Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	2,532,298	A→T	T15T (ACA→ACT)	murN →	FemAB family peptidoglycan biosynthesis protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,532,298	0	A	T	100.0%	84.0 / NA	29	T15T (ACA→ACT)	murN	FemAB family peptidoglycan biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (5/24);  total (5/24)

GTGTATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGA‑TGAGTTCAATGCGTTTACAACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAGCA  >  CP000730/2532162‑2532433                                                                                                                                          |                                                                                                                                        gtttATTTGTAATCAGTTTTTAATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATTGGATAAAATTAACTTTGTAACGTTGACTTCGGA‑TTAGTTCAATTCTTTTActacaa                                                                                                                                     <  2:276101/138‑1 (MQ=255)      tttGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGA‑TGAGTTCAATGCGTTTACTACAAAGCAt                                                                                                                                >  2:456079/1‑141 (MQ=255)         gTAAACAGATTTAGATAAAATAAAAAAATAGCAATATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGA‑TGAGTTCAATGCGTTTACTACAAAGCAtttt                                                                                                                             <  1:1341/141‑1 (MQ=255)          tAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGA‑TGAGTTCAATGCGTTTACTACAAAGCAttttt                                                                                                                            <  2:495331/141‑1 (MQ=255)          tAATCAGTTTGTGAAAAAATAAAAAAAAAGCATTATGAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGA‑TCAGTAGGTGGCGGTTACTACAAAGCAttttt                                                                                                                            <  2:352676/141‑1 (MQ=255)              cagatatttataaaataaaAATATAGCCTTATTAATAAATTCAAAAATTATAGTTCGATACAACCAGCGATGGTATAAAATGAACTTTGTAATGTTGACTTCGAA‑TGAGTTCAATGCTTATACTATAAAGCATTTCTcaaa                                                                                                                        >  2:84399/10‑139 (MQ=255)                    ttaaaaaaataaaaAAATAGCATTATTAATAAATTCAAAAATTATAGTTCGATTCAGAGAGGGCTGGGATAAAATGAACTTTGTAACGTTGACTTCGGA‑TGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTacac                                                                                                                  <  2:340874/136‑1 (MQ=255)                    ttGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGA‑TGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTacac                                                                                                                  <  2:30686/141‑1 (MQ=255)                         aaaataaaaAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGA‑TGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAAt                                                                                                             <  2:242449/141‑1 (MQ=255)                                  aaaTGGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGA‑TGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTc                                                                                                    <  2:225066/141‑1 (MQ=255)                                    aTAGCAATATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTCACTTCGGA‑TGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCAt                                                                                                  <  2:60542/141‑1 (MQ=255)                                             ttaATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGA‑TGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCAt                                                                                         <  2:446863/141‑1 (MQ=255)                                               aataaATTCAAAAATTAGAGTTCAATTCAGAGAGGGATGCGATAAAATCAACTATGTAACGCTGACTTCCTA‑TGACTTCAACGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCCATTCATTACAATCATag                                                                                       <  2:425461/141‑1 (MQ=255)                                                                         gcaggtgagtcttggatAAAATTAACTTGGTAACGGTGCCTTCGGAGTGGGTT‑AAGGCGTTTACTGCACAGCGCTTTTCACCTTACACCCAATCCGCTGTTGATTACCATCAGAGCGTTGGTTTAAAAGGCGATGTGCATc                                                             <  2:249568/128‑1 (MQ=255)                                                                           agagagGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGA‑TGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCaaatg                                                           <  2:456797/141‑5 (MQ=255)                                                                           agagagGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGA‑TGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCtt                                                           <  2:291500/141‑1 (MQ=255)                                                                           agagagGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGA‑TGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCtt                                                           <  2:279990/141‑1 (MQ=255)                                                                                gggatgggatAAAATGAACTTTGTAACGTTGACTTCGGA‑TGAGTTCAATGTGTTTACTAGAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAgg                                                      <  2:297419/141‑1 (MQ=255)                                                                                     gggatAAAATGAACTTTGTAACGTTGACTTCGGA‑TGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTa                                                 >  2:263304/1‑141 (MQ=255)                                                                                               gAACTGTCTAACGGTGACTTCGGA‑TGAGTTCAAGGCGTTTACTAGAAAGCATTTTGCTCCTTACACACAATCTGCGACTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACaa                                       <  2:185872/141‑1 (MQ=255)                                                                                                aaCTTTGTAACGTTGACTTCGGA‑TGGGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGAcgtt                                      <  2:305132/141‑4 (MQ=255)                                                                                                 aCTTTGTAACGTTGACTTCGGA‑TGGGTTCAAGGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATg                                     >  2:239859/1‑141 (MQ=255)                                                                                                    ttGTAACGTTGACTTCGGA‑TGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATTAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGTGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTc                                  <  2:370817/141‑1 (MQ=255)                                                                                                       tAACGTTGACTTCGGA‑TGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTCGATCGGGGGAAAGATGACAATGGTCAAg                               <  2:227830/141‑1 (MQ=255)                                                                                                         aCGTTGACTTCGGA‑TGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTg                             <  2:291374/141‑1 (MQ=255)                                                                                                                 ttCGGA‑TTGCTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTGGGGGTTAAAGATGACAATGGTCAAGTGATTGCAgg                     <  2:82915/141‑1 (MQ=255)                                                                                                                   tggA‑TGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGAt                   <  2:394348/140‑1 (MQ=255)                                                                                                                             tCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGa          <  2:274964/141‑1 (MQ=255)                                                                                                                                   cGTTTACTCCAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAg    <  2:64168/141‑1 (MQ=255)                                                                                                                                     tttACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCAACACAATCAGAGATCTGATTTAAACGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTCCAGGATGCTTATTGACAGAAgca  >  2:478660/1‑141 (MQ=255)                                                                                                                                          |                                                                                                                                        GTGTATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGA‑TGAGTTCAATGCGTTTACAACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAGCA  >  CP000730/2532162‑2532433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑