BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,837,203	G→T	7.7%	Q332K (CAA→AAA)	USA300HOU_1714 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,837,203	0	G	T	7.7%	332.1 / 2.9	112	Q332K (CAA→AAA)	USA300HOU_1714	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (44/56); new base T (9/2); total (54/58)
Fisher's exact test for biased strand distribution p-value = 2.44e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

Only 100 of 114 total aligned reads displayed.

AAATTACAAATTATCTAGTACACCTCTAAGTTCACCTAAATGATTAATAACATAGTCGGCATGATGCGCTTCTAACTCACCTGCAGCATCCTTACCTTTTAAACCTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TGAGCACTTAATAAGTCAGCTAACGAATCGCCTACTATAAATACGTCATCTTTATTTACAATGTTATCTTGCTTATTGATATAAGATTCATATTTATCGCGATTATTACCATATAAAGCTGCGATATAACTAAAAGGAT  >  CP000730/1837063‑1837340

aaattacaaattaTCTAGTACACCTCTAAGTTCACCTAAATGATTAATAACATAGTCGGCATGATGCGCTTCTAACTCACCTGCAGCATCCTTACCTTTTAAACCTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑Tg                                                                                                                                           >  1:221210/1‑141 (MQ=255)

aaattacaaattaTCTAGTACACCTCTAAGTTCACCTAAATGATTAATAACATAGTCGGCATGATGCGCTTCTAACTCACCTGCAGCATCCTTACCTTTTAAACCTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑Tg                                                                                                                                           >  2:362876/1‑141 (MQ=255)

   tcacaaattaTCTAGTACACCTCTAAGTTCACCTAAATGATTAATAACATAGTCGGCATGATGCGCTTCTAACTCACCTGCAGCAGCCTTACCTTTTAAACCTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TGAGc                                                                                                                                        <  2:454768/139‑1 (MQ=255)

   atacaaatcaTCTTGTACACCTCTAAGTTCACCTAAATGATTAATAACATAGTCGGCATGATGCGCTTCTAACTCACCTGCAGCATCCTTACCTTTTAAACCTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TGAGc                                                                                                                                        <  2:47023/140‑1 (MQ=255)

     acaaattaTCTAGTACACCTCTAAGTTCACCTAAATGATTAATAACATAGTCGGCATGATGCGCTTCTAACTCACCTGCAGCATCCTTACCTTTTAAACCTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TGAGCAc                                                                                                                                      <  1:486599/141‑1 (MQ=255)

      caaattaTCTAGTACCCCTCTAAGTTCACCTAAATGATTAATAACATAGTCGGCATGATGCGCTTCTAACTCACCTGCAGCATCCTTACCTTTTAAACCTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TGAGCACt                                                                                                                                     <  2:690934/141‑1 (MQ=255)

       aaattaTCTAGTACACCTCTAAGTTCACCTAAATGATTAATAACATAGTCGGCATGATGCGCTTCTAACTCACCTGCAGCATCCTTACCTTTTAAACCTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TGAGCACtt                                                                                                                                    <  1:5152/141‑1 (MQ=255)

         attaTCTAGTACACCTCTAAGTTCACCTAAATGATTAATAACATAGTCGGCATGATGCGCTTCTAACTCACCTGCAGCATCCTTACCTTTTAAACCTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TGAGCACTtaa                                                                                                                                  <  1:487907/141‑1 (MQ=255)

          ttaTCTAGTACACCTCTAAGTTCACCTAAATGATTAATAACATAGTCGGCATGATGCGCTTCTAACTCACCTGCAGCACCCTTAGCTTTTAAACCTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TGAGCACTtaat                                                                                                                                 <  1:103750/141‑1 (MQ=255)

                aGTACACCTCTAAGTTCACCTAAATGATTAATAACATAGTCGGCATGATGCGCTTCTAACTCACCTGCAGCATCCTTACCTTTTAAACCTGTTAATGTTCCAATAAACGTTCCGCCTATTTTT‑TGAGCACTTAATAAGTCa                                                                                                                           >  1:695083/1‑141 (MQ=255)

                aGTACACCTCTAAGTTCACCTAAATGAATAATAACATAGTCGGCATGATGCGCTTCTAACTCACCTGCAGCATCCTTACCTTTTAAACCTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TGAGCACTTAATAAGTCa                                                                                                                           >  1:1067258/1‑141 (MQ=255)

                    cacCTCTAAGTTCACCTAAATGATTAATAACATAGTCGGCATGATGCGCTTCTAACTCACCTGCAGCATCCTTACCTTTTAAACCTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TGAGCACTTAATAAGTCAGCTa                                                                                                                       <  2:264943/141‑1 (MQ=255)

                     acCTCTAAGTTCACCTAAATGATTAATAACATAGTCGGCATGATGCGCTTCTAACTCACCTGCAGCATCCTTACCTTTTAAACCTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TGAGCACTTAATAAGTCAGCTaa                                                                                                                      <  1:1095796/141‑1 (MQ=255)

                        tctAAGTTCACCTAAATGATTAATAACATAGTCGGCATGATGCGCTTCTAACTCACCTGCAGCATCCTTACCTTTTAAACCTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TGAGCACTTAATAAGTCAGCTAACGa                                                                                                                   >  2:963736/1‑141 (MQ=255)

                        tctAAGTTCACCTAAATGATTAATAACATAGTCGGCATGATGCGCTTCTAACTCACCTGCAGCATCCTTACCTTTTAAACCTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TGAGCACTTAATAAGTCAGCTAACGa                                                                                                                   >  1:1163426/1‑141 (MQ=255)

                         ctAAGTTCACCTAAATGATTAATAACATAGTCGGCATGATGCGCTTCTAACTCACCTGCAGCATCCTTACCTTTTAAACCTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TGAGCACTTAATAAGTCAGCTAACGaa                                                                                                                  >  1:126745/1‑141 (MQ=255)

                          tAAGTTCACCTAAATGATTAATAACATAGTCGGCATGATGCGCTTCTAACTCACCTGCAGCATCCTTACCTTTTAAACCTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TGAGCACTTAATAATTCAGCTAACGAAt                                                                                                                 >  2:68016/1‑141 (MQ=255)

                            aGTTCACCTTAATGATTAATAACATTGTCGGCATGATGCGCTTCTAACTCACCAGCAGCATCCTTACCTTTTAAACCTGTTAATGTTCCAAGAAACGTTGCACCTATTTTT‑TGAGCACTTAATAAGTCAGCTAACGAATCg                                                                                                               <  2:655407/141‑1 (MQ=255)

                            aGTTCACCTACATGGTTGATAGCAGTGTCGGCAGTATGCGCTTCTAACTCACCTGCAGCATCCTTACCTTTTATACCTGTTAATGTTCCAATAATCGTTGCACCTATTTTT‑TGAGCTCTTAATAAGTCAGCTAACGAATCg                                                                                                               <  2:655561/141‑1 (MQ=255)

                                 aCCTAAATGATTAATAACATAGTCGGCATGATGCGCTTCTAACTCACCTGCAGCATCCTTACCTTTTAAACCTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TTTTCTCTTTTTAAGTCAGCTAACGAATCGCctac                                                                                                          >  2:177168/1‑141 (MQ=255)

                                  ccTAAATGATTAATAACATAGTCGGCATGATGCGCTTCTAACTCACCTGCAGCATCCTTACCTTTTAAACCTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TGAGCACTTAATAAGTCAGCTAACGAATCGCctact                                                                                                         <  2:1086638/141‑1 (MQ=255)

                                  ccTAAATGATTAATAACATAGTCGGCATGATGCGCTTCTAACTCACCTGCAGCATCCTTACCTTTTAAACCTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TGAGCACTTAATAAGTCAGCTAACGAATCGCctact                                                                                                         <  2:174639/141‑1 (MQ=255)

                                    tAAATTATTAATAACATAGTCGGCATGATGCGCTTCTAACTCACCTGCAGCATCCTTACCTTTTAAACCTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TGAGCACTTAATAAGTCAGCTAACGAATCGCCTACtat                                                                                                       <  2:1097522/141‑1 (MQ=255)

                                     aaaTGATTAATAACATAGCCGGCATGATGCGCTTCTAACTCACCTGCAGCATCCTTATCTTTTAAACCTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TGAGCACTTAATAAGTCAGCTAACGAATCGCCTACtata                                                                                                      <  2:832154/141‑1 (MQ=255)

                                      aaTGATTAATAACATAGTCGGCATGATGCGCTTCTAACTCACCTGCAGCATCCTTACCTTTTAAACCTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TGAGCACTTAATAAGTCAGCTAACGACTCGCCTACTATaa                                                                                                     <  2:817182/141‑1 (MQ=255)

                                       aTGATTAATAACATAGTCGGCATGATGCGCTTCTAACTCACCTGCAGCATCCTTACCTTTTAAACCTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TGAGCACTTAATAAGTCAGCTAACGAATCGCCTACTATaaa                                                                                                    <  1:1077839/141‑1 (MQ=255)

                                         gATTAATAACATAGTCGGCATGATGCGCTTCTAACTCACCTGCAGCATCCTTACCTTTTAAACCTGTTAATGTTCCGATAAACGTTGCACCTATTTTT‑TGAGCACTTAATAAGTCAGCTCACGAATCGCCTACTATAAATa                                                                                                  >  2:864595/1‑141 (MQ=255)

                                         gATTAATAACATAGTCGGCATGATGCGCTTCTAACTCACCTGCAGCATCCTTACCTTTTAAACCTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TGAGCACTTAATAATTCAGCTTACGACTCGCCTCTTTTAAATa                                                                                                  >  2:1118134/1‑141 (MQ=255)

                                         gATTAATAACATAGTCGGCATGATGCGCTTCTAACTCACCTGCAGCATCCTTACCTTTTAAACCTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TGAGCACTTAATAAGTCAGCTAACGAATCGCCTACTATAAATa                                                                                                  >  2:301269/1‑141 (MQ=255)

                                          aTTAATAACATAGTCGGCATGATGCGCTTCTAACTCACCTGCAGCATCCTTACCTTTTAAACCTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TGAGCACTTAATAAGTCAGCTAACGAATCGCCTACTATAAATAc                                                                                                 <  1:459945/141‑1 (MQ=255)

                                           ttAATAACATAGTCGGCATGATGCGCTTCTAACTCACCTGCAGCATCCTTACCTTTTAAACCTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TGAGCACTTAATAAGTCAGCTAACGAATCGCCTACTATAAATACg                                                                                                <  1:1057597/141‑1 (MQ=255)

                                              ataaCATAGTCGGCATGATGCGCTTCTAACTCACCTGCAGCATCCTTACCTTTTAAACCTGTTAATGTTCCAATAAACGTTGCACCTCTTTTT‑TGAGCCCTTAATAAGTCAGCTACCGGATCGCCTACGATAAATACGTCa                                                                                             >  1:1049518/1‑141 (MQ=255)

                                                aaCATAGTCCGCATGATGCGCTTCTAACTCACCTGCAGCATCCTTACCTTTTAAACCTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TGAGCACTTAATAAGTCAGCTAACGAATCGCCTACTATAAATACGTCATc                                                                                           <  1:862732/141‑1 (MQ=255)

                                                  cATAGTCGGCATGATGCGCTTCTAACTCACCTGCAGCATCCTTACCTTTTAACCCTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TGAGCACTTAATAAGTCAGCTAACGAATCGCCTACTATAAATACGTCATCtt                                                                                         <  1:1111933/141‑1 (MQ=255)

                                                  cATAGTCGGCATGATGCGCTTCTAACTCACCTGCAGCATCCTTACCTTTTAAACCTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TGAGCACTTAATAAGTCAGCTAACGAATCGCCTACTATAAATACGTCATCtt                                                                                         <  2:391639/141‑1 (MQ=255)

                                                    tatttggCATTATGCGCTTCTAACTCACCTGCAGCATCCTTACCTTTTAAACCTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TTAGCACTTAATAAGTCATCTAACGAATCGCCTACTATAAATACGTCATCttta                                                                                       <  2:584382/136‑1 (MQ=255)

                                                    tAGTCGGCATGATGCGCTTCTAACTCACCTGCAGCATCCTTACCTTTTAAACCTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TGAGCACTTAATAAGTCAGCTAACGAATCGCCTACTATAAATACGTCATCttta                                                                                       >  1:145683/1‑141 (MQ=255)

                                                    tAGTCGGCATGATGCGCTTCTAACTCACCTGCAGCATCCTTACCTTTTAAACCTGTTAATGTTACCATAAACGTTGCACCTATTTTTCTTTGTTTGTAA‑AATACAGCTAACGAATCACCGACTGTAAATACGTCATCttta                                                                                       >  1:864304/1‑141 (MQ=255)

                                                       tCGGCATGATGCGCTTCTAACTCACCTGCAGCATCCTTACCTTTTAAACCTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TGAGCACTTAATAAGTCAGCTAACGAATCGCCTACTATAAATACGTCATCtttattt                                                                                    <  1:766150/141‑1 (MQ=255)

                                                          gCATGATGCGCTTCTAACTCACCTGCAGCATCCTTACCTTTTAAACCTGTTAATGTTCCAATAAACGTTGCACCTCTTTTT‑TGAGCACTTAATAAGTCAGCTAACGAATCGCCTACTATCAATCCGTCCTCTTTATTTACa                                                                                 >  1:290348/1‑141 (MQ=255)

                                                          gCATGATGCGCTTCTAACTCACCTGCAGCATCCTTACCTTTTAAACCTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TGAGCACTTAATAAGTCAGCTAACGAATCGCCTACTATAAATACGTCATCTTTATTTACa                                                                                 <  1:211341/141‑1 (MQ=255)

                                                           cATGATGCGCTTCTAACTCACCTGCAGCATCCTTACCTTTTAAACCTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TGAGCACTTAATAAGTCAGCTAACGAATCGCCTACTTTACATACGTCATCTTTATTTTCaa                                                                                >  2:516671/1‑141 (MQ=255)

                                                           cATGATGCGCTTCTAACTCACCTGCAGCATCCTTACCTTTTAAACCCGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TGAGCACTTAATAAGTCAGCTAACGAATCGCCTACTATAAATACGTCATCCTTATTTACaa                                                                                <  2:810246/141‑1 (MQ=255)

                                                            atgatgCGCTTCTAACTCAGCTGCAGCATCCTTACCTTTTAAACCTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TGAGCACTTAATAAGTCAGCTAACGAATCGCCTACTACAAATACGTCATCTTTATTTACAAt                                                                               <  1:762312/141‑1 (MQ=255)

                                                             tgatgCGCTTCTAACTCACCTGCAGCATCCTTACCTTTTAAACCTGTCAATGTTCCAATAAACGTTGCACCTATTTTT‑TGAGCACTTAATAAGTCAGCTAACGAATCGCCTACTATAAATACGTCATCTTTATTTACAATg                                                                              <  1:221611/141‑1 (MQ=255)

                                                             tgatcCGCTTCCAACTCACCCGCAGCATACTTACCTTTTAAACCTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TGAGCACTTAATAAGTCAGCTAACGAATCGCCTACTATAAATACGTCATCTTTATTTACAATg                                                                              <  1:598931/141‑1 (MQ=255)

                                                               atgCGCTTCTAACTCACCTGCAGCATCCTTACCTTTTAAACCTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TGAGCACTTAATAAGTCAGCTAACGAATCGCCTACTATAAATACGTCATCTTTATTTACAATGtt                                                                            >  2:72861/1‑141 (MQ=255)

                                                                tgCGCTTCTAACTCACCTGCAGCATCCTTACCTTTTAAACCTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TGAGCACTTAATAAGTCAGCTAACGAATCGCCTACTATAAATACGTCATCTTTATTTACAATGtca                                                                           >  2:958463/1‑139 (MQ=255)

                                                                   gcTTCTAACTCACCTGCAGCATCCTTACCTTTTAAACCTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TGAGCACTTAATAAGTCAGCTAACGAATCGCCTACTATAAATACGTCATCTTTATTTACAATGTTATCt                                                                        <  2:101262/141‑1 (MQ=255)

                                                                    tttCTAACTCACCTGCAGCATCCTTACCTTTTAAACCTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TGAGCACTTAATAAGTCAGCTAACGAATCGCCTACTATAAATACGTCATCTTTATTTACAATGTTATCtt                                                                       <  1:261197/140‑1 (MQ=255)

                                                                    cctttAACTCACCTGCAGCATCCTTACCTTTTAAACCTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TGAGCACTTAATAAGTCAGCTAACGAATCGCCTACTATAAATACGTCATCTTTATTTACAATGTTATCtt                                                                       <  2:449084/137‑1 (MQ=255)

                                                                    cTTCTAACTCACCTGCAGCATCCTTACCTTTTAAACCTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TTTGCACTTAATAAGTGAGCTAAGCAATCGACTACTTTAAATACGTCATGTTTATTTACAATGTTATCtt                                                                       >  2:105334/1‑141 (MQ=255)

                                                                    cTTCCAACTCACCGGCAGCATCCCTAACTTTTAAACCTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TGAGCACTGAATAAGTCAGCTAACGAATCGCCTACTATCAATACGTCATCTTTATTTACAATGTTATCtt                                                                       <  2:306362/141‑1 (MQ=255)

                                                                     ttCTAACTCACCTGCAGCATCCTTACCTTTTAAACCTGTTAATGTTCCAATAAACGTTGCCCCTATTTTT‑TGAGCACTTAATAAGTCAGCTAACGAATCGCCTACTATAAATACGTCATCTTTATTTACAATGTTATCTTg                                                                      >  2:494983/1‑141 (MQ=255)

                                                                       cTAACTCACCTGCAGCATCCTTACCTTTTAAACCTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TGAGCACTTAATAAGTCAGCTAACGAATCGCCTACTATAAATACGTCATCTTTATTTACAATGTTATCTTGCt                                                                    <  1:652159/141‑1 (MQ=255)

                                                                       cTAACTCACCTGCAGCATCCTTACCTTTTAAACCTGCTAATGTTCCAATAAACGTTGCACCTATTTTT‑TGAGCACTTAATAAGTCAGCTAACGCATCGCCTACTATAAATACGTCATCTTTATTTACAATGCTATCTTGCt                                                                    <  2:769219/141‑1 (MQ=255)

                                                                         aaCTCACCTGCAGCATCCTTACCTTTTAAACCTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TGAGCACTTAATAAGTCAGCTAACGAATCGCCTACTATAAATACGTCATCTTTATTTACAATGTTATCTTGCttt                                                                  >  2:489539/1‑140 (MQ=255)

                                                                           cTCACCTGCAGCATCCTTACCTTTTAAACCTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TGAGCACTTAATAAGTCAGCTAACGAATCGCCTACTATAAATACGTCATCTTTATTTACAATGTTATCTTGCTTAtt                                                                <  1:587713/141‑1 (MQ=255)

                                                                           cTCACCTGCAGCATCCTTACCTTTTAAACCTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TGAGCACTTAATAAGTCAGCTAACGAATCGCCTACTATAAATACGTCATCTTTATTTACAATGTTATCTTGCTTAtt                                                                <  1:435170/141‑1 (MQ=255)

                                                                             cACCTGCAGCATCCTTACCTTTTAAACCTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TGAGCACTTAATAAGTCAGCTAACGAATCGCCTACTATAAATACGTCATCTTTATTTACAATGTTATCTTGCTTATTGa                                                              >  1:391727/1‑141 (MQ=255)

                                                                               ccTGCAGCATCCTTACCTTTTAATCCTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TGAGCACTTAATAAGTCAGCTAACGAATCGCCTACTATAAATACGTCATCTTTATTTACAATGTTATCTTGCTTATTGata                                                            <  2:277361/141‑1 (MQ=255)

                                                                               ccTGCAGCATCCTTACCTTTTAAACCTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TGAGCACTTAATAAGTCAGCTAACGAATCGCCTACTATAAATACGTCATCTTTATTTACAATGTTATCTTGCTTATTGata                                                            <  1:1174449/141‑1 (MQ=255)

                                                                                   cagcaTCCTTACCTTTTAAACCTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TGAGCACTTAATAAGTCAGCTAACGAATCGCCTACTATAAATACGTCATCTTTATTTACAATGTTATCTTGCTTATTGATATAAg                                                        >  1:141502/1‑141 (MQ=255)

                                                                                    agcaTCCTTACCTTTTAAACCTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TGAGCACTTAATAAGTCAGCTAACGAATCGCCTACTATAAATACGTCATCTTTATTTACAATGTTATCTTGCTTATTGATATAAGa                                                       >  1:4568/1‑141 (MQ=255)

                                                                                     gcaTCCTTACCTTTTAAACCTGTTAATGTTCCAATAAACGTTGCACGTATTTTT‑TGAGCACTTCATAAGTCAGCTAACGAATCGCCTACTATAAATACGGCATCTTTATTTACAATGTTATCTTGCTGATTGATATAAGAt                                                      >  1:68787/1‑141 (MQ=255)

                                                                                     gcaTCCTTACCTTTTAAACCTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TTTTCATTTTATAAGTCAGCTAACGAATCGCCTACTATAAATAAGTCATCTTTATTTACAATGTTATCTTGCTTATTGATACAAGAt                                                      >  2:1100692/1‑141 (MQ=255)

                                                                                     gcaTCCTTACCTTTTAAACCTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TGAGCACTTAATAAGTCAGCTAACGAATCGCCTACTATAAATACGTCATCTTTATTTACAATGTTATCTTGCTTATTGATATAAGAt                                                      >  1:69484/1‑141 (MQ=255)

                                                                                         ccTTACCTTTTAAACCTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TGAGCACTTAATAAGTGAGCTAACGAATCGCCTACGATAAATACGTCATCTTTATTTACAATGTTATCTTGCTTATTGATATAAGGTTCat                                                  >  1:46875/1‑141 (MQ=255)

                                                                                         ccTTACCTTTTAAACCTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TGAGCACTTAATAAGTCAGCTAACGAATCGCCTACTATAAATACGTCATCTTTATTTACAATGTTATCTTGCTTATTGATATAAGATTCat                                                  >  2:579682/1‑141 (MQ=255)

                                                                                            tACCTTTTAAACCTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TGAGCACTTAATAAGTCAGCTAACGAATCGCCTACTATAAATACGTCATCTTTATTTACAATGTTATCTTGCTTATTGATATAAGATTCATAtt                                               <  1:740268/141‑1 (MQ=255)

                                                                                             aCCTTTTAAACCTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TTTTCACTTTATAAGTCAGCTAACGAATCGCCTACTGTAAATAAGTCATCTTTATTTACAATGTTATCTTCCTTATTCATATAACATTCATtttt                                              >  2:788543/1‑141 (MQ=255)

                                                                                                ttttAAACCTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TGAGCACTTAATAAGTCAGCTAACGAATCGCCTACTATAAATACGTCATCTTTATTTACAATGTTATCTTGCTTATTGATATAAGATTCATATTTATc                                           <  2:502161/141‑1 (MQ=255)

                                                                                                 tttAACCCTGTTAATGTTCCAATAAACGTTGCCCCTATTTTT‑TGAGCACTTAATAAGTCAGCTAACGAATCGCCTACTATAAATACGTCATCTTTATTTACAATGTTATCTTGCTTATTGATATAAGATTCATATTTATcg                                          <  1:177168/141‑1 (MQ=255)

                                                                                                  ttAATCCTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TGAGCACTTAATAAGTCAGCTAACGAATCGCCTACTATAAATACGTCATCTTTATTTACAATGTTATCTTGCTTATTGATATAAGATTCATATTTATcgc                                         <  2:221210/141‑1 (MQ=255)

                                                                                                   tAAACCTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TGAGCACTTAATAAGTCAGCTAACTCATCGCCTACTATAAATACGTCATCTTTATTTACAATGTTAGCTTGCTTATTGATATAACATTCATATTTATcacg                                        >  2:127288/1‑141 (MQ=255)

                                                                                                     atccTGTTATTGTTTTTATAAACGTTGCACCTATTTTT‑TGATCACTTAATAAGTCAGCTTACTAATCGCCTACTATAAATACGTCATCTTTATTTACAATGTTATCTTGCTTATTGATATAAGATTCATATTTATCGCGat                                      <  2:1129612/139‑1 (MQ=255)

                                                                                                       ccTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TGAGCACTTAATAAGTCAGCTAACGAATCGCCTACTATAAATACGTCATCTTTATTTACAATGTTATCTTGCTTATTGATATAAGATTCATATTTATCGCGatta                                    <  1:116873/141‑1 (MQ=255)

                                                                                                        cTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TTTTTTTTTTTTAAGTCAGCTAACGAATCGCCTACTATAACTACGTAATCTTTATTTACAATGTTATCTTCCTTTTTCATTTTACATTCATTTTTTTCGCGattct                                   >  2:187504/1‑139 (MQ=255)

                                                                                                        cTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TTTTTTTTTTTTAAGTCAGATAACGACTCGCCTACTATAAATACGTCATCTTTATTTACAATGTTATCTTTCTTATTTATATTATATACATATTTATCCTGatttt                                   >  2:587111/1‑139 (MQ=255)

                                                                                                        cTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TTTGTTTTTTTTAAGTCAGCTATCGAAGCGCCTATTATAAATATTTCATGTTTTTTTACAAGTTTGTCTTGCTTATTGATTTTAGATTCTTTTTTTTctctgttgt                                   >  2:2267/1‑133 (MQ=255)

                                                                                                        cTGTAAATGTTCCAATAAACGTTGCACCTATTTTT‑TGAGCACTTAATAAGTCAGCTAACGAATCGCCTACTATAAATACGTCATCTTTATTTACAATGTTATCTTGCGTATTGATATAAGATTCATATTTATCGCGattat                                   >  1:814312/1‑141 (MQ=255)

                                                                                                         tGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TGAGCACTTAATAAGTCAGCTAACGAATCGCCTACTATAAATACGTCATCTTTATTTACAATGTTATCTTGCTTATTGATATAAGATTCATATTTATCGCGattatt                                  >  1:1193631/1‑141 (MQ=255)

                                                                                                          gTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TTTTTTTTTTTTAAGTCAGATAACGCATCGCCTACTATAAATACGTCATCTTTATTTACACTGTTTTCTTGCTTATTTATTTAAGATTCATATTTATCTCGaatattt                                 >  2:40667/1‑140 (MQ=255)

                                                                                                             aaTGTTCCAATAAACGTTGCACCTATTTTG‑TGAGCACTTAATAAGTCAGCTAACGAATCGCCTACTATAAATACGTCATCTTTATTTACAATGTTATCTTGCTTATTGATATAAGATTCATATTTATCGCGATTATTACCa                              >  2:578532/1‑141 (MQ=255)

                                                                                                              aTGTTCCAATAAACGTTGCACCTATTTTT‑TGAGCACTTAATAAGTCAGCTAACGAATCGCCTACTATAAATACGTCATCTTTATTTACAATGTTATCTTGCTTATTGATATAAGATTCATATTTATCGCGATTATTACCat                             >  1:521158/1‑141 (MQ=255)

                                                                                                                gTTCCAATAAACGTTGCACCTATTTTT‑TGAGCACTTAATAAGTCAGCTAACGAATCGCCTACTATAAATACGTCATCTTTATTTACAATGTTATCTTGCTTATTGATATAAGATTCATATTTATCGCGATTATTACCatat                           <  1:362876/141‑1 (MQ=255)

                                                                                                                 ttCCAATAAACGTTGCACCTATTTTT‑TGAGCACTTAATAAGTCAGCTAACGAATCGCCTACTATAAATACGTCATCTTTATTTACAATGTTATCTTGCTTATTGATATAAGATTCATATTTATCGCGATTATTACCAtata                          <  2:145683/141‑1 (MQ=255)

                                                                                                                 ttCCAATAAACGTTGCACCTATTTTT‑TGAGCACTTAATAAGTCAGCTAACGAATCGCCTACTATAAATACGTCATCTTTATTTACAATGTTATCTTGCTTATTGATATAAGATTCATATTTATCGCGATTATTACCAtata                          <  1:963736/141‑1 (MQ=255)

                                                                                                                 ttCCAATAAACGTTGCACCTATTTTT‑TGAGCACTTAATAAGTCAGCTAACGAATCGCCTACTATAAATACGTCATCTTTATTTACAATGTTATCTTGCTTATTGATATAAGATTCATATTTATCGCGATTATTACCAtata                          <  2:1163426/141‑1 (MQ=255)

                                                                                                                      aTAAACGTTGCACCTATTTTT‑TGAGCACTTAATAAGTCAGCTAACGAATCGCCTACTATAAATACGTCATCTTTATTTACAATGTTATCTTGCTTATTGATATAAGATTCATATTTATCGCGATTATTACCATATAAAGCt                     >  1:339078/1‑141 (MQ=255)

                                                                                                                       tAAACGTTGCACCTATTTTT‑TGAGCACTTAATAAGTCAGCTAACGAATCTCCTACTATAAATACATCTTCTTTATTTACAATGTTATCTTGCTTTTTGATATAAGATTCATATTTATCGCGATTATTACCATTTAAAGCTg                    >  2:696/1‑141 (MQ=255)

                                                                                                                          aCGTTGCACCTATTTTT‑TGAGCACTTAATAAGTCAGCTAACGAATCGCCTACTATAAATACGTCATCTTTATTTACAATGTTATCTTGCTTATTGATATAAGATTCATATTTATCGCGATTATTACCATATAAAGCTGCGa                 >  2:946539/1‑141 (MQ=255)

                                                                                                                            gTTGCACCTATTTTT‑TGAGCACTTAATAAGTCAGCTAACGAATCGCCTACTATAAATACGTCATCTTTATTTACAATGTTATCTTGCTTATTGATATAAGATTCATATTTATCGCGCATATTACCATATAAAGCTGCGata               <  1:579682/141‑1 (MQ=255)

                                                                                                                            gTTGCACCTATTTTT‑TGAGCACTTAATAAGTCAGCTAACGAATCGCCTACTATAAATACGTCATCTTTATTTACAATGTTATCTTGCTTATTGATATAAGATTCATATTTATCGCGATTATTACCATATAAAGCTGCGata               <  1:646148/141‑1 (MQ=255)

                                                                                                                              tGCACCTATTTTT‑TGAGCACTTAATAAGTCAGCTAACGAATCGCCTACTATAAATACGTCATCTTTATTTACAATGTTATCTTGCTTATTGATATAAGATTCATATTTATCGCGATTATTACCATATAAAGCTGCgtcatt             >  2:1076558/1‑136 (MQ=255)

                                                                                                                              tGCACCTATTTTT‑TGAGCACTTAATAAGTCAGCTAACGAATCGCCTACTATAAATACGTCATCTTTATTTACAATGTTATCTTGCTTATTGATATAAGATTCATATTTATCGCGATTATTACCATATAAAGCTGCGAtata             >  2:626609/1‑141 (MQ=255)

                                                                                                                                  ccTATTTTT‑TTAGCACTTAATAAGTCAGCTAACGAATCGCCTACTATAAATACGTCATCTTTATTTACAATGTTATCTTGCTTATTGATATAAGATTCATATTTATCGCGATTATTACCATATAAAGCTGCGATATAACTa         <  2:927178/141‑1 (MQ=255)

                                                                                                                                     aTTTTT‑TGAGCACTTAATAAGTCAGCTAACGAATCGCCTACTATAAATACGTCATCTTTATTTACAATGTTATCTTGCTTATTGATATAAGATTCATATTTATCGCGATTATTACCATATAAAGCTGCGATATAACTaaaa      >  1:712568/1‑141 (MQ=255)

                                                                                                                                         tt‑tGAGCACTTAATAAGTCAGCTAACGAATCGCCTACTATAAATACGTCATCTTTATTTACAATGTTATCTTGCTTATTGATATAAGATTCATATTTATCGCGATTATTACCATATAAAGCTGCGATATAACTAAAAGGAt  <  1:1021764/141‑1 (MQ=255)

                                                                                                                                         tt‑tGAGCACTTAAGAAGTCAGCTAACGAAGCGCCTAATATAAATGCGTCAGCTTTATTTACAATGTTATCTTGCTTATTGATATAAGATTCATATTTATCGCGATTATTACCATATAAAGCTGCGATATAACTAAAAGGAt  <  1:30006/141‑1 (MQ=255)

AAATTACAAATTATCTAGTACACCTCTAAGTTCACCTAAATGATTAATAACATAGTCGGCATGATGCGCTTCTAACTCACCTGCAGCATCCTTACCTTTTAAACCTGTTAATGTTCCAATAAACGTTGCACCTATTTTT‑TGAGCACTTAATAAGTCAGCTAACGAATCGCCTACTATAAATACGTCATCTTTATTTACAATGTTATCTTGCTTATTGATATAAGATTCATATTTATCGCGATTATTACCATATAAAGCTGCGATATAACTAAAAGGAT  >  CP000730/1837063‑1837340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`