BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,929,797	G→A	33.9%	intergenic (‑880/‑288)	USA300HOU_1794 ← / → USA300HOU_1796	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,929,797	0	G	A	33.9%	128.2 / 77.2	109	intergenic (‑880/‑288)	USA300HOU_1794/USA300HOU_1796	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (37/34); new base A (14/23); total (52/57)
Fisher's exact test for biased strand distribution p-value = 2.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 109 total aligned reads displayed.

TTGTCATAATAAAATCGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTA  >  CP000730/1929658‑1929934

tagTCATAATAATATCGCCGACTTGCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTCTGCTTGTATATTTGc                                                                                                                                                >  2:452381/3‑141 (MQ=255)

  gTCATAATAAAATCGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCg                                                                                                                                              >  2:742496/1‑141 (MQ=255)

  gTCATAATAAAATCGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCg                                                                                                                                              <  1:33594/141‑1 (MQ=255)

    cATAATAAAATCGCCTACTTTCTTAAATTAATAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCgat                                                                                                                                            <  2:409284/141‑1 (MQ=255)

    cATAATAAAATCGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCgat                                                                                                                                            <  1:143628/141‑1 (MQ=255)

           aaaTCGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATataa                                                                                                                                     >  2:492273/1‑141 (MQ=255)

            aatatccTATTTTCTGTAAATAACATGATCTATTCTCAGACAACTTGTCCAATTAAGTGTAGACGATTCAATCTATCATCTCGAATCATATAACTTATCCGAAGCAAGTGACTGTGCTTGTATATTTGCCGATTATataat                                                                                                                                    <  2:519461/136‑1 (MQ=25)

              tCGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAAtag                                                                                                                                  <  1:346892/141‑1 (MQ=255)

                cccTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAAGTAAGTGTAGACGATTCCATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAAtagta                                                                                                                                <  1:763590/140‑1 (MQ=255)

                  cTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTTTATTTGCCGATGATATAATAGTaga                                                                                                                              >  1:268565/1‑141 (MQ=255)

                        tCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGcc                                                                                                                        >  1:638656/1‑141 (MQ=255)

                         cTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCt                                                                                                                       >  2:72687/1‑141 (MQ=255)

                         cTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCt                                                                                                                       <  2:764321/141‑1 (MQ=255)

                         cTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCt                                                                                                                       <  2:602467/141‑1 (MQ=255)

                         cTTAAATTAACAATATCTATTCTCATAGAATTTGTCAAATTAAGTGTAGACGATTCAATCTATCAGCTAGATTCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTTCCGATGATATAATAGTAGAGTCGACt                                                                                                                       >  2:333251/1‑141 (MQ=255)

                           tAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTAt                                                                                                                     >  2:534176/1‑141 (MQ=255)

                                 aaCAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCa                                                                                                               <  2:769535/141‑1 (MQ=255)

                                        tCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCa                                                                                                       <  1:695758/141‑1 (MQ=255)

                                           aTTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAAtt                                                                                                    >  1:379884/1‑141 (MQ=255)

                                              ctcATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAg                                                                                                 >  1:668581/1‑141 (MQ=255)

                                                cATAGAATTTGTCCAATTAAGTGTAGACGATTCAAACCATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGAc                                                                                               <  1:394381/141‑1 (MQ=255)

                                                   aGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATCTAATAGGAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCa                                                                                            >  2:687706/1‑141 (MQ=255)

                                                   aGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCGAGAATCATATAACTTATCAGAGGCAAGTGACTGTGCTTGTATATTTGCCGATGAGATAATATTGGAGTCGCCTATCTCTAAGGCG‑TCAATTGAGCCGCa                                                                                            >  2:396959/1‑141 (MQ=255)

                                                    gAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCag                                                                                           <  2:692173/141‑1 (MQ=255)

                                                    gAATTTGGCCAATTAAGTGGAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCag                                                                                           <  2:216814/141‑1 (MQ=255)

                                                         tGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAg‑agg                                                                                     <  1:602199/141‑1 (MQ=255)

                                                         tGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCACATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAg‑agg                                                                                     <  1:66082/141‑1 (MQ=255)

                                                            ccAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑aggagg                                                                                  >  1:539066/1‑141 (MQ=255)

                                                             cAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGgt                                                                                 <  2:417259/141‑1 (MQ=255)

                                                             cAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGgt                                                                                 <  2:175449/141‑1 (MQ=255)

                                                             cAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGgt                                                                                 <  2:175467/141‑1 (MQ=255)

                                                             cAATTAAGGGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGgt                                                                                 <  1:247119/141‑1 (MQ=255)

                                                             cAATAAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGgt                                                                                 <  2:25293/141‑1 (MQ=255)

                                                              aaTTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGgtg                                                                                <  2:156881/141‑1 (MQ=255)

                                                              aaTTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGgtg                                                                                <  1:12716/141‑1 (MQ=255)

                                                               aTTAAGGGGAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGgtgt                                                                               <  2:695065/141‑1 (MQ=255)

                                                                   aGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCGGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑CTAATTTAGACGCAGAG‑AGGAGGTGTA‑Taa                                                                          >  1:437386/1‑141 (MQ=255)

                                                                   aGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTGTA‑Taa                                                                          >  1:156165/1‑141 (MQ=255)

                                                                   aGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGAAA‑ACAAGTTTGACGGCGAG‑AGGAGGTGTa‑aaa                                                                          >  1:292766/1‑141 (MQ=255)

                                                                    gtgtAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGCGTA‑TCA‑g                                                                        >  2:100176/1‑141 (MQ=255)

                                                                    gtgtAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACCCAGAG‑CGGCGGTGTA‑AAA‑g                                                                        >  2:205334/1‑141 (MQ=255)

                                                                     tgtAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTGTA‑TAA‑g‑g                                                                      <  1:601325/141‑1 (MQ=255)

                                                                      gtAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGGGTA‑TAA‑g‑gg                                                                     >  1:246895/1‑140 (MQ=255)

                                                                           cGATTCAATCTATCGGCTAAAATCAAATAACTTATCAGAAGCAACTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTGTA‑TAA‑G‑G‑TGATGc                                                               >  1:423686/1‑141 (MQ=255)

                                                                           cGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTGTA‑TAA‑G‑G‑TGATGc                                                               >  1:386619/1‑141 (MQ=255)

                                                                           cGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCA‑TAAATGCAGACGCAGAG‑AGGAGGGGTA‑CAA‑G‑G‑TGATGc                                                               >  1:478470/1‑141 (MQ=255)

                                                                                 aaTCTTTCAGCTGAGATCATATCACTGATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTGTA‑TAA‑G‑G‑TGATGCTTAttt                                                         <  2:638542/141‑1 (MQ=255)

                                                                                 aaTCTATCAGCTAGAATCATATAACTTATCAGACGCAAGTGACTGTGCTTGTATATTTGCCGATGACATAATAGTAGAGTCGCCTATCTCTCAGGCG‑GCAATTTAGACGCAGAG‑AGGAGGTGTA‑TAA‑G‑G‑TGATGCTTAttt                                                         >  2:765749/1‑141 (MQ=255)

                                                                                    cTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCg                                                      <  1:534176/141‑1 (MQ=255)

                                                                                     tATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGt                                                     >  1:32723/1‑141 (MQ=255)

                                                                                             aGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCCcatca                                             >  2:455407/1‑141 (MQ=255)

                                                                                                  cATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGCTGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGTAGGTGTA‑TAA‑G‑G‑TGCTGCTTATTTTCGTTCACATCATAGCa                                        >  1:251606/1‑141 (MQ=255)

                                                                                                     ataACTTATCAGAAGCAAGAGACTGTGCTTGTATATTTGCCGATGATATAATAGCAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCa                                     >  1:503233/1‑141 (MQ=255)

                                                                                                        aCTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCAATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTGTA‑TAA‑G‑G‑TGATGCTTCTGTTCGTTCACGTCATAGCACCAGtc                                  >  1:377276/1‑141 (MQ=255)

                                                                                                         cTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGtca                                 >  2:643536/1‑141 (MQ=255)

                                                                                                                atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                         >  2:561683/15‑141 (MQ=255)

                                                                                                                atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                         <  1:117662/127‑1 (MQ=255)

                                                                                                                atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                         >  1:307206/15‑141 (MQ=255)

                                                                                                                 tcggaagtagatatgCTTGTCTATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCATTAC‑T‑T‑TGCTAATCAGTTTCGTTCACATCATAGCACCAGTCATCAGTGGCt                        >  1:471002/14‑141 (MQ=255)

                                                                                                                 tcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCt                        >  1:189291/14‑141 (MQ=255)

                                                                                                                  cggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCGATT‑‑GACGAAGAG‑AGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtg                       >  1:456350/13‑141 (MQ=255)

                                                                                                                   ggaagtagatacgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgt                      <  2:505844/129‑1 (MQ=255)

                                                                                                                    gaagttggtatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑CGGAGTTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCAGAGCACCAGTCATCAGTGGCtgtg                     <  1:255424/131‑1 (MQ=255)

                                                                                                                    gaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                     >  2:606465/11‑141 (MQ=255)

                                                                                                                    gaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCA‑TTA‑C‑TTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                     >  2:606190/11‑141 (MQ=255)

                                                                                                                    gaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGCCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                     <  2:134865/131‑1 (MQ=255)

                                                                                                                     aattagatatgCCTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCA‑TTA‑CTT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                    <  2:555817/132‑1 (MQ=255)

                                                                                                                     aagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCA‑TTA‑CTT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                    >  1:29408/10‑141 (MQ=255)

                                                                                                                     aaGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCCCCAGCCATCAGTGGCtgtg                     >  2:61050/1‑141 (MQ=255)

                                                                                                                     aaGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                     <  2:446337/141‑1 (MQ=255)

                                                                                                                      agtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGct                   >  1:613140/9‑140 (MQ=255)

                                                                                                                      actagatacgCTTGTATATTCACAGACGGTATAATAATAGAGTTGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCA‑TTA‑C‑TTTGCTAATCATTTTCGTTCCCATCATAGCACCAGTCATCAGTGGCTGTGct                   <  1:192797/132‑2 (MQ=255)

                                                                                                                      aGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                    <  1:742496/141‑1 (MQ=255)

                                                                                                                       gtagatatgCCTGTATACTCACAGATGGTACAATAATAGCGTCGCCCACCTCGCGTCCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                  <  1:21780/134‑3 (MQ=255)

                                                                                                                        tagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgt                 <  1:316784/135‑4 (MQ=255)

                                                                                                                        atgatatgattGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCA‑TTA‑C‑TTTGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgt                 <  2:156768/132‑4 (MQ=255)

                                                                                                                         agatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgtt                >  1:550046/6‑137 (MQ=255)

                                                                                                                          gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCA‑TTA‑CTT‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg               >  1:646252/5‑136 (MQ=255)

                                                                                                                          ccggtgCTTGGAGATTTGCCGATGATATAATAGTAGAGTAGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGCGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCCTCAGTGGCTGTGCCAtt                <  2:209532/138‑1 (MQ=255)

                                                                                                                          aatattcTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg               <  1:706627/135‑6 (MQ=255)

                                                                                                                          aCTGTGCTTGTATATTTGCCTATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGGAGAG‑AGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCAtt                <  2:315977/141‑1 (MQ=255)

                                                                                                                           atatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttgg              >  1:534432/4‑135 (MQ=255)

                                                                                                                           atatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttgc              <  1:315760/138‑7 (MQ=255)

                                                                                                                           atatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttgc              <  1:684803/138‑7 (MQ=255)

                                                                                                                            tgtgCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGc              <  1:60674/141‑1 (MQ=255)

                                                                                                                            tgtgCTTGTATATTCGCCGATGATATAATAGGAGAGTCGCCTATCTCTCCGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGc              <  2:28513/141‑1 (MQ=255)

                                                                                                                            tatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGAGCTGTTGCg             <  1:567487/139‑1 (MQ=255)

                                                                                                                            tatgCTTGTATATGCACAGATGGTAAAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCg             <  1:698596/139‑1 (MQ=255)

                                                                                                                            tatgCTTGTATACTCACAGAAGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCg             <  1:567610/139‑1 (MQ=255)

                                                                                                                             gtgCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGGGGCGGA‑CAA‑G‑C‑AGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATGGCg             >  1:177912/1‑141 (MQ=255)

                                                                                                                             gtgCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCg             >  2:402339/1‑141 (MQ=255)

                                                                                                                             gtgCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCT‑AAAGAAGCAAAAGTGAGCCG‑AGTGAACGTCGAGTA‑TAA‑G‑G‑TGATGCTTGTTTTCGTTCACATCAGAGCACCAGTCATCAGTGGCTGTACCATTGCg             >  1:370522/1‑141 (MQ=255)

                                                                                                                             atgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGt            <  1:105831/140‑1 (MQ=255)

                                                                                                                               gCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGtt           >  1:691625/1‑141 (MQ=255)

                                                                                                                                cTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatt         >  2:642691/1‑141 (MQ=255)

                                                                                                                                  gttattTTGGCCGATGATATAATAGTAGAGTCGCTTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTGTA‑TAA‑G‑T‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGttttt        <  2:395127/139‑1 (MQ=255)

                                                                                                                                   gTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattata      <  1:538346/141‑7 (MQ=255)

                                                                                                                                   gTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCA‑TTACT‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattata      <  2:558452/141‑7 (MQ=255)

                                                                                                                                      tatTTACAGATGGTGTAATAATAGAGTCGCCTATCTCTCAGGCG‑TCAATT‑‑GACGAAGAG‑AGGAGGTGCATTAC‑T‑T‑TGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatactt   <  1:198709/141‑7 (MQ=255)

                                                                                                                                        tttGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCA‑TCAATTTAGACGCAGAG‑ACACGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCACCAGTGACTGTTCCCTTGCGtttttttactt  >  2:174548/1‑137 (MQ=255)

TTGTCATAATAAAATCGCCTACTTTCTTAAATTAACAATATCTATTCTCATAGAATTTGTCCAATTAAGTGTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCG‑TCAATTTAGACGCAGAG‑AGGAGGTGTA‑TAA‑G‑G‑TGATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTA  >  CP000730/1929658‑1929934

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`