Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	2,610,079	(T)7→8	coding (447/795 nt)	USA300HOU_2473 ←	lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,610,072	1	.	T	80.3%	199.2 / 47.2	71	coding (454/795 nt)	USA300HOU_2473	lipoprotein
Reads supporting (aligned to +/- strand):  ref base . (4/10);  new base T (20/37);  total (24/47)
Fisher's exact test for biased strand distribution p-value = 7.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01

GACATTATAATCATCATTATTCAATTGATATTTTGCCGAATAACTTGGTACATTAGGATTATATGAAATATCACCATCTTTATAATCATTAATATCTTTAAAGTTGCCATATTGTACAAAGAACTTAAAGTTTTCAATCTC‑‑TTTTTTTAACTTGTCATTCGGTAGTGGCTTCGTTGGTATGATTTTATTATGTTCCATTTTTACCGGATACCTTTTTTCATCATCCTTTGGTCTGCCGTTACTGTCATCTGTCATTTCACTTATAAAATAATACCC  >  CP000730/2609932‑2610207                                                                                                                                              |                                                                                                                                         gACATTATAATCATCATTATTCAATTGATATTTTGCCGAATAACTTGGTACATTAGGATTATATGAAATATCACCATCTTTATAATCATTAATATCTTTAAAGTTGCCATATTGTACAAAGAACTTAAAGTTTTCAAtctc                                                                                                                                           >  2:107109/1‑141 (MQ=255)  aCATTATAATCATCATTATTCAATTGATATTTTGCCGAATAACTTGGTACATTAGGATTATATGAAATATCACCATCTTTATAATCATTAATATCTTTAAAGTTGCCATATTGTACAAAGAACTTAAAGTTTTCAATctc‑‑t                                                                                                                                        <  2:17880/141‑1 (MQ=255)  aCATTATAATCATCATTATTCAATTGATATTTTGCCGAATAACTTGGTACATTAGGATTATATGAAATATCACCATCTTTATAATCATTAATATCTTTAAAGTTGCCATATTGTACAAAGAACTTAAAGTTTTCAATctc‑‑t                                                                                                                                        <  1:711239/141‑1 (MQ=255)   cATTATAATCATCATTATTCAATTGATATTTTGCCGAATAACTTGGTACATTAGGATTATATGAAATATCACCATCTTTATAATCATTAATATCTTTAAAGTTGCCATATTGTACAAAGAACTTAAAGTTTTCAATCTC‑‑tt                                                                                                                                       >  1:305529/1‑141 (MQ=255)    aTTATAATCATAATTATTCGATTGATATTTTGCCGAATAACTTGGTACATTAGGATTATATGAAATATCACCATCTTTATAATCATTAATATCTTTAAAGTTGCCATATTGTACAAAGAACTTAAAGTTTTCAATCTC‑‑ttt                                                                                                                                      <  2:916078/141‑1 (MQ=255)     ttATAATCATCATTATTCAATTGATATTTTGCCGAATAACTTGGTACATTAGGATTATATGAAATATCACCATCTTTATAATCATTAATATCTTTAAAGTTGCCATATTGTACAAAGAACTTAAAGTTTTCAATCTC‑‑tttt                                                                                                                                     >  1:818730/1‑141 (MQ=255)     ttATAATCATCATTATTCAATTGATATTTTGCCGAATAACTTGGTACATTAGGATTATATGAAATATCACCATCTTTATAATCATTAATATCTTTAAAGTTGCCATATTGTACAAAGAACTTAAAGTTTTCAATCTC‑‑tttt                                                                                                                                     >  2:883417/1‑141 (MQ=255)     ttATAATCATCATTATTCAATTGATATTTTGCCGAATAACTTGGTACATTAGGATTATATGAAATATCACCATCTTTATAATCATTAATATCTTTAAAGTTGCCATATTGTACAAAGAACTTAAAGTCTCCAATCTC‑‑tttt                                                                                                                                     >  1:652397/1‑141 (MQ=255)       ataATCATCATTATTCAATTGATATTTTGCCGAATAACTTGGTACATTAGGATTATATGAAATATCACCATCTTTATAATCATTAATATCTTTAAAGTTGCCATATTGTACAAAGAACTTAAAGTTTTCAATCTC‑‑tttttt                                                                                                                                   >  2:42140/1‑141 (MQ=255)         aaTCATCATTATTCAATTGATATTTTGCCGAATAACTTGGTACATTAGGATTATATGAAATATCACCATCTTTATAATCATTAATATCTTTAAAGTTGCCATATTGTACAAAGAACTTAAAGTTTTCAATCTC‑‑tttttttt                                                                                                                                 <  1:560640/141‑2 (MQ=255)         aaTCATCATTATTCAATTGATATTTTGCCGAATAACTTGGTACATTAGGATTATATGAAATATCACCATCTTTATAATCATTAATATCTTTAAAGTTGCCATATTGTACAAAGAACTTAAAGTTTTCAATCTC‑‑tttttttt                                                                                                                                 >  1:282096/1‑140 (MQ=255)         aaTCATCATTATTCAATTGATATTTTGCCGAATAACTTGGTACATTAGGATTATATGAAATATCACCATCTTTATAATCATTAATATCTTTAAAGTTGCCATATTGTACAAAGAACTTAAAGTTTTCAATCTC‑‑tttttttt                                                                                                                                 <  1:560595/141‑2 (MQ=255)         aaTCATCATTATTCAATTGATATTTTGCCGAATAACTTGGTACATTAGGATTATATGAAATATCACCATCTTTATAATCATTAATATCTTTAAAGTTGCCATATTGTACAAAGAACTTAAAGTTTTCAATCTC‑‑tttttttt                                                                                                                                 >  2:641875/1‑140 (MQ=255)         aaTCATCATTATTCAATTGATATTTTGCCGAATAACTTGGTACATTAGGATTATATGAAATATCACCATCTTTATAATCATTAATATCTTTAAAGTTGCCATATTGTACAAAGAACTTAAAGTTTTCAATCTC‑‑tttttttt                                                                                                                                 >  2:914640/1‑140 (MQ=255)          atcatcATTATTCAATTGATATTTTGCCGAATAACTTGGTACATTAGGATTATATGAAATATCACCATCTTTATAATCATTAATATCTTTAAAGTTGCCATATTGTACAAAGAACTTAAAGTTTTCAATCTC‑‑tttttttta                                                                                                                                >  2:112491/1‑139 (MQ=255)            catcatTATTCAATTGATATTTTGCCGAATAACTTGGTACATTAGGATTATATGAAATATCACCATCTTTATAATCATTAATATCTTTAAAGTTGCCATATTGTACAAAGAACTTAAAGTTTTCAATCTC‑‑ttttttttaac                                                                                                                              <  2:355897/141‑5 (MQ=255)            catcatTATTCAATTGATATTTTGCCGAATAACTTGGTACATTAGGATTATATGAAATATCACCATCTTTATAATCATTAATATCTTTAAAGTTGCCATATTGTACAAAGAACTTAAAGTTTTCAATCTC‑‑ttttttttaac                                                                                                                              <  2:517752/141‑5 (MQ=255)               catTATTCAATTGATATTTTGCCGAATAACTTGGTACATTAGGATTATATGAAATATCACCATCTTTATAATCATTAATATCTTTAAAGTTGCCATATTGTACAAAGAACTTAAAGTTTTCAATCTCT‑TTTTTTTAACTTg                                                                                                                            >  1:521862/1‑141 (MQ=255)                   tttCAATTGATATTTTGCCGAATAACTTGGTACATTAGGATTATATGAAATATCACCATCTTTATAATCATTAATATCTTTAAAGTTGCCATATTGTACAAAGAACTTAAAGTTTTCAATCTCTTTTTTTTTAACTTGTCa                                                                                                                         <  2:538710/140‑1 (MQ=255)                       aaTTGATATTTTGCCGAATAACTTGGTACATTAGGATTATATGAAATATCACCATCTTTATAATCATTAATATCTTTAAAGTTGCCATATTGTACAAAGAACTTAAAGTTTTCAATCTC‑‑TTTTTTTAACTTGTCATTCGGt                                                                                                                   <  2:326490/141‑1 (MQ=255)                        aTTGATATTTTGCCGAATAACTTGGTACATTAGGATTATATGAAATATCACCATCTTTATAATCATTAATATCTTTAAAGTTGCCATATTGTACAAAGAACTTAAAGTTTTCAATCTCT‑TTTTTTTAACTTGTCATTCGGt                                                                                                                   <  1:251302/141‑1 (MQ=255)                            atatTTTGCCGAATAACTTGGTACATTAGGATTATATGAAATATCACCATCTTTATAATCATTAATATCTTTAAAGTTGCCATATTGTACAAAGAACTTAAAGTTTTCAATCTCT‑TTTTTTTAACTTGTCATTCGGTAGTg                                                                                                               <  1:1009618/141‑1 (MQ=255)                              atTTTGCCGAATAACTTGGTACATTAGGATTATATGAAATATCACCATCTTTATAATCATTAATATCTTTAAAGTTGCCATATTGTACAAAGAACTTAAAGTTTTCAATCTCT‑TTTTTTTAACTTGTCATTCGGTAGTGGc                                                                                                             >  1:937/1‑141 (MQ=255)                              atTTTGCCGAATAACTTGGTACATTAGGATTATATGAAATATCACCATCTTTATAATCATTAATATCTTTAAAGTTGCCATATTGTACAAAGAACTTAAAGTTTTCAATCTCT‑TTTTTTTAACTTGTCATTCGGTAGTGGc                                                                                                             >  1:2602/1‑141 (MQ=255)                               ttttGCCGAATAACTTGGTACATTAGGATTATATGAAATATCACCATCTTTATAATCATTAATATCTTTAAAGTTGCCATATTGTACAAAGAACTTAAAGTTTTCAATCTCTTTTTTTTTAACTTGTCATTCGGTAGTGGc                                                                                                             <  1:734589/141‑1 (MQ=255)                               ttttGCCGAATAACTTGGTACATTAGGATTATATGAAATATCACCATCTTTATAATCATTAATATCTTTAAAGTTGCCATATTGTACAAAGAACTTAAAGTTTTCAATCTCT‑TTTTTTTAACTTGTCATTCGGTAGTGGCt                                                                                                            <  1:652083/141‑1 (MQ=255)                                tttGCCGAATAACTTGGTACATTAGGATTATATGAAATATCACCATCTTTATAATCATTAATATCTTTAAAGTTGCCATATTGTACAAAGAACTTAAAGTTTTCAATCTC‑‑TTTTTTTAACTTGTCATTCGGTAGTGGCTTc                                                                                                          >  1:932966/1‑141 (MQ=255)                                 ttGCCGAATAACTTGGTACATTAGGATTATATGAAATATCACCATCTTTATAATCATTAATATCTTTAAAGTTGCCATATTGTACAAAGAACTTAAAGTTTTCAATCTCT‑TTTTTTTAACTTGTCATTCGGTAGTGGCTTc                                                                                                          >  1:993256/1‑141 (MQ=255)                                    ccGAATAACTTGGTACATTAGGATTATATGAAATATCACCATCTTTATAATCATTAATATCTTTAAAGTTGCCATATTGTACAAAGAACTTAAAGTTTTCAATCTCT‑TTTTTTTAACTTGTCATTCGGTAGTGGCTTCGtt                                                                                                       >  1:209734/1‑141 (MQ=255)                                       aaTAACTTGGTACATTAGGATTATATGAAATATCACCATCTTTATAATCATTAATATCTTTAAAGTTGCCATATTGTACAAAGAACTTAAAGTTTTCAATCTCT‑TTTTTTTAACTTGTCATTCGGTAGTGGCTTCGTTGGt                                                                                                    <  2:752337/141‑1 (MQ=255)                                         tAACTTGGTACATTAGGATTATATGAAATATCACCATCTTTATAATCATTAATATCTTTAAAGTTGCCATATTGTACAAAGAACTTAAAGTTTTCAATCTCT‑TTTTTTTAACTTGTCATTCGGTAGTGGCTTCGTTGGTAt                                                                                                  <  1:421799/141‑1 (MQ=255)                                         tAACTTGGTACATTAGGATTATATGAAAAATCACCATCTTTATAATCATTAATATCTTTAAAGTTGCCATATTGTACAAAGAACTTAAAGTTTTCAATCTCT‑TTTTTTTAANTTGTCATTCGGTNGTGGCTTCGTTGGTAt                                                                                                  <  1:875300/141‑1 (MQ=255)                                          aaCTTGGTACATTAGGATTATATGAAATATCACCATCTTTATAATCATTAATATCTTTAAAGTTGCCATATTGTACAAAGAACTTAAAGTTTTCAATCTCT‑TTTTTTTAACTTGTCATTCGGTAGTGGCTTCGTTGGTATg                                                                                                 >  2:132191/1‑141 (MQ=255)                                          aaCTTGGTACAATAGGATTATATGAAATATCAGCATCTTTATAATCATTAATATCTTTAAAGCTGCCATATTGTACAAAGAACTTAAAGTTTTCAATCTCT‑TTTTTTTAACTTGTCATTTGGAAGTGGCTTCGTTGGGAtt                                                                                                 >  2:375980/1‑140 (MQ=17)                                            cTTGGTACATTAGGATTATATGAAATATCACCATCTTTATAATCATTAATATCTTTAAAGTTGCCATATTGTACAAAGAACTTAAAGTTTTCAATCTCT‑TTTTTTTAACTTGTCATTCGGTAGTGGCTTCGTTGGTATGAt                                                                                               <  1:826667/141‑1 (MQ=255)                                             ttGGTACATTAGGATTATATGAAATATCACCATCTTTATAATCATTAATATCTTTAAAGTTGCCATATTGTACAAAGAACTTAAAGTTTTCAATCTC‑‑TTTTTTTAACTTGTCATTCGGTAGTGGCTTCGTTGGTATGAttt                                                                                             <  2:548716/141‑1 (MQ=255)                                              tGGTACATTAGGATTATATGAAATATCACCATCTTTATAATCATTAATATCTTTAAAGTTGCCATATTGTACAAAGAACTTAAAGTTTTCAATCTCT‑TTTTTTTAACTTGTCATTCGGTAGTGGCTTCg                                                                                                         >  1:832687/1‑129 (MQ=255)                                              tGGTACATTAGGATTATATGAAATATCACCATCTTTATAATCATTAATATCTTTAAAGTTGCCATATTGTACAAAGAACTTAAAGTTTTCAATCTCT‑TTTTTTTAACTTGTCATTCGGTAGTGGCTTCg                                                                                                         <  2:832687/129‑1 (MQ=255)                                               ggTACATTAGGATTATATGAAATATCACCATCTTTATAATCATTAATATCTTTAAAGTTGCCATATTGTACAAAGAACTTAAAGTTTTCAATCTCT‑TTTTTTTAACTTGTCATTCGGTAGTGGCTTCGTTGGTATGCtttt                                                                                            >  1:789110/1‑141 (MQ=255)                                                    aTTAGGATTATATGAAATATCACCATCTTTATAATCATTAATATCTTTAAAGTTGCCATATTGTACAAAGAACTTAAAGTTTTCAATCTCT‑TTTTTTTAACTTGTCATTCGGTAGTGGCTTCGTTGGTATGATTTtattat                                                                                       <  2:397788/141‑1 (MQ=255)                                                         gATTATATGAAATATCACCATCTTTATAATCATTAATATCTTTAAAGTTGCCATATTGTACAAAGAACTTAAAGTTTTCAATCTCT‑TTTTTTTAACTTGTCATTCGGTAGTGGCTTCGTTGGTATGATTTTATTATGTTcc                                                                                  <  2:460293/141‑1 (MQ=255)                                                         gATTATATGAAATATCACCATCCTTATAATCATTAATATCTTTAAAGTTGCCATATTGTACAAAGAACTTAAAGTTTTCAATCTCT‑TTTTTTTAACTTGTCATTCGGTAGTGGCTTCGTTGGTATGATTTTATTATGTTcc                                                                                  <  1:689909/141‑1 (MQ=255)                                                             atatGAAATATCACCATCTTTATAATCATTAATATCTTTAAAGTTGCCATATTGTACAAAGAACTTAAAGTTTTCAATCTCT‑TTTTTTTAACTTGTCATTCGGTAGTGGCTTCGTTGGTATGATTTTATTATGTTCCAttt                                                                              <  2:919630/141‑1 (MQ=255)                                                             atatGAAATATCACCATCTTTATAATCATTAATATCTTTAAAGTTGCCATATTGTACAAAGAACTTAAAGTTTTCAATCTC‑‑TTTTTTTAACTTGTCATTCGGTAGTGGCTTCGTTGGTATGATTTTATTATGTTCCAtttt                                                                             <  1:78713/141‑1 (MQ=255)                                                              tatGGATTATTACCATCTTTATAATCATTTATATCTTTAAAGTTGCCATATTGTACAAAGTACTGATAGTTTTCAATCTCT‑TTTTTTTTATTTGTCATTCGGTAGTGGCTTCGTTGGTATGAATTTATTATGTCACAtttc                                                                             <  2:727525/141‑2 (MQ=18)                                                              tatGAAATATCACCATCTTTATAATCATTAATATCTTTAAAGTTGCCATATTGTACAAAGAACTTAAAGTTTTCAATCTCT‑TTTTTTTAACTTGTCATTCGGTAGTGGCTTCGTTGGTATGATTTTATTATGTTCCAtttt                                                                             <  1:436088/141‑1 (MQ=255)                                                              tatGAAATATCACCATCTTTATAATCATTAATATCTTTAAAGTTGCCATATTGTACAAAGAACTTAAAGTTTTCAATCTCT‑TTTTTTTAACTTGTCATTCGGTAGTGGCTTCGTTGGTATGATTTTATTATGTTCCAtttt                                                                             <  2:424952/141‑1 (MQ=255)                                                                    atatCACCATCTTTATAATCATTAATATCTTTAAAGTTGCCATATTGTACAAAGAACTTAAAGTTTTCAATCTC‑‑TTTTTTTAACTTGTCATTCGGTAGTGGCTTCGTTGGTATGATTTTATTATGTTCCATTTTTACCGGa                                                                      <  1:907634/141‑1 (MQ=255)                                                                       tCACCATCTTTATAATCATTAATATCTTTAAAGTTGCCATATTGTACAAAGAACTTAAAGTTTTCAATCTC‑‑TTTTTTTAACTTGTCATTCGGTAGTGGCTTCGTTGGTATGATTTTATTATGTTCCATTTTTACCGGATAc                                                                   <  2:932966/141‑1 (MQ=255)                                                                        cACCATCTTTATAATCATTAATATCTTTAAAGTTGCCATATTGTACAAAGAACTTAAAGTTTTCAATCTCT‑TTTTTTTAACTTGTCATTCGGTAGTGGCTTCGTTGGTATGATTTTATTATGTTCCATTTTTACCGGATAc                                                                   >  2:355086/1‑141 (MQ=255)                                                                         aCCATCTTTATAATCATTAATATCTTTAAAGTTGCCATATTGTACAAAGAACTTAAAGTTTTCAATCTC‑‑TTTTTTTAACTTGTCATTCGGTAGTGGCTTCGTTGGTATGATTTTATTATGTTCCATTTTTACCGGATACCt                                                                 <  1:353252/141‑1 (MQ=255)                                                                            aTCTTTATAATCATTAATATCTTTAAAGTTGCCATATTGTACAAAGAACTTAAAGTTTTCAATCTCT‑TTTTTTTAACTTGTCATTCGGTAGTGGCTTCGTTGGTATGATTTTATTATGTTCCATTTTTACCGGATACCttt                                                               <  2:362390/141‑1 (MQ=255)                                                                              cTTTATAATCATTAATATCTTTAAAGTTGCCATATTGTACAAAGAACTTAAAGTTTTCAATCTCT‑TTTTTTTAACTTGTCATTCGGTAGTGGCTTCGTTGGTATGATTTTATTATGTTCCATTTTTACCGGATACCttttt                                                             >  2:88448/1‑141 (MQ=255)                                                                               tttATAATCATTAATATCTTTAAAGTTGCCATATTGTACAAAGAACTTATAGTTTTCAATCTCT‑TTTTTTTAATTTGTAATTTGGTAGTGGCTTCGTTTGTATGATTTTATTATTTTCCATTTTTTCTTGGTACCtttttt                                                            >  2:129718/1‑141 (MQ=18)                                                                                ttATAATCATTAATATCTTTAAAGTTGCCATATTGTACAAAGAACTTAAAGTTTTCAATCTCT‑TTTTTTTAACTTGTCATTCGGTAGTGGCTTCGTTGGTATGATTTTATTATGTTCCATTTTTACCGGATACCttttttt                                                           >  1:197890/1‑140 (MQ=255)                                                                                  ataATCATTAATATCTTTAAAGTTGCCATATTGTACAAAGAACTTAAAGTTTTCAATCTCT‑TTTTTTTAACTTGTCATTCGGTAGTGGCTTCGTTGGTATGATTTTATTATGTTCCATTTTTACCGGATACCTTTTTtcat                                                         <  1:46577/141‑1 (MQ=255)                                                                                  ataATCATTAATATCTTTAAAGTTGCCATATTGTACAAAGAACTTAAAGTTTTCAATCTCT‑TTTTTTTAACTTGTCATTCGGTAGTGGCTTCGTTGGTATGATTTTATTATGTTCCATTTTTACCGGATACCTTTTTtcat                                                         <  1:934148/141‑1 (MQ=255)                                                                                        aTTAATATCTTTAAAGTTGCCATATTGTACAAAGAACTTAAAGTTTTCAATCTC‑‑TTTTTTTAACTTGTCATTCGGTAGTGGCTTCGTTGGTATGATTTTATTATGTTCCATTTTTACCGGATTCCTTTTTTCATCATCCtt                                                  <  2:553296/141‑1 (MQ=255)                                                                                          tAATATCTTTAAAATAGCTATTTTGTACAAAGATCTTAAAGTTTTCAATCTCT‑TTTTTTTAACTTGTCATTCGGTAGTGGCTTCGTTCGTATGATTTTATTATGTTCCATTTTTACCGGATACCTTTTTTCATCATCCttt                                                 <  1:844264/141‑1 (MQ=25)                                                                                                 tttAAAGTTGCCATATTGTACAAAGAACTTAAAGTTTTCAATCTCT‑TTTTTTTAACTTGTCATTCGGTAGTGGCTTCGTTGGTATGATTTTATTATGTTCCATTTTTACCGGATACCTTTTTTCATCATCCTTTGGTCTGc                                          >  1:272671/1‑141 (MQ=255)                                                                                                 tttAAAGTTGCCATATTGTACAAAGAACTTAAAGTTTTCAATCTC‑‑TTTTTTTAACTTGTCATTCGGTAGTGGCTTCGTTGGTATGATTTTATTATGTTCCATTTTTACCGGATACCTTTTTTCATCATCCTTTGGTCTGcc                                         <  2:763047/141‑1 (MQ=255)                                                                                                  ttAAAGTTGCCATATTGTACAAAGAACTTAAAGTTTTCAATCTCT‑TTTTTTTAACTTGTCATTCGGTAGTGGCTTCGTTGGTATGATTTTATTATGTTCCATTTTTACCGGATACCTTTTTTCATCATCCTTTGGTCTGcc                                         >  1:482457/1‑141 (MQ=255)                                                                                                  ttAAAGTTGCCATATTGTACAAAGAACTTAAAGTTTTCAATCTCT‑TTTTTTTAACTTGTCATTCGGTAGTGGCTTCGTTGGTATGATTTTATTATGTTCCATTTTTACCGGATACCTTTTTTCATCATCCTTTGGTCTGcc                                         >  1:482452/1‑141 (MQ=255)                                                                                                      aGTTGCCATATTGTACAAAGAACTTAAAGTTTTCAATCTCT‑TTTTTTTAACTTGTCATTCGGTAGTGGCTTCGTTGGTATGATTTTATTATGTTCCATTTTTACCGGATACCTTTTTTCATCATCCTTTGGTCTGCCGTTa                                     <  1:410396/141‑1 (MQ=255)                                                                                                      aGTTGCCATATTGTACAAAGAACTTAAAGTTTTCAATCTCT‑TTTTTTTAACTTGTCATTCGGTAGTGGCTTCGTTGGTATGATTTTATTATGTTCCATTTTTACCGGATACCTTTTTTCATCATCCTTTGGTCTGCCGTTa                                     <  1:892851/141‑1 (MQ=255)                                                                                                      aGTTGCCATATTGTACAAAGAACTTAAAGTTTTCAATCTCT‑TTTTTTTAACTTGTCATTCGGTAGTGGCTTCGTTGGTATGATTTTATTATGTTCCATTTTTACCGGATACCTTTTTTCATCATCCTTTGGTCTGCCGTTa                                     <  1:440363/141‑1 (MQ=255)                                                                                                      aGTTGCCATATTGTACAAAGAACTTAAAGTTTTCAATCTCT‑TTTTTTTAACTTGTCATTCGGTAGTGGCTTCGTTGGTATGATTTTATTATGTTCCATTTTTACCGGATACCTTTTTTCATCATCCTTTGGTCTGCCGTTa                                     <  1:986817/141‑1 (MQ=255)                                                                                                      aGTTGCCATATTGTACAAAGAACTTAAAGTTTTCAATCTCT‑TTTTTTTAACTTGTCATTCGGTAGTGGCTTCGTTGGTATGATTTTATTATGTTCCATTTTTACCGGATACCTTTTTTCATCATCCTTTGGTCTGCCGTTa                                     <  1:986866/141‑1 (MQ=255)                                                                                                           ccATATTGTACAAAGAACTTAAAGTTTTCAATCTC‑‑TTTTTTTAACTTGTCATTCGGTAGTGGCTTCGTTGGTATGATTTTATTATGTTCCATTTTTACCGGATACCTTTTTTCATCATCCTTTGGTCTGCCGTTActgtca                               >  1:441983/1‑141 (MQ=255)                                                                                                            cATATTGTACAAAGAACTTAAAGTTTTCAATCTCT‑TTTTTTTAACTTGTCATTCGGTAGTGGCTTCGTTGGTATGATTTTATTATGTTCCATTTTTACCGGATACCTTTTTTCATCATCCTTTGGTCTGCCGTTActgtca                               <  2:484084/141‑1 (MQ=255)                                                                                                               atTGTACAAAGAACTTAAAGTTTTCAATCTCT‑TTTTTTTAACTTGTCATTCGGTAGTGGCTTCGTTGGTATGATTTTATTATGTTCCATTTTTACCGGATACCTTTTTTCATCATCCTTTGGTCTGCCGTTActgtcatct                            <  1:328028/141‑1 (MQ=255)                                                                                                               atTGTACAAAGAACTTAAAGTTTTCAATCTCT‑TTTTTTTAACTTGTCATTCGGTAGTGGCTTCGTTGGTATGATTTTATTATGTTCCATTTTTACCGGATACCTTTTTTCATCATCCTTTGGTCTGCCGTTActgtcatct                            <  1:1022126/141‑1 (MQ=255)                                                                                                               atTGTACAAAGAACTTAAAGTTTTCAATCTC‑‑TTTTTTTAACTTGTCATTCGGTAGTGGCTTCGTTGGTATGATTTTATTATGTTCCATTTTTACCGGATACCTTTTTTCATCATCCTTTGGTCTGCCGTTActgtcatctg                           <  2:1001464/141‑1 (MQ=255)                                                                                                                ttGTACAAAGAACTTAAAGTTTTCAATCTCT‑TTTTTTTAACTTGTCATTCGGTAGTGGCTTCGTTGGTATGATTTTATTATGTTCCATTTTTACCGGATACCTTTTTTCATCATCCTTTGGTCTGCCGTTActgtcatctg                           <  2:693572/141‑1 (MQ=255)                                                                                                                    aCAAAGAACTTAAAGTTTTCAATCTCT‑TTTTTTTAACTTGTCATTCGGTAGTGGCTTCGTTGGTATGATTTTATTATGTTCCATTTTTACCGGATACCTTTTTTCATCATCCTTTGGTCTGCCGTTActgtcatctgtcat                       <  1:352227/141‑1 (MQ=255)                                                                                                                     caaattaCTTATAGTTTTCAATCTCT‑TTTTTTTAACTTGTCTTTCGGTAGTGGCTTCGTTAGTATGATTTTATTTTGTTCCATTTTTACCGGATACCTTTTGTCATCATCCTTTTGTCTGCCGTTACTGTCATCTGTCAtt                      <  2:80426/135‑1 (MQ=255)                                                                                                                       aaGAACTTAAAGTTTTCAATCTCT‑TTTTTTTAACTTGTCATTCGGTAGTGGCTTCGTTGGTATGATTTTATTATGTTCCATTTTTACCGGATACCTTTTTTCATCATCCTTTGGTCTGCCGTTACTGTCATCTGTCATTTc                    <  1:305831/141‑1 (MQ=255)                                                                                                                       aaGAACTTAAAGTTTTCAATCTC‑‑TTTTTTTAACTTGTCATTCGGTAGTGGCTTCGTTGGTATGATTTTATTATGTTCCATTTTTACCGGATACCTTTTTTCATCATCCTTTGGTCTGCCGTTACTGTCATCTGTCATTTCa                   >  1:159279/1‑141 (MQ=255)                                                                                                                        aGAACTTAAAGTTTTCAATCTCT‑TTTTTTTTACTTTTTTTTCGGTAGTGGCTTCGTTCGTATGATTTTATTTTGTTCCATTTTTACCGGATACTTTTTTTTATCATCGTTTGTTCTGCCTTTACTGTCATCTTTTATTTCa                   >  2:678859/1‑141 (MQ=255)                                                                                                                          aaCTTAAAGTTTTCAATCTCT‑TTTTTTTAACTTGTCATTCGGTAGTGGCTTCGTTGGTATGATTTTATTATGTTCCATTTTTACCGGATACCTTTTTTCATCATCCTTTGGTCTGCCGTTACTGTCATCTGTCATTTCACt                 <  1:54197/141‑1 (MQ=255)                                                                                                                               aaaGTTTTCAATCTCT‑TTTTTTTAACTTGTCATTCGGTAGTGGCTTCGTTGGTATGATTTTATTATGTTCCATTTTTACCGGATACCTTTTTTCATCATCCTTTGGTCTGCCGTTACTGTCATCTGTCATTTCACTTATaa            >  1:244867/1‑141 (MQ=255)                                                                                                                               aaaGTTTTCAATCTCT‑TTTTTTTAACTTGTCATTCGGTAGTGGCTTCGTTGGTATGATTTTATTATGTTCCATTTTTACCGGATACCTTTTTTCATCATCCTTTGGTCTGCCGTTACTGTCATCTGTCATTTCACTTATaa            >  1:217953/1‑141 (MQ=255)                                                                                                                               aaaGTTTTCAATCTCT‑TTTTTTTAACTTGTCATTCGGTAGTGGCTTCGTTGGTATGATTTTATTATGTTCCATTTTTACCGGATACCTTTTTTCATCATCCTTTGGTCTGCCGTTACTGTCATCTGTCATTTCACTTATaa            <  2:662239/141‑1 (MQ=255)                                                                                                                               aaaGTTTTCAATCTC‑‑TTTTTTTAACTTGTCATTCGGTAGTGGCTTCGTTGGTATGATTTTATTATGTTCCATTTTTACCGGATACCTTTTTTCATCATCCTTTGGTCTGCCGTTACTGTCATCTGTCATTTCACTTATaaa           <  2:305529/141‑1 (MQ=255)                                                                                                                                     ttCAATCTCT‑TTTTTTTAACTTGTCATTCGGTAGTGGCTTCGTTGGTATGATTTTATTATGTTCCATTTTTACCGGATACCTTTTTTCATCATCCTTTGGTCTGCCGTTACTGTCATCTGTCATTTCACTTATAAaataat      >  2:684444/1‑141 (MQ=255)                                                                                                                                        aaTCTCT‑TTTTTTTAACTTGTCATTCGGTAGTGGCTTCGTTGGTATGATTTTATTATGTTCCATTTTTACCGGATACCTTTTTTCATCATCCTTTGGTCTGCCGTTACTGTCATCTGTCATTTCACTTATAAAATAATAcc   <  2:721871/141‑1 (MQ=255)                                                                                                                                        aaTCTCT‑TTTTTTTAACTTGTCATTCGGTAGTGGCTTCGTTGGTATGATTTTATTATGTTCCATTTTTACCGGATACCTTTTTTCATCATCCTTTGGTCTGCCGTTACTGTCATCTGTCATTTCACTTATAAAATAATAcc   <  2:248090/141‑1 (MQ=255)                                                                                                                                        aaTCTC‑‑TTTTTTTAACTTGTCATTCGGTAGTGGCTTCGTTGGTATGATTTTATTATGTTCCATTTTTACCGGATACCTTTTTTCATCATCCTTTGGTCTGCCGTTACTGTCATCTGTCATTTCACTTATAAAATAATAccc  >  1:939527/1‑141 (MQ=255)                                                                                                                                              |                                                                                                                                         GACATTATAATCATCATTATTCAATTGATATTTTGCCGAATAACTTGGTACATTAGGATTATATGAAATATCACCATCTTTATAATCATTAATATCTTTAAAGTTGCCATATTGTACAAAGAACTTAAAGTTTTCAATCTC‑‑TTTTTTTAACTTGTCATTCGGTAGTGGCTTCGTTGGTATGATTTTATTATGTTCCATTTTTACCGGATACCTTTTTTCATCATCCTTTGGTCTGCCGTTACTGTCATCTGTCATTTCACTTATAAAATAATACCC  >  CP000730/2609932‑2610207

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑