BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,929,870	A→C	64.4%	intergenic (‑953/‑215)	USA300HOU_1794 ← / → USA300HOU_1796	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,929,870	0	A	C	64.4%	232.3 / 162.3	191	intergenic (‑953/‑215)	USA300HOU_1794/USA300HOU_1796	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (34/34); new base C (59/63); total (94/97)
Fisher's exact test for biased strand distribution p-value = 8.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 192 total aligned reads displayed.

CGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAG  >  CP000730/1929733‑1930009

    tCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GATGCTTAt                                                                                                                                      <  1:504010/141‑1 (MQ=255)

    tCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GATGCTTAt                                                                                                                                      <  2:820416/141‑1 (MQ=255)

    tCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GATGCTTAt                                                                                                                                      <  1:301731/141‑1 (MQ=255)

     cAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GATGCTTAtt                                                                                                                                     >  1:828033/1‑141 (MQ=255)

      aaTCTATCAGCTAGAATCATATAACTTATCAGCAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTACCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GATGCTTAttt                                                                                                                                    <  1:607812/141‑1 (MQ=255)

       aTCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGTGGTG‑TA‑TAA‑G‑G‑T‑GATGCTTAtttt                                                                                                                                   <  1:582428/141‑1 (MQ=255)

       aTCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GGTGCGTAtttt                                                                                                                                   >  1:324975/1‑141 (MQ=255)

                   gAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCAcatcat                                                                                                                       <  1:16187/141‑1 (MQ=255)

                    aaTCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTGTATTTGCCGATGATATAATAGTAGAGTAGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GATGCTTATTTTCGGTCACATCATa                                                                                                                      >  1:196502/1‑141 (MQ=255)

                            aaCTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGt                                                                                                              <  2:283593/141‑1 (MQ=255)

                                  tCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAg                                                                                                        >  2:459763/1‑141 (MQ=255)

                                     atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                                                                                                    <  2:616381/127‑1 (MQ=255)

                                     atcggaaggagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                                                                                                    <  1:240846/127‑1 (MQ=255)

                                      tcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCt                                                                                                   >  2:567492/14‑141 (MQ=255)

                                      tcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCt                                                                                                   >  1:259999/14‑141 (MQ=255)

                                      tcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCt                                                                                                   >  1:112339/14‑141 (MQ=255)

                                      tcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGAGCGT‑TAC‑T‑T‑T‑CCTCATCGCTTTCGTTCACATCATAGCACCAGTCATCAGCGGCt                                                                                                   >  1:616651/14‑141 (MQ=255)

                                      tcggaaggagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCCCATCATAGCACCAGTCATCAGTGGCt                                                                                                   >  2:199155/14‑141 (MQ=255)

                                        ggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgt                                                                                                 <  1:343615/130‑1 (MQ=255)

                                        ggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgt                                                                                                 <  2:336666/130‑1 (MQ=255)

                                          aagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                                                                                               <  2:638240/132‑1 (MQ=255)

                                            gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGGCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑C‑TTT‑GCTAATCATTTTCGTTCACATGATAGCACCAGTCATCATTGGCTGTGctg                                                                                             >  1:515352/8‑139 (MQ=255)

                                              gACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATATTACGAGGCATCAGTGGCTGTGCCAt                                                                                            <  2:646762/141‑1 (MQ=255)

                                              gACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCAt                                                                                            <  1:691294/141‑1 (MQ=255)

                                              agatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgtt                                                                                           >  1:559027/6‑137 (MQ=255)

                                              agatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑CTT‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgtt                                                                                           <  2:245269/136‑5 (MQ=255)

                                               gatatgCTTGTATTTTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg                                                                                          >  1:572585/5‑136 (MQ=255)

                                               gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg                                                                                          <  1:882442/137‑6 (MQ=255)

                                                 tgtgCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCCTAGCACCAGTCATCAGTGGCTGTGCCATTGc                                                                                         <  2:779727/141‑1 (MQ=255)

                                                 tgtgCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATCGCACCAGTCATCAGTGGCTGTGCCATTGc                                                                                         >  1:122779/1‑141 (MQ=255)

                                                 tatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCg                                                                                        >  1:208309/3‑141 (MQ=255)

                                                 tatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACAAGTCATCATTGGCTGTGCTGTTGCg                                                                                        >  2:226741/3‑141 (MQ=255)

                                                 gatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTGTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GCTCAGCATTTTCGTTCACATCCTAGCACCAGTCATCAGTGGCTGTGCTGTTGCg                                                                                        <  2:56404/139‑1 (MQ=255)

                                                  atgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGt                                                                                       <  1:637536/140‑1 (MQ=255)

                                                     cTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatt                                                                                    <  1:110514/141‑4 (MQ=255)

                                                     cTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatt                                                                                    <  1:383506/141‑4 (MQ=255)

                                                     cTTGTATATTCACAGATGGTATAATAATAGAGGCGCCTATCTTTGAGGCGTCAAGTT‑‑ACGAAGAGAGGAGGTG‑CA‑TTA‑CTT‑T‑GCTAATCATTTTCGTTCACATCATAGCACCTGTCATCAATGGCTGTGCTGTTGCGtatt                                                                                    >  1:447839/1‑141 (MQ=255)

                                                      ttGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatta                                                                                   <  1:251866/141‑5 (MQ=255)

                                                      ttGTATATTCACAGAAGGTATAATATGAGAGTCGCCTAGCTCTCAGGCTTCAAGTT‑‑ACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGGCAGCAGTGGCTGTGCTGTTGCGtagta                                                                                   <  2:166106/141‑5 (MQ=255)

                                                       tGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGttttat                                                                                  <  2:631480/141‑3 (MQ=255)

                                                         tataTTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatac                                                                                <  2:307152/141‑5 (MQ=255)

                                                         tataTTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatac                                                                                <  1:194973/141‑8 (MQ=255)

                                                             ttcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GCTAATCATTTTCAATCACATCATAGCACCAGTCATCACTGTCAGTGCTGTTGCGTATTATACTTAt                                                                            >  2:145154/7‑141 (MQ=255)

                                                               cacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTg                                                                          >  2:115255/5‑141 (MQ=255)

                                                                 cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc                                                                        <  2:914863/139‑1 (MQ=255)

                                                                 cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc                                                                        <  2:104301/139‑1 (MQ=255)

                                                                  cGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGc                                                                        <  1:723492/141‑1 (MQ=255)

                                                                  agatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCCATT‑‑GACGAAGGGAGGAGGTG‑CA‑TTT‑CTT‑T‑GCTAGTCATTTTCGTTGACATCGTAGGACCGGTCGTCGGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt                                                                       <  2:667089/140‑1 (MQ=255)

                                                                   gatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtt                                                                      <  2:559027/141‑2 (MQ=255)

                                                                     tgatATAATAGTAGAGTCGCCTATCTCTCAGGAGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTaa                                                                     >  1:653723/1‑141 (MQ=255)

                                                                      ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                                   >  1:104876/3‑141 (MQ=255)

                                                                      ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                                   >  1:609994/3‑141 (MQ=255)

                                                                      ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                                   >  2:404668/3‑141 (MQ=255)

                                                                      ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATGATACTTATTGGCTTAGt                                                                   <  1:219423/139‑1 (MQ=255)

                                                                      ggtacaataatAGAGTCGCCTATCTCTCAGCCGTCAATT‑‑GACGAAGAGAGGAGATG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAga                                                                   >  2:554136/6‑140 (MQ=255)

                                                                        tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaa                                                                 >  2:731600/1‑140 (MQ=255)

                                                                        tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaa                                                                 >  2:133234/1‑140 (MQ=255)

                                                                        tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTGAGTaa                                                                 >  2:698714/1‑140 (MQ=255)

                                                                         ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaa                                                                >  1:777417/1‑139 (MQ=255)

                                                                         ataataGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGGTAAGtca                                                                 >  2:578842/1‑139 (MQ=255)

                                                                          taataGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGa                                                                >  2:65728/1‑141 (MQ=255)

                                                                          taataGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGa                                                                >  1:289535/1‑141 (MQ=255)

                                                                          taataGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGa                                                                <  1:230257/141‑1 (MQ=255)

                                                                            ataataGGGGCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑C‑T‑TCGCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                                             <  2:601457/141‑1 (MQ=255)

                                                                             taatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAAGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCa                                                            <  2:852428/138‑1 (MQ=255)

                                                                              aatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑C‑GTT‑GCTAATCATTTTCGTTCACATCATAGCACCAGTTATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaa                                                           <  2:671873/139‑1 (MQ=255)

                                                                                 agagTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATa                                                         <  1:846460/141‑1 (MQ=255)

                                                                                 agagTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaa                                                        <  1:790465/141‑4 (MQ=255)

                                                                                   agTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACa                                                       >  1:521234/1‑141 (MQ=255)

                                                                                   agTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaat                                                      <  1:677652/141‑6 (MQ=255)

                                                                                    gTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCGTTTGCGTTTTATACTTATTGGCTTAGTAAACGCaacaaata                                                     >  1:189030/1‑135 (MQ=255)

                                                                                    gTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑AATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACACaacaaata                                                     >  2:597656/1‑135 (MQ=255)

                                                                                      cGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACaaaa                                                    <  1:239109/141‑1 (MQ=255)

                                                                                       gCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAAt                                                   >  2:432488/1‑141 (MQ=255)

                                                                                       gCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑AA‑TAA‑G‑G‑T‑GATGGTTATTGTCTTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAAt                                                   <  2:277341/141‑1 (MQ=255)

                                                                                       gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑C‑TTT‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                                  <  2:623208/141‑10 (MQ=255)

                                                                                       gCCTATCTCTCAGGCGTAAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑CTT‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                                  <  1:878105/141‑10 (MQ=255)

                                                                                          tATCTCTCAGGCTTCAATT‑‑GGCGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTTGTTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaat                                               <  2:365251/141‑13 (MQ=255)

                                                                                          tATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaat                                               >  2:530364/1‑129 (MQ=255)

                                                                                          tATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑CTT‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaat                                               <  2:59483/141‑13 (MQ=255)

                                                                                          tATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTA‑CTT‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCGGTTGCGTATTATACTTATTGGCTGAGTAGACACaacaaataattaaa                                               >  1:141676/1‑129 (MQ=255)

                                                                                           ttctctcAGGCGTCAATT‑‑GACGGAGAGAGGAGTTG‑CA‑TTA‑CTT‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                                              <  1:98123/140‑14 (MQ=255)

                                                                                           aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                                              <  1:819367/141‑14 (MQ=255)

                                                                                           aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                                              >  1:364646/1‑128 (MQ=255)

                                                                                           aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTCTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                                              >  1:606306/1‑128 (MQ=255)

                                                                                           aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CA‑TTACT‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaatcattaatg                                              >  1:59342/1‑128 (MQ=255)

                                                                                            tctctcAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCTTTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTg                                              >  1:802774/1‑141 (MQ=255)

                                                                                            tctctcAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattattag                                             <  2:790341/141‑15 (MQ=255)

                                                                                            tctctcAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaatag                                             <  1:728110/141‑15 (MQ=255)

                                                                                            tctctcAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaatag                                             >  2:808231/1‑127 (MQ=255)

                                                                                            tctctcAGGCGTCAATT‑‑GACGAAGAGAGGAGGTG‑CATTAC‑T‑T‑T‑GCTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATAATTATTGGCTGAGTAAACCCaacaactaattaatag                                             >  1:790687/1‑127 (MQ=255)

                                                                                            tccctcggacGTCAGTTTAAGCGAAGAGAGGAGGCG‑TA‑TAA‑G‑G‑T‑GATGCGGATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTGAGGGGTAAGGAGACGCAAGACAAAATAGGTg                                              >  2:873909/10‑141 (MQ=255)

                                                                                             ctctcAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GATGATCACGCGGGTTCACGTAATAGTCCCAGGCATCAGTGGCTGTGCCATTGCGTTTTTTGCTGATTTGCTAAGTAGACGCAATACAAAATAGGTGa                                             >  1:901647/1‑141 (MQ=255)

                                                                                                                   cAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATaaaaa                       <  2:714883/141‑1 (MQ=255)

                                                                                                                             gtg‑tA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACt             <  1:753867/141‑1 (MQ=255)

                                                                                                                              tg‑tA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAATTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTa            >  2:736959/1‑141 (MQ=255)

                                                                                                                                 ta‑taA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTAcc          <  2:338221/141‑1 (MQ=255)

                                                                                                                                  a‑taA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCg         >  1:84796/1‑141 (MQ=255)

                                                                                                                                      a‑G‑G‑T‑GATGCTTATTTACGTTCACATCTTAGCACCTGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTCGTCGCAATACAAAATGGGTGACATATAGCCGCACCAATATAAATCCCCTCACTACCGCaa      <  1:551202/141‑1 (MQ=255)

                                                                                                                                      a‑G‑G‑T‑CATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCCGTGCCACTGCGTTTTTTTCTTATTGGCTACGTAGACGCAATACAACATCGGTCACATATAGCCGCACCAATACAAACCCCCTCACTACCGCaa      <  2:477213/141‑1 (MQ=255)

CGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTG‑TA‑TAA‑G‑G‑T‑GATGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCAAATAG  >  CP000730/1929733‑1930009

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`