BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,929,925	0	T	A	65.5%	380.0 / inf	344	intergenic (‑1008/‑160)	USA300HOU_1794/USA300HOU_1796	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (59/58); new base A (135/87); total (199/145)
Fisher's exact test for biased strand distribution p-value = 8.31e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.84e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 361 total aligned reads displayed.

CTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGC‑AATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTAATTATACTGC  >  CP000730/1929786‑1930063

cTTGTATATTCACAGATGGTATAGTAATAGGGTCGCCGATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGGGGGGCATTAC‑T‑T‑TGCTAATCGTTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatt                                                                                                                                           <  1:101703/141‑1 (MQ=255)

 ttGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGtttt                                                                                                                                           <  1:988902/141‑1 (MQ=255)

  tGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGttttt                                                                                                                                          >  2:515219/1‑141 (MQ=255)

   gTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGtttttt                                                                                                                                         <  2:831638/141‑1 (MQ=255)

   gTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGtttttt                                                                                                                                         <  2:638786/141‑1 (MQ=255)

    tataTTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGttttttt                                                                                                                                        <  2:180408/141‑1 (MQ=255)

      tattcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACtt                                                                                                                                     >  1:1033474/9‑141 (MQ=255)

        ttcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAt                                                                                                                                   >  1:745376/7‑141 (MQ=255)

         ttGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTAt                                                                                                                                   <  2:808584/141‑1 (MQ=255)

         ttGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTAt                                                                                                                                   <  1:90676/141‑1 (MQ=255)

         tcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAtt                                                                                                                                  <  1:534030/136‑1 (MQ=255)

          cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTg                                                                                                                                 >  1:1170285/5‑141 (MQ=255)

          cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATGTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTg                                                                                                                                 >  2:1031840/5‑141 (MQ=255)

          cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTg                                                                                                                                 >  1:130881/5‑141 (MQ=255)

          cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑TTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTg                                                                                                                                 >  2:470278/5‑141 (MQ=255)

           ccagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTgg                                                                                                                                <  1:267072/138‑1 (MQ=255)

           acagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTgg                                                                                                                                <  1:1000429/138‑1 (MQ=255)

           acagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTgg                                                                                                                                <  1:361485/138‑1 (MQ=255)

            cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc                                                                                                                               <  1:919852/139‑1 (MQ=255)

            cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc                                                                                                                               <  1:307221/139‑1 (MQ=255)

             agatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt                                                                                                                              >  1:682909/2‑141 (MQ=255)

             agatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt                                                                                                                              <  2:903913/140‑1 (MQ=255)

             agatcgtataATACTATAGTCGCCGATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt                                                                                                                              <  2:481699/135‑1 (MQ=255)

              ggtggtataATAATAGAGGCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGGGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtt                                                                                                                             <  2:129821/136‑2 (MQ=255)

              gatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtt                                                                                                                             <  2:325815/141‑2 (MQ=255)

                tggtataATAATAGCGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGACGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTCTTGGCttag                                                                                                                           <  1:175583/138‑4 (MQ=255)

                tggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttag                                                                                                                           <  2:274086/138‑4 (MQ=255)

                tggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttag                                                                                                                           <  1:408428/138‑4 (MQ=255)

                tggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttag                                                                                                                           <  2:413987/138‑4 (MQ=255)

                tggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttag                                                                                                                           <  2:148531/138‑4 (MQ=255)

                 ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑TTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                                                                                          >  1:1165538/3‑141 (MQ=255)

                 ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑TTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                                                                                          <  1:691474/139‑1 (MQ=255)

                  gtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTa                                                                                                                         >  2:847665/2‑141 (MQ=255)

                   tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGTAGGGGCATTAC‑T‑T‑TGCTAATCATCTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaa                                                                                                                        <  1:29432/141‑2 (MQ=255)

                   tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaa                                                                                                                        <  2:1086553/141‑2 (MQ=255)

                    ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaa                                                                                                                       <  1:204299/141‑3 (MQ=255)

                    ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaa                                                                                                                       <  2:621543/141‑3 (MQ=255)

                    acaataatAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaa                                                                                                                       >  2:324546/3‑139 (MQ=255)

                       ataatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCGCATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                                                                                                    >  1:156047/5‑141 (MQ=255)

                       ataatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                                                                                                    >  1:494416/5‑141 (MQ=255)

                       ataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                                                                                                    <  1:444214/141‑1 (MQ=255)

                       ataGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCTTCGCACCAGTCATCAATGGCTGTGCCACTGCGGTTGTTGCTGATTGGCTacgaaaacg                                                                                                                     >  1:215403/1‑133 (MQ=255)

                        taatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGcc                                                                                                                   >  2:993547/4‑140 (MQ=255)

                        taatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGC‑a                                                                                                                  >  2:391123/4‑141 (MQ=255)

                        taatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGC‑a                                                                                                                  <  2:932052/138‑1 (MQ=255)

                        taatagAGTCCCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTACATTTATTGGCTTAGTAAACGC‑a                                                                                                                  <  2:258990/138‑1 (MQ=255)

                         aatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGC‑aa                                                                                                                 <  1:954760/139‑1 (MQ=255)

                         aatagAGTCGCCTATCTCTCAGGCGTCAA‑‑TCGACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGC‑aa                                                                                                                 <  2:886531/139‑1 (MQ=255)

                          gtagCGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGC‑aa                                                                                                                 <  1:719221/141‑1 (MQ=255)

                          atagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATGATACTTATTGGCTTAGTAAACGC‑aac                                                                                                                >  2:677617/2‑140 (MQ=255)

                           tagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGC‑aaca                                                                                                               <  2:448196/141‑3 (MQ=255)

                            agagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGC‑aacaa                                                                                                              >  2:541061/1‑138 (MQ=255)

                             gagTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGC‑AATAc                                                                                                              <  2:646807/141‑1 (MQ=255)

                               gTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTCGTAAACGC‑aacaaata                                                                                                           >  2:288442/1‑135 (MQ=255)

                                tCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGGGTA‑TAA‑G‑G‑TGGGGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGGTTTTTTCTTATTGGCTAAGTAGACGC‑AATACaaa                                                                                                           >  1:39451/1‑141 (MQ=255)

                                tCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGCGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCCGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGccacaaataa                                                                                                           >  2:494644/1‑132 (MQ=255)

                                tCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGC‑aacaaataa                                                                                                          >  2:1016792/1‑134 (MQ=255)

                                 cGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGC‑aacaaatcat                                                                                                         <  2:1168645/141‑9 (MQ=255)

                                   ccTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGC‑AATACAAAATa                                                                                                        <  2:342872/141‑1 (MQ=255)

                                   ccTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGC‑agccaatactta                                                                                                       >  2:776929/1‑130 (MQ=255)

                                   ccTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGC‑aacaaataatta                                                                                                       >  1:251373/1‑131 (MQ=255)

                                   ccTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGC‑aacaaataatta                                                                                                       >  1:620119/1‑131 (MQ=255)

                                    cTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGC‑AATACAAAATAg                                                                                                       >  1:1110546/1‑141 (MQ=255)

                                    cTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGC‑aacaaataattaa                                                                                                      >  2:954167/1‑130 (MQ=255)

                                    cTATCGCTCAGGCGTCAATT‑‑GGCGAAGAGAGGAGGTGCATTAC‑C‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGC‑aacaaataattaa                                                                                                      <  1:426498/141‑12 (MQ=255)

                                     tATCTCTCAGGCGTGAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGC‑aacaaataattaat                                                                                                     >  1:664766/1‑129 (MQ=255)

                                     tATCTCTCAGGCGTCTATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATATTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATAGTTATTGGCTTAGTAAACGC‑aacaagtaattaat                                                                                                     >  2:976937/1‑129 (MQ=255)

                                     tATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGC‑AATACAAAATAgg                                                                                                      >  2:1200657/1‑141 (MQ=255)

                                     tATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGC‑aacaactaattaat                                                                                                     >  2:196464/1‑129 (MQ=255)

                                     tATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGC‑aacaaataattaat                                                                                                     <  1:968682/141‑13 (MQ=255)

                                     tATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGC‑aacaaataattaat                                                                                                     >  1:1098997/1‑129 (MQ=255)

                                      aTCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGC‑AATACAAAATAggg                                                                                                     >  2:655623/1‑140 (MQ=255)

                                      aTCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGC‑AATACAAAATAGGt                                                                                                     <  2:700839/141‑1 (MQ=255)

                                      aTCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCAAAAGTTTTAATGGTCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGC‑AATACAAAATAGGt                                                                                                     >  1:571682/1‑141 (MQ=255)

                                      aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGC‑aacaaataattaata                                                                                                    >  2:746350/1‑128 (MQ=255)

                                      aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGC‑aacaaataattacta                                                                                                    >  1:798969/1‑128 (MQ=255)

                                             aGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGC‑AATACAAAATAGGTGACatat                                                                                              >  1:1184292/1‑141 (MQ=255)

                                             aGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGC‑AATACAAAATAGGTGACatat                                                                                              <  1:831787/141‑1 (MQ=255)

                                                      ttgaaacccAGATAGTAGGTTTA‑TCA‑T‑G‑TGATGCTGATTTTCTTTCACATCATAGCACCAGTCACCAGTGGCTGGGCCATTGCCTTTTTTTCTTATTGGCTAAGTAGACGC‑AATACAAAATAGGTGACATATAGCCGCAcc                                                                                     <  2:166057/133‑1 (MQ=255)

                                                                  gaggagGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCCCCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGC‑AATACAAAATAGGTGACATATAGCCGCACCAATAAAAATccc                                                                         <  2:595820/141‑1 (MQ=255)

                                                                                    g‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGC‑AATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAAt                                                          >  1:809439/1‑141 (MQ=255)

                                                                                      ggATGGTTATTTTGGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGC‑AATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATa                                                         <  2:366859/140‑1 (MQ=255)

                                                                                             tATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGC‑AATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAggg                                                  <  1:78494/141‑1 (MQ=255)

                                                                                             tATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGC‑AATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAggg                                                  <  1:655623/141‑1 (MQ=255)

                                                                                               tgttCGTTGCCATCATAGCACCAGTCATCAGTGGCTCCGCCATTGCGTTTTTTTCTTATTGGCTTAGTAGACGC‑AATACCAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGa                                                <  2:1132925/139‑1 (MQ=255)

                                                                                                 ttCGTTCACGTCATAGCACCAGTCATCGGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGC‑AATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATGGTGAGGGGAtt                                              <  2:737542/141‑1 (MQ=255)

                                                                                                    gTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGC‑AATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCCCAAATAGTGAGGGGATTGgt                                           >  1:932609/1‑141 (MQ=255)

                                                                                                         catcatAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGC‑AATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGtata                                      >  1:1090867/1‑141 (MQ=255)

                                                                                                          atcatAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGC‑AATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATaa                                     >  2:920101/1‑141 (MQ=255)

                                                                                                                  aCCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGC‑AATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATGGTGGTATAAGTAAATAc                             >  1:1066775/1‑141 (MQ=255)

                                                                                                                  aCCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGC‑AATACAAAATAGGTGACATATAGCCGCACCAATAAAAAACCCCGCACGACCGCAAATAGTGAGGGGATTGGTGtaacagaaagtac                             >  1:516523/1‑130 (MQ=255)

                                                                                                                        catcaGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGC‑AATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTAttt                       <  1:1128586/141‑1 (MQ=255)

                                                                                                                         atcaGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGC‑AATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTAtttt                      >  1:1042621/1‑141 (MQ=255)

                                                                                                                         atcaGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGC‑AATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTAtttt                      >  1:459405/1‑141 (MQ=255)

                                                                                                                             gTGGATGTGCCATTGCTTTTATTTATAATTGGCTAAGTA‑CCGCAAATACAAAATAGGTGAAATATACCCGCACCAATAAAAATCCCCTCACTAGCGCGAATAGTGAGGGGATTGGTGTATAAGTAAATTCTTATTTTCGtt                  <  2:1098612/141‑1 (MQ=25)

                                                                                                                                gCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGC‑AATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTAGTGTATAAGCAAATACTTATTTTCGTTGTc               >  2:418690/1‑141 (MQ=255)

                                                                                                                                  tgtgCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGC‑AATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCACCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCtt             >  1:776026/1‑141 (MQ=255)

                                                                                                                                    tgCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGC‑AATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGCGAGGGGAGTGGTGTATAAGTAAAAACTTATTTTCGTTGTCTTaa           >  1:637908/1‑141 (MQ=255)

                                                                                                                                    tgCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGC‑AATACAAAATAGGTAAAATATAGTTGCATCACTAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGtagactgta           >  1:227364/1‑133 (MQ=255)

                                                                                                                                             gTTTTTTTCTTATTGGCTAAGTAGACGC‑AATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGGGGGGATTGGTGTATAAGTACATACTTATTTTCGTTGTCTTAATTATACTgt  >  2:556912/1‑140 (MQ=255)

CTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGC‑AATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAGTGAGGGGATTGGTGTATAAGTAAATACTTATTTTCGTTGTCTTAATTATACTGC  >  CP000730/1929786‑1930063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`