BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,269	C→T	56.8%	A95T (GCA→ACA)	tpn ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,269	0	C	T	56.8%	64.7 / inf	375	A95T (GCA→ACA)	tpn	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base C (74/86); new base T (91/119); total (166/209)
Fisher's exact test for biased strand distribution p-value = 5.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01

Only 100 of 378 total aligned reads displayed.

CTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATG‑TATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGG  >  CP000730/36131‑36408

caacctttctggAAGGTGCCTGAAATGTAATTACCTTTGGAGGTTTAGCAACTTGTTTAATGCGACGTTTGATAAACGAATATATTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATG‑TATGTCCCTCTGCa                                                                                                                                            <  1:75118/129‑1 (MQ=11)

caaCCTTCGGTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATG‑TATGTCCCTCTGCa                                                                                                                                            <  1:155918/138‑1 (MQ=18)

 tACCTTCTGTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATG‑TATGTCCCTCTGCAt                                                                                                                                           <  2:212362/140‑1 (MQ=18)

  gtcTTCGTTGAATGTGCCTGATCTGTAACTACCTGTTGGGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATG‑TATGTCCCTCTGTATc                                                                                                                                          <  1:50968/138‑1 (MQ=11)

  gccTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATG‑TATGTACCTCTGTATc                                                                                                                                          >  2:13422/2‑140 (MQ=11)

   ccTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATG‑TATGTCCCTCTGTATCa                                                                                                                                         >  1:142336/1‑140 (MQ=17)

   ccTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACACAATATCTAATG‑TATGTCCCTCTGTATCa                                                                                                                                         >  2:199721/1‑140 (MQ=12)

   acTTCCTTGAAGGTGCCTGATCTGTAATTACCTTTGGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATACGCTGACTCCTTATCTCGTTGCTTACGCAACCAAATATCTAATG‑TATGTCCCTCTGCATCa                                                                                                                                         <  2:159726/139‑1 (MQ=11)

      tctttGAAGGTGCCTGATGTGTAACTACCTTTTGAGTCTTACCAAATTGTTTAATGCGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATG‑TATGTCCCTCTGTATCAATg                                                                                                                                      <  1:106326/137‑1 (MQ=11)

      tCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATG‑TCTGTCCCTCTGCCTCTATg                                                                                                                                      >  1:27888/1‑140 (MQ=12)

             aGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATATAATG‑TATGTCCCTCTGTATCAATGGCACGat                                                                                                                               >  2:117853/1‑140 (MQ=11)

                  ccTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATG‑TATGTCCCTCTGCATCAATGGCACGATATaaa                                                                                                                          >  1:253768/1‑140 (MQ=32)

                   cTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTACTG‑TCTGTCCCTCTGTATCAATGGCACGATATAAAt                                                                                                                         <  2:275125/140‑1 (MQ=11)

                       tCTGTAATTAACTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAAAACATATGCTGACTGATTATCTCGTAGCTTACGCAACCAAATATCTAATG‑TCAGTCCGCCTGCGTAATAGGCACGATATAAACAgat                                                                                                                     >  2:282700/1‑138 (MQ=11)

                        cggTAAATACCTTTTGAGGCTTACCAAATTGTTTACTGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATG‑TATGTCCCTCTGTATCAATGGCACGATATAAATAGCTc                                                                                                                    <  1:257187/138‑1 (MQ=11)

                        cTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCCACCAAATGTCTACTG‑TCTGTCCCTCTGCCTCAGTGGCACGATGTAGATAGCTc                                                                                                                    >  1:23320/1‑140 (MQ=11)

                        cTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATG‑TATGTCCCTCTGCATCAATGGCACGATATAAATAGCTc                                                                                                                    >  1:288488/1‑140 (MQ=32)

                        cTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATG‑TATGTCCCTCTGTATCAATGGCACGATATAAATAGCTc                                                                                                                    >  2:115223/1‑140 (MQ=255)

                         tGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATG‑TATGTCCCTCTGCATCAATGGCACGATATAAATAGCTcc                                                                                                                   <  1:15178/140‑1 (MQ=33)

                         tGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATG‑TATGTCCCTCTGTGTCAATGGCACGATATAAATAGGTcc                                                                                                                   >  2:59876/1‑140 (MQ=11)

                          gtactcaCCTTTTGAGGTTTACCAAAGTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATG‑TATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCa                                                                                                                  <  2:204534/134‑1 (MQ=16)

                             aTTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATG‑TATGTCCCTCTGTATCACTGGCGCGATATAAATAGCTCCAttt                                                                                                               >  1:92941/1‑140 (MQ=12)

                              ctACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGCATGATTATCTCGTTGCTTAAGCAACCAAATATCTAATG‑TATGTCCCTCTGTATCAATGGTACGATCTTAATAGCTcccattt                                                                                                              >  2:11931/2‑135 (MQ=11)

                              ctACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTACTGTTTTGTCCACCT‑TATCAATGGCACGATATAAATAGCTCCAtttt                                                                                                              >  1:298320/2‑140 (MQ=11)

                               tACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATG‑TATGTCCCTCTCGATCAACGACACGATAGAAAAAACGCCAtgtcc                                                                                                             >  1:305957/1‑136 (MQ=11)

                               tACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATG‑TATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTc                                                                                                             <  2:222163/140‑1 (MQ=16)

                                aCCTTTTTAGGTTCACCAAATTGTTTAATGAGACGTTTGATAAACTCATACGCTGAATGATTACCTCGTTGCTTACGCAACCAAATATCTAATG‑TATGTCCCTCTAAAACAATTTCACCATTTAAATAGCGCCATTTTcc                                                                                                            <  2:133289/140‑1 (MQ=11)

                                aCCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATG‑TATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTcc                                                                                                            <  2:163542/140‑1 (MQ=18)

                                aCCTTTTGAGGGTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATG‑TATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTcc                                                                                                            <  2:125235/140‑1 (MQ=12)

                                   ttttGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATG‑TATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCttt                                                                                                         >  2:129899/1‑140 (MQ=18)

                                    tttGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATG‑TATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCtttt                                                                                                        >  1:220550/1‑140 (MQ=12)

                                    tttGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATG‑TATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCtttt                                                                                                        <  2:13529/140‑1 (MQ=12)

                                     ttGAGGTTTACCAAATTGTTTCCTGCGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATG‑TATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCAATTTTCCttttc                                                                                                       >  2:147423/1‑139 (MQ=255)

                                      tGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATG‑TATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTAt                                                                                                      >  2:315048/1‑140 (MQ=18)

                                       ggggTTTACCAAATTTTTTAATGAGACGTGTGATAAACGCATCTGCTGAATGATTATCTCGGTGCTTACGCAACCAAATATCTAATG‑TATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTAtt                                                                                                     <  1:42267/138‑1 (MQ=11)

                                       gAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACTCAACCAAATATCTAATG‑TATGTCCCTCTGTATCTATTGCACGATATAATTAGTGCCATTTTCCTTTTAtt                                                                                                     >  1:264750/1‑140 (MQ=11)

                                       gAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATG‑TATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATCTACCTTTTGtt                                                                                                     <  2:196664/140‑1 (MQ=255)

                                          gcttACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATG‑TATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTg                                                                                                  >  1:301610/3‑140 (MQ=18)

                                          gcttACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATG‑TATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTg                                                                                                  >  1:70223/3‑140 (MQ=17)

                                           tttAACAAATTGGTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATG‑TATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGa                                                                                                 <  1:178399/140‑1 (MQ=18)

                                            ttACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATG‑TATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGAt                                                                                                <  1:262359/140‑1 (MQ=18)

                                              aCCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATG‑TATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTAtt                                                                                                     >  2:252547/1‑133 (MQ=18)

                                              aCCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATG‑TATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGAtat                                                                                              <  2:100470/140‑3 (MQ=17)

                                               ccAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAATTATCTAATG‑TATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATTTa                                                                                             >  1:100747/1‑140 (MQ=17)

                                                 aaaTTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATG‑TATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACg                                                                                           >  1:286025/1‑140 (MQ=32)

                                                   tttggtcaaTGCGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATG‑TATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGtc                                                                                         <  1:115626/133‑1 (MQ=255)

                                                   aTTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATG‑TATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGtc                                                                                         >  1:274277/1‑140 (MQ=18)

                                                   aTTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATG‑TATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGtc                                                                                         >  2:39508/1‑140 (MQ=12)

                                                   aTTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATG‑TATGTCCCCCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGtc                                                                                         >  2:37106/1‑140 (MQ=255)

                                                    ttttgtAATGAGACGTTTGATAAACGCCTTTGCTTAATGATTATCTCGTTGCTTACGCAACCAAATAGCTAATG‑TATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGtct                                                                                        <  1:148362/135‑1 (MQ=11)

                                                       tttAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATG‑TATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCtcat                                                                                     <  2:298481/140‑1 (MQ=33)

                                                         tAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATG‑TATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCtcttca                                                                                   >  1:284098/1‑140 (MQ=255)

                                                         tAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATG‑TATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCtcatca                                                                                   <  1:124302/140‑1 (MQ=17)

                                                           aggcgagctttGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATG‑TATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAAt                                                                                 <  2:149169/132‑1 (MQ=11)

                                                           aTGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATG‑TATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAAt                                                                                 <  1:240284/140‑1 (MQ=33)

                                                            tGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATG‑TATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATa                                                                                <  2:94188/140‑1 (MQ=12)

                                                                  gTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATG‑TATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCCTCAATACGCCAt                                                                          >  2:261418/1‑140 (MQ=17)

                                                                     tGATAAACGCATATGCTGAATGATTAGCTCGTTGCTTACGCAACCAAATATCTAATG‑TATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTg                                                                       >  2:191528/1‑140 (MQ=17)

                                                                          aaCGCATATTCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATG‑TATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGtaata                                                                  <  2:54164/140‑1 (MQ=255)

                                                                          aaCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATG‑TATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGtaata                                                                  <  1:79525/140‑1 (MQ=33)

                                                                           aCGCATATTTTGAATCATTATCTCGTTGCTTACGCAACTAAATTTCTAATG‑TCTCTCCCTCTGTATCCATGGCACGATATATGTAGCTCCATTTTCCTTTTATTTTGCTATACTTCTCATCAATACGCCATTTGtaataa                                                                 <  2:278752/140‑1 (MQ=11)

                                                                           aCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATG‑TATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGtaataa                                                                 >  1:224839/1‑140 (MQ=34)

                                                                            cGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATG‑TATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAg                                                                >  2:140348/1‑140 (MQ=19)

                                                                             gCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATG‑TATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGc                                                               <  2:237261/140‑1 (MQ=255)

                                                                               atatGATGAATGATTATCTCGTTACTTACGCAACCAAATATCTAATG‑TATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATAAACGTCTCATCAATAAGCTATTTGTCATAAACtt                                                             >  2:271117/1‑140 (MQ=11)

                                                                                  tGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATG‑TATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCttatg                                                          >  2:184937/1‑137 (MQ=25)

                                                                                  agCTGAACGATTATCTCGTTGCTTACGCAACCAAATATCTAATG‑TATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCttttt                                                          <  1:162512/139‑1 (MQ=37)

                                                                                    cTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATG‑TATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTa                                                        >  2:240477/1‑140 (MQ=255)

                                                                                      gAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATG‑TATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATg                                                      <  2:302088/140‑1 (MQ=255)

                                                                                        aTGATTATCTCGTTGCTTACGCAACCAAATATCTAATG‑TATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCt                                                                                                           <  2:155617/85‑1 (MQ=25)

                                                                                             tATCTCGTTGCTTACGCAACCAAATATCTAATG‑TATGTCCCTCTGCATCAATGGCACGATATAAATTGCTCCATTTTCTTTTTATTTTGATGTACGTCTCATCATTATGTCATTTTTAATAAGGTTTTTTATGCTTTttc                                               >  1:19830/1‑140 (MQ=14)

                                                                                               tctcGTTGCTTACGCAACCAAATATCTAATG‑TATGTCCCTCTGCATCAATGGCACGATATAAATATCTCCTTTTTCCTTTTATTTTGATTTATGTCTTATCTATACGCCATCTGTAATCAGCTTTTTTATGCTGtttttt                                             >  1:8523/1‑140 (MQ=11)

                                                                                                 tcGTTGCTTACGCCACCCACTATCTAATG‑GATGTCTCCCTGGATCCATGGCGCGTTATCCATAGCTCCATTTTCCTTTTCTTTGGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTcc                                           <  2:64888/140‑1 (MQ=14)

                                                                                                 ccattGCTTACGGAACTAAATATCTAATG‑TATGTCCCTCTTCATTACTGGCTCTATATAAATATCTCCTTTTTCCTTTTTTTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTcc                                           <  2:81440/137‑1 (MQ=14)

                                                                                                  cGTTGCTTACGCAACCAAATATCTAATG‑TATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCAATTTTTATTAAGCTTTTTTATGCTTTTTTTTCCa                                          >  1:53703/1‑140 (MQ=255)

                                                                                                    tttcTTACGCAACCAAATATTTAATG‑TATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCaaa                                        <  2:211349/137‑1 (MQ=255)

                                                                                                       cTTACGCAACCAAATATCTAATG‑TATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAAttt                                     >  1:255342/1‑140 (MQ=40)

                                                                                                           cGCAACGAAATATCTAATG‑TATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATa                                 >  2:201747/1‑140 (MQ=255)

                                                                                                            gccaCCAAATATCTAATG‑TATTTCCCTCTGTATAAATGGCACGATATAAATAGCTCCATTTTCCGTTTATTTTGATTTACGTCGCATCAATACGCCATTTGTAATAAGCTTTTTTAGGCTTTTTCTTCCAAATTTGATat                                <  2:73580/137‑2 (MQ=11)

                                                                                                            gCAACCAAACATCTAATG‑TATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATat                                <  1:11931/140‑2 (MQ=255)

                                                                                                             cAACCAATTATCTAATG‑TCTGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATata                               <  1:182470/140‑3 (MQ=11)

                                                                                                             cAACCAAATATCTAATG‑TATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATata                               <  1:173314/140‑3 (MQ=255)

                                                                                                             cAACCAAATATCTAATG‑TATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACATCTCATCAATACGCCGTTTGTAATAAGCTTTTTTATGGTTTTTCTTCCAAATTCGATatc                               >  1:131228/1‑138 (MQ=14)

                                                                                                               aCCAAATATCCAATG‑TATGTCCCTCTGCATCCATGGCCAGCTATCCATCGGTGCTTATCGCATTTAGGTTGAAGTAAGTATCATCATTTCGCCAGTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATaaa                             <  2:306941/140‑1 (MQ=2)

                                                                                                                 cAAATATCTAATG‑TATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGAAATAAAAt                           >  1:96061/1‑140 (MQ=255)

                                                                                                                 cAAATATCTAATG‑TATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCCTTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTTCAAATTTGATATAAAAt                           >  2:8938/1‑140 (MQ=255)

                                                                                                                   aaTATCTAATG‑TATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCCAATTTGATATAAAATTg                         >  2:143023/1‑140 (MQ=255)

                                                                                                                   aaTATCTAATG‑TATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTTATCAATACGCCTTTTGTTATAAGCTTTTTTATCCTTTTTCTTCTTATTTTGATatcagtttg                         >  1:112812/1‑132 (MQ=14)

                                                                                                                    atatCTAATG‑TATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCTAAATTTGATACAAAATTgg                        <  1:191528/140‑1 (MQ=255)

                                                                                                                     tatCTAATG‑TATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTggg                       <  1:60444/140‑1 (MQ=255)

                                                                                                                     tatCTAATG‑TATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTGCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAACAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTggg                       <  2:176516/140‑1 (MQ=255)

                                                                                                                         tAATG‑TTTGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCat                   <  1:282700/140‑1 (MQ=255)

                                                                                                                         tAATC‑TATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCat                   <  2:232825/140‑1 (MQ=255)

                                                                                                                                 tgtCCTTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTg            <  1:247539/140‑1 (MQ=255)

                                                                                                                                 tgtCACTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTg            <  1:23064/140‑1 (MQ=255)

                                                                                                                                  gtCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGa           <  1:316122/140‑1 (MQ=21)

                                                                                                                                   tCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATACAATTGGGGCATATTCTTGaa          >  2:286318/1‑140 (MQ=255)

                                                                                                                                   tCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGaa          >  2:49739/1‑140 (MQ=255)

                                                                                                                                   tCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGaa          >  2:22903/1‑140 (MQ=255)

                                                                                                                                      ctctGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAAccc       <  1:202569/140‑1 (MQ=255)

CTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATG‑TATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGG  >  CP000730/36131‑36408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`