BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,174	T→C	34.2%	K126K (AAA→AAG)	tpn ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,174	0	T	C	34.2%	178.8 / 205.0	244	K126K (AAA→AAG)	tpn	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base T (73/86); new base C (35/49); total (108/136)
Fisher's exact test for biased strand distribution p-value = 5.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.72e-01

Only 100 of 245 total aligned reads displayed.

TATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGT‑ACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTG  >  CP000730/36035‑36312

tatGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGT‑ACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGtt                                                                                                                                           >  1:2031/1‑141 (MQ=255)

  tGACGGTGATTTTGCCCAATGAGGTTATATTTTTATTTGGATGTGACTA‑GCAACTCAGGGTTAAGTTTAAAAGCTTTATTTACTTTAACCATTGCCACCTTCGTTGAAGGTGCCTGAGCTGTAATTACCTTTTGAGGTTTa                                                                                                                                         <  1:365966/141‑1 (MQ=2)

     cGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGT‑ACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCa                                                                                                                                      <  2:64606/141‑1 (MQ=11)

      ggTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGT‑ACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGTTACTTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCaa                                                                                                                                     <  2:204886/141‑1 (MQ=255)

       gTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGT‑ACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCaaa                                                                                                                                    <  1:149182/141‑1 (MQ=17)

       gTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGT‑ACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCaaa                                                                                                                                    <  1:131275/141‑1 (MQ=11)

         gATCTTGCTCAATGAGGTTATTCAGATATTTCGATGT‑ACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAAGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAAtt                                                                                                                                  >  2:154242/1‑141 (MQ=12)

         gATCTTGCTCAATGAGGTTATTCAGATATTTCGATGT‑ACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAAtt                                                                                                                                  <  1:345793/141‑1 (MQ=32)

           tCTTGCTCAATGAGGTTATTCAGATATTTCGATGT‑ACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGt                                                                                                                                >  1:325456/1‑141 (MQ=17)

           tCTTGCTCAATGAGGTTATTCAGATATTTCGATGT‑ACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGt                                                                                                                                >  2:294986/1‑141 (MQ=12)

           tCTTGCTCAATGAGGTTATTCAGATATTTCGATGT‑ACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCGATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGt                                                                                                                                >  2:267196/1‑141 (MQ=11)

           tCTTGCTCAATGAGGTTATTCAGATATTTCGATGT‑ACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATGTTAGCCATTGCAGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGt                                                                                                                                >  2:279057/1‑141 (MQ=11)

            cTTGCTCAATGAGGTTATTCAGATATTTCGATGT‑ACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGtt                                                                                                                               >  1:134885/1‑141 (MQ=17)

               gCTCAATGAGGTTATTCAGATATTTCGATGT‑ACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTaa                                                                                                                            <  2:185073/141‑1 (MQ=17)

               gCTCAATGAGGTTATTCAGATATTTCAATGT‑ACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTaa                                                                                                                            <  1:179493/141‑1 (MQ=12)

                cTCAATGAGGTTATTCAGATATTTCGATGT‑ACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAAt                                                                                                                           <  2:190543/141‑1 (MQ=17)

                cTCAATGAGGTTATTCAGATATTTCGATGT‑ACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAAt                                                                                                                           <  2:175132/141‑1 (MQ=17)

                 tCAATGAGGTTATTCAGATATTTCGATGT‑ACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATg                                                                                                                          <  2:76787/141‑1 (MQ=32)

                 tCAATGAGGGTATTCAGTTATTTCGATGT‑ACAATGGCAGTCAGGTTTAAGTTTAGAAGCTTTAATTAGTTTAACCATTGCTTCCTTCGTTGAAGGTGTCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATg                                                                                                                          <  2:70321/141‑1 (MQ=11)

                     tGAGGTTATTCAGATATTTCGATGT‑ACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGAc                                                                                                                      <  1:105459/141‑1 (MQ=11)

                       gggTTATTCAGATATTTCGATGT‑ACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGt                                                                                                                    <  2:234086/140‑1 (MQ=12)

                       aGGTTATTCAGATATTTCGATGT‑ACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGt                                                                                                                    >  1:318977/1‑141 (MQ=12)

                       aGGTTATTCAGATATTTCGATGT‑ACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGt                                                                                                                    <  1:253416/141‑1 (MQ=11)

                         gTTATTCAGATATTTCGATGT‑ACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGttt                                                                                                                  <  2:10176/141‑1 (MQ=17)

                          ttATTCAGATATTTCGATGT‑ACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTg                                                                                                                 <  1:317116/141‑1 (MQ=11)

                           tATTCAGATATTTCGATGT‑ACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGa                                                                                                                >  2:193166/1‑141 (MQ=12)

                                   tatTTCGATGT‑ACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCa                                                                                                        >  1:216872/1‑141 (MQ=12)

                                      ttCGATGT‑ACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCatat                                                                                                     >  1:22247/1‑141 (MQ=17)

                                       tCGATGT‑ACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATg                                                                                                    >  1:340904/1‑141 (MQ=255)

                                        cGATGT‑ACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACTAAATTGTTTAATGAGACGTTTGATAAACGCATATGc                                                                                                   >  1:217858/1‑141 (MQ=255)

                                          aTGT‑ACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTg                                                                                                 <  2:327609/141‑1 (MQ=17)

                                          aTGT‑ACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCta                                                                                                 >  1:213549/1‑140 (MQ=17)

                                            gT‑ACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGaa                                                                                               <  1:142530/141‑1 (MQ=17)

                                             t‑ACAATGACTGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTCGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAAt                                                                                              <  2:97606/141‑1 (MQ=11)

                                                cAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGa                                                                                            >  1:153818/1‑141 (MQ=255)

                                                cAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGattca                                                                                            <  2:4006/141‑5 (MQ=17)

                                                 aaggACAGTCAGGTTTAAGTTGAAAAGCTTTAATTATGTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGAt                                                                                           <  2:118688/138‑1 (MQ=11)

                                                 aaTGGCAGTCATGTTTAAGTTTAACAGCTTTAATTACTTTAACCATTGCTAGTTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGTTTACCAAATTTTTTAATGAGACGTTTGATAAACTCATATGCTGAATGAt                                                                                           <  2:131135/141‑1 (MQ=11)

                                                 aaTGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGAt                                                                                           <  1:262994/141‑1 (MQ=11)

                                                                           gcgttAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGTTCTGTAATTACCTTTTGAGGTTTACGAAATTTTTTAAGGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCATCCAAata                                                                 <  1:234769/138‑1 (MQ=1)

                                                                                      tttAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGTTTACGCAACAAAATATCTAatgtatg                                                      >  1:308586/1‑141 (MQ=11)

                                                                                      tttAGCCATTGCCGCCTGCGTTCAAGGTGCCCGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAatgtatg                                                      <  2:352855/141‑1 (MQ=11)

                                                                                      ttgaaccATGGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAatgtatg                                                      <  1:46607/136‑1 (MQ=2)

                                                                                       ttAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAatgtatgt                                                     <  1:196825/141‑1 (MQ=9)

                                                                                       ttAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAatgtatgt                                                     <  2:309208/141‑1 (MQ=14)

                                                                                         aaccATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTcc                                                   <  2:304595/139‑1 (MQ=17)

                                                                                          gCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTccc                                                  >  2:23965/1‑141 (MQ=255)

                                                                                          accATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTCCCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACTAAATATCTAATGTATGTccc                                                  >  1:197192/2‑141 (MQ=17)

                                                                                          accATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTccc                                                  >  2:262239/2‑141 (MQ=17)

                                                                                            ctttGTTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCctc                                                <  2:9319/139‑1 (MQ=17)

                                                                                            cATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAATCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCctc                                                <  1:315077/141‑1 (MQ=17)

                                                                                            cATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTGGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCctc                                                <  1:32939/141‑1 (MQ=17)

                                                                                              ttGCTACTTTCGTTGAAGGTGGCTGATCTGTAATTACCTTTTGAGGTTTGCCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTg                                              <  2:225728/141‑1 (MQ=12)

                                                                                              ttGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTg                                              >  1:299310/1‑141 (MQ=32)

                                                                                              ttGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTg                                              >  1:339999/1‑141 (MQ=32)

                                                                                              ttGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCGCGTTGCTTACGCAACCAAATATCTAATGTATGTCCctctt                                              >  1:69117/1‑140 (MQ=17)

                                                                                              ttGCTACCTTCGTTGAAGGTGCCTGAACTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTg                                              <  1:228350/141‑1 (MQ=17)

                                                                                               tgctgcCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTgt                                             <  1:283439/141‑2 (MQ=12)

                                                                                                gCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTgta                                            <  1:270394/141‑3 (MQ=17)

                                                                                                 cTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGGCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATGTAATGTATGTCACTCTGTAt                                           >  2:238671/1‑141 (MQ=255)

                                                                                                 cTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTAt                                           >  2:344917/1‑141 (MQ=17)

                                                                                                  tgccTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATc                                          >  1:361569/3‑141 (MQ=255)

                                                                                                  tACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATc                                          >  2:368216/1‑141 (MQ=32)

                                                                                                    ccTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCTCCCTCACGATAATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCaa                                        >  1:176971/1‑141 (MQ=11)

                                                                                                    ccTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCaa                                        >  2:25143/1‑141 (MQ=17)

                                                                                                    ccTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCaa                                        >  1:305335/1‑141 (MQ=12)

                                                                                                    ccTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCaa                                        <  1:240015/141‑1 (MQ=255)

                                                                                                     cTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCAGGTGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAAt                                       >  1:271833/1‑141 (MQ=12)

                                                                                                      ttCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATg                                      >  1:193814/1‑141 (MQ=17)

                                                                                                         gTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCa                                   <  1:153302/141‑1 (MQ=12)

                                                                                                          ttGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCAc                                  >  1:119019/1‑141 (MQ=12)

                                                                                                           tGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACg                                 >  1:304815/1‑141 (MQ=32)

                                                                                                           tGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCCCTGTATCAATGGCACg                                 >  2:173091/1‑141 (MQ=17)

                                                                                                               ggTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGatat                             >  2:28415/1‑141 (MQ=255)

                                                                                                                gTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGAtata                            <  2:197112/141‑1 (MQ=17)

                                                                                                                gTGCCTGATCTGTAATCACTATTTGAGGTTCACCAAATTGTTAAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGACCCTCTGTATCAATGGCACGAtata                            <  1:238671/141‑1 (MQ=11)

                                                                                                                 tGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGatttca                           >  1:100983/1‑137 (MQ=17)

                                                                                                                    cTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATa                        <  2:354763/141‑1 (MQ=17)

                                                                                                                    cTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATa                        <  2:143411/141‑1 (MQ=32)

                                                                                                                    cTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTCATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGGAAGCGCCTCTGTATCAATGGCACGATATAAATa                        <  2:247167/141‑1 (MQ=11)

                                                                                                                    cTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAAGGACACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATa                        <  2:323816/141‑1 (MQ=12)

                                                                                                                     tGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATGTAAATAg                       >  1:292805/1‑141 (MQ=255)

                                                                                                                       aTCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTTCCTCTGTATCAATGGCACGATATAAATAGCt                     <  1:34266/141‑1 (MQ=17)

                                                                                                                       aTCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTGCGCAACCAAATATCTAATGTATGTCCCTCTGTATCGATGGCAGGATAAAAATAGCt                     >  2:297106/1‑141 (MQ=11)

                                                                                                                          tGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCa                  <  1:220395/141‑1 (MQ=17)

                                                                                                                           ttAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCAt                 <  2:271817/140‑1 (MQ=17)

                                                                                                                            tAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCAtt                <  1:206500/141‑1 (MQ=12)

                                                                                                                             aactACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCTCTCTGTATCAATGGGACGATATAAATAGCACCAttt               >  1:83130/4‑141 (MQ=11)

                                                                                                                             aactACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCAttt               <  1:267196/138‑1 (MQ=12)

                                                                                                                              aTTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCAtttt              >  2:302972/1‑141 (MQ=17)

                                                                                                                               ttACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTc             >  1:199765/1‑141 (MQ=32)

                                                                                                                                tACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTcc            <  2:276089/141‑1 (MQ=32)

                                                                                                                                 aCCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCt           <  2:56821/141‑1 (MQ=12)

                                                                                                                                 aCCTTTGGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCt           <  2:335960/141‑1 (MQ=12)

                                                                                                                                  ccTTTTGAGGTTTACCAAATTGTTTAATTAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCtt          <  1:240599/141‑1 (MQ=17)

                                                                                                                                  ccTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCCTCAATGGCACGATATAACTCGCTCCGCTTTCAtt          >  2:248395/1‑141 (MQ=11)

                                                                                                                                  ccTTTTGAGGGTTACTAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCtt          <  1:113727/141‑1 (MQ=17)

                                                                                                                                      ttGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTAt      >  1:63236/1‑141 (MQ=32)

                                                                                                                                       tGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAAGGTATGTCCCTCTGTATCAATGGCACGATATAAATAGACCCATTTTCATTTTaat     >  2:213637/1‑139 (MQ=11)

                                                                                                                                        gAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTAttt    >  1:115258/1‑141 (MQ=17)

TATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGT‑ACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTG  >  CP000730/36035‑36312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`