Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,815,809	+A	13.5%	intergenic (‑41/+25)	pyrE ← / ← rph	orotate phosphoribosyltransferase/ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_000913	3,815,809	0	.→A	13.5%	13.2 0.0 ‑0.0	44	intergenic (‑41/+25)	pyrE/rph	orotate phosphoribosyltransferase/ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH
Reads supporting (aligned to +/- strand):  new base (2/2):  ref base (21/17):  total (25/19)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.95e-01

GTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACA‑AGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCTTTCATCGGATTGGTTTTCAG  >  NC_000913/3815533‑3816102                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             gtggCAATCGGGATCCCTTTGTAAGCAGGGCACAACAGAAGATCGAACTCAATGCCGGAATCCACCAACGGTTCAGCGTAAAAACGGCCTAACAGGGCCAGATCGCGACCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTaaaaa                                                                                                                                                                                                                                                                                <  1:344064/300‑1 (MQ=255)                  tttGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCCAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACCAAAAAAAGAC‑ACTCATCAGTACCCTTAAAAATCAGTTTGCCAGcgccc                                                                                                                                                                                                                                                               >  1:164590/1‑299 (MQ=255)                  tttGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATTCCTTCGCTCCTCTTCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAACAATCAGTTTGCCaaccccc                                                                                                                                                                                                                                                               >  1:34601/1‑294 (MQ=255)                          cAGGGCCAAACAGCAGATCGAACTACAGGCCGGAACCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATTGCGGCCGGTATTAAACAGTCTGGCGTTGAAGAAATAGGGGCTTTTGCGCTCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCACATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAGAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTg                                                                                                                                                                                                                                                        <  2:164591/299‑1 (MQ=255)                           aGGGCCAAAGAGCAGCTCGAACTCAATGCCGGAATGCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGAGCGCGCCCGGTATGAAACAGCCCGGCGTTGAAGAAATAGGGGCTGTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCg                                                                                                                                                                                                                                                      <  1:72637/300‑1 (MQ=255)                             tgtcaaacgtcaaaccaaaCTCATGGCCAGAATCCAAACACGCGCCAGCGTAAAATGGTCCTAAAATGTCCAGAGCGCGCCCGGTATTAAACATCCCGGCGTTGAAGAATTAGTGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCTCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGt                                                                                                                                                                                                                                                     <  2:112605/282‑1 (MQ=255)                                         gATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAAATTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTCAAATGgatt                                                                                                                                                                                                                                       >  2:309763/1‑301 (MQ=255)                                                               caccaACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCACACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTCCAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCCGTTTGCCAGCGCCTCCTTCTGCGTCGCTACAATGGATTCAATTCCCCCCGGGCCagccc                                                                                                                                                                                                                  >  1:192569/1‑297 (MQ=255)                                                                                     cGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATTTTACTTTTATACAGA‑AAAAAAAAAGCGACTCATCAGTCGCCTTACAAATCAGTTTGCACGCGCCGCCTCCTGCGTCGCTACAATGGATTCGATTCCCCTAGGGCCAGAG‑CAACACAGCAGAGTAGCTCTACAt                                                                                                                                                                                          >  2:139849/1‑301 (MQ=255)                                                                                               gTGCCAGATCGCGACCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAAAACCTGATTGCTAAGCGCAAATTCAATAAACTGGCGCTGCTATGGTTTCATGCCTTCGCTCCTCATCTTAATTTTCTACAGACAAAACAAA‑ACGACTCATCCGTCGCCTTGAAACTCAGTTTGCCAGCGCAGCCTACTACGACGCTAACATGGCTTTGATGACCATAGGGACAGCAACCACA‑AGCTGCGTAGCTGTTCATGGGATAACgg                                                                                                                                                                                >  2:150874/1‑301 (MQ=255)                                                                                                tGCCAGATCGCGCCCTGTATGAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACA‑AGATGAGTAGCTCTTCATGGGTGAACgg                                                                                                                                                                                <  2:34601/300‑1 (MQ=255)                                                                                                 gCCAGATCGCGCCCGGTATTCAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACACTGGATTCGCTTCCCCTCGGCCCCGAGCccaccacgttactcgctcttccttgttgacccca                                                                                                                                                                                >  1:362838/1‑270 (MQ=255)                                                                                                 gCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTGCTCATCTTACTTTTCTACAGACAAAAAACA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCACCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGCGCCCACA‑AGATGCGTAGCTCTTCACTGCTGAACgg                                                                                                                                                                                >  2:166413/1‑299 (MQ=255)                                                                                                                gtaaaaaaCAGCCCGGCTGTGAAGAAATAGGGTCTTGTGCGCCCGGATTTCAGCGTAACATCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACA‑AGATGAGTAGCTCTTCATGGGTGAAc                                                                                                                                                                                  <  2:5455/277‑1 (MQ=255)                                                                                                                gTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGCTTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTCCAGAC‑CAAACAACGCGACTCATCAGTCGCCTTAACAATCAGTTTGCCCGCGCCGCCTTCTGCGTCGCTACAATGGGTTCGCTTCCCCCCGGGCCAGCCCCCACC‑AGTTGTGTAGCTCTTCCTGGTTTCACGGCCCGCCTTCTCCCCTc                                                                                                                                                                >  1:239406/1‑300 (MQ=255)                                                                                                                gTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATTCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCCCGATCCAGACCCAAAC‑AGATGAATAGCTCTTCCTGGTCAAACGGCCCGCCTTCTCCCCTc                                                                                                                                                                >  1:405787/1‑300 (MQ=255)                                                                                                                gTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTCCAATGGATTCGCTTCCCCTCGCGCCAGCGCCACCA‑AACTGAGTAGCCCTTCATGGGTGaccctgtctcttatccacaa                                                                                                                                                                 >  1:5455/1‑280 (MQ=255)                                                                                                                gTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACACTGGATTCAATTCCCCCCGGGCCCGAGCCAACC‑ACATGAGACACCCTTCCTGGGTGAACGGCCAGCCTTCTCCCGTc                                                                                                                                                                >  1:129827/1‑300 (MQ=255)                                                                                                                          gCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTACTCAGTCGCCTTACAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTGGGGCCAGAGCAAACC‑AGCTGAGTAGCTCTTCATGGGTGACAGGACAGCCTTCTGCCGCACCCTGGACtt                                                                                                                                                      >  2:162077/1‑300 (MQ=255)                                                                                                                           cccGGCGTTGAAAAAATAGGGGCTTTTGCGCCCTGATTTCAGCGTAAACTCGCCAAACTTTAACCCCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACA‑AGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCAc                                                                                                                                                        <  1:139848/297‑1 (MQ=255)                                                                                                                                    gaagaaATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCCGCGCCAACA‑AGATGAGTAGCTCTTCCTGGGTGACCGGCTCGCCTTCTGCCGCCCCCTGCCCTTCCATGATgcg                                                                                                                                            >  1:37501/1‑300 (MQ=255)                                                                                                                                         aaTAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTCCAGACAAAAAACA‑GCGACTCATCAGTCGCCTTAAAAATAAGTTTGCCAGCGCCGCCTTCTCAGTCGCTACAATGGATTCGATTACCCTCTGCCCCTAGCCACCA‑CACTTAGTATCCCTTCTTGTGTGACATGCTCGCCTTCTTCCTTCCCATGCACTCCActgatcacccatc                                                                                                                                       >  2:388206/1‑292 (MQ=255)                                                                                                                                            tggggCTTTTGCGCCCGGATTTCAGCGTAAACCCTCCAAACGTTAACACCTGCTTGCTAAGCCCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCCTCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACA‑AGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTc                                                                                                                                   <  1:309755/300‑1 (MQ=255)                                                                                                                                               ggCTTTTGCGCACGGATTTCAGCGTAAACCCGCCAAACTGAAACACCTGCTTGCTAAGCCCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACA‑AGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCgg                                                                                                                                 <  1:166412/300‑1 (MQ=255)                                                                                                                                                       ctcctctaTTTCAGCCGCAACCCGCCCAATTTTTACGCCGTCTTGCCAACCGGACATTCATTACAATGGCTCTTATTTGGTTTCATGCCTGCGATCCACAGCGTACGTGCCTACCGTCAAAAAAAA‑GCGACTCTTCTGTCGCCTTAAAAATCAGTTTGCCAGCGCCTCCTTCGGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACA‑AGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACt                                                                                                                         <  2:269440/293‑1 (MQ=255)                                                                                                                                                               tttCAGCGTAAACTCCCCAAACTCTAACCACCGATTACCAATCGCAAATTCATAAAACTGAAGCTGATAGTGTTTCATGCCTTCCTTCCTCATCTTATTTTTCTACAGACAAAAAAAA‑TCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACA‑AGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATg                                                                                                                <  1:150873/301‑1 (MQ=255)                                                                                                                                                                           cTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACA‑AGATGAGTAGCTCTTCATggg                                                                                                                                                                                       >  2:92786/1‑218 (MQ=255)                                                                                                                                                                                    cTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTc                                                                                                                                                                                                                            >  1:113752/1‑173 (MQ=255)                                                                                                                                                                                    cTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTc                                                                                                                                                                                                                            <  2:113752/173‑1 (MQ=255)                                                                                                                                                                                    cTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCCGAGCCAACA‑AGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCCGCCCTTCAATGATCCGCCCGTCTTCGGTCCTCCATACGTTCCTTTCGTTCTCGTCGGCcgact                                                                                             >  1:190448/1‑297 (MQ=255)                                                                                                                                                                                                    cataGCTCAAATCCGATGACCTGTCACTGATATGGCTTCACTCCCTCGCCCCCCATCTGACCTTTCTACA‑TCAAAAAAAAAGCGACTCACCCGTCGCCGTAAAAATCAGTTTGCCCGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACA‑AGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGAt                                                                           <  2:190696/298‑1 (MQ=255)                                                                                                                                                                                                             aaatCAACAAACTGTCGCTGATATGGTTTCATTCCTTCGCTCCTCAGCTGTATTTGCGACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCGTCGGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACA‑AGATGAGTAGCTCTTCATGTGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCg                                                                  <  2:100332/298‑1 (MQ=255)                                                                                                                                                                                                              aTTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAACATCAGTTTGCCAGCGCCGCCTTCTGCGTCACTACCATGGATTCGATTCCCCTCGGGCCAGAGCCAACA‑CGATGAGTAGCTCTTCCTGGGTGAACGGCTCGCCTTCTCCCGTCACCTGCACTTCCATGATGCGCCCGTCTTCGGTCGTCCCTCTGTTCCTGTCGGTCTCTTCGGCCTAGTCTTCATCCTATTcacaatccccaaccc                                                                  >  1:401486/1‑286 (MQ=255)                                                                                                                                                                                                               ttCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACA‑AGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCCATGATGCGCCCGTCTTCGGTCATCACTACGTTCCTGTCGTCCTCTGCGCCAGAGTCTTCAACGTTTTCCAGATCCCAACccc                                                                  >  1:37973/1‑298 (MQ=255)                                                                                                                                                                                                                 cAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGCGCCAACA‑AGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTCCGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACCTATTCCAGATctttctcttttaa                                                              >  1:418819/1‑289 (MQ=255)                                                                                                                                                                                                                 cAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACA‑AGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGAt                                                                           <  2:418828/288‑1 (MQ=255)                                                                                                                                                                                                                              cTGAGATGGTTTCATGCCTTCGCTCCTCATCTTACTTTCCTACAG‑CGAAAAAAAAGCGACGCAGCAGTCGCCTCAAACATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACA‑AGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAAt                                                  <  2:129828/300‑1 (MQ=255)                                                                                                                                                                                                                                              ccTTCCCTCCTCATCTTACTTTTCCACAGCCAAAAAAAA‑TCGACTCAGCCGCCGCCTTAACAATCCGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGTGCCAGAGCCAACA‑AGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCgg                                 <  2:108173/301‑1 (MQ=255)                                                                                                                                                                                                                                                         cATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACA‑AGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCCCTTCAATGATGCGCCCGTCTTCGGTCATCAGTACGCTCATGTCGGTCTCTCCGGCAGAGTCTTCAACGTATTCCAGATCGCACAACGCTTCCCCGTTCCCACTTCCGACATaacatccggcacccacccc                       >  1:257239/1‑283 (MQ=255)                                                                                                                                                                                                                                                         cATCTTACTTTTCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACA‑AGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCGTCTCCCGTCCCCTGCACTTCCatgc                                                                                                                                                >  2:104815/1‑178 (MQ=255)                                                                                                                                                                                                                                                         cATCTTACTTTGCTACAGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACA‑AGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAatga                                                                                                                                                <  1:104815/179‑1 (MQ=255)                                                                                                                                                                                                                                                                     atACAGACAAAAAAAA‑GCGACTCAGCAGGCGCCTTAAAAATAAGTGTGCCAGCGCCGCCTGCTGCGGCGCTACAATGGATTCGAGTCCCCGCGGGCCAGAGCCAACA‑AGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCTTTCATCGGAtt           <  1:388197/299‑1 (MQ=255)                                                                                                                                                                                                                                                                         aGACAAAAAAAA‑GCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACA‑AGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCCGAGTCTTCAACGGCTGCCAGATCGCAAACCCCTTCGCCGTTCACCGTTCCGACACAACCCGCAGCCACACCCCCTTTCATCGGATTTGttt      >  1:201090/1‑301 (MQ=255)                                                                                                                                                                                                                                                                               aaaaaaAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGACTTATGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACA‑AAATGAGTAGCTCTTCATGGGTTAAAGGCTCGCCTTCTGCCGTCCCCTGCCCTTCAATGATGCGCCGGTCTTCGGTCATCACTACGTTCATTTCAGTCTCTTCGGCAGAGTCTTCAACGTATTCCAGCTCGCAAACCGCTCAGCCGTTCACAGTTACGACCGACACTGCGGCTCCCCTCCCTTTACTCGCATTGGTTTTCac  >  2:259947/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             GTGGCAATCGGGATCCCTTTGTAAGCAGGGCCAAACAGCAGATCGAACTCAATGCCGGAATCCACCAACGCTTCAGCGTAAAAACGGCCTAACAGTGCCAGATCGCGCCCGGTATTAAACAGCCCGGCGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACA‑AGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTCTTCAACGTATTCCAGATCGCAAACCGCTTCGCCGTTCACAATTCCGACAGAAACTGCGGCTACCATCCCTTTCATCGGATTGGTTTTCAG  >  NC_000913/3815533‑3816102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.