1:397815/134‑1                                                                    GGTCCAGAAGTATTAACTGGTTCAACACGTTTAAAGTCTGGTACAGCACAAAAGTTAATTTTAAATATGATTTCAACCATTGTGATGGTTGAAATCATATTTAAAATTAACTTTTGTGCTGTACCAGACTTTAA  >  2:397815/1‑134                                                                                                                                                                                                           ATCTATTTCATGCAATGAACATGCAGTTATAAGTGAAATTGCGCAGTATCCAGTAGAAGTTAAAGTTGGTCCAGAAGTATTAACTGGTTCAACACGTTTAAAGTCTGGTACAGCACAAAAGTTAATTTTAAATATGATTTCAACCAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  CP000730/225393‑225539 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑caaccatTGTGATGGTTGAAATCATATTTAAAATTAACTTTTGTGCTGTACCAGACTTTAA  <  CP000730/225543‑225490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.