Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	2,384,414	T→C	7.3%	Y217C (TAT→TGT)	fmhB ←	peptidoglycan pentaglycine interpeptide biosynthesis protein FmhB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	2,384,414	0	T	C	7.3%	170.4 / 4.2	65	Y217C (TAT→TGT)	fmhB	peptidoglycan pentaglycine interpeptide biosynthesis protein FmhB
Reads supporting (aligned to +/- strand):  ref base T (28/32);  new base C (2/3);  total (30/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.12e-01

CAGATGTTTTCATCTTCTGCTGCCATTTAGCAATTTCGGCATGAAGTTCATTCAATTCTTGATTTACTTTCGCTATATTTTCTTTTGGATCCAACTTTACTAAAAATAGTTCAGCATCTCCATCTTCATGCAACGCATCATAAATATTTTCAAAGTAACTAATATCACGCG‑TTAAGAAGCCATCGCGTTCCCCAGTGATTTTCATTAACTCAGCAAATGTTTTTAAACCTTCTCTATCAGATCGTTCTACTGTCGTACCTCGCTTTAAAGCCAAGC  >  CP000730/2384274‑2384549                                                                                                                                             |                                                                                                                                         cAGATGTTTTCATCTTCTGCTGCCATTTAGCAATTTCGGCATGAAGTTCATTCAATTCTTGATTTACTTTCGCTATATTTTCTTTTGGATCCAACTTTACTAAAAATAGTTCAGCATCTCCATCTTCATGCAACGcctcat                                                                                                                                          >  2:610804/1‑141 (MQ=255) cAGATGTTTTCATCTTCTGCTGCCATTTAGCAATTTCGGCATGAAGTTCATTCAATTCTTGATTTACTTTCGCTATATTTTCTTTTGGATCCAACTTTACTAAAAATAGTTCAGCATCTCCATCTTCATGCAACGcatcat                                                                                                                                          <  1:584642/141‑1 (MQ=255) cAGATGTTTTCATCTTCTGCTGCCATTTAGCAATTTCGGCATGAAGTTCATTCAATTCTTGATTTACTTTCGCTATATTTTCTTTTGGATCCAACTTTACTAAAAATAGTTCAGCATCTCCATCTTCATGCAACGcatcat                                                                                                                                          <  1:611595/141‑1 (MQ=255)   gATGTTTTCATCTTCTGCTGCCATTTAGCAATTTCGGCATGAAGTTCATTCAATTCTTGATTTACTTTCGCTATATTTTCTTTTGGATCCAACTTTACTAAAAATAGTTCAGCATCTCCATCTTCATGCAACGCATCATaa                                                                                                                                        <  1:404225/141‑1 (MQ=255)   gATGTTTTCATCTTCTGCTGCCATTTAGCAATTTCGGCATGAAGTTCATTCAATTCTTGATTTACTTTCGCTATATTTTCTTTTGGATCCAACTTTACTAAAAATAGTTCAGCATCTCCATCTTCATGCAACGCATCATaa                                                                                                                                        <  1:429698/141‑1 (MQ=255)     tGTTTTCATCTTCTGCTGCCATTTAGCAATTTCGGCATGAAGTTCATTCAATTCTTGATTTACTTTCGCTATATTTTCTTTTGGATCCAACTTTACTAAAAATAGTTCAGCATCTCCATCTTCATGCAACGCATCATAAat                                                                                                                                      <  2:404045/141‑1 (MQ=255)     tGTCTTCATCTTCTGCTGCCATTTAGCAATTTCGGCATGAAGTTCATTCAATTCTTGATTTACTTTCGCTATATTTTCTTTTGGATCCAACTTTACTAAAAATAGTTCAGCATCTCCATCTTCATGCAACGCATCATAAat                                                                                                                                      <  1:223702/141‑1 (MQ=255)      gTTTTCATCTTCTGCTGCCATTTAGCAATTTCGGCATGAAGTTCATTCAATTCTTGATTTACTTTCGCTATATTTTCTTTTGGATCCAACTTTACTAAAAATAGTTCAGCATCTCCATCTTCATGCAACGCATCATAAata                                                                                                                                     >  2:407194/1‑141 (MQ=255)       tattCATATTCTGCTGCCATTTAGCAATTTCGGCATGAAGTTCATTCAATTCTTGATTTACTTTCGCTATATTTTCTTTTGGATCCACCTTTACTAAAAATAGTTCACCATCCCCATCTTCATCCAACGCATGATCAatat                                                                                                                                    >  2:464457/3‑141 (MQ=255)         ttCATCTTCTTCTGCCATTTAGCAATTTCGGAATGAAGTTCATTCAAGCCTTGATTTACTTTCGCTATATTTTCTTTTGGATCCAACTTTACTAAAAACAGTTCACCATCTCCATCTTCATGCAACGCATCATAAATAttt                                                                                                                                  <  2:22968/141‑1 (MQ=255)         ttCATCTTCTGCTGGCATTTAGCAATTTCGGCATGAAGTTCATTCAATTCTTGATTTACTTTCGCTATATTTTCTTTTGGATCCAACTTTACTAAAAATAGTTCAGCATCTCCATCTTCATGCAACGCATCATAAATAttt                                                                                                                                  <  2:584443/141‑1 (MQ=255)         ttCATCTTCTGCTGCCATTTAGCAATTTCGGCATGAAGTTCATTCAATTCTTGATTTACTTTCGCTATATTTTCTTTTGGATCCAACTTTACTAAAAATAGTTCAGCATCTCCATCTTCATGCAACGCATCATAAATAttt                                                                                                                                  <  1:622466/141‑1 (MQ=255)                tctGCTGCCATTTAGCAATTTCGGCATGAAGTTCATTCAATTCTTGATTTACTTTCGCTATATTTTCTTTTGGATCCAACTTTACTAAAAATAGTTCAGCATCTCCATCTTCATGCAACGCATCATAAATATTTTCAAAGt                                                                                                                           <  1:97049/141‑1 (MQ=255)                   gctgcCATTTAGCAATTTCGGCATGAAGTTCATTCAATTCTTGATTTACTTTCGCTATATTTTCTTTTGGATCCAACTTTACTAAAAATAGTTCAGCATCTCCATCTTCATGCAACGCATCATAAATATTTTCAAAGTAAc                                                                                                                        <  1:184048/141‑1 (MQ=255)                     tgcCATTTAGCAATTTCGGCATGAAGTTCATTCAATTCTTGATTTACTTTCGCTATATTTTCTTTTGGATCCAACTTTACTAAAAATAGTTCAGCATCTCCATCTTCATGCAACGCCTCATAAATATTTTCAACGTAACTa                                                                                                                      >  2:60344/1‑141 (MQ=255)                      gcCATTTAGCAATTTCGGCATGAAGTTCATTCAATTCTTGATTTACTTTCGCTATATTTTCTTTTGGATCCAACTTTACTAAAAATAGTTCAGCATCTCCATCTGCATGCAACGCATCATAAATATTTTCAAAGTAACTaa                                                                                                                     >  1:421334/1‑141 (MQ=255)                         aTTTAGCCATTTCGGCATGAAATTCATTTAATTTCAGCTTTATATTCTCGATATACTCGTAGTGATCGATGTTGGCAAAAGATACTTCATCATCTGCGTCTACATGCAACGCATCATAAATATTTTCAAAGTAACTAatat                                                                                                                  <  2:65867/141‑1 (MQ=255)                                aaTTTCGGCATGAAGTTCATTCAATTCTTGATTTACTTTCGCTATATTTTCTTTTGGATCCAACTTTACTAAAAATAGTTCAGCATCTCCATCTTCATGCAACGCATCACAAATATTTTCAAAGTAACTAATATCACGCG‑t                                                                                                          >  2:398170/1‑141 (MQ=255)                                aaTTTCGGCATGAAGTTCATTCAATTCTTGATTTACTTTCGCTATATTTTCTTTTGGATCCAACTTTACTAAAAATAGTTCAGCATCTCCATCTTCATGCAACGCATCACAAATATTTTCAAAGTAACTAATATCACGCG‑t                                                                                                          >  2:547072/1‑141 (MQ=255)                                    tCGGCTAGAAGTTCATGCCATTCTGGATTCTCTTTCGCTATCTTTTCTTTTGGATCCAACTTTACCCAAACTAGTTCCGCCCCCCCCTCTTCGTGCAACGCATCACAAATCTGTTCCAAGCAGCTAATATCACGCG‑TTaag                                                                                                      <  2:264614/141‑1 (MQ=255)                                     cGGCATGAAGTTCATTCAATTCTTGATTTACTTTCGCTATATTTTCTTTTGGATCCAACTTTACTAAAAATAGTTCAGCATCTCCATCTTCATGCAACGCATCATAAATATTTTCAAAGTAACTAATATCACGCG‑TTaaga                                                                                                     <  2:740374/141‑1 (MQ=255)                                        cATGAAGTTCATTCAATTCTTGATTTACTTTCGCTATATTTTCTTTTGGATCCAACTTTACTAAAAATAGTTCAGCATCTCCATCTTCATGCAACGCATCATAAATATTTTCAAAGTAACTAATATCACGCG‑TTAAGAAGc                                                                                                  <  1:289586/141‑1 (MQ=255)                                             aGTTCATTCAATTCTTGATTTACTTTCGCTATATTTTCTTTTGGATCCAACTTTACTAAAAATAGTTCAGCATCTCCATCTTCATGCAACGCATCATAAATATTTTCAAAGTAACTAATATCACGCG‑TTAAGAAGCCATcc                                                                                             >  1:118490/1‑140 (MQ=255)                                               ttcattcaATTCTTGATTTACTTTCGCTATATTTTCTTTTGGATCCAACTTTACTAAAAATAGTTCAGCATCTCCATCTTCATGCAACGCATCATAAATATTTTCAAAGTAACTAATATCACACG‑TTAAGAAGCCATggcg                                                                                           >  2:438710/1‑141 (MQ=255)                                                tcattcaATTCTTGATTTACTTTCGCTATATTTTCTTTTGGATCCAACTTGACTAAAAATAGTTCAGCATCTCCATCTTCATGCAACGCATCATAAATATTTTCAAAGTAACTAATATCACGCG‑TTAAGAAGCCATCGCGt                                                                                          >  1:213613/1‑141 (MQ=255)                                                 cattcaATTCTTGATTTACTTTCGCTATATTTTCTTTTGGATCCAACTTTACTAAAAATAGTTCAGCATCTCCATCTTCATGCAACGCATCATAAATATTTTCAAAGTAACTAATATCACGCG‑TTAAGAAGCCATCGCGtt                                                                                         >  1:762954/1‑141 (MQ=255)                                                          cTTGATTTACTTTCGCTATATTTTCTTTTGGATCCAACTTTACTAAAAATAGTTCAGCATCTCCATCTTCATGCAACGCATCATAAATATTTTCAAAGTAACTAATATCACGCG‑TTAAGAAGCCATCGCGTTCCCCAGTGa                                                                                <  1:305849/141‑1 (MQ=255)                                                               tttACTTTCGCTATATTTTCTTTTGGATCCAACTTTACTAAAAATAGTTCAGCATCTCCATCTTCATGCAACGCATCATAAATATTTTCAAAGTAACTAATATCACGCG‑TTAAGAAGCCATCGCTTTCCCCCGTGATTTTc                                                                           >  2:433393/1‑141 (MQ=255)                                                                ttACTTTCGCTATATTTTCTTTTGGATCCAACTTTACTAAAAATAGTTCAGCATCTCCATCTTCATGCAACGCATCATAAATATTTTCAAAGTAACTAATATCACGCG‑TTAAGAAGCCATCGCGTTCCCCAGTGATTTTCa                                                                          <  2:583694/141‑1 (MQ=255)                                                                 tACTTTCGCTATATTTTCTTTTGGATCCAACTTTACTAAAAATAGTTCAGCATCTCCATCTTCATGCAACGCATCATAAATATTTTCAAAGTAACTAATATCACGCG‑TTAAGAAGCCATCGCGTTCCCCAGTGATTTTCAt                                                                         >  2:433043/1‑141 (MQ=255)                                                                    tttCGCTATATTTTCTTTTGGATCCAACTTTACTAAAAATAGTTCAGCATCTCCATCTTCATGCAACGCATCATAAATATTTTCAAAGTAACTAATATCACGCG‑TTAAGAAGCCATCGCGTTCCCCAGTGATTTTCATTaa                                                                      >  2:629829/1‑141 (MQ=255)                                                                         cTATATTTTCTTTTGGATCCAACTTTACTAAAAATAGTTCAGCATCTCCATCTTCATGCAACGCATCACAAATATTTTCAAAGTAACTAATATCACGCG‑TTAAGAAGCCATCGCGTTCCCCAGTGATTTTCATTAACTCAg                                                                 <  1:398170/141‑1 (MQ=255)                                                                         cTATATTTTCTTTTGGATCCAACTTTACTAAAAATAGTTCAGCATCTCCATCTTCATGCAACGCATCACAAATATTTTCAAAGTAACTAATATCACGCG‑TTAAGAAGCCATCGCGTTCCCCAGTGATTTTCATTAACTCAg                                                                 <  1:547072/141‑1 (MQ=255)                                                                          tataTTTTCTTTTGGATCCAACTTTTCTAAAAATAGTTCAGCATCTCCATCTTCATGCAACGCATCATAAATATTTTCAAAGTAACTAATATCACGGC‑TTAAGAAGCCAGCGCGTTCCCCAGTGATTTTCATTAACTCAGc                                                                <  1:332859/141‑1 (MQ=255)                                                                            tatTTGCTGTTGGCTCCAACTTTACTAAAAATAGTTCATCATCTCCATCTTCATGCAACGCATCATAAATATTTTCAAAGTAACTAATATCACGCG‑TTAAGAAGCCATCGCGTTCCCCAGTGATTTTCATTAACTCAGCaa                                                              <  2:630487/141‑1 (MQ=255)                                                                             atTTTCTTTTGGATCCAACTTTACTAAAAATAGTTCAGCATCTCCATCTTCCTCATACACAGGATAACTATTTTCAAAGTAACTAATATCACGCG‑TTAAGAAGCCATCGCGTTCCCCAGTGATTTTCATTAACTCAGCaaa                                                             >  1:143584/1‑141 (MQ=255)                                                                             atTTTCTTTTGGATCCAACTTTACTAAAAATAGTTCAGCATCTCCATCTTCATGCAACGCATCATAAATATTTTCAAAGTAACTAATATCACGCG‑TTAAGAAGCCATCGCGTTCCCCAGTGATTTTCATTAACTCAGCaaa                                                             >  2:208885/1‑141 (MQ=255)                                                                                    tttGGATCCAACTTTACTAAAAATAGTTCAGCATCTCCATCTTCATGCAACGCATCATAAATATTTTCAAAGTAACTAATATCACGCG‑TTAAGAAGCCATCGCGTTCCCCAGTGATTTTCATTAACTCAGCAAATGttttt                                                      <  1:621307/141‑1 (MQ=255)                                                                                     ttGGATCCAACTTTACTAAAAATAGTTCAGCATCTCCATCTTCATGCAACGCATCATAAATATTTTCAAAGTAACTAATATCACGCG‑TTAAGAAGCCATCGCGTTCCCCAGTGATTTTCATTAACTCAGCAAATGTTTTTa                                                     >  2:276734/1‑141 (MQ=255)                                                                                      tGGATCCAACTTTACTAAAAATAGTTCAGCATCTCCATCTTCATGCAACGCATCATAAATATTTTCAAAGTAACTAATATCACGCG‑TTAAGAAGCCATCGCGTTCCCCAGTGATTTTCATTAACTCAGCAAATGTTTTTaa                                                    >  2:373663/1‑141 (MQ=255)                                                                                        gATGCAACTTTACTAAAAATAGTTCAGCATCTCCATCTTCATGCAACGCATCATAAATATTTTCAAAGTAACTAATATCACGCG‑TTAAGAAGCCATCGCGTTCCCCAGTGATTTTCATTAACTCAGCAAATGTTTTTAAAc                                                  <  2:394067/141‑1 (MQ=255)                                                                                         gtCCAACTTTACTAAAAATAGTTCAGCATCTCCATCTTCATGCAACGCATCATAAATATTTTCAAAGTAACTAATATCACGCG‑TTAAGAAGCCATCGCGTTCCCCAGTGATTTTCATTAACTCAGCAAATGTTTTTAAAcc                                                 <  2:187236/140‑1 (MQ=255)                                                                                            cAACTTTACTAAAAATAGTTCAGCATCTCCATCTTCATGCAACGCATCATAAATATTTTCAAAGTAACTAATATCACGCG‑TTAAGAAGCCATCGCGTTCCCCAGTGATTTTCATTAACTCAGCAAATGTTTTTAAACCTtc                                              >  1:735162/1‑141 (MQ=255)                                                                                              aCTTTCCTACAAATAGTTCAGCATTTCCATCTTCATGCAACGCATCATAAATATTTTCAAAGTAACTAATATCACGCG‑TTAAGAAGCCATCGCGTTCCCCAGTGATTTTCATTAACTCAGCAAATGTTTTTAAACCTtctc                                            <  1:633570/141‑1 (MQ=255)                                                                                              aCTTTACTAAAAATAGTTCAGCATCTCCATCTTCATGCAACGCATCATAAATATTTTCAAAGTAACTAATATCACGCG‑TTAAGAAGCCATCGCGTTCCCCAGTGATTTTCATTAACTCAGCAAATGTTTTTAAACCTtctc                                            <  2:39107/141‑1 (MQ=255)                                                                                                  tACTAAAAATAGTTCAGCATCTCCATCTTCATGCAACGCATCATAAATATTTTCAAAGTAACTAATATCACGCG‑TTAAGAAGCCATCGCGTTCCCCAGGGATTTTCATTAACTCAGCAAATGTTTTTAAACCTTCTCTATc                                        <  1:25515/141‑1 (MQ=255)                                                                                                   aCTAAAAATAGTTCAGCATCTCCATCTTCATGCAACGCATCATAAATATTTTCAAAGTAACTAATATCACGCG‑TTAAGAAGCCATCGCGTTCCCCAGTGATTTTCATTAACTCAGCAAATGTTTTTAAACCTTCTCTATCa                                       <  1:309682/141‑1 (MQ=255)                                                                                                   aCTAAAAATAGTTCAGCATCTCCATCTTCATGCAACGCATCATAAATATTTTCAAAGTAACTAATATCACGCG‑TTAAGAAGCCATCGCGTTCCCCAGTGATTTTCATTAACTCAGCAAATGTTTTTAAACCTTCTCTATCa                                       >  2:582320/1‑141 (MQ=255)                                                                                                      aaaaaTAGTTCAGCATCTCCATCTTCATGCAACGCATCATAAAACTACTATCGTTAACGACTACCA‑GCGCAGAATAAGCCATCGCGTTCCCCAGTGATTTGCAGGAACTCAGCAAATGGTTTTAAACCTTCTCTATCAGAt                                    >  1:752431/1‑141 (MQ=255)                                                                                                         aaTAGTTCAGCATCTCCATCTTCATGCAACGCATCATAAATATTTTCAAAGTAACTAATATCACGCG‑TTAAGAAGCCATCGCGTTCCCCAGTGATTTTCATTAACTCAGCAAATGTTTTTAAACCTTCTCTATCAGATCGt                                 <  2:349910/141‑1 (MQ=255)                                                                                                         aaTAGTTCAGCATCTCCATCTTCATGCAACGCATCATAAATATTTTCAAAGTAACTAATATCACGCG‑TTAAGAAGCCATCGCGTTCCCCAGTGATTTTCATTAACTCAGCAAATGTTTTTAAACCTTCTCTATCAGATAGt                                 >  2:194236/1‑141 (MQ=255)                                                                                                            aGTTCAGCATCTCCATCTTCATGCAACGCATCATAAATATTTTCAAAGTAACTAATATCACGCG‑TTAAGAAGCCATCGCGTTCCCCAGTGATTTTCATTAACTCAGCAAATGTTTTTAAACCTTCTCTATCAGATCGTTCt                              <  1:213765/141‑1 (MQ=255)                                                                                                             gTTCAGCATCTCCATCTTCATGCAACGCATCATAAATATTTTCAAAGTAACTAATATCACGCG‑TTAAGAAGCCATCGCGTTCCCCAGTGATTTTCATTAACTCAGCAAATGTTTTTAAACCTTCTCTATCAGATCGTTCTa                             >  2:298555/1‑141 (MQ=255)                                                                                                                cAGCATCTCCATCTTCATGCAACGCATCATAAATATTTTCAAAGTAACTAATATCACGCG‑TTAAGAAGCCATCGCGTTCCCCAGTGATTTTCATTAACTCAGCAAATGTTTTTAAACCTTCTCTATCAGATCGTTCTACTg                          >  1:368298/1‑141 (MQ=255)                                                                                                                 aGCATCTCCATCTTCATGCAGAGAAGCATAAATATTTTCAAAGTAACTAATATCACGCG‑TTAAGAAGCCATCGCGTTCCCCAGTGATTTTCATTAACTCAGCAAATGTTTTTAAACCTTCTCTATCAGATCGTTCTACTGt                         >  1:559685/1‑141 (MQ=255)                                                                                                                 aGCATCTCCATCTTCATGCAACGCATCATAAATATTTTCAAAGTAACTAATATCACGCG‑TTAAGAAGCCATCGCGTTCCCCAGTGATTTTCATTAACTCAGCAAATGTTTTTAAACCTTCTCTATCAGATCGTTCTACTGt                         <  1:248388/141‑1 (MQ=255)                                                                                                                    aTCTCCATCTTCATGCAACGCATCATAAATATTTTCAAAGTAACTAATATCACGCG‑TTAAGAAGCCATCGCGTTCCCCAGTGATTTTCATTAACTCAGCAAATGTTTTTAAACCTTCTCTATCAGATCGTTCTACTGTCGt                      <  1:194168/141‑1 (MQ=255)                                                                                                                    aTCTCCATCTTCATGCAACGCATCATAAATATTTTCAAAGTAACTAATATCACGCG‑TTAAGAAGCCATCGCGTTCCCCAGTGATTTTCATTAACTCAGCAAATGTTTTTAAACCTTCTCTATCAGATCGTTCTACTGTCGt                      <  1:193964/141‑1 (MQ=255)                                                                                                                       tcCATCTTCATGCAACGCATCATAAATATTTTCAAAGTAACTAATATCACGCG‑TTAAGAAGCCATCGCGTTCCCCAGTGATTTTCATTAACTCAGCAAATGTTTTTAAACCTTCTCTATCAGATCGTTCTACTGTCGTAcc                   >  1:102961/1‑141 (MQ=255)                                                                                                                       tcCATCTTCATGCAACGCATCATAAATATTTTCAAAGTAACTAATATCACGCG‑TTAAGAAGCCATCGCGTTCCCCAGTGATTTTCATTAACTCAGCAAATGTTTTTAAACCTTCTCTATCAGATCGTTCTACTGTCGTAcc                   <  1:407194/141‑1 (MQ=255)                                                                                                                       tcCATCTTCATGCAACGCATCATAAATATTTTCAAAGTAACTAATATCACGCG‑TTAAGAAGCCATCGCGTTCCCCAGTGATTTTCATTAACTCAGCAAATGTTTTTAAACCTTCTCTATCAGATCGTTCTACTGTCGTAcc                   >  1:233013/1‑141 (MQ=255)                                                                                                                       tcCATCTTCATGCAACGCATCATAAATATTTTCAAAGTAACTAATATCACGCG‑TTAAGAAGCCATCGCGTTCCCCAGTGATTTTCATTAACTCAGCAAATGTTTTTAAACCTTCTCTATCAGATCGTTCTACTGTCGTAcc                   >  1:655822/1‑141 (MQ=255)                                                                                                                       tcCATCTTCATGCAACGCATCATAAATATTTTCAAAGTAACTAATATCACGCG‑CTAAGAAGCCATCGCGTTCCCCAGTGATTTTCATTCACTCAGCAAATGTTTTTAAACCTTCTCTATCAGATCGTTCTACTGTCGTAcc                   >  2:375334/1‑141 (MQ=255)                                                                                                                           tCTTCATGCAACGCATCATAAATATTTTCAAAGTAACTAATATCACGCG‑TTAAGAAGCCATCGCGTTCCCCAGTGATTTTCATTAACTCAGCAAATGTTTTTAAACCTTCTCTATCAGATCGTTCTACTGTCGTACCTCGc               >  1:24995/1‑141 (MQ=255)                                                                                                                              tCATGCAACGCATCATAAATATTTTCAAAGTAACTAATATCACGCG‑TTAAGAAGCCATCGCGTTCCCCAGTGATTTTCATTAACTCAGCAAATGTTTTTAAACCTTCTCTATCAGATCGTTCTACTGTCGTACCTCGCttt            <  2:121565/141‑1 (MQ=255)                                                                                                                               cATGCAACGCATCATAAATATTTTCAAAGTAACTAATATCACGCG‑TTAAGAAGCCATCGCGTTCCCCAGTGATTTTCATTAACTCAGCAAATGTTTTTAAACCTTCTCTATCAGATCGTTCTACTGTCGTACCTCGCTTTa           <  2:238131/141‑1 (MQ=255)                                                                                                                               aaTGCAACGTATCATAAATATTTTCAAAGTAACTAATATCACGCG‑TTAAGAAGCCATCGCGTTCCCCAGTGATTTTCATTAACTCAGCAAATGTTTTTAAACCTTCTCTATCAGATCTTTCTACTGTCGTACCTCGCTTTa           <  1:159921/140‑1 (MQ=255)                                                                                                                                  gCAACGCATCATAAATATTTTCAAAGTAACTAATATCACGCG‑TTAAGAAGCCATCGCGTTCCCCAGTGATTTTCATTAACTCAGCAAATGTTTTTAAACCTTCTCTATCAGATGGTTCTACTGTCGTACCTCGCTTTAAAg        >  1:204758/1‑141 (MQ=255)                                                                                                                                        catcatAAATATTTTCAAAGTAACTAATATCACGCG‑TTAAGAAGCCATCGCGTTCCCCAGTGATTTTCATTAACTCAGCAAATGTTTTTAAACCTTCTCTATCAGATCGTTCTACTGTCGTACCTCGCTTTAAAGCCAAgc  <  1:467562/141‑1 (MQ=255)                                                                                                                                             |                                                                                                                                         CAGATGTTTTCATCTTCTGCTGCCATTTAGCAATTTCGGCATGAAGTTCATTCAATTCTTGATTTACTTTCGCTATATTTTCTTTTGGATCCAACTTTACTAAAAATAGTTCAGCATCTCCATCTTCATGCAACGCATCATAAATATTTTCAAAGTAACTAATATCACGCG‑TTAAGAAGCCATCGCGTTCCCCAGTGATTTTCATTAACTCAGCAAATGTTTTTAAACCTTCTCTATCAGATCGTTCTACTGTCGTACCTCGCTTTAAAGCCAAGC  >  CP000730/2384274‑2384549

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑