| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 638,614 | G→T | 100% | T14K (ACA→AAA) | thiD ← | phosphomethylpyrimidine kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 638,614 | 0 | G | T | 100.0% | 74.7 / ‑6.5 | 26 | T14K (ACA→AAA) | thiD | phosphomethylpyrimidine kinase |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base T (15/10); total (16/10) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CATCCATTGGTAATGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAAT‑TCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTGTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAATATTTGATGA > CP000730/638477‑638740 | cATCCATTGGTAATGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAAT‑TCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTttgt > 1:110419/1‑141 (MQ=255) ctCCATTGGTAATGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAAT‑TCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTc < 1:288887/140‑1 (MQ=255) cgcccgtGGCAATGGTGTAACATCGTGTTACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAAT‑TCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTc < 1:22541/135‑1 (MQ=255) ccccACTGGTAATGGTGTAACATCGTGCTACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAAT‑TCTTGCAACGTTCTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTc < 1:22478/139‑1 (MQ=255) cATTGGTAATGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCTACCATGCCATAGGTATCGAAT‑TCTTGGAACGTTTTCAAATCTGCTTGCATCCCTGCGCCAGCACTTGTGTCAGa > 1:169783/1‑141 (MQ=255) ttGGTAATGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAAT‑TCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCTGAAc > 2:157681/1‑141 (MQ=255) tAATGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATAGCACACGTTACTAAT‑TCTTGGAACGGTTTCAAATCTGCTTGCATACCAGCGCCAGCACTTTTTTAAGAACCGGc > 2:230662/1‑141 (MQ=255) cATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAAT‑TCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTaaaa > 1:345449/1‑141 (MQ=255) cATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAAT‑TCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGACCGAACCGGCAATTGTTaaaa > 1:169494/1‑141 (MQ=255) aTCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAAT‑TCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCTGCAATTGTTAAAAc < 1:199433/141‑1 (MQ=255) gACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAAT‑TCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAActttcttt < 1:118846/141‑1 (MQ=255) aCCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAAT‑TCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTa > 1:182970/1‑141 (MQ=255) tATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAAT‑TCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGc > 2:161448/1‑141 (MQ=255) tCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAAT‑TCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTCAAACTTTCTTTAAAGCCATTCAGCtt > 2:4702/1‑141 (MQ=255) aGTAACGGGGGCAGTTAAAGCGACCATGCCATACGTATCTAAT‑TCTTTGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTc < 2:102895/141‑1 (MQ=255) aGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAAT‑TCTTGGACAGCTTGCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTc < 1:296695/141‑1 (MQ=255) gTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAAT‑TCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTcc > 1:155269/1‑141 (MQ=255) aCGATGGCAGTTAAAGCGACCATGCCATACGTATCTAAT‑TCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCCGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTGCACTCCTAc > 2:262060/1‑141 (MQ=255) cGATGGCAGTTAAAGCGACCATGCCATACGTATCTAAT‑TCTTGGAACGTTTTCAAATCTTCTTGCATACCTGCGCCAGCACTTTTGTCAGAATCGGGAATTGTTAAAACTTTCTTTAAAGACATTGAGATTCACTCGTACa > 2:156547/1‑141 (MQ=255) gATGGCAGTTAAAGCGACCATGCCATACGTATCTAAT‑TCTTGGAACGTTTTCAAATCTGCTTGCTTACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCActactaaaa > 2:68234/1‑138 (MQ=255) cAGTTAAAGCGACCATGCCATAAGCAT‑TAATGTCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACataataa > 1:91649/1‑141 (MQ=255) tGCCATACGTATCTAAT‑TCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTcatcat > 1:372266/1‑141 (MQ=255) ctatccGTATCTAAT‑TCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCatcatat < 1:122609/136‑1 (MQ=255) ttGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTg < 2:110419/141‑1 (MQ=255) gTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTCCATAATAATATTGTATTCATCATTTCATTTTTAACTTAATGGaaaactt > 1:97171/1‑138 (MQ=255) cAAATCTGCTTGCATACCTGCGCCAGCACTTTTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAg > 1:105447/1‑141 (MQ=255) cTGCGCCAGCATGTTTGCTAGACCCGGCTATTGTTATAACTTTCTTTAAAGCCATTGAGTTTCACTACTACATAATAATATTGTATTCATCATATCATTTTTAGGCTGATTGAAAAATATTACGTATTCGATATTtgatga < 2:112797/141‑1 (MQ=255) | CATCCATTGGTAATGGTGTAACATCGTGTGACCATGTATCTTTATCCATAGTAACGATGGCAGTTAAAGCGACCATGCCATACGTATCTAAT‑TCTTGGAACGTTTTCAAATCTGCTTGCATACCTGCGCCAGCACTTGTGTCAGAACCGGCAATTGTTAAAACTTTCTTTAAAGCCATTGAGCTTCACTCCTACATAATAATATTGTATTCATCATATCATTTTTAACCTAATTGAAAAATATTAAGCATTCAATATTTGATGA > CP000730/638477‑638740 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |