Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	2,544,276	T→C	8.5%	Y168Y (TAT→TAC)	hlgC →	gamma hemolysin component C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,544,276	0	T	C	8.5%	143.3 / 4.1	59	Y168Y (TAT→TAC)	hlgC	gamma hemolysin component C
Reads supporting (aligned to +/- strand):  ref base T (33/18);  new base C (2/3);  total (35/24)
Fisher's exact test for biased strand distribution p-value = 3.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01

AATTAATTATTTACCTAAAAATAAAATTGAATCTACAAACGTGAGTCAGACATTAGGATACAATATCGGTGGTAATTTCCAATCAGCCCCATCACTCGGTGGTAATGGATCATTTAACTATTCTAAATCGATTAGCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGG‑GGCGTCAAAGCGAATTCATTCGCCACTGAATCAGGTCAAAAATCAGCCTTTGATAGCGATTTATTTGTAGGCTACAAACCT  >  CP000730/2544138‑2544414                                                                                                                                           |                                                                                                                                            aattaattATTTACCTAAAAATAAAATTGTATCTACAAACGTGAGTCAGACATTAGGATACAATATCGGTGGTAATTTCCAATCAGCCCCATCACTCGGTGGTAATGGATCATTTAACTATTCTAAATCGATTAGCtata                                                                                                                                            <  1:233801/140‑1 (MQ=255)  attaattaTTTACCTAAAAATAAAATTGAATCTACAAACGTGAGTCAGACATTAGGATACAATATCGGTGGTAATTTCCAATCAGCCCCATCACTCGGTGGTAATGGATCATTTAACTATTCTAAATCGATTAGCTATac                                                                                                                                           <  1:688202/140‑1 (MQ=255)              ccTAAAAATAAAATTGAATCTACAAACGTGAGTCAGACATTAGGATACAATATCGGTGGTAATTTCCAATCAGCCCCATCACTCGGTGGTAATGGATCATTTAACTATTCTAAATCGATTACCTATACACAACAAAATTa                                                                                                                               >  2:138296/1‑140 (MQ=255)                tAAAAATAAAATTGCACCTTCAAACGTGAGTCAGAGATTAGGATACAAGATCGGTGGTAATTTCCAATTAGCCCCATCACTCGGTGGTAATGGATCATTTAACTATTCTAAATCGATTAGCTATACACAACTAAAtgttg                                                                                                                             <  2:89681/140‑5 (MQ=255)                tAAAAATAAAATTGAATCTACAAACGTGAGTTAGATATTAGGATACAATATAGGTGGTAATTTCCAATCAGCCCCATCACTAGGTGGTAATGGATCATTTAACTATTCTAAATCGATTAGCTATACACAACAAATTTATg                                                                                                                             <  2:738691/140‑1 (MQ=255)                 aaaaaTAAAATTGAATCTACAAACGTGAGTCAGACATTAGGATACAATATCGGTGGTAATTTCCAATCAGCCCCATCACTCGGTGGTAATGGATCATTTAACTATTCTAAATCGATTAGCTATACACAACAAAATTATGt                                                                                                                            >  2:103423/1‑140 (MQ=255)                  aaaataaaatTGAATCTACAAACGTGAGTCAGACATTAGGATACAATATCGGTGGTAATTTCCAATCAGCCCCATCACTCGGTGGTAATGGATCATTTAACTATTCTAAATCGATTAGCCATACACAACAAAATTaagga                                                                                                                           >  2:264425/1‑136 (MQ=255)                       aaaatTGAATCTACAAACGTGAGTCAGACATTAGGATACAATATCGGTGGTAATTTCCAATCAGCCCCATCACTCGGTGGTAATGGATCATTTAACTATTCTAAATCGATTAGCTATACACAACAAAATTATGTAAGTGa                                                                                                                      >  1:500535/1‑140 (MQ=255)                       aaaatTGAATCTACAAACGTGAGTCAGACATTAGGATACAATATCGGGGAGAATTTCCAATCAGCCCCAACACTCGGAGGTAATGGATCATTTAACTATTCTAAATCGATTAGCTATACACCACAAAATTATGTAAGTGa                                                                                                                      >  1:400649/1‑140 (MQ=255)                        aaatTGAATCTACAAACGTGAGTCAGACATTAGGATACAATATCGGTGGTAATTTCCAATCAGCCCCATCACTCGGTGGTAATGGATCATTTAACTATTCTAAATCGATTAGCTATACACAACAAAATTATGTAAGTGaa                                                                                                                     <  1:757541/140‑1 (MQ=255)                                  tACAAACGTGAGTCAGACATTAGGATACAATATCGGTGGTAATTTCCAATCAGCCCCATCACTCGGTGGTAATGGATCATTTAACTATTCTAAATCGATTAGCTACACACAACAAAATTATGTAAGTGAAGTAGaacaac                                                                                                           >  2:367675/1‑140 (MQ=255)                                  tACAAACGTGAGTCAGACATTAGGATACAATATCGGTGGTAATTTCCAATCAGCCCCATCACTCGGTGGTAATGGATCATTTAACTATTCTAAATCGATTAGCTACACACAACAAAATTATGTAAGTGAAGTAGaacaac                                                                                                           >  2:325095/1‑140 (MQ=255)                                    cAAACGTGAGTCAGACATTAGGATACACTATCGGTGGTAATTTCCAATCAGCCCCATCACTCGGTGGTAATGGATCATTTAACTATTCTAAATCGATTAGCTATACACAACAAAATTATGTAAGTGAAGTAGAAcaacaa                                                                                                         <  1:172988/140‑1 (MQ=255)                                     aaaCGTGAGTCAGACATTAGGATACAATATCGGTGGTAATTTCCAATCAGCCCCATCACTCGGTGGTAATGGATCATTTAACTATACTAAATCGATTAGCTATACACAAAAAAATTATGTAAGTGAAGTAGAACaaaaaa                                                                                                        >  2:395057/1‑140 (MQ=255)                                       aCGTGAGTCAGACATTAGGATACAATATCGGTGGTAATTTCCAATCAGCCCCATCACTCGGTGGTAATGGATCATTTAACTATTCTAAATCGATTAGCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAAc                                                                                                      <  1:80104/140‑1 (MQ=255)                                          tGAGTCAGACATTAGGATACAATATCGGTGGTAATTTCCAATCAGCCCCATCACACGGTGGTAATGGATCATTTAACTATTCTAAATCGATTAGCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCa                                                                                                   >  1:507019/1‑140 (MQ=255)                                             gTCAGACATTAGGATACAATATCGGTGGTAATTTCCAATCAGCCCCATCACTCGGTGGTAATGGATCATTTAACTATTCTAAATCGATTAGCTATACACAACAAAATTATGTAAGTAAAGTCGAACAACAAAACTCaaaa                                                                                                >  2:581570/1‑140 (MQ=255)                                                    aTTAGGATACAATATCGGTGGTAATTTCCAATCAGCCCCATCACTCGGTGGTAATGGATCATTTAACTATTCTAAATCGATTAGCTACACACAACAAAATTATGTACGAGAAGAAGAACAACAAAACTCAAAAAGTGttt                                                                                         <  1:367675/140‑1 (MQ=255)                                                    aTTAGGATACAATATCGGTGGTAATTTCCAATCAGCCCCATCACTCGGTGGTAATGGATCATTTAACTATTCTAAATCGATTAGCTACACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGttt                                                                                         <  1:325095/140‑1 (MQ=255)                                                     ttataaTACAAAATCGGTGGTAATCTCCAATCAGCCCCATCACTCGGTGGTAATGGATCATTTAACTATTCTAAATCAATTAGCCATACACAACAAAATTAGGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGtttt                                                                                        <  1:64590/135‑1 (MQ=255)                                                     ttAGGATACGATATCGGTAGTAATTTCTAATCAGCCCCATCACTCTGTTGTAATGGATCATTTAACTATTCTAAAACCATCAGCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGtttt                                                                                        <  1:441087/140‑1 (MQ=255)                                                      tAGGATACAAAATCGGTGGTAATTTCCAATCAACCCCATCACTCGGTGGTAATGGATCATTTAACTATTCTAAATCGAATAGCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTa                                                                                       <  1:124554/140‑1 (MQ=255)                                                            aCAATATCGGTGGTAATTTCCAATCAGCCCCATCACTCGGTGGTAATGGATCATTTAACGATGCTAAATCGATTAGCTATACACAACAAAAAGATGTAATTTAAGAATAAAAACAAAACTCagaaaatcatgtattcgac                                                                                 >  1:343443/1‑126 (MQ=255)                                                                 atCGGTGGTAGTTTCCAATCAGCCCCATCACTCGGTGGTAATGGATCATTTAACTATTCTAAATCGATTAGCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGG‑GGCGTTaa                                                                           >  2:514077/1‑140 (MQ=255)                                                                 atCGGTGGTAATTTCCAATCAGCCCCATCACTCGGTGGTAATGGATCATTTAACTATTCTAAATCGATTAGCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTGTCTGG‑GGCGTTaa                                                                           >  2:744559/1‑140 (MQ=255)                                                                 atCGGTGGTAATTTCCAATCAGCCCCATCACTCGGTGGTAATGGATCATTTAACTATTATAAATCGATTAGCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGG‑GGCGTTaa                                                                           >  2:66587/1‑140 (MQ=255)                                                                  tCGGTGGTAATTTCCAATCAGCCCCATCACTCGGTGGTAATGGATCATTTAACTATTCTAAATCGATTAGCTATACACAACAAAATTAAGTAAGTGAAGTAGAACAACAACACTCAAAAAGTGTTTTATGG‑GACGTTaaa                                                                          >  2:31044/1‑140 (MQ=255)                                                                           aTTTCCAATCAGCCCCATCACTCGGTGGTAATGGATCATTTAACTATTCTAAATCGATTAGCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGG‑GGCGTTAAAGCGAattca                                                                 >  2:451060/1‑140 (MQ=255)                                                                           aTTTCCAATCAGCCCCATCACTCGGTGGTAATGGATCATTTAACTATTCTAAATCGATTAGCTATACACAACAAAATTATGTAAGTGAAGTAGAAAAACAAAACTCAAAAAGGGTTTTATGG‑GGCGTTAAAGCGAattaa                                                                 >  2:20823/1‑138 (MQ=255)                                                                             ttCCAATCAGCCCCATCACTCGGTGGTAATGGATCATTTAACTATTCTAAATCGATTAGCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACCAAACTCAAAAAGTGTTTTATGG‑GGCGTTAAAGCGAattcatt                                                               >  2:296749/1‑140 (MQ=255)                                                                             ttCCAATCAGCCCCATCACTCGGTGGTAATGGATCATTTAACTATTCTAAATCGATGAGCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGCTTTATGG‑GGCGTTACAGCGAattcatt                                                               >  2:442444/1‑140 (MQ=255)                                                                              tCCAATCAGCCCCATCACTCGGTGGTAATGGATCATTTAACTATTCTAAATCGATTAGCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGG‑GGCGTTAAAGCGAattcattc                                                              >  2:715276/1‑140 (MQ=255)                                                                              tCCAATCAGCCCCATCACTCGGTGGTAATGGATCATTTAACTATTCTAAATCGATTAGCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGG‑GGCGTTAAAGCGAattcattc                                                              >  2:729093/1‑140 (MQ=255)                                                                                   tCAGCCCCATCACTCGGTGGTAATGGATCATTTAACTATTCTAAATCGATTAGCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGG‑GGCGTTAAAGCGAATTCATTCGCCAc                                                         >  2:752121/1‑140 (MQ=255)                                                                                   tCAGCCCCATCACTCGGTGGTAATGGATCATTTAACTATTCTAAATCGATTAGCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGG‑GGCGTTAAAGCGAATTCATTCGCCAc                                                         >  1:194888/1‑140 (MQ=255)                                                                                   tCAGCCCCATCACTCGGTGGTAATGGATCATTTAACTATTCTAAATCGATTAGCTATACACAACAAAATTATGTAAGTGAAGTAAAACAACAAAACTCAAAAAGTGTTTTATGG‑GGCGTTAAAGCGAATTCATTCGCCAc                                                         >  1:194937/1‑140 (MQ=255)                                                                                     aGCCCCATCACTCGGTGGTAATGGATCATTTAACTATTCTAAATCGATTAGCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAATTGTTTTATGG‑GGCGTTAAAGCGAATTCATTCGCCACTg                                                       >  2:169903/1‑140 (MQ=255)                                                                                     aGCCCCATCACTCGGTGGTAATGGATCATTTAACTATTCTAAATCGATTAGCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGG‑GGCGTTAAAGCGAATTCATTCGCCACTg                                                       <  2:663571/140‑1 (MQ=255)                                                                                        cccATCACTCGGTGGTAATGGATCATTTAACTATTCTAAATCGATTAGCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGG‑GGCGTTAAAGCGAATTCATTCGCCACTGAAt                                                    >  2:362631/1‑140 (MQ=255)                                                                                          cATCACTCGGTGGTAATGGATCATTTAACTATTCTAAATCGATTAGCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGG‑GGCGTTAAAGCGAATTCATTCGCCACTGAATCa                                                  >  1:759357/1‑140 (MQ=255)                                                                                             tacccGGTAGTAATGGAACAATTAACTATTCAAAATCAATCACCAAAACAAAACCTAAATATATAAAACAAATAAAAAAATAAAAATAAAAAAGTATTTTATGG‑CGCGTTAAAGCTAATTCATTCGCCACTGAATCAGGt                                               <  2:283898/136‑1 (MQ=255)                                                                                                  ggtggtAATGGATCATTTAACTATTCTAAATCGATTAGCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAATGTTTTATGG‑GGCGTTAAAGCGAATTCATTCGCCACTGAATCAGGTCaaaa                                          >  1:102073/1‑140 (MQ=255)                                                                                                     ggtAATGGATCATTTAACTATTCTAAATCGATTAGCTATACACAACAAATTCCTTTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGG‑GGCGTTAAAGCGAATTCATTCGCCACTGAATCAGGTCAAAAATc                                       <  1:590032/140‑1 (MQ=255)                                                                                                         aTGGATCATTTAACTATTCTAAATCGATTAGCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGG‑GGCGTTAAAGCGAATTCATTCGCCACTGAATCAGGTCAAAAATCAGcc                                   >  1:96729/1‑140 (MQ=255)                                                                                                         aTGGATCATTTAACTATTCTAAATCGATTAGCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGG‑GGCGTTAAAGCGAATTCATTCGCCACTGAATCAGGTCAAAAATCAGcc                                   <  1:567271/140‑1 (MQ=255)                                                                                                           ggATCATTTAACTATTCTAAATCGATTAGCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGG‑GGCGTTAAAGAGAATTAATACGCCCCTGAATCAGAACAAAAagcagcata                                 >  2:716535/1‑137 (MQ=255)                                                                                                                   tAACTATTCTAAATCGATTAGCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGG‑GGCGTTAAAGCGAATTCATTCGCCACTGAATCAGGTCAAAAATCAGCCTTTGATAGCg                         >  1:454680/1‑140 (MQ=255)                                                                                                                    aaCTATTCTAAATCGATTAGCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGG‑GGCGTTAAAGCGAATTCATTCGCCACTGAATCAGGTCAAAAATCAGCCTTTGATAGCGa                        <  1:598544/140‑1 (MQ=255)                                                                                                                    aaCTATTCTAAATCGATTAGCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGG‑GGCGAATAAGCGAATTCATTCGCCACTGAATCAGGTCAAAAATCAGCCTTTGATAGCGa                        >  1:589925/1‑140 (MQ=255)                                                                                                                        aatCTAAATCGATTAACAAAACACAACAAAATTATGTAAGTGAAGTAGAAAAAAAAAAAAAAAAAAGTGTTTTATGG‑GGCGTTAAAGCGAATTCATTCGCCACTGAATCAGGTCAAAAATCAGCCTTTGATAGCGattta                    <  2:446256/138‑1 (MQ=255)                                                                                                                        aTTCTAAATCGATTAGCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAATAAGTGTTTTATTG‑GGCGTTAAAGCGAATTCATTCGCCACTGAATCAGGTCAAAAATCAGCCTTTGATAGCGattta                    <  2:428890/140‑1 (MQ=255)                                                                                                                         ttCTAAATCGATTAGCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGG‑GGCGTTAAAGCGAATTCATACGCCACTGAATCAGGTCAAAAATCAGCCTTTGATGGCGatttat                   >  2:171786/1‑140 (MQ=255)                                                                                                                          tCTAAATCGATTATCTTTCCACAACAAAATTATGTAAGTGAAGTATAATTACTTCACTCTTAAAGTGTTTTATGG‑GGCGTTAAAGTGAATTTATTCGTCACTGAATCAGGTCAAAAATCAGCCTTTGATAGCGatttatt                  <  1:670580/140‑1 (MQ=255)                                                                                                                                 cGATTAGCTACACAGAACCAAGTTATACAAGAGAAGNAGAACAACAACCCTCAAGAAGCATCTAACGGCGGTGT‑AAAGCTAATTCATTGGACAAAAAATCAAAACAAAAATCAGCCTTTGATAGCGATTTATTTGTAGGc           <  2:1933/140‑1 (MQ=255)                                                                                                                                  gATTAGCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGG‑GGCGTTAAAGCGAATTCATTCGCCACTGAATCAGGTCAAAAATCAGCCTTTGATAGCGATTTATTTGTAGGCt          <  1:677040/140‑1 (MQ=255)                                                                                                                                   aTTAGCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGG‑GGCGTTAAAGCGAATTCATTCGCCACTGAATCAGGTCAAAAATCAGCCTTTGATAGCGATTTATTTGTAGGCTa         <  1:339599/140‑1 (MQ=255)                                                                                                                                   aTTAGCTAGACACAACTAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGG‑GGCGTTAAAGCGAATTCATTCGCCACTGAATCAGGTCAAAAATCAGCCTTTGATAGCGATTTATTTGTAGGCTa         <  2:392039/140‑1 (MQ=255)                                                                                                                                     tAGCTATACAAAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGG‑GGCGTCAAAGCGAATTCATTCGCCACTGAATCAGGTCAAAAATCAGCCTTTGATAGCGATTTATTTGTAGGCTACa       <  1:427427/140‑1 (MQ=255)                                                                                                                                       gCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGG‑GGCGTTAAAGCGCATTACTTCGCCACTGCATCCGGACACAACTCAGACTTGGATAGGGATTTAATTGTAGGCTACaaa     >  2:522635/1‑140 (MQ=255)                                                                                                                                       gCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGG‑GGCGTTAAAGCGAATTCATTCGCCAATGCATCAGGTCAGCAATCGGCCTTTGAGAGCGAGTTATCTGTAGGCTACaag     >  2:522654/1‑139 (MQ=255)                                                                                                                                         tataCACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGG‑GGCGTCAAAGCGAATTCATTCGCCACTGAATCAGGTCAAAAATCAGCCTTTGATAGCGATTTATTTGTAGGCTACAAAcc   >  2:289957/1‑140 (MQ=255)                                                                                                                                          ataCACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGG‑GGCGTTAAAACGAATTCATTCGCCACTAAATAAGGTCAAAAAGCAGCCATTGATAGCGGTTTATTTGTAGCCTACAAACCt  >  1:235177/1‑140 (MQ=255)                                                                                                                                          ataCACAACAAAATTATGTAAGTGAACTAGAACAACATAAAGCAAACAGTGTTTTATGG‑GGCGTTAAAGCGAATTCATTCGACACTGAATCAGGTCACAAATCAGCCTTTGATAGCGATTTATTTGTAGGCTACAAACCt  <  2:728629/140‑1 (MQ=255)                                                                                                                                           |                                                                                                                                            AATTAATTATTTACCTAAAAATAAAATTGAATCTACAAACGTGAGTCAGACATTAGGATACAATATCGGTGGTAATTTCCAATCAGCCCCATCACTCGGTGGTAATGGATCATTTAACTATTCTAAATCGATTAGCTATACACAACAAAATTATGTAAGTGAAGTAGAACAACAAAACTCAAAAAGTGTTTTATGG‑GGCGTCAAAGCGAATTCATTCGCCACTGAATCAGGTCAAAAATCAGCCTTTGATAGCGATTTATTTGTAGGCTACAAACCT  >  CP000730/2544138‑2544414

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑