BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,161	T→C	32.8%	I131V (ATT→GTT)	tpn ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,161	0	T	C	32.8%	197.0 / 198.3	242	I131V (ATT→GTT)	tpn	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base T (70/92); new base C (37/42); total (108/134)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01

Only 100 of 249 total aligned reads displayed.

TTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGT‑ACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTC  >  CP000730/36022‑36301

tttcttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGT‑ACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAAtt                                                                                                                                             <  2:95753/141‑1 (MQ=255)

tttcttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGT‑ACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCAATGCTACAGTCCCTTAAGGTGCCTGATCTGTAAtt                                                                                                                                             <  1:89295/141‑1 (MQ=14)

 ttcttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGT‑ACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCCTTGAAGGGGCCTGATATGAAAttc                                                                                                                                            >  2:167059/1‑140 (MQ=255)

  tcttATTTTAATATTGCGGTTATCTTGCTCAATGAGGTTATTCAGATATTTCGATGT‑ACAATGGCAGTCAGGTTTCAGTTTAAAAGCTTTGATTACTTTACCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTAc                                                                                                                                           <  2:160482/141‑1 (MQ=11)

   cttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGT‑ACAATCACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGACGGTGCCTGAGTTGtacctacc                                                                                                                                          >  2:212722/1‑141 (MQ=11)

    ttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGT‑ACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCt                                                                                                                                         >  1:335960/1‑141 (MQ=255)

    ttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGT‑ACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTTAGTACCt                                                                                                                                         >  2:318072/1‑141 (MQ=255)

       cTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGT‑ACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCtttt                                                                                                                                      >  1:318484/1‑141 (MQ=25)

           aaTATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGT‑ACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCACTTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAgg                                                                                                                                  >  1:69213/1‑141 (MQ=11)

             tatGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGT‑ACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGtt                                                                                                                                >  1:2031/1‑141 (MQ=255)

               ttaCGGTGATTTTGGTTAATGAGGTTATTCAGATATTTCGATGT‑ACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCCGTATTTACCTTTTGAGGTTTa                                                                                                                              <  1:354290/139‑1 (MQ=11)

               tGACGGTGATTTTGCCCAATGAGGTTATATTTTTATTTGGATGTGACTA‑GCAACTCAGGGTTAAGTTTAAAAGCTTTATTTACTTTAACCATTGCCACCTTCGTTGAAGGTGCCTGAGCTGTAATTACCTTTTGAGGTTTa                                                                                                                              <  1:365966/141‑1 (MQ=2)

                 aCGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGT‑ACAATGGCAGTCCGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGCTCTGTAATTACCTTTTTAGGTTTAcc                                                                                                                            >  2:45555/1‑141 (MQ=11)

                 aCGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGT‑ACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAcc                                                                                                                            >  1:244402/1‑141 (MQ=25)

                 aCGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGT‑ACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTGTAACCATTGCTACCGTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAcc                                                                                                                            <  1:217246/141‑1 (MQ=255)

                 aCGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGT‑ACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTCAGCCTTAcc                                                                                                                            >  2:173595/1‑141 (MQ=11)

                 aCGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGT‑ACAATGAAAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTTCCTGATCTGTAACTACATTTTGGGGCTTAca                                                                                                                            >  1:148839/1‑140 (MQ=11)

                   ggTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGT‑ACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTACTTACCTTTTGAGGTTTACCaa                                                                                                                          <  2:187568/141‑1 (MQ=12)

                    gTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGT‑ACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCaaa                                                                                                                         >  1:219345/1‑141 (MQ=17)

                    gTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGT‑ACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCaaa                                                                                                                         <  1:131275/141‑1 (MQ=11)

                    gTGATCCTCCTCAATGAGGTTATTCAGATACTTCGATGT‑ACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCaaa                                                                                                                         <  2:76034/141‑1 (MQ=11)

                      taTCTTGCTCAATGAGGTTATTCAGATATTTCGATGT‑ACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTGAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAAtt                                                                                                                       <  1:67471/140‑1 (MQ=255)

                      gATCTTGCTCAATGAGGTTATTCAGATATTTCGATGT‑ACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAAGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAAtt                                                                                                                       >  2:154242/1‑141 (MQ=12)

                        tCTTGCTCAATGAGGTTATTCAGATATTTCGATGT‑ACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGt                                                                                                                     >  2:294986/1‑141 (MQ=12)

                        tCTTGCTCAATGAGGTTATTCAGATATTTCGATGT‑ACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCGATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGt                                                                                                                     >  2:267196/1‑141 (MQ=11)

                        tCTTGCTCAATGAGGTTATTCAGATATTTCGATGT‑ACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGt                                                                                                                     <  1:227327/141‑1 (MQ=11)

                           tGCTCAATGAGGTTATTCAGATATTTCGATGT‑ACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTa                                                                                                                  <  1:186193/141‑1 (MQ=32)

                            gCTCAATGAGGTTATTCAGATATTTCGATGT‑ACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTaa                                                                                                                 <  2:185073/141‑1 (MQ=17)

                             cTCAATGAGGTTATTCAGATATTTCGATGT‑ACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAAt                                                                                                                <  1:321417/141‑1 (MQ=12)

                              tCAATGAGGGTATTCAGTTATTTCGATGT‑ACAATGGCAGTCAGGTTTAAGTTTAGAAGCTTTAATTAGTTTAACCATTGCTTCCTTCGTTGAAGGTGTCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATg                                                                                                               <  2:70321/141‑1 (MQ=11)

                                aaTGAGGTTATTCAGATATTTCGATGT‑ACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACTACATAGTTTAATgag                                                                                                             >  2:66785/1‑141 (MQ=11)

                                aaTGAGGTTATTCAGATATTTCGATGT‑ACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATgag                                                                                                             >  2:276688/1‑141 (MQ=255)

                                  tGAGGTTATTCAGATATTTCGATGT‑ACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGAc                                                                                                           <  1:105459/141‑1 (MQ=11)

                                   gAGGTTATTCAGATATTTCGATGT‑ACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACg                                                                                                          <  2:261188/141‑1 (MQ=17)

                                   gAGGTTATTCAGATAATTCGATGT‑ACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGAACTGTAATTACCTTTTAAGGTGTACCAAATTGTTTAATGAGACg                                                                                                          <  2:79980/141‑1 (MQ=11)

                                    gggTTATTCAGATATTTCGATGT‑ACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGt                                                                                                         <  2:234086/140‑1 (MQ=12)

                                    aGGTTATTCAGATATTTCGATGT‑ACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGt                                                                                                         <  2:301587/141‑1 (MQ=17)

                                    aGGTTATTCAGATATTTCGATGT‑ACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGt                                                                                                         <  1:253416/141‑1 (MQ=11)

                                    aGGTTATTCAGATATTTCGATGT‑ACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGt                                                                                                         >  1:56821/1‑141 (MQ=11)

                                     ggTTATTCAGATATTTCGATGT‑ACAATGCCAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGtt                                                                                                        <  1:201173/141‑1 (MQ=11)

                                      gTTATTCAGATATTTCGATGT‑ACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGttt                                                                                                       <  2:10176/141‑1 (MQ=17)

                                      gTTATTCAGATATTTCGATGT‑ACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGttt                                                                                                       <  1:195762/141‑1 (MQ=17)

                                       ttATTCAGATATTTCGATGT‑ACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTg                                                                                                      <  1:317116/141‑1 (MQ=11)

                                       ttATTCAGATATTTCGATGG‑ACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTg                                                                                                      >  1:197112/1‑141 (MQ=12)

                                       ttATTCAAATATTTCGATTC‑ACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTg                                                                                                      <  2:249908/141‑1 (MQ=11)

                                        tATTCAGATATTTCGATGT‑ACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGa                                                                                                     <  1:222428/141‑1 (MQ=12)

                                        tATTCAGATATTTCGATGT‑ACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGa                                                                                                     >  2:193166/1‑141 (MQ=12)

                                        tATTCAGATATTTCGATGT‑ACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGa                                                                                                     <  1:237799/141‑1 (MQ=11)

                                         aTTCAGATATTTCGATGT‑ACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGAt                                                                                                    >  2:206500/1‑141 (MQ=255)

                                                   ttCGATGT‑ACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCatat                                                                                          <  1:305075/141‑1 (MQ=17)

                                                     cGATGT‑ACAATGGCAGTCAGGGTTAAGTTTAAAAGCTTTAATTACTTTAACCAATCCTCCCATCGTTTAATGTGCCTGGTCTGAAATGACCTTTTGAGGTTTACCAAATTGTTTAATGAGATGTTTGATAAACGCATATGc                                                                                        <  1:198090/141‑1 (MQ=11)

                                                       aTGT‑ACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTg                                                                                      <  2:327609/141‑1 (MQ=17)

                                                       aTGT‑ACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCta                                                                                      >  1:213549/1‑140 (MQ=17)

                                                       aTGT‑ACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTg                                                                                      >  2:309093/1‑141 (MQ=32)

                                                       aTGT‑ACAATGACAGCCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTg                                                                                      <  2:355741/141‑1 (MQ=11)

                                                            aCAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATg                                                                                  <  2:282106/141‑1 (MQ=17)

                                                            aCAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATg                                                                                  >  2:131837/1‑141 (MQ=255)

                                                             cAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGa                                                                                 >  1:341190/1‑141 (MQ=255)

                                                              aaggACAGTCAGGTTTAAGTTGAAAAGCTTTAATTATGTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGAt                                                                                <  2:118688/138‑1 (MQ=11)

                                                              aaTGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGAt                                                                                <  1:262994/141‑1 (MQ=11)

                                                                                                   tttAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGTTTACGCAACAAAATATCTAatgtatg                                           >  1:308586/1‑141 (MQ=11)

                                                                                                   tttAGCCATTGCCGCCTGCGTTCAAGGTGCCCGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAatgtatg                                           <  2:352855/141‑1 (MQ=11)

                                                                                                   tttAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAatgtatg                                           <  1:230869/141‑1 (MQ=11)

                                                                                                    ttAGCCTTTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTCCCTTTTGAGGCTTACCAAATTGTTTAATGAGCCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAatgtatgt                                          <  2:27449/141‑1 (MQ=11)

                                                                                                       gCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTccc                                       >  2:23965/1‑141 (MQ=255)

                                                                                                           ttGCTACCTTCGTTTAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTg                                   >  1:311952/1‑141 (MQ=17)

                                                                                                           ttGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTg                                   >  1:339999/1‑141 (MQ=32)

                                                                                                            tGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGGTTGGTAAACGCATATGCTGAGTGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGc                                  >  2:93491/1‑141 (MQ=12)

                                                                                                             gCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTgta                                 <  1:270394/141‑3 (MQ=17)

                                                                                                              cTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTAt                                >  2:344917/1‑141 (MQ=17)

                                                                                                               tgccTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATc                               <  2:229210/139‑1 (MQ=255)

                                                                                                               tgccTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATc                               >  1:361569/3‑141 (MQ=255)

                                                                                                               tACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATc                               >  2:368216/1‑141 (MQ=32)

                                                                                                                 ccTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCTCCCTCACGATAATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCaa                             >  1:176971/1‑141 (MQ=11)

                                                                                                                 ccTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCaa                             >  1:124576/1‑141 (MQ=17)

                                                                                                                 ccTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTACGTCCCTCTGCATCaa                             <  2:113540/141‑1 (MQ=17)

                                                                                                                 ccTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCaa                             <  1:114375/141‑1 (MQ=12)

                                                                                                                  cTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCAGGTGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAAt                            >  1:271833/1‑141 (MQ=12)

                                                                                                                      gTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGcc                        >  1:153596/1‑140 (MQ=17)

                                                                                                                        tGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTCATGTATGTCCCTCTGTATCAATGGCACg                      >  1:177785/1‑141 (MQ=17)

                                                                                                                        tGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACg                      >  1:304815/1‑141 (MQ=32)

                                                                                                                         gAATGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTTTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGa                     <  2:335265/141‑1 (MQ=17)

                                                                                                                            ggTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGatat                  >  1:239467/1‑141 (MQ=12)

                                                                                                                            ggTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGatat                  >  2:28415/1‑141 (MQ=255)

                                                                                                                             gTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGAtata                 <  2:197112/141‑1 (MQ=17)

                                                                                                                              tGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCGATGGCACGATATaa                >  2:58826/1‑141 (MQ=17)

                                                                                                                              tGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGatttca                >  1:100983/1‑137 (MQ=17)

                                                                                                                                ccTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAAt              >  1:44724/1‑141 (MQ=17)

                                                                                                                                ccTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAAt              >  1:140675/1‑141 (MQ=32)

                                                                                                                                 cTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATa             <  2:143411/141‑1 (MQ=32)

                                                                                                                                 cTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTCATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGGAAGCGCCTCTGTATCAATGGCACGATATAAATa             <  2:247167/141‑1 (MQ=11)

                                                                                                                                  tGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATGTAAATAg            >  1:292805/1‑141 (MQ=255)

                                                                                                                                    aTCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTTCCTCTGTATCAATGGCACGATATAAATAGCt          <  1:34266/141‑1 (MQ=17)

                                                                                                                                    aTCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTGCGCAACCAAATATCTAATGTATGTCCCTCTGTATCGATGGCAGGATAAAAATAGCt          >  2:297106/1‑141 (MQ=11)

                                                                                                                                     tCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTc         <  2:26437/141‑1 (MQ=32)

                                                                                                                                       tGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCa       <  1:152401/141‑1 (MQ=32)

                                                                                                                                         tAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATTTAAATAGCTCCAtt     >  1:298374/1‑141 (MQ=17)

                                                                                                                                         tAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCAtt     <  1:206500/141‑1 (MQ=12)

                                                                                                                                           aTTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCAtttt   >  2:302972/1‑141 (MQ=17)

                                                                                                                                           aTTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCAtttt   >  1:326591/1‑141 (MQ=17)

TTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGT‑ACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTC  >  CP000730/36022‑36301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`