Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,296,304	A→C	9.8%	intergenic (+510/+132)	gltP → / ← yjcO	glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_000913	4,296,304	0	A→C	9.8%	13.7 ‑0.4 ‑0.3	70	intergenic (+510/+132)	gltP/yjcO	glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  new base (2/6):  ref base (24/37):  total (26/44)
Fisher's exact test strand distribution p-value = 7.01e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.20e-01

TTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGC‑GTT‑T‑‑A‑‑C‑‑CGC‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAA  >  NC_000913/4296019‑4296601                                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                                                    ttGGAGGTCGGATTATGCGTTTACCCCGACTCCGCTAGCCACCCCGGAGGCTACGCTGTCCGCGTCTGATCAGGCCTAGGCCAGACAGCGCAATAGCCTGATTTAGCGGGATTTTGTAGGTCGGATAAGGCGTGTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc                                                                                                                                                                                                                                                                                                      <  2:170302/301‑1 (MQ=11)  tgttgggcagaaacggCGTTGACGCCGTCTCCGACATCACAGCCGTATGAGGCGCTTACCAGGTCGTATCAGGCCTACGCCAGCAAGAGCAATAGCCTGATTTAGCGTGATTTTTTAGGGCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGAGACGCTTAAGGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGcc                                                                                                                                                                                                                                                                                                     <  2:301571/287‑1 (MQ=11)        tgggATAAGGTGTTTACGCCGCATCCGACAGCAACGCTAGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatct                                                                                                                                                                                                                                                                                                <  1:283563/298‑4 (MQ=255)        gctgATGAGGCTTTGACTCCGCATCCGACATTAACGCCTGTTGCGACGCGGTCCTCGTCTTATCAGGCACACGCCACACAGCGCAATCGCCTGATTTAGAGTGAGTTTGTAGGTCGGATAAGTCTTTTATGCCGCATCCGACATCAACTCCTTATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGAGTGATTTCTTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATTCGACGCTTGCCGCGTCTTATCAGGCCTatct                                                                                                                                                                                                                                                                                                <  1:96049/297‑4 (MQ=11)        gcGGATAGGGTGTTTACTCCGCAGGCGAGATGAGCGCCGGAGGCGCCGCTGGCCGCGTCTTATCAGGGCTACGCCAGACAGCGCAATAGGCGGATTTAGCGTGATTTTGTTGGTCGGATAAGGCGTTGATGCCGCATCCGACATCAACGCGTGATGCGACGCTGAACGCGTCGTCTCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTatct                                                                                                                                                                                                                                                                                                <  1:986863/299‑4 (MQ=11)                ggCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGCTCGGATACGGCGTTTACGCCGCATCCGACATCCATCCCTGCTGCGTCGCTTGCCGCGTCTTTTCTGGCCTatcttcaccg                                                                                                                                                                                                                                                                                          >  2:10250/1‑289 (MQ=11)                                                                                                               aTTTTGTAGGTCTTATAAGTCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGACAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGC‑GTT‑T‑‑ATGC‑‑CGC‑ATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATc                                                                                                                                                                                 <  1:576807/300‑1 (MQ=2)                                                                                                                             acaaggcgtctcgcctgCACCCGGCATCACCGCCGATTGCGACCCTTTCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTTATTTTGTAGGTCGTATAAGGCGTTGATGCCGCATCCGACATCAACGCCGGATGCGACGCTTAACTCGTCTTATC‑GGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGC‑GTT‑T‑‑ACGC‑‑CGC‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAAcc                                                                                                                                                                  <  1:65807/284‑1 (MQ=11)                                                                                                                             aTAAGGCTTTTACTCCGCCTTCGCAGTCAACGCCTGATGCTACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTTATTTTGTAGGTCTGATAAGGCTTTTATGCCGGATCCGACATCAACGTCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGC‑GTT‑T‑‑A‑‑CGGCGC‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAAcc                                                                                                                                                                  <  1:567458/301‑1 (MQ=11)                                                                                                                             aTAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGC‑GTT‑T‑‑ACGC‑‑CGC‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAAcc                                                                                                                                                                  <  1:702641/301‑1 (MQ=2)                                                                                                                              tacggcgttgccgCCGCATCCTCCACCACCGCCTGTTTCGCCCCTTGCCGCGTCTTATCAGGCCTACGCCAGCCAGCGCAATGGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGGTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGC‑GTT‑T‑‑ACGC‑‑CGC‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCg                                                                                                                                                                 <  1:18996/289‑1 (MQ=11)                                                                                                                                 ggCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCCGGCCTACGCCCGACCGCGCAATAGCCTGCTTTAGCGTG‑ATTTTGTCGGTCGGATAAGGC‑GTT‑T‑‑ACGC‑‑CCC‑CTCCGCCATCAATCCCTGATGCGACGCTTGCCGCCTCTTTTCAGTCCTATCTTAACCGTTg                                                                                                                                                              >  2:325287/1‑301 (MQ=11)                                                                                                                                         tGCCGCACCCGACAACAACTCCCAGTGCGATGCTTAACGGGTCTTCTTAGGCTGACGGCAGACAGCGCAAGAGCCTGATTGAGCGTGATTTGGGTGTTCGGATAAGGCGTTTAGTACGCTTCCGACACCACCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGC‑GTT‑T‑‑ACGC‑‑CGC‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTCAAttt                                                                                                                                                      <  1:727353/301‑1 (MQ=11)                                                                                                                                           ccGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGACAGACAGCGCAATCGCCTGATTTAGCGTGATTTTGTAGTTCGGATAAGACGTTTATGCCGCAGCAGACATTAACGCCTGATGCGCCGATTAACGCGTCTTATCAGGCCTGCGCCAGACAGCGCAATCGCCTTCCTGATCGTC‑ACTTTGTGGTTCGGATAAGGC‑GTT‑T‑‑A‑‑CG‑AACAATCCAACATCCATGCTTGATGCACAGCTTGCCCCGTCTTATAACCCGTCTCTTACCCGTTGCTTACttctt                                                                                                                                                    >  2:686958/1‑301 (MQ=2)                                                                                                                                                     cATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCTGATCAGGC‑GTT‑T‑‑ACGC‑‑CGC‑ATCAGACATCAATGCCTGATGCGCCGCTTTCCGCGCCTTATCCGGCCTCTCTTAACCGTTGGTTAATTTTTCAAACTCtt                                                                                                                                           >  2:35968/1‑300 (MQ=11)                                                                                                                                                      aTCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCACTAGCCTGATTTAGCGTG‑CTTTTGTAGGTCGGCTAATGC‑GTT‑T‑‑ACGC‑‑CGC‑ATCCGACATCAATTCCTGCTGCGACGCTTGCCGCGTCTTCTCGTGCCTCTGTTACCCGTTGATTCATTTTTCACACTCTTc                                                                                                                                          >  1:803739/1‑300 (MQ=11)                                                                                                                                                          tgggccgtgtcatcgccttccgcgTCTTAGAAGGGCTACAGCAAACAGCGCAAGCTCCTGATTTAGCGTGATTGTGGAGGTCGGATAAGGCATTTATGCCCCATCCGAAATCAAGACCTGATGCAGCGCTTAGCGCGTCTTATCAGGCATACGCCAGACAGCGCAATAACCGGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGC‑GTT‑T‑‑ACGC‑‑CGC‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc                                                                                                                                     <  2:917813/281‑1 (MQ=14)                                                                                                                                                                 aggCGAAGCGTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGACTGATTTAGCGTGATTTTGTAGGTCGGATAAGGTGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAAGAGCCTGAGTTAGCGTG‑ATTTTGTAGGTCGGATAAGGC‑GTT‑T‑‑ACGC‑‑CGC‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCg                                                                                                                                                                 <  1:870390/264‑1 (MQ=11)                                                                                                                                                                 aTGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGC‑GTT‑T‑‑ACGC‑‑CGC‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGTTAt                                                                                                                               >  1:184299/1‑300 (MQ=255)                                                                                                                                                                 aTGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCATGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGC‑GTT‑T‑‑ACGC‑‑CGC‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTCTCTTCACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTAt                                                                                                                               >  1:532607/1‑300 (MQ=255)                                                                                                                                                                      tgggttgacgtagcgtaggagccatACGCCAGAGTGCGGAATAGCGTTGGTTAGGGGGATGTTGTGTGGCGGAAAAGGCGTGTAGGCCGCGTCCGAAAACAAGGTCTGAGGCGGCGCTTAATGCGTGTTATGAGGCCTACGCCAGACAGCGCAATATCCTGATTTAGCGTG‑ATATTGTAGGTTGGATAAGGC‑GTA‑T‑‑ACGC‑‑CGC‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGACTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGCATCAAAc                                                                                                                          <  2:672921/276‑1 (MQ=255)                                                                                                                                                                      gctgtttccgcttattgtgagtatacagCCAGGAAGCTCTATAGACTGATTGAGCGTGATTTTGTAGGTCGGCTAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTAGCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGC‑GTT‑T‑‑ACGC‑‑CGC‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAAc                                                                                                                          <  2:467105/273‑1 (MQ=14)                                                                                                                                                                      acggttgtcggttCTTATCATTCATACGCCATACAGCGCAATAGCCTGATTTAGCGTTATTTTGTAGGTCGGATAAGGCGTTTATTCCGCATCCGACATCTACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCTTG‑ATTTTGTAGGTCGGATAAGGC‑GTT‑T‑‑ACGC‑‑CGC‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAAc                                                                                                                          <  1:317683/288‑1 (MQ=14)                                                                                                                                                                      aCGCTTGCCGCGTCTGATCAGGCCTACGCCAGCCAGCGCAATAGCCTGATTTCGCGTGATTTTGTAGGGCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGC‑GTT‑T‑‑ACGC‑‑CGC‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAAcc                                                                                                                         <  1:211845/301‑1 (MQ=255)                                                                                                                                                                               tgtgtttATGAGGCGGACGACAGAAAGCGCAATAGCGTGATGTTGCGTTATTTTGTAGGTCGGATAAGGCGTTTAGGCGGCATCCGACACCAACGCCTGATGCGACGCTTAACTGGTCTGATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCAGATAAGGC‑GTT‑T‑‑ACGC‑‑CGC‑AGCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATAc                                                                                                                <  2:675663/296‑1 (MQ=17)                                                                                                                                                                               tcgtcgtacgcgtgcgaggCCAGACGGCGGAATAGCAGTAGTTAGCGTGATTTGGTAGGTCGGATAAGGCGGTGATGCCGCATCCGACATCAACGCCGGATGCGACGGTTAACGCGTCTTAGCAGGCCTACGCCAGACAGCGCAATAGCCGTATTTAGCGTG‑ATTATGTAGGTCGGATAATGC‑GTT‑T‑‑ACTC‑‑CGC‑ATCCGACATCAAGGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCAGTATCAAACCCTTCCATAc                                                                                                                <  1:48999/283‑1 (MQ=17)                                                                                                                                                                                tggtgtATCAGGCCTACGCCAGACAGCGCAATAGCCGGATGGAGCGTGATTTTGTAGGTCGGATAAGGCGTTTAGGCCGAAACGGGAATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGC‑GTT‑T‑‑ACGC‑‑CGC‑ATCCGACATCAATGCCTGATGCGACGCTGGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATAc                                                                                                                <  2:93915/295‑1 (MQ=17)                                                                                                                                                                                cgTCTTAGCAGGCCTACGCCAGAAAGCGCAATGGCCTGATTTAGCGTGATTTTGTAGGGCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGC‑GTT‑T‑‑ACGC‑‑CGC‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATAc                                                                                                                <  1:869715/300‑1 (MQ=255)                                                                                                                                                                                cgTCTTAGCAGGCCTAAGCCAGAAAGAGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGC‑GTT‑T‑‑ACGC‑‑CGC‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATAc                                                                                                                <  1:35969/300‑1 (MQ=255)                                                                                                                                                                                cgTCTTAGACGGCCTACGCCAACCCGCGAAATAGCCTGATTTAGCGTGATTTGGTAGGTCGGATAAGGCGTTTATGCCGCATCGGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAAGAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGC‑GTT‑T‑‑ACGC‑‑CGC‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATAc                                                                                                                <  1:1479/300‑1 (MQ=17)                                                                                                                                                                                cgTCTGATGAGGCCTACGCCAGAAAGCGAAATAGACTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGC‑GTT‑T‑‑ACGC‑‑CGC‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATAc                                                                                                                <  1:626932/300‑1 (MQ=255)                                                                                                                                                                                          ggCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGCCAGCGCGATAGACTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGC‑GTT‑T‑‑ACGC‑‑CGC‑ATCCGACATCAATGCCTTATGCGACGCTTGCCGCGTCTTCTCGGCCCTCTCTTCCCCTTTGTTTAATTTTTCAAACTCTTCACACCCGGTCTCAAACCCtttcccttatacccatctt                                                                                                     >  2:810734/1‑284 (MQ=14)                                                                                                                                                                                          ggCATAAGCCAGACAGAGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCTGTTAGGCCTCAGCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGC‑GTT‑T‑‑ACGC‑‑CGC‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAAcc                                                                                                                         <  1:810760/281‑1 (MQ=14)                                                                                                                                                                                           gCCTACTGCAGAAAGCGCAATAGCCTTATTGTGGGGGATTTTGTAGGTCGGATAATGCGTTTATGCCGCACCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGC‑GTT‑T‑‑ACGC‑‑CGC‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGtt                                                                                                      <  2:465103/299‑1 (MQ=17)                                                                                                                                                                                              tACGCTAGACACCGAAATAGCCTTATTTAGCGTGAGTGTGTAGTTGTGATAATGCTTTTAGGCCGCATCCGACATCAACGACTGATGCGACGCTTAACGCTTCTGATCAGGCCTACACCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGTATAAGGC‑GTT‑T‑‑ACGC‑‑CGC‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTGCCATACAGCTCAGGTTCAAcc                                                                                                 <  1:670531/301‑1 (MQ=17)                                                                                                                                                                                              tACGCCAGACCGAGCAATAGCCTGATTTAGCGTTATTGTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGC‑GTT‑T‑‑ACGC‑‑CGC‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAAcc                                                                                                 <  1:713770/301‑1 (MQ=255)                                                                                                                                                                                               aCGCCAGACAGCGCAATAGCCTGCTTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCACTAGCCTGATTTAGCGTC‑ATTTTGTAGTTCGGATAAGGC‑GTT‑TCCG‑‑C‑‑CGC‑AGCCGACAGCATTTCCTGATGCGACCTTTGCCGCGTCTTATCAGCCCTCCCTTACACTTTGGTTAATTTTTCCACCTCTTCACCCCCGCTCACCAACCCTTCCATTCCTCTCAGGTTCACCCa                                                                                                >  2:903925/1‑301 (MQ=11)                                                                                                                                                                                               aCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGCTTTAGCGTG‑ATTTTGTAGGTCGGATAAGGC‑GTT‑T‑‑ACGC‑‑CGC‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCCGGCCTATCTTAACCGTTGGTTACTTTTTCCACCTCTTCACACCCGGTCTCAAACCCTTCCATACCACTCAGCTTccccc                                                                                                 >  1:853935/1‑296 (MQ=14)                                                                                                                                                                                               aCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATACGCC‑GTT‑TCGC‑‑C‑‑CGC‑TTCCAACACCAATGCCTGATGCACCGCTTCCCGCTTCTTATCAGCCCTATCTTACCGGTTGGTAAATTTTTCAAACTTTCCCCCCCCGGTGTCAACCCCTCCCATCCACCCCAGGtcacacca                                                                                                >  1:136375/1‑294 (MQ=14)                                                                                                                                                                                               aCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGC‑GAT‑T‑‑ACGC‑‑CGC‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCATACTCTTCACACCCGGTGTCAAACCATTCCATCCAGCTCAGGTTCCACCa                                                                                                >  1:138140/1‑301 (MQ=255)                                                                                                                                                                                                 gCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTCTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTCCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGCTTTAACGTG‑ATTCTGTATGTCGGACAAGGC‑GTT‑T‑‑ACGC‑‑CGC‑ATCCGACATCAATTCCTGCTGCTCCGCTTGCAGCGTCGTATCCGGCCTATGTTAACCGTTTGTTCATTTTTCAAACCATTCACACCCGGTCCCCAACCCCTCCAtcagccacagttccccccata                                                                                              >  2:839109/1‑281 (MQ=11)                                                                                                                                                                                                        cgcgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGAGTGTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGC‑GTT‑T‑‑ACGC‑‑CGC‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAgcgc                                                                                       <  1:626411/300‑1 (MQ=255)                                                                                                                                                                                                         gcgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGCTAACGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTCGCGTG‑ATTTTGTAGGTCGGATAAGGC‑GTT‑TCCG‑‑C‑‑CGC‑ATCCGACATCACTGCCTGATGCGCCGCTTTCCGCGTCTTATCAGTCCTATCTTAACCGTTGGTTACTTTTTCAAACTCTTCACACCCGATATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAgctg                                                                                       >  1:212155/1‑298 (MQ=17)                                                                                                                                                                                                            cAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGC‑GTT‑T‑‑ACGC‑‑CGC‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTCTCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCttt                                                                                   >  1:81666/1‑301 (MQ=255)                                                                                                                                                                                                               tAGCCTGATTTAGCGTGGTTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGC‑GTT‑T‑‑ACGC‑‑CGC‑ATCCGACATCAATGCCTGATGCGACGCTTGGCGCGTCTTATCAGGCCTATCTTAACCGTGGGTTCATTTTTCAAACTCTTCACACACGCTATCACACACTGCGATCCAGCTAAGGTTCAAGCAGTGCAGCTCCTTTTg                                                                                 >  1:885083/1‑300 (MQ=14)                                                                                                                                                                                                                 ggcagcgtaagaggggtttaagggacaggATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGC‑GTT‑T‑‑ACGC‑‑CGC‑AGCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTACCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCt                                                                               <  2:300002/273‑1 (MQ=18)                                                                                                                                                                                                                 gCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACTCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGC‑GTT‑T‑‑ACGC‑‑CGC‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTGTCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGGtt                                                                              >  1:631312/1‑301 (MQ=255)                                                                                                                                                                                                                     taTTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGCACTCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGC‑GTT‑T‑‑ACGC‑‑CGC‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGttc                                                                          <  1:38429/300‑1 (MQ=255)                                                                                                                                                                                                                     gATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGC‑GTT‑T‑‑ACGC‑‑CGC‑ATCCGACATCCATGCCTGATGCGCCGCTTGCCGCGTCTTATCAGGCCTATCTTTACCGTTGGTTACTTTTTCAACCTCTTCACCACCCGCATCCCACCCTTCCATACCCATCACCTTCAACCCATCCACCCCCTTTTTCTTGtt                                                                           >  2:981029/1‑300 (MQ=14)                                                                                                                                                                                                                      aTTGAGCGTTATGTTGTAGGTCGTATAAGGCTTTGATGCCGCATCCGAAATCAACGGCTGATGCGACGCTTAACGCGTCTTAGCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTATCGTG‑ATTTTGTAGGTCGGATAAGGC‑GTT‑T‑‑ACGC‑‑CGC‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGttc                                                                          <  1:872673/300‑1 (MQ=18)                                                                                                                                                                                                                                aTTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCCGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGCTAAGGC‑GTT‑T‑‑ACGC‑‑CGC‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTCATTTTTCAAACTCTTCACACCCGGTACCCAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGACCCTTTTGCTTGTTCTTCTCGATaaa                                                               >  2:563201/1‑301 (MQ=255)                                                                                                                                                                                                                                  tttGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGC‑GTT‑T‑‑ACGC‑‑CGC‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCACACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATaaaa                                                              >  1:764983/1‑300 (MQ=255)                                                                                                                                                                                                                                          tCGGATAAGGCGGTTAGGCCGCATCCGAAATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGC‑GTT‑T‑‑ACGC‑‑CGC‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCttcttc                                                      <  1:475141/300‑1 (MQ=255)                                                                                                                                                                                                                                            ggaaaaggaggtgaggccgaccccGACAGCACCGCCTGATGCGACGCGTAACGCGTCGTATCAGGCCTACGCCAGAAAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGC‑GTT‑T‑‑ACGC‑‑CGC‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCAc                                                    <  2:976867/278‑1 (MQ=21)                                                                                                                                                                                                                                            ggATAAGGCGTTTACGCCGCATCCGACATCAACGCCGGGTGCGACGCTTAACGCGTTTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGC‑GTT‑T‑‑ACGC‑‑CGC‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCAc                                                    <  2:214558/300‑1 (MQ=255)                                                                                                                                                                                                                                             gATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGC‑GTT‑T‑‑ACGC‑‑CGC‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTACCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCt                                                                               >  1:300008/1‑272 (MQ=255)                                                                                                                                                                                                                                              aTAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTGAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGC‑GTT‑T‑‑ACGC‑‑CGC‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCg                                                  <  1:790361/300‑1 (MQ=255)                                                                                                                                                                                                                                                  ggCGTTTATGCCGCCTCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGC‑GTT‑T‑‑ACGC‑‑CGC‑ATCCGACATCAATGCCTGATGCGACCCTTGCCGCGTCTTATAAGGCCTCTCTTAACCGTTGGTTACTTTTTCAAACTCTTCACACACGGTATCACACCCTTCCATACAGCTCAGTTTCCACCACTGACCCGCCTTTTCCTTGTTCTTCTCTATAAAACCTTCTTCACCTTTTaa                                             >  2:597705/1‑301 (MQ=18)                                                                                                                                                                                                                                                  ggCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGC‑GTT‑T‑‑ACGC‑‑CGC‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTTATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCCGTGCAGCGCCTTTTCCTTGTTCTTCTCGATAAAGCCTTCTTTCCCGTTTaa                                             >  2:582823/1‑301 (MQ=255)                                                                                                                                                                                                                                                  ggCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGC‑GTT‑T‑‑ACGC‑‑CGC‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCCAACTCTTCACACCCGGTATCAAACCCTTCCATCCACCTCAGGTTCAACCAGTGCCGCGCCTTTTTCTTGTTCTTCTCGATACAAACTTCTTCACCGtttca                                             >  2:229961/1‑299 (MQ=21)                                                                                                                                                                                                                                                    cGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCATACATCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGC‑GTT‑T‑‑ACGC‑‑CGC‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAg                                            <  1:230942/300‑1 (MQ=255)                                                                                                                                                                                                                                                    cGTTTATGCCGCATCCGACATCAACGCCTGATCCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATGTTGTAGGTCGGATAAGGC‑GTT‑T‑‑ACGC‑‑CGC‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAg                                            <  1:563211/300‑1 (MQ=255)                                                                                                                                                                                                                                                           cacGCCTCCGACAACATCGCTTGATGCGAGGCTGTACGTGTTTGATCAGGCGTACGCCAGACCGCGCAATGGCCTGAGTTGGCGTG‑ATTTGGTAGGTCGGATAAGGC‑GTT‑T‑‑ACGC‑‑CGC‑ATCCGATATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCGGTGCAGAGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAAcatcat                                    <  2:502632/299‑1 (MQ=25)                                                                                                                                                                                                                                                              ccagcccaaatgaaCGCCCGATGGCAAGCTTAAAGGGGTTTATGAGGCCTACGCCCGACCGCGCAATAGCCTGATTTAGCGGT‑AGTTTGGTGGTCGGATAAGGC‑GTT‑T‑‑ACGC‑‑CGC‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTcc                                 <  2:136372/289‑1 (MQ=25)                                                                                                                                                                                                                                                                        tCAACGCTTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGC‑GTT‑T‑‑ACGC‑‑CGC‑ATCCGACCTCAATGCCTGATGCGACGCTTGCCGCGCCTTATCACGCCTATCTTAACCGTTGGTTAATTTTTCAACCTCTTCACACCCGGTATCAAACCCTTCCATACAGCGCAGGTTCACCCAGTGCAGCCCCTTTTGCTTGTCCTTCTCCATCAAACCTTCTTCACCCTTCAAGCACACCCTTCCACCCCattc                        >  2:787362/1‑297 (MQ=21)                                                                                                                                                                                                                                                                             ccctgagtccaCGCTTAACGCGTCTTATCAGGACGACGCCAGACAGCTCAATAGCCTGATTTAGCGTG‑ATTGTGTAGGTCGGATAAGGC‑GTT‑T‑‑ACGC‑‑CGC‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGa                  <  1:321053/291‑1 (MQ=37)                                                                                                                                                                                                                                                                             cccTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGC‑GTT‑T‑‑ACGC‑‑CGC‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGa                  <  2:419896/300‑1 (MQ=255)                                                                                                                                                                                                                                                                               tggaggCGACGCTTAACGATGCTTATCAGGCCTACGACATAACTCGCAATAGTCTGATTTAG‑GTGTATTTGTTAGGTGGGTTAAGGCTGTTAC‑‑A‑‑C‑‑CGA‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCGTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGaa                 <  2:147859/295‑1 (MQ=37)                                                                                                                                                                                                                                                                                       aCGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGC‑GTT‑T‑‑ACGC‑‑CGC‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGtt        >  1:585378/1‑301 (MQ=255)                                                                                                                                                                                                                                                                                         gCTTAACGCGTCTGATCAGGCATACGCCAGACAGCGCAATAGCCTGATGTAGCGTG‑ATGTTGTAGGTCGGATAAGGC‑GTT‑T‑‑ACGC‑‑CGC‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAAcc                                                            <  1:896254/247‑1 (MQ=255)                                                                                                                                                                                                                                                                                             aaCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGC‑GTT‑T‑‑ACGC‑‑CGC‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGaa  <  2:184295/301‑1 (MQ=255)                                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                                                    TTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTG‑ATTTTGTAGGTCGGATAAGGC‑GTT‑T‑‑A‑‑C‑‑CGC‑ATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAA  >  NC_000913/4296019‑4296601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.