BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	35,913	C→G	32.8%	S213S (TCG→TCC)	tpn ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	35,913	0	C	G	32.8%	149.6 / 139.5	138	S213S (TCG→TCC)	tpn	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base C (39/53); new base G (25/20); total (65/73)
Fisher's exact test for biased strand distribution p-value = 2.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.37e-01

Only 100 of 144 total aligned reads displayed.

CTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCT‑AGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTC  >  CP000730/35773‑36053

cTTGAACAAGATTTAAAATAAAATTTTGATCTAATAAGAATAGAAGGTTCTGTTGCAAAGTAAAATAATATAGCTTACCTCTAATTTCTCATGTCAGTGTTCGCTTAACTTGCT‑AGCATGATGCTAATTTCGTGGCATGGc                                                                                                                                                <  2:158653/141‑1 (MQ=17)

cTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCT‑AGCATGATGCTAATTTCGTGGCATGGc                                                                                                                                                <  2:208903/141‑1 (MQ=255)

cTTAAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCGGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCT‑AGCATGATGCTAATTTCGTGGCATGGc                                                                                                                                                <  2:243140/141‑1 (MQ=255)

     aCAAGAGCTAAAATAAAATTGTCATCTAATAAAAATAGACAGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATCTATCATTGGAGGGTTCGCTTAACTTGCT‑AGCATGATGCTAATTTCGTGGCATGGCGaaaa                                                                                                                                           <  1:311465/141‑1 (MQ=17)

         gagCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCT‑AGCATGATTCTAATTTCGTGGCATGGCGAAAATCCg                                                                                                                                       >  2:310540/1‑141 (MQ=255)

           gCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCT‑AGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTa                                                                                                                                     >  2:213002/1‑141 (MQ=255)

             taaaataaaaTTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCT‑AGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGa                                                                                                                                   >  2:187007/1‑141 (MQ=255)

                        tgtgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCT‑AGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                                                                                                        <  2:108939/141‑1 (MQ=255)

                          tgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCT‑AGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCt                                                                                                                      >  1:60335/1‑141 (MQ=255)

                          tattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCT‑AGCATGATGCTAGTTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                                                                                                                    <  2:342107/136‑1 (MQ=11)

                          tattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCT‑AGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                                                                                                                    <  1:81528/136‑1 (MQ=11)

                           attttaataaAAAT‑GATGGTTCTGTTGCAAAG‑AAAAAAATATAGCTAATCACTAATATATCATGTCAGTGGACGTTTAACTTGCT‑AGCATGATGCTAATTTCGTGGCATGGCGAAAAACCGTAGATATGAAGAGACCTGCg                                                                                                                   >  2:365966/5‑141 (MQ=11)

                            ttttaataaAAAT‑GCTGGTTCTGTTGCAAAGT‑AAAAAATATAGCCAACCACTAATATATCCTGTCAGTGTTCGTTTAACTTGCT‑AGCATGATGCTAATTGCGTTGCATGGCGAAAATCCGGAGATCTGAAGAGACCTGCgg                                                                                                                  <  1:321997/138‑1 (MQ=11)

                            aTCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCT‑AGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                                                                                                                    <  2:10051/141‑1 (MQ=255)

                            aTCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCT‑AGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                                                                                                                    <  2:186990/141‑1 (MQ=255)

                              ttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCT‑AGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGtt                                                                                                                <  2:340933/140‑1 (MQ=11)

                                                                          taatcccTAATATATCATGTCAGTGTTCGTTTAACTTGCT‑AGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGT‑AAATACATTCAATACCTTTTAAAGt                                                                    <  1:102662/135‑1 (MQ=1)

                                                                             ccgcTAAGTTACCATGTCAGTGTTCGCTTAACTTGCT‑AGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGAGCGGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGT‑AAATACACTCAATACCTTTTAAAGTAtt                                                                 <  2:1647/138‑1 (MQ=1)

                                                                                              gtgtgtTGGTTTAACTTGCG‑AGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATTTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATAc                                                <  2:50975/139‑1 (MQ=11)

                                                                                              cAGTGTTCGCTTAACTTGCT‑AGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATAc                                                <  1:75650/141‑1 (MQ=11)

                                                                                               aGTGTTCGTTTAACTTGCT‑AGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACt                                               >  1:325465/1‑141 (MQ=255)

                                                                                                gttttCGTTTTACTTGCT‑AGCATGATGCTAATTTCGAGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACtt                                              <  1:220734/138‑1 (MQ=11)

                                                                                                gtgtTCGTTGAAGTTGCT‑AGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACtt                                              <  2:241365/141‑1 (MQ=11)

                                                                                                 tgtTCGTTTAACTTGCT‑AGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACttt                                             >  1:249908/1‑141 (MQ=255)

                                                                                                 tgtTCGTTTAACTTGCT‑AGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGA‑GAACACGGTCAAGACCTTTTAAAGTATTCTTTGCCGTATTGATACttt                                             >  1:28804/1‑141 (MQ=11)

                                                                                                 tgtTCGTTTAACTTGCT‑AGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACttt                                             >  2:325923/1‑141 (MQ=17)

                                                                                                  gtTCGTTTAACTTGCT‑AGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTg                                            >  2:340462/1‑141 (MQ=12)

                                                                                                  gtTCGCTTAACTTGCTCGGCATGGT‑TTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTg                                            >  2:265240/1‑141 (MQ=11)

                                                                                                   ttCGCTTAACTTGCT‑AGCATGATGCTAATTTCGTGGCATGGCGAACATCCGCAGGTGTGAAAAGGCCTGCGGTTCTTATTATATCAGA‑CAC‑CAAAACACTCCATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa                                           >  2:113727/1‑141 (MQ=11)

                                                                                                   ttCGCTTAACTTGCT‑AGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGCTCTGCAGAGACCTGCGGTTCTTTTTGTAT‑AGAGCGT‑AACTACACTCAATACCTTTTAAAGTATTATTTTCTGTATGTATACTTTgg                                           >  2:240568/1‑140 (MQ=11)

                                                                                                    tCGTTTAACTTGCT‑AGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGAt                                          >  1:74246/1‑141 (MQ=17)

                                                                                                     cgtttAACTTGCT‑AGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATa                                         >  2:139994/4‑141 (MQ=255)

                                                                                                     cgtttAACTTGCT‑AGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATa                                         <  1:193857/138‑1 (MQ=17)

                                                                                                      gtttAACTTTCT‑AGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTCATAT‑AGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATCGATACTTTGATat                                        <  2:157458/139‑2 (MQ=11)

                                                                                                      gtttAACTTGCT‑AGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATat                                        >  1:118688/3‑140 (MQ=255)

                                                                                                      gCTTAACTTGCT‑AGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGT‑AAATACACTCCATGCCTGTTAAAGTATTCTTTGCTGTATTGATACTTTGATAc                                        >  1:287963/1‑141 (MQ=255)

                                                                                                      gCTTAACTTGCT‑AGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAc                                        <  2:365020/141‑1 (MQ=17)

                                                                                                      gCTTAACTTGCT‑AGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAc                                        <  2:49348/141‑1 (MQ=17)

                                                                                                       tttAACTTGCT‑AGCATGATGCTAATTTCGTGGCATGGCGACAATATGTAGATTTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGT‑AAATACACTCAATACCGTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAcc                                       <  2:132230/140‑1 (MQ=11)

                                                                                                       tttAACTTGCT‑AGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAcc                                       <  2:351589/140‑1 (MQ=17)

                                                                                                       cTTAACTTGCT‑AGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGTCCTGCGGTTCTTTTTATAT‑AGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAcc                                       <  2:229755/141‑1 (MQ=17)

                                                                                                         tAACTTGCC‑AGCATGTTGCTAATTTCGTGGCATGTGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑ATCGCGT‑AAATACGTTCAATACCTTTTAACGTATTCTTTGCCTTATTGATACTTTGATATCtt                                     <  2:164765/141‑1 (MQ=11)

                                                                                                          aaCTTGCT‑AGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTg                                    >  2:183620/1‑141 (MQ=255)

                                                                                                          aaCTTGCT‑AGCATGATGCTAACTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGATCTTATGATAG‑AGAGAGT‑AGATCCTCTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTg                                    <  1:87772/141‑1 (MQ=11)

                                                                                                           aCTTGCT‑AGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGt                                   >  1:282719/1‑141 (MQ=32)

                                                                                                           aCTTGCT‑AGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGt                                   >  1:234086/1‑141 (MQ=17)

                                                                                                            cTTGCT‑AGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATTTCTTGtc                                  >  2:214842/1‑141 (MQ=11)

                                                                                                            cTTGCT‑AGCATGATGCTAATGTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtc                                  <  1:67282/141‑1 (MQ=17)

                                                                                                             ttGCT‑AGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtct                                 <  2:320869/141‑1 (MQ=17)

                                                                                                             ttGCT‑AGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtct                                 <  2:64471/141‑1 (MQ=17)

                                                                                                              tGCT‑AGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtctt                                >  1:6175/1‑141 (MQ=255)

                                                                                                              tGCT‑AGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctt                                <  1:333939/141‑1 (MQ=17)

                                                                                                               gCT‑AGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATATTGtcttt                               >  1:159523/1‑141 (MQ=11)

                                                                                                               gCT‑AGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtcttt                               <  2:262409/141‑1 (MQ=17)

                                                                                                               gCT‑AGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtcttt                               <  2:106598/141‑1 (MQ=17)

                                                                                                               gCT‑AGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACATTGtcttt                               >  1:10176/1‑141 (MQ=17)

                                                                                                               gCT‑AGCATGATGCTAATTTCGTGGCATGGCGAAAATCACTCCAACTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtcttt                               <  1:288150/141‑1 (MQ=11)

                                                                                                                cT‑AGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtatttc                              >  2:247793/1‑141 (MQ=11)

                                                                                                                 t‑AGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttct                             <  1:322165/141‑1 (MQ=32)

                                                                                                                 t‑AGCATGATGCTAATTTCGTGGCATGAGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGT‑AAATAGATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtctttct                             <  1:227971/141‑1 (MQ=11)

                                                                                                                   aGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATTTTGtctttctt                            >  2:118383/1‑141 (MQ=11)

                                                                                                                   aGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGTTGTATTGATACTTTGATACCTTGcctttatt                            >  2:220395/1‑141 (MQ=255)

                                                                                                                   aGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtcttactt                            >  2:250530/1‑141 (MQ=17)

                                                                                                                    gCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGGTACTTGGATATCTTGtctttctgt                           >  1:27449/1‑139 (MQ=11)

                                                                                                                     cATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATGGATACGTTGATATCTTGTCTTTCTTAc                          >  1:307587/1‑141 (MQ=11)

                                                                                                                       tgatgCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACtt                        >  1:374363/1‑141 (MQ=11)

                                                                                                                       tgatgCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACtt                        >  1:354009/1‑141 (MQ=255)

                                                                                                                         atgCTAATTTCGTGGCATGGAGAAAATCCGTAGATCTGAAGAGAGCTACGGTTCTTTTGATAT‑AGAGCGT‑AAATACATTCAATACCTTTTAAGGTATTCTTTGCCGTATTGATCATTTGATATCTCGTCTTTCTTACTTTa                      >  1:226146/1‑141 (MQ=11)

                                                                                                                          tgCTAATTTCGTGGCATGGCGCAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATTT‑AGAGCGG‑AAATACACCCAATACCTTTTAAAGTATTCTTTGCTGTTTTGATACTTTGATACCTTGTCTTTCTTACTTTaa                     >  1:131354/1‑141 (MQ=11)

                                                                                                                          tgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTaa                     <  1:75992/141‑1 (MQ=21)

                                                                                                                          tgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTCCTTACTTTaa                     <  1:270660/141‑1 (MQ=25)

                                                                                                                           gCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGGCCTGCGGTTCTTTTTATAT‑AGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATCCCTTGTCTTTTTTACTTTAat                    >  1:110179/1‑141 (MQ=255)

                                                                                                                            cTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAata                   <  1:40514/141‑1 (MQ=255)

                                                                                                                            cTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAata                   >  2:269327/1‑141 (MQ=21)

                                                                                                                             tAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCGTACTTTAatat                  >  1:232312/1‑141 (MQ=11)

                                                                                                                              aaTTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATg                 >  2:230225/1‑141 (MQ=35)

                                                                                                                              aaTTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATg                 >  1:358758/1‑141 (MQ=21)

                                                                                                                               aTTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGa                >  1:166263/1‑141 (MQ=11)

                                                                                                                               aTTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTAAGGCTATTTTTATAT‑AGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGa                >  1:352855/1‑141 (MQ=11)

                                                                                                                                tttCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGAc               <  1:359891/141‑1 (MQ=11)

                                                                                                                                 ttCGTGGCATGGGGAAAATCCGTAGAGCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGT‑AAATACATTCAATACCTTTTAAAGTATGCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACg              <  1:127175/141‑1 (MQ=11)

                                                                                                                                 ttCGTGGCATGGGCAAAGTCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTTCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACg              <  1:25851/141‑1 (MQ=11)

                                                                                                                                  tCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACgg             <  1:289429/141‑1 (MQ=255)

                                                                                                                                  tCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACgg             <  1:48683/141‑1 (MQ=38)

                                                                                                                                  tCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACgg             <  1:340462/141‑1 (MQ=38)

                                                                                                                                   cGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGt            <  1:266827/141‑1 (MQ=255)

                                                                                                                                    gTGGCATGGGGAAAATCCGTAGATCTGAAGAGACTTACTGTTCTTTTTATAT‑AGAGCGT‑AAATACATTCAATACCTATTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTg           <  1:101840/141‑1 (MQ=14)

                                                                                                                                    gTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTg           <  1:217799/141‑1 (MQ=255)

                                                                                                                                    gTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTg           <  1:239946/141‑1 (MQ=255)

                                                                                                                                     tGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGa          >  2:303568/1‑141 (MQ=255)

                                                                                                                                     gggCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGa          <  2:334242/140‑1 (MQ=255)

                                                                                                                                      ggCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAt         >  2:234769/1‑141 (MQ=38)

                                                                                                                                      ggCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAt         >  1:65540/1‑141 (MQ=255)

                                                                                                                                      ggCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAt         >  1:43998/1‑141 (MQ=255)

                                                                                                                                      ggCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAt         >  2:85693/1‑141 (MQ=37)

                                                                                                                                      ggCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCCTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAt         <  1:248921/141‑1 (MQ=255)

                                                                                                                                       gCATGGCGAAAATCCGTAGATCTGCAGAGACCTGCGGTTTTTTTTGT‑C‑AGATCCTGAAATACACTCAATACCGTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATc        <  1:175336/141‑1 (MQ=14)

                                                                                                                                       gCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATc        <  1:210740/141‑1 (MQ=255)

                                                                                                                                          tGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTg     <  1:47451/141‑1 (MQ=255)

                                                                                                                                             cGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTc  <  2:237843/141‑1 (MQ=255)

CTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCT‑AGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTC  >  CP000730/35773‑36053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`