| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 2,268,572 | A→T | 10.7% | intergenic (‑460/+78) | USA300HOU_2142 ← / ← USA300HOU_2143 | hypothetical protein/possible HAD superfamily hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 2,268,572 | 0 | A | T | 10.7% | 170.2 / 11.5 | 56 | intergenic (‑460/+78) | USA300HOU_2142/USA300HOU_2143 | hypothetical protein/possible HAD superfamily hydrolase |
| Reads supporting (aligned to +/- strand): ref base A (21/29); new base T (4/2); total (25/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.91e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01 | |||||||||||
AAACAAGAAATATTATTCAAAATCAATGAATTTCCTATCTTATTAGTTTTAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTACCTATTTTATTATTCAGCTAAAAAATGCTCTGCTAAATATTTTGCTGCGCCATCCTCTTCATTCGTGTAT > CP000730/2268432‑2268710 | aaaCAAGAAATATTATTCAAAATCAATGAATTTCCTATCTTATTAGTTTTAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTaa < 1:540593/141‑1 (MQ=255)aaaCAAGAAATATTATTCAAAATCAATGAATTTCCTATCTTATTAGTTTTAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTaa < 1:540476/141‑1 (MQ=255) aaCAAGAAATATTATTCAAAATCAATGAATTTCCTATCTTATTAGTTTTAACAATATTTATTCTCATAGCGTTTGTCCAATTACATCTAGATGATTCAACTATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAAt < 1:78438/141‑1 (MQ=255) cAAGAAATATTATTCANAATCAATGAATTTCCTATCTTATTAGTTTTAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAg > 1:433344/1‑141 (MQ=255) aaGAAATATTATTCAAAATCAATGAATTTCCTATCTTATTAGTTTTAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGa < 1:539667/141‑1 (MQ=255) aaGAAATATTATTCAAAATCAATGAATTTCCTATCTTATTAGTTTTAAAAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGa < 1:468670/141‑1 (MQ=255) tattatTCAAAATCAATGAATTTCCTATCTTATTAGTTTTAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGTAAAGTTATTTTCTATTAGAAAAtg > 1:5080/1‑140 (MQ=39) ttattCAAAATCAATGAATTTCGTATCTTATTAGTTTTAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACt < 2:427650/141‑1 (MQ=255) aaaaTCAATGAATTTCCTATCTTATTAG‑‑TTAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGTAAAGTTATTTTCTATTAGAAAAtgtcttactgct < 2:225651/141‑12 (MQ=25) aaTCAATGAATTTCCTATCTTATTAGTTTTAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGTAAAGTTATTTTCTATTAGAAAAtgtcttactgct < 1:568568/141‑12 (MQ=37) aTCAATGAATTTCCTATCTTATTAGTTTTAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGctc < 2:206829/141‑1 (MQ=255) cAATGAATTTCCTATCTTATTAGTTTTAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑tt < 1:376043/141‑1 (MQ=255) cAATGAATTTCCTATCTTATTAGTTTTAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑tt < 2:409266/141‑1 (MQ=255) aaTGAATTTCCTATCTTATTAGTTTTAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑ttt > 1:155553/1‑141 (MQ=255) aTGAATTTCCTATCTTATTAGTTTTAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTg > 1:429684/1‑141 (MQ=255) gAATTTCCTATCTTATTAGTTTTAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTa > 2:560948/1‑141 (MQ=255) aTTTCCTATCTTATTAGTTTTAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACa < 2:508632/141‑1 (MQ=255) tCCTATCTTATTAGTTTTAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTa < 2:9803/141‑1 (MQ=255) ccTATCTTATTAGTTTTAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTTATAGAAAATAACTTTACTGCTC‑TTTGTTCATTAc > 2:117299/1‑141 (MQ=255) tATCTTATTAGTTTTAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACtc < 2:439368/141‑1 (MQ=255) cTTATTAGTTTTAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACtcatc < 1:329794/141‑1 (MQ=255) ttattaGTTTTAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACtcatca < 2:423608/141‑1 (MQ=255) ttaGTTTTAACAATAGTTATTCTCATAGAGCTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACt > 2:776905/1‑141 (MQ=255) taGTTTTAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACTa > 2:311468/1‑141 (MQ=255) ttttAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACTATTg < 2:462699/141‑1 (MQ=255) tAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACTATTGtaa < 1:358497/141‑1 (MQ=255) tAACAACATTTAGTCTAATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACTATTGtaa < 1:326653/141‑1 (MQ=255) tatttattCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACTATTGtaaattaaa > 1:392870/1‑141 (MQ=255) atttattCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACTATTGtaaattaaat < 2:691108/141‑1 (MQ=255) attCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACTATTGTAAATtaaataata < 2:582525/141‑1 (MQ=255) ttCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACTATTGTAAATtaaataataa > 1:261526/1‑141 (MQ=255) tctcATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACTATTGTAAATtaaataataaa < 2:761954/141‑1 (MQ=255) tcATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACTATTGTAAATtaaataataaata > 1:273861/1‑141 (MQ=255) aTAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAAc < 1:344046/141‑1 (MQ=255) tAGAGTTTGTCCACTTAAATATAGATGATTCAAATATATATAAGCACACAAAAGAGCAGT‑AAGACATTTTCTAATAAAAAATAACTTTACCGCTC‑TTTGTACATTCTGCAGCAGCTATGGTAAATTAAATAATAAATAACt > 2:13130/1‑141 (MQ=255) tAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACt > 2:424685/1‑141 (MQ=255) gTTTGTCCAATTAAATATAGATGATTAAAATAATAATAAAAAAAAAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTACCt < 2:354426/141‑1 (MQ=255) ttGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTACCTAt > 1:620458/1‑141 (MQ=255) tCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTACCTAtttt < 2:484539/141‑1 (MQ=255) tCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTACCTAtttt > 2:107796/1‑141 (MQ=255) tataGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTACCTATTTTATTATTCAGc < 1:288844/141‑1 (MQ=255) taGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTACCTATTTTATTATTCAGCTa < 2:157320/141‑1 (MQ=255) tatTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTACCTATTTTATTATTCAGCTAAAAAATGCTCTGc < 1:757402/141‑1 (MQ=255) taAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTACCTATTTTATTATTCAGCTAAAAAATGCTCTGCTAAata > 2:263706/1‑141 (MQ=255) taAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTACCTATTTTATTATTCAGCTAAAAAATGCTCTGCTAAata > 1:27908/1‑141 (MQ=255) acaaaGAGCAGTAAAGTTATTTTCTATTAGAAAATGTC‑TTACTGCTC‑TTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTACCTATTTTATTATTCAGCTAAAAAATGCTCTGCTAAATATTTtgctg > 1:594640/3‑141 (MQ=25) aaGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTTCCTATTTTATTATTCAGCTAAAAAATGCTCTGCTAAATATTTTGCTgcgc < 2:392364/141‑1 (MQ=255) agagCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTACCTATTTTATTATTCAGCTAAAAAATGCTCTGCTAAATATTTTGCTGCGcc > 2:87193/1‑141 (MQ=255) agCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTACCTATTTTATTATTCAGCTAAAAAATGCTCTGCTCAATATGTTGCTGCGCCAt > 1:241856/1‑141 (MQ=255) ccgT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTACCTATTTTATTATTCAGCTAAAAAATGCTCTGCTAAATATTTTGCTGCGCCATcc < 1:559072/139‑1 (MQ=255) cAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAGCCGCCTATTTTATTATTCAGCTAAAAAATGCTCTGCTAAATATTTTGCTGCGCCATcc < 1:280311/141‑1 (MQ=255) cAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTACCTATTTTATTATTCAGCTAAAAAATGCTCTGCTAAATATTTTGCTGCGCCATcc < 2:684422/141‑1 (MQ=255) gT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTACCTATTTTATTATTCAGCTAAAAAATGCTCTGCTAAATATTTTGCTGCGCCATCctc > 2:430182/1‑141 (MQ=255) aagttaTTTTCTATTAGAAAATGTC‑TTACTGCTC‑TTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTACCTATTTTATTATTCAGCTAAAAAATGCTCTGCTAAATATTTTGCTGCGCCATCCTCTTc > 2:23903/6‑141 (MQ=38) agttaTTTTCTATTAGAAAATGTC‑TTACTGCTCTTTTGTACATTACTCATCAACTATTGTAAAGTAGATAATTAAGAACTAGCAATTGTATGATTCAGCTAAAAAATGCTCTGCTAAATATTTTGCTGCGCCAGCCTCTTc > 1:664556/5‑141 (MQ=21) ttCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTACCTATTTTATTATTCAGCTAAAAAATGCTCTGCTAAATATTTTGCTGCGCCATCCTCTTCATTCgtg < 2:380704/141‑1 (MQ=255) ttCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTACCTATTTTATTATTCAGCTAAAAAATGCTCTGCTAAATATTTTGCTGCGCCATCCTCTTCATTCgtg > 2:649921/1‑141 (MQ=255) tAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTACCTATTTTATTATTCAGCTAAAAAATGCTCTGCTAAATATTTTGCTGCGCCATCCTCTTCATTCGTGTAt > 2:666131/1‑141 (MQ=255) | AAACAAGAAATATTATTCAAAATCAATGAATTTCCTATCTTATTAGTTTTAACAATATTTATTCTCATAGAGTTTGTCCAATTAAATATAGATGATTCAAATATTTATAAGCATACAAAAGAGCAGT‑AAGACATTTTCTAATAGAAAATAACTTTACTGCTC‑TTTGTACATTACTCATCAACTATTGTAAATTAAATAATAAATAACTACCTATTTTATTATTCAGCTAAAAAATGCTCTGCTAAATATTTTGCTGCGCCATCCTCTTCATTCGTGTAT > CP000730/2268432‑2268710 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |