Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP000730	1,232,053	A→T	D233V (GAT→GTT)	prpC →	possible PP2C protein phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,232,053	0	A	T	100.0%	50.5 / NA	15	D233V (GAT→GTT)	prpC	possible PP2C protein phosphatase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (12/3);  total (12/3)

TGATTATTTATTATTAAATTCAGATGGATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATG‑CAATTGGCATTAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAA  >  CP000730/1231913‑1232193                                                                                                                                              |                                                                                                                                             tGATTATTTATTATTAAATTCAGATGGATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTAAAATAGAAGATCATGGTGATCAATTAATG‑CAATTGGCATTAGATAACCATTCGAAAGTTAACGTTACt                                                                                                                                    >  2:158129/1‑151 (MQ=255)                     cAGATGGATTAACTGATTATGTTAAANACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATG‑CAATTGGCATTAGATAACCATTCGAAAGTTAACGTTACTTTCATACTCGCGGCTATTGa                                                                                                                >  1:305026/1‑151 (MQ=255)                                 cTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATG‑CAATTGGCATTAGATAACCATTCGAAAGTTAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGt                                                                                                    >  1:295607/1‑151 (MQ=255)                                  tGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATG‑CAATTGGCATTAGATAACCATTCGAAAGTTAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTa                                                                                                   >  1:209224/1‑151 (MQ=255)                                         gTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATG‑CAATTGGCATTAGATAACCATTCGAAAGTTAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAgg                                                                                            >  1:256487/1‑151 (MQ=255)                                               gACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATG‑CAATTGGCATTAGATAACCATTCGAAAGTTAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAaat                                                                                      <  1:154254/151‑1 (MQ=255)                                                                                 gTACAATAGAAGATCATGGTGATCAATTAATG‑CAATTGGCATTAGATAACCATTCGAAAGTTAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCtt                                                    >  2:358096/1‑151 (MQ=255)                                                                                                          tctAATGCCAATT‑GCATTAGATAACCATTCGAAAGTTAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGttt                           >  1:186115/3‑151 (MQ=255)                                                                                                           ttAATG‑CAATTGGCATTAGATAACCATTCGAAAGTTAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGTTTa                          <  2:256487/151‑1 (MQ=255)                                                                                                            tAATG‑CAATTGGCATTAGATAACCATTCGAAAGTTAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGTTTAt                         >  2:198310/1‑151 (MQ=255)                                                                                                             aaTG‑CAATTGGCATTAGATAACCATTCGAAAGTTAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGTTTATc                        <  2:26161/151‑1 (MQ=255)                                                                                                              aTG‑CAATTGGCATTAGATAACCATTCGAAAGTTAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGTTTATCt                       >  2:175061/1‑151 (MQ=255)                                                                                                                          aTTAGATAACCATTCGAAAGTTAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGTTTATCTTGCTGAAgata            >  1:124258/1‑151 (MQ=255)                                                                                                                                   ccATTCGAAAGTTAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGTTTATCTTGATGAAGATACGATACTTa   >  1:188221/1‑151 (MQ=255)                                                                                                                                    cATTCGAAAGTTAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTaa  >  1:298552/1‑151 (MQ=255)                                                                                                                                              |                                                                                                                                             TGATTATTTATTATTAAATTCAGATGGATTAACTGATTATGTTAAAGACAATGAAATTAAGCGTTTGTTAGTAAAAGAAGGTACAATAGAAGATCATGGTGATCAATTAATG‑CAATTGGCATTAGATAACCATTCGAAAGATAACGTTACTTTCATACTCGCGGCTATTGAAGGTGATAAAGTATGATAGGTAAAATAATAAATGAACGATATAAAATTGTAGATAAGCTTGGCGGCGGTGGCATGAGTACCGTTTATCTTGCTGAAGATACGATACTTAA  >  CP000730/1231913‑1232193

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑