| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,502,577 | G→A | 25.2% | P637S (CCG→TCG) | fryA ← | putative PTS enzyme: Hpr, enzyme I and IIA components |
| Read alignment evidence... | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | change | freq | score | reads | annotation | genes | product | ||
| * | NC_000913 | 2,502,577 | 0 | G→A | 25.2% | 84.9 ‑0.5 ‑0.1 | 165 | P637S (CCG→TCG) | fryA | putative PTS enzyme: Hpr, enzyme I and IIA components |
| Reads supporting (aligned to +/- strand): new base (20/21): ref base (52/67): total (75/90) | ||||||||||
| Fisher's exact test strand distribution p-value = 5.90e-01 | ||||||||||
| Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.18e-01 | ||||||||||
Only 100 of 169 total aligned reads displayed.
AACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCGCCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCG‑CCGGAATACGCGGGCTAC‑TCATACTCAG‑CTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGG‑CTTTCACCGCCCAGTTCACCGCAAATGCC‑TACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCGGCGATACGCGCGGGTTATTACGATCGACCGCATACAGATACTGGGTCATATCGTTGGAGCCGATACTGAAGAAATCGACCTCATCGCAGAAGTGGTCGATGATGTAGCACACCGACGGAACTTCCACCATGAT > NC_000913/2502281‑2502877 | aTCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCACCAGTTCCCCACCCCCCTCGCTATCCATTTGACGAAGCTGGCTTCTCCCGT‑CCGGAATACGCGGGCaac‑tc > 2:860579/1‑300 (MQ=255) ctctTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCAGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAACTCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTACAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGACATTCACAAGCCTGACGCGCCAGTTCCCGACAACCCTCGGTATCCCATTTACGAAGCTGGCTTTTCCCCG‑CCGGAATACGCGGGAtgc‑tcata > 2:1752/1‑301 (MQ=255) ttCCCGCTGCCAGACATCTTCTTCCAGCGCAAACGGATGGTCAGTGCGCCCGTTGACGCCGAGGTTGCCGCACATGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCGCCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCG‑CCGGAATACGCGAGCtac‑tcatactc < 1:102967/301‑1 (MQ=255) ttCCCGCGTCCAGACATCTTCTTCCAGCCCAAACGGGTGTTCAGTGCGACCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCGCCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCG‑CCGGAATACGCGGGCtac‑tcatactc < 2:658996/301‑1 (MQ=255) ttCCCGCGGCAAGCCATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGGCCGTTAACGCCGAGGTTGCCGAACAGGAACTGGACCGCCTGCTCTTTATTGCTAACATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCTTGCGCACTGCGGCATTCACATGCCTGACGCGCCAGTTCCCGACCCGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCG‑CCGGAATACGCGAGCtac‑tcatactc < 2:14143/301‑1 (MQ=255) ttCCCGCCGGCAGACAGCATCTGCCACATCAAACGGAGTGTCCTTGGGCCCGTTAACGGCGAGGTTGACGCAAAGGAAATGGATCGTCTGCCCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCGTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCGCCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCG‑CCGGAATACGCGGGCtac‑tcatactc < 1:545419/301‑1 (MQ=255) tgcccCCTGCCCGACATGTGCTTCCATCCAAAAACGGTGTTAAGGCCGCCCGTTAACTCCGAGGTGGCCGCCAAGGAACTGGATCGCCTGCTCTGTATTGCTAAAAGCCTGATCAACAATGATAGTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTGCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCCCTGCGGCATTCACATGCCTGACGCGCCAGTTCCCGACACGCCGCGCTATCCAGTTGACGAAGCTGGCTGTTCACCG‑CCGGAATACGCGGGCtac‑tcatactc < 1:963210/299‑1 (MQ=255) ccAGACATCTTCTTCCAGCTCAAACGGATGTTCAGGGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCGTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCGCCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCG‑CCGGAATACGCGGGCTAC‑TCATACTCAG‑CTCATc < 2:414283/300‑1 (MQ=255) tataaaagacaagcctaaaCGGCTTTTCAGTGCCCCCGTTAACGCCGGTGTTGCCGCACAGGAACTGGATCGCCTGATCTTTAGTGCTAAAATCCTGATCAACAAAGATATTCTCCGGCGCCAGCAGTGGGCGAACGTCTTCGTCCGGCGGAAAGGCTGTGAGTCACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCGCCAGTTCCCGCCACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCG‑CCGGAATACGCGGGCTAC‑TCATACTCAG‑CTCATCCAGgcc < 2:1308289/285‑1 (MQ=255) tcttctTCCAGCTCAAACGGATGTTCAGTGCGACCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAACTCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCGCCAGTTCCCGACACGCCTCGCTATCAAGTTGACGAAGCTGGCTTTTCACCG‑CCGGACTACGCGGGATAC‑TCATACTCCA‑CTCATCCAGgacc > 2:443763/1‑300 (MQ=255) tctgcCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCTCAAAGGAACTGGATCTCCTGCTCGTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCGCCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCG‑CCGGAATACGCGGGCTAC‑TCATACTCAG‑CTCATCCAGgcccagcc < 1:1309340/301‑1 (MQ=255) acgttaaaCGCAGGGTCAGTGGGCCCGTTAACGCCGAGGTTGCCGAACAGGAACTGGATCTCCTGCCCTTTATTGCTAAAATCCTGCTCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTTCGCACTGCGGCATTCACATGCCTGACGCGCCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCG‑CCGGAATACGCGAGCTAC‑TCATACTCAG‑CTCATCCAGGCCCAGCCCAAGCa < 1:1457027/296‑1 (MQ=255) gCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGATCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTACAGACGGTGCGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCCGACGCCCCAGTTCCCGACACGCCCCGCTACCCAGTTCACGAAGCTGGCTTTTCGCCG‑CCGGCAACCGCGGGCTCC‑CCCTACTCGC‑CTCACCCaccacctgcccctccaa > 1:1308240/1‑285 (MQ=255) cAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCGCCAGTTCCCGACACGCCTCGCTATCCATTTGACGACGCTGGCTTTTCACCG‑CCGGAATACGCGGCCCAC‑CCATCCTCAG‑CCCATCCAGGCCCCGCCCAAGc > 1:564911/1‑296 (MQ=255) gATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCATGCGCACTGCGGAATGCCCATGACTGCAGCGCAAGTTCCCGACACCACACGCTATCCAGTTCCAGAAGCATGCTTTTCACCG‑CCGAAACACGCGGGGTCC‑CCATCCTCAC‑CTACTCCAggacaccccccaacaagagccgcca > 1:1168821/1‑278 (MQ=255) tGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCACTTTCCTTAGCACTGCGGCATTCACATGCCTGACGCGCCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGATTATCACCG‑CCGGAATACGCAGACTCC‑TCATACTCAG‑CTCATCCCGGCCCAGCCCAAGCAGAAGCgaccgca > 2:1497769/1‑295 (MQ=255) tGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGTCATTCACATGCCTGACGCGCCAGTTCCCGACACGCCTCCCTATCCAGTTGACGAAGCTGGCTTTTCACCG‑CCGGAATACGCCAGCACC‑TCCTACTCAG‑CTCATCCAGACCCAGCCCAAGCAGTATCAGCAGaa > 2:1098110/1‑300 (MQ=255) tGTTCAGGGCGCCCGTTAACGCCGGGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTAGTGCTAAAATCCTGATCAACAAAGATATTCGCCAGCGCCAGCAGTGGGCGAACGTCTGCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCGCCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCG‑CCGGAATACGCGGGCTAC‑TCATACTCAG‑CTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGAt < 1:1382006/301‑1 (MQ=255) gTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATAAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCCCTGCGGCATTCACATGCCTGACGCGACAGTTCGCGACACTACTCGCTATCCCGTTGACGCAGCTGCCTTTTCCCCG‑CCCGAATAAGCGGGCTAC‑TCATACTCAG‑CTCCTCCAGGCCCAGCCCAAGCAGTCGCGGCAGATa > 2:782946/1‑301 (MQ=255) ttcgtgggccgggtgaagcatggctgtacGCACATGACCTGGATCGCCTGCTCTTGATTGCTAAAATCCGGCTCAACAAAGCGACTCTCGAGCGCCAGCAGTGGGCGAACGTTCTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTGAATGTCCTGCGCACTGCGGCATTCACATGCCGGACGCGCCAGTTCCCGACACGCCGAGCTAGCCAGTTGACGAAGCTGGCTGTTCACCG‑CCGGAATACGCGAGCTAC‑TCATACTCAG‑CTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAAc < 1:941135/273‑1 (MQ=255) gTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCGCCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCG‑CCGGAATACGCGGGCTCA‑CCATCCTCAG‑CCCATCCCGGCCCAGCCCAAGCCTGAGCGGCAGATACCgg > 2:421916/1‑300 (MQ=255) gTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGAGCACTGCGGCATTCACATGCCTGACGCGCCAGTTCCCGACACGCCTCGCTCTCCCGTTGCCGACGCTGGCTTTTCACCG‑CCGACACACCCGCGCCAC‑TCATCCCCAC‑CCCATCCACGCCCACCCCCACCAGTaaccctgtccctcata > 1:1553397/1‑286 (MQ=255) ccGTTACCGCCGAGGTTGCCGCACAGGAACTGGATCGCCTTCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCGCCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCG‑CCGGAATACGCGGGCTAC‑TCATACTCAG‑CTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGG‑CTTTCAc < 2:1447191/301‑1 (MQ=255) ccGTTAACGCCGAGGTGGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGGGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCGCCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCG‑CCGGAATACGCGGGCTAC‑TCATACTCAG‑CTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGG‑CTTTCAc < 1:377764/301‑1 (MQ=255) ggAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCGCCAGTTCCCGACACGCCTCGCTCACCAGTTGACGCAGCTGGCTTTTACCCG‑CCGGAATACGCCGGCTAC‑TCACCCTCCG‑CTCATCCACGCCCAGCCCACGCAGCGCCGGCCCATAACGG‑CTTTCACC‑CCCACCTCAcccgcaacccccca > 1:845655/1‑288 (MQ=255) aaCTTGCTCGCCTGCTCTTTATTGCTAAAATCCTGAACAACAAAGTTACTCTCCAGCGCCAGCAGTGGGCTAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACGGCGGCATTCACATGCCTGATGCGCCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCG‑CCGGAATACGCGAGCTAC‑TCATACTCAG‑CTCATCCAGGCCCATCCCAAGCAGTAGCGGCAGATAACGG‑CTTTCACCGCCCAGTTCACCGCAAATGCC‑TAccc < 2:867447/301‑1 (MQ=255) aaCTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACCAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCTCACTGCGGCATTCACATGCCTGACGCGCCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCG‑CCGGAATACGCTGGCTAC‑TCATACTCAG‑CTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGG‑CTTTCACCGCCCAGTTCACCGCAAATGCC‑TAccc < 2:1172140/301‑1 (MQ=255) cTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTACAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCCCATGCCTGCCGCTCCCGTTCCCGACACGCCTCGCTATCCAGTTGACGCAGATGGCTTTTCCACG‑CCGAAATACGCGGACTAC‑TCATGCTCCG‑CTCATCCAGGCCCAGCCCAAGCAGTAGCGGACGATAACGG‑CTTTCACCCCCCCATTCCCCGCAACTGCC‑CAccc > 2:1042519/1‑299 (MQ=255) cTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGTTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCGCCAGTTCCCGACACGCCTCGCTATCCAGTTTCCGAAGCCGGCTTTTCACCG‑CCGGAATACGCGGGCTAC‑TCATACTCAG‑CTCATCCAGGCCCAGCCCAAACAGCAGCGGACGCTAACGG‑CTTTCCACGCCCAGTTAACCGCGAATGCC‑TCACCAt > 2:1436813/1‑301 (MQ=255) cTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCGCCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCG‑CCGGAATACGCGGGCTAC‑TCATACCCAG‑CTCATCCAGGCCAAGCCCCAGCAGTAGCGGCAGCTCACGG‑CTTTCCCCGCCCCGTTCCCCCCAAATGCC‑TACCCa > 1:106167/1‑300 (MQ=255) ggATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCGCCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCG‑CCGGAATACGCGGGCTAC‑TCATACTCAG‑CTCATCCAGgcccagcc > 2:554404/1‑240 (MQ=255) gCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCGCCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCCCCG‑CCGGAATACGCGAGCTAC‑CCCTACGCAG‑CTCATCCAGGCCCAGCCCAAGCAGTAACGGCCGATAACGG‑CTGTCGCCGCCCAGTGCCCCGCAAAGCCC‑TAccgctttacaccact > 1:226319/1‑287 (MQ=255) gCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCGCCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCG‑CCGGAATACGCGGGCTAC‑TCATACTCAG‑CTCATCCAGGCCCAGCCCAAGCAGTCGCGGCAGCTCACGG‑CCTTCCCCGCCCAGCTCCCCGCAACTGCC‑TACCCCTTTGCCCCg > 1:197679/1‑298 (MQ=255) gCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCGCCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCG‑CCGGAATACGCGGGCTAC‑TCATACTCAG‑CTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATCACGG‑CTTTCACCCCCCAGTTCACCGCAAATGCC‑TACCCTTTTGCCCCg > 2:419787/1‑298 (MQ=255) gCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCGCCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCG‑CCGGAATACGCGGGCTAC‑TCATACTCAG‑CTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGG‑CTTTCACCGCCCAGTTCACCGCAAATGCC‑TACCCATTTGCCCCg > 2:521099/1‑298 (MQ=255) gCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCGCCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCG‑CCGGAATACGCGAGCTAC‑TCATACTCAG‑CTCATCCAGGCCCAGCCCAAGCCGTAGCGGCAGCTAACGG‑CTTTCACCGCCCCGTTCACCGCAAATTCC‑TACCCCTTTGCCCCGCtt > 2:241316/1‑300 (MQ=255) gCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCGCCACTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTGTCACCG‑CCGGAATACGCGCGCTAC‑TCCTACTCCG‑CTCACCCAGGCCCAGCCCCAGGAGTAGCGGCAGAGACCGG‑CGTCCCCCGCCGAGTCCCCCCCAAATGCCACACCCA‑TTGCCCCCCta > 1:1350416/1‑300 (MQ=255) ctTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCGCCAGTTCCCGACACCCCTCCCTTTCCATTTG‑CCAAGCTGGGTTTTTCCCGCCCGGAATCCGCGGGC‑AC‑CCCTACTCAGCCCCACCCAGCCCCA‑CCCCAGCCGTAGCCGCAGGTAACGGACTTTCACC‑CCCCGCTCCCCGCACATGCC‑TAccccctttggccgcctgatg > 2:433299/1‑284 (MQ=255) taagatgcggatatgagcatgcaaAGCTAGTCTCCAGGGCCAGCCGGTGTCTAAAGCCGTCTCTCGGCGTAACGGCGGGTAGTAACGCGTCAATTTCCGTCGCACTGCGGCATTATCATGCCTGACGCGACAGTTCCCGACACGCCTCGCTAGTCAGTTGACGAAGCTGGCTCTGCCCCG‑CCGGAATACGCGGGCTAC‑TCATACTCAG‑CTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGG‑CTTTCACCGCCCAGTTCACCGCAAATGCC‑TACCCATTTGCCCCGCTGATgcgc < 1:1350721/280‑1 (MQ=255) gCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGCGTAACGCTTCAATTTCATGCGCACTGCGGCATTCACATGCCTGACGCGCCAGTTCCCGACACGCCTCGCGCTCCAGTTGACGAAGCTGGCTTTTCCCCG‑CGCGAATACGCGGGCtac‑tcatactc > 2:1320045/1‑202 (MQ=255) gCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCGCCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCG‑CCGGAATACGCGGGCTAC‑TCATACTCAG‑CTCATCCAGgcc > 2:484909/1‑216 (MQ=255) gCTAAAACCCTTATCAACAAAGAGATCCTCCAGGGCCATCAGTTGGCGAACGTCTTCTTCCGGCGTAAAGTCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCGCCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCG‑CCGGAATACGCGGGCtac‑tcatactc < 1:1319996/202‑1 (MQ=255) cTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCGCCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCC‑CCGGAATACGCGGGCTAC‑TCATACTCAG‑CTCATCCAGGCCCCGCCCAAGCAGTAGCGGCAGATAACGG‑CTTTCACCCCCCAGCTAACCCCAAATGCC‑TCCCCATTTGCCCCCCtcacccccacggg > 1:1219754/1‑289 (MQ=255) caccTGATCAACAAAGAGCTTCCCCCGGCACAGACGTGGGCGATCGTCGTCTTCCGGCGTAAAGGCGGTGGGGAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCGCCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCG‑CCGGAATACGCGGGCTAC‑TCATACTCAG‑CTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGG‑CTTTCACCGCCCAGTTCACCGCAAATGCC‑TACCCATTTGCCCCGCTGATGCGCAGTGGTAACt < 1:1352047/299‑1 (MQ=255) tCCTGATCAACAAAGATATTCTCCGGCGCCGGCAGTGGGCGACCGTCTGCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCGCCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCG‑CCGGAATACGCGGGCTAC‑TCATACTCAG‑CTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGG‑CTTTCACCGCCCAGTTCACCGCAAATGCC‑TACCCATTTGCCCCGCTGATGCGCAGTGGTAACTa < 2:812615/301‑1 (MQ=255) tCCTGATCAAAAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGGGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCGCCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCG‑CCGGAATACGCGAGCTAC‑TCATACTCAG‑CTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGG‑CTTTCACCGCCCAGTTCACCGCAAATGCC‑TACCCATTTGCCCCGCTGATGCGCAGTGGTAACTa < 1:952400/301‑1 (MQ=255) cccttttCAACAACTACATTCTCCGGCGCCGGCAGTGGGCGAACTCCCTTCTCCGGCTTAAAGGCGGTGGGTAACGCCTCAATTTCCGGCGCACTGCGCCATTCACATGCCTGACGCGCCACTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCG‑CCGGAATACGCGAGCGAC‑TCATACTCAG‑CTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGG‑CTTTCACCGCCCAGTTCACCGCAAATGCC‑TACCCATTTGCCCCGCTGATGCGCAGTGGTAACTa < 1:808410/295‑1 (MQ=255) ataaacaaACAACTTCTCAAGTGCCAGAAGTGGTCGAACTGTTTCTTCCGGCTTACAGGAGATGAGTAACCCTTAAATTTCCTGCGCACTGCGGCATTTACATGCCTGACGCGCCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCG‑CCGGAATACGCGGGCTAC‑TCATACTCAG‑CTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGG‑CTTTCACCGCCCAGTTCACCGCAAATGCC‑TACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTtgc < 2:845234/298‑1 (MQ=255) caaAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCGCCAGTTCCCGACACGCCTCGCTATCCAGTTGACGCAGCTGGCTTTTCACCG‑CAGGAATACGCGGGCTAC‑TCCTACTCAG‑CTCATCCAGACCCAGCCCACGCAGTCACGGCAGGTACCGG‑CTTTCACCGCCCCGTTACCAGCAACTTCC‑TACCCCTTAACCCCGcccttcggcatttccctcgtcgccacccc > 2:1350771/1‑272 (MQ=255) aaGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCGCCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCG‑CCGGAATACGCCGGCTAC‑TCATACTCAG‑CTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGG‑CTTTCACCCCCCAGTTCACCCCAACTGCC‑TACCCATCTACCCCGCTGacaccccgtcgtcaccagttgccacacca > 1:248541/1‑273 (MQ=255) aGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCGCCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCG‑CCGGAATACGCGAGCTCC‑TCATACTCAG‑CTCATCCAGCCCCAGCCCACGCAGCAGCGGCAGATAACGG‑CTTTCACCCCCCAGTTCCCCGCCACTGCC‑TACCCCTTTGCCCCCCTGCTCCGCCGTGGAACCACTTTCCTGCAAcc > 1:1403749/1‑299 (MQ=255) tatTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCGCCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCG‑CCGGAATACGCGGGCTAC‑TCATACTCAG‑CTCATCCAGGCCCAGCCCACGCCGTAGCGGCAGCTAACGG‑CTTTCACCGCCCAGTTCACCCAAAATGCC‑TACCCATTTGCCCCGCTGATGCGCCTTGGTAACtcctgtcccttataccc > 2:1153593/1‑284 (MQ=255) cgcCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCGCCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCG‑CCGGAATCCGCGGGCTAC‑TCCTACTCAG‑CTCATCCAGGCCCAGCCCCAGCAGTACCGCCAGCTAACGG‑CTTTCCCCGCCCAGTTCCCCCCAAAAGCC‑TCCCCATTTGCCCCCCTGATGCGCCGCGGTCCATCTTTGATGCCACATgcgacggacccca > 1:1183074/1‑291 (MQ=255) ccatcgctGGGCGACCCGCTGCTTCCGGCGTAAGGGCGGTGCGTAAGCCGTCAATTCCCGGCGCAGTGCGGCGTTCACATGCCTGACGCGCCAGTTCCCGACACGCCGCGCTGTCGAGTTGCCGAAGCTGGCTTTTCACCG‑CCGGAATACGCGGGCTAC‑TCATACTCAG‑CTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAGCGG‑CTTTCACCGCCCAGTTCACCGCAAATGCC‑TACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATgg < 1:419781/294‑1 (MQ=255) aaCGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCGCCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCG‑CCGGAATACGCGAGCTAC‑TCATACTCAG‑CTCATCGAGGCCCAGCACAAGCAGTAGCGGCAGATAACGG‑CTTTCACCGCCCAGTTCACCGCAAATGCC‑TACCCATTTGCCCCGCTGATGCGCAGCGGTAACTCTTTGACGCAACATGCGACGGAACGATGGCGTAATAGGGTta > 2:109905/1‑301 (MQ=255) gTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCGCCAGTTCCCGACACGCCTCTCTATCCAGTTGACGAAGCTGGCTTTTCACCG‑CCGGAATACGCGGGCTAC‑TCATACTCAG‑CTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGG‑CTTTCACCGCCCATTTCACCCCAAATGCC‑TACCCCTTCGCCCCCCTGCTTCGGAGTGGTCAACATTCGCTGAAACATTCGCAGCaagcatgacacaaacgggtcat > 2:705423/1‑279 (MQ=255) tcttctTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCGCCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCG‑CCGGAATACGCGGGCTAC‑TCATACTCAG‑CTCCTCCAGGCCCAGCCCAAGCAGTGGCGGACGATAACGG‑CTTTCACCGCCCAGTTCCCCGCACATGCC‑TCCCCATTTGCACCGCTGATCCGCCGCGGTAACTATTTGCGCCAACATGCGCCGGCACGATGGCGTACTCGTGAtataca > 1:283705/1‑299 (MQ=255) tcttctGCCGCCGTAAAGGCGGTGATTACCGCTTCCATGTCCTGCGCCCTGCGGCAATCACACTCCTGACGCGCAAGTTCCCGACACGCCTCGCTATCCAGTTTACGAATCTGGCCTTTCACCG‑CCGTAATACGCGGGCTAC‑TCATACTCAG‑CTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGG‑CTTTCACCGCCCAGTTCACCGCAAATGCC‑TACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTtatat < 2:279213/300‑1 (MQ=255) cttcgtCCGGCGTAAAGGCGGGGATTCACGCTGCAACTGCCCGCGCCCGCGGGCATTCCCTGGCCTGCCCCGCCAGTTCCCGACACCCGTCGCTATCCAGTTGACGAAGCTTGCTTGTCACCG‑CCGGAATACGCGGGCCAC‑TCATCCTCAG‑CTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGG‑CTTTCACCGCCCAGTTCACCGTAAATGCC‑TACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTtatatgg < 2:1357221/301‑3 (MQ=255) gCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCGCCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCG‑CCGGAATACGCGGGCTAC‑TCATACTCAG‑CTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGCTAACGG‑CTTTCACCGCCCCGTTCACCGCAAATGCC‑TACCCATTTGCCCCGCTGCTCCCCCGTGGTAACTATTTGCAGACACCTGCCCCGGACCGATgcgtcactccggttttatacggtcgata > 2:450597/1‑273 (MQ=255) cGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCGCCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCG‑CCGGAATACGCGGGCTAC‑TCATACTCAG‑CTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGG‑CTTTCACCGCCCAGTTCACCGCAAATGCC‑TACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCGGCGATAcg < 2:948976/301‑1 (MQ=255) gAGTAACGCTTCACTTTCCTGCGCACTGCGGCATTCACATGCCTGACGCGCCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCG‑CCGGAATACGCGAGCTAC‑TCATACTCAG‑CTCATCCCGGCCCAGCCCAAGCAGTCGCGCCAGATCCCGG‑CTTTCACCGACCAGTTCACAGCAAATGCC‑TACCACTTTCCCCCGCTGATGCCCAGTGCTCACGTTTTGCTCCAACACGCGCAGGCCCGATGGCGTCATCGGGTTCTCACTAGCCGATACTCCCGGGttatta > 1:584341/1‑301 (MQ=255) gAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCGCCAGTTCCCGACACTCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCG‑CCGGAATACGCGAGCTAC‑TCATACTCAG‑CTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGG‑CTTTCACCGCCCAGTTCACCGCAAATGCC‑TACCCATTTGCCCCGCTGATGCGGCGTGGTAACt > 2:912901/1‑232 (MQ=255) gAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCGCCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCG‑CCGGAATACGCGAGCTAC‑TCATACTCAG‑CTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGG‑CTTTCACCGCCCAGTTCACCGc < 2:1222152/191‑1 (MQ=255) gttagcGCTGCAATTTCCTGCGCACGGCGGCATTCACATGCCTGACGCTCCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGC‑TTTCACCG‑CCGGAATACGCGGGCTAC‑TCAGACTCAG‑CTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGG‑CTTTCACCGCCCAGTTCACCGCAAATGCC‑TACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCGGCGATACGCGCGGGTTATTAc < 2:162344/295‑1 (MQ=255) gTAACGCCTCAATTCCCTCGCCCCTGCCGTATTCACACCCCTGACTCGCCAGTGACCGACACGCCTCGCTATCCAGTTGTCTAAGCTGGCGTTTCCCCG‑ACGGAATACGCGGGCTAC‑TCATACTCAG‑CTCATCCAGGCCCAGCCCAAGCAGGAGCGGCAGATAACGG‑CTGTCACCGCCCAGGTCACCGGAAATGCC‑TACCCATTGGCCCCGCTGAGGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCGGCGATACGCGCGGTTTATTAc < 1:782933/300‑1 (MQ=255) tcaCGCGTCAATTGCCTGCGCACTGCGGCAATCACATGCCGGACGCGCCAGTTCCCGACACGCCTAGCTAACCAGTTGACGAAGCTGGCGTTTCACCG‑CCGGAATAAGCGGGCTAC‑TCATACTCAG‑CTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGG‑CTTTCACCGCCCAGTTCACCGCAAATGCC‑TACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCGGCGATACGCGCGGGTTATTAc < 2:1333126/297‑1 (MQ=255) aacgtgccagctctccccgccacgCGGCATTCACGTGCCGGACGCGCCAGTTCCCGGCACGCCTCGCTATCCAGTTGACGAAGCGCGCTGTTCACCG‑CCGGAATACGCGGGCTAC‑GCATACTCAG‑CTCATCCAGGCCCAGCACAAGCAGTAGCGGCAGATAACGG‑CTTTCACCGCCCAGTTCACCGCAAATGCC‑TACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCGGCGATACGCGCGGGTTATTACGAt < 1:1436758/278‑1 (MQ=255) tCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCGCCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCG‑CCGGAATACGCGGGCTAC‑TCATACTCAG‑CTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGG‑CTTTCACCGCCCATTTCACCGCAAATGCC‑TACCCATTTTCCCCGCTGCTGCGCAGTGGTAACTCTTTGCTGCAACATGCGCAGGCACGATGTCGTAATCGGGTTATATAGCGGGGATACGCGCGGGTTATTACGATCGa > 2:158014/1‑298 (MQ=255) tCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCGCCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCG‑CCGGAATACGCGGGCTAC‑TCATACTCAG‑CTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGG‑CTTTCACCGCCCAGTTCACCGCAAATGCC‑TACCCCTTTCCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGACCGCTGGCGTAATCGGGTTATCTAGCGGCGATCCGCCGGGGTGATTACGAtgtacc > 2:34969/1‑295 (MQ=255) aTTTCCTGCGCACTGCGGCATTCACATGCCTGACGCGCCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCG‑CCGGAATACGCGAGCTAC‑TCATACTCAG‑CTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGG‑CTTTCACCGCCCAGTTCACCGCAAATGCC‑TACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCGGCGATACGCGCGGGTTATTACGATCACCCGCaa > 2:23022/1‑300 (MQ=255) ttCCTGCGCACTGCGGCATTCACATGCCTGACGCGCCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCG‑CCGGAATACGCGGGCTAC‑TCATACTCAG‑CTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGG‑CTTTCACCGCCCAGTTCACCGCAAATCCC‑TACCCCTTTGCCCCGCTGATGCGCAGCGGTAACTATTTGCTGCAACATGCGCCGGAAAGATGGCGTACTCGGGTTATATCGCGGCGATACGCGCGGGGTATTCCGATCGACCGCGTa > 2:278739/1‑300 (MQ=255) ttCCTGCGCACGGCGGCATTCACATGCCTGACGCGCCAGTTCCCGACACGCCTCGCTATCCAGTGGACGAAGCTGGCTTTTCACCG‑CCGGAATACGCGGGCTAC‑TCATACTCAG‑CTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGG‑CTTTCACCGCCCAGTTCACCGCAAATGCC‑TACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCGGCGATACGCGCGGGTTATTACGATCGACCGCATAc < 2:1183118/301‑1 (MQ=255) cggccacctgtgcagtccagtgtccgACGCGCCCGCGCCCGACACCCCCCGCCAGCCAGTTGACGAAGCTGGCGTTCCACCG‑CCCGAAGACGCGGGCTAC‑CAACACTCAG‑CTCAGCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGG‑CTTTCACCGCCCAGTTCACCGCAAAGGCC‑GACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCGGCGATACGCGCGGGTTATTACGATCGACCGCATACAGAt < 1:450589/276‑1 (MQ=255) gcgcACTGCGGCATTCACATGCCTGACGCGCCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCG‑CCGGAATACGCGAGCTAC‑TCATACTCAG‑CTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATACCGG‑CTTTCACCGCCCAGTTCACCGCAAATGCC‑TACCCATTTGCCCCGCTGCTCCGCAGTGGTCACTATTTGATGCAACATGCTCCGGACCGATGGCGTAATCGGGTTATACTGCGGCGATACGCCCGGGCTCTTACTATCggacgccagacgaaa > 1:472685/1‑287 (MQ=255) tccACGGCGGCATTCAACTGCCTGACGCTCCAGCTACCGACACTCCTCGCTATCCAGGTTACGAAGCTGGCTGTTCACCG‑CCGGAATACGCGGGCTAC‑TCATACTCAT‑CTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGG‑CTTTCACCGCCCAGTTCACCGCAAATGCC‑TACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCGGCGATACGCGCGGGTTATTACGATCGACCGCATACAGATAc < 2:1100950/299‑1 (MQ=255) cccTGCGGCTTTCACAGTCACGCCGCTCCAGCTCCCTACACCCCCCGCGATCCAGGTTCGCAAGTCGGCTCTTCACCG‑CCTGAATACGTGGTATTC‑TCATACTCCG‑CTCATCCAGGCCCATCCCAAGCAGTAGCGGCAGATAACGG‑CTTTCACCGCCCATTTCACCGCAAATGCC‑TACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCGGCGATACGCGCGGGTTATTACGATCGACCGCATACAGATACTg < 1:1098068/299‑1 (MQ=255) cTGCGGCATTCACATGCCTGACGCGCCAGTGCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCG‑CCGGAATACGCGAGCTAC‑GCATACTCAG‑CTCATCACGGCACAGCCCCAGCAGTAGCGGAAGATAACGG‑CTATCACCGCACAGTTCCCCGCAAATGCC‑TACCCCTTTGCACCGCTGATGCGCCGTGGAAACTATTtgcg > 2:1511798/1‑213 (MQ=255) ttCACATGGCTGGAGCCCCATTTCCCGACACGCCTCGGCATCAAGGTTACGAAGCCGGCTGTTCACCG‑CCTGACTTCGCGGGCTAC‑TCAGAATCAG‑CTCATCCAGGCCCAGCCCAATCAGTAGCTGCAGATAACGG‑CTTTCACCGCCCAGTTCACCGCAAATGCC‑TACCCAGTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCGGCGATACGCGCGGGTTATTACGATCGACCGCATACAGATACTGGGTCATATCg < 2:1545461/301‑1 (MQ=255) cacaTGCCTGACGCGCCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCG‑CCGGAATACGCGGGCTAC‑TCATACTCAG‑CTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGG‑CTTTCACCGccc < 2:72431/146‑1 (MQ=255) cacaTGCCTGACGCGCCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCG‑CCGGAATACGCGGGCTAC‑TCATACTCAG‑CTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGG‑CTTTCACCGccc > 1:72429/1‑146 (MQ=255) tGCCTGACGCGCCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCG‑CCGGAATACGCGGGCTAC‑TCATACTCAG‑CTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGG‑CTTTCACCGCCCAGTTCACCGCAAATGCC‑TACCCATTTGCCCCGCTGATGCGCAGTGGTAACTa > 1:633177/1‑194 (MQ=255) gTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCG‑CCGGAATACGCGGGCTAC‑TCATACTCAG‑CTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGG‑CTTTCACCGCCCAGTTCACCGCAAATGCC‑TACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCGGCGATACGCGCGGGTTATTACGCTCGACCGCATACAGATACTGGGTCATATCGTTGGAGCCGATACTgaagca > 2:942378/1‑299 (MQ=255) tacccGACCCGCCTCGCTACCCAGTTGACGAACCGCGCTGTTCACCG‑CCGGACAACGCGGGCTAA‑CCCTCCTTAG‑CTCATCCAGGCCCAGCCCAAGCAGGAGCTGCAGACAACGT‑CTTTAAACGTCCGGTCCACCGCAAATGCC‑CACTCAGTTGCCCCGCTGTGGCGCAGTGGTAACGATTTTCTGTAACATGTGCAGGAACGATGGCGTAATCGGGTTATATCGCGGCGATACGCGCGGGTTATTACGATCGACCGCATACAGATACTGGGTCATATCGTTGGAGCCGATACTGAAGaaa < 2:1216979/299‑1 (MQ=255) tatacttCCCGTTGACAAAGCTTGCCTTTCACCG‑CCGGACTCCGCGATCTCC‑TCACACCCAG‑CTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGG‑CTTTCACCGCCCAGTTCACCGCAAAGGCC‑TACCCAGTTGTCCGGCTGAGGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCGGCGATACGCGCGGGTTATTACGATCGACCGCATACAGATACTGGGTCATATCGTTGGAGCCGATACTGAAGAAATCGACCTCATCGc < 1:497097/295‑1 (MQ=255) cTATCCAGTTGACGAAGCTGGCTTTTCACCG‑CCGGAATACGCGAGCTAC‑TCATACTCAG‑CTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGG‑CTTTCACCGCCCAGTTCACCGCAAATGCC‑TACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACCTGCGCCGGAACGCTGGCGTAATCGGGTTATCTAGCGGCGATACGCGCGGGTTGTTACGATCGACCGCATCCAGCACATGGATAATATCGTTGGAGCCGATACTGAAGACATCGACCTCATCGc > 2:497106/1‑298 (MQ=255) ctcccatacaCGAAGCTGTCTGTTCCCCG‑CCGGAATCCGCAAGCCAC‑TCACACTCAG‑CTCATCCCGGCCCAGGCTAAGCATGAGCGGCAGAGAACGG‑CTTTCACCGCCCCGTGCCCCGCAAATGCC‑TACCCAGTTGCCCCGCTGATGCGCAGTGGTAACTAGCTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCGGCGATACGCGCGGGTTATTACGATCGACCGCATACAGATACTGTGTCATATCGTTGGAGCCGATACTGAAGAAATCGACCTCATCGCAGAAg < 2:285091/292‑1 (MQ=255) aTCCAGTTGACGAAGCTGGCTTTTCACCG‑CCGGAATACGCGGGCTAC‑TCATACTCAG‑CTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGG‑CTTTCACCGCCCAGTTCACCGCAAATGCC‑TACCCATTTGCCCCGCTGATGCGCCGTGGTCACTATTTGCTGCAACATGCGCAGGAACGATGGCGTACTCGGGTTCTATAGCGGCGATACGCGCGGGTTATTAc > 1:286071/1‑230 (MQ=255) aTCCAGTTGACGAAGCTGGCTTTTCACCG‑CCGGAATACGCGGGCTAC‑TCATACTCAG‑CTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGG‑CTTTCACCGCCCAGTTCACCGCAAATGCC‑TACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCGGCGATACGCGCGGGTTATTACGATAGACAGCATACAGGTACTGGGTCCTATCGTTGGAGCCGATACTGAAGACAACGACCTCATCGCAGaaa > 2:1273751/1‑300 (MQ=255) aTCCAGTTGACGAAGCTGGCTTTTCACCG‑CCGGAATACGCGAGCTAC‑TCATACTCAG‑CTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGG‑CTTTCACCGCCCAGTGCACCGCAAATGCC‑TACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCGGCGATACGCGCGGGTTATTACGATCGACCGCATACAGATACTGGGTCATATCGTTGGAGCCGATACTGAAGAAATCGACCTCATCGCAGAAg < 2:226324/301‑1 (MQ=255) aTCCAATTGACCAACCTGGCCGTCCACCG‑CCGGAATACGCGGGCCAC‑TCCTACTCTG‑CCCAGCCAGCCCCAGCCCAAGCAGTAGCGGCAGATAACGG‑CTTTCACCGCCCAGTTCACCGCAAATGCC‑TACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCGGCGATACGCGCGGGTTATTACGATCGACCGCATACAGATACTGGGTCATATCGTTGGAGCCGATACTGAAGAAATCGACCTCATCGCAGAAg < 1:824778/301‑1 (MQ=255) ccAGTTGACGAAGCTGGCTTTTCACCG‑CCGGAATACGCGAGCTAC‑TCATACTCAG‑CTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGG‑CTTTCACCGCCCAGTTCACCGCAAATGCC‑TACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCGGCGATACGCGCGGGTTATTACGATCGACCGCATAc < 2:324966/242‑1 (MQ=255) ccAGTTGAAGAAGCGGGTTGTTCACCG‑CCGGAATACGCGGGCTAC‑CCATACTCAG‑CCCATCCAGGCCCAGACCAAGAAGGAGCGGCAGATAACGG‑CTTTCACCGCCCAGTTCACCGCAAATGCC‑TACCCATTTGCCCCGCTGAGGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCGGCGATACGCGCGGGTTATTACGATCGACCGCATACAGATACTGGGTCATATCGTTGGAGCCGATACTGAAGAAATCGACCTCATCGCAGAAGt < 1:942360/300‑1 (MQ=255) aCGAAGCTGGCTTTTCACCG‑GCGGAATACGCTGGCTAC‑TCATACGCAG‑CTCCTCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGG‑CTTTCACCGCCCAGTTCACCGCAAATGCC‑TACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCGGCGATACGCGCGGGTTATTACGATCGACCGCATACAGATACTGGGTCATATCGTTGGAGCCGATACTGAAGAAATCGACCTCATCGCAGAAGTGGTCgat < 2:330961/300‑1 (MQ=255) tcTTTTCACCT‑CCGGAAAGCGCCGGCTTA‑TCATACGCAG‑TGAATCCAGCGCCAGCCCAAGCAGGAGCGGCAGATAACGG‑CTGTCACCGCCCAGTTCACCGCAAATGCC‑TACCCAGTTGCCCCGCTGATGCGCAGTGGTAACGATTTGCTGCAACATGCGCAGGAACGATGGCGTAAGCGGGTTATATAGCGGCGATACGCGCGGGTTATTACGATCGACCGCATACAGATACTGGGTCATATCGTTGGAGCCGATACTGAAGAAATCGACCTCATCGCAGAAGTGGTCGATGATGTAGcac < 1:34967/300‑1 (MQ=255) cgtttCACCG‑CCGGAATACGCGAGCTAC‑TCAGACTCAG‑CTCAACCAGGCCAAGCCCAAGCAGGAGCGGCAGATAACGG‑CTGTCACCGAACAGTTCAACGAAAATGCC‑TACCCATGTGCACCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCGGCGATACGCGCGGGTTATTACGATCGACCGCATACAGATACTGGGTCATATCGTTGGAGCCGATACTGAAGAAATCGACCTCATCGCAGAAGTGGTCGATGATGTAGcac < 2:573808/298‑1 (MQ=255) gAATACGCGAGCTAC‑TCATACTCAG‑CTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGG‑CTTTCACCGCCCAGTTCACCGCAAGTGCC‑TACCCATTTGCCCCGCTGATGCTCAGTGGTAACTATTTGCTTCCACATGCGCAGGAACGATCGCGTAATCGGGTTATATCGCGGCGATACGCGCGGGTTATTACGTTCGACCGCATCCAGATACTGGGTCATATCGTTGGAGCCGATCCTGCAGACATCGCCCTCATCGCAGCATTAGTCGATGCTGTCGcac > 2:902025/1‑287 (MQ=255) tACCCGGGCTACTTCTTACCCA‑‑CTCAGCCAGGCCCAGCCCAAGCAGTCGCGGCAGATAACGT‑CTTGCACCGCCAATTTCACCGCAAATGCC‑TACCCATGTGCCCCGCTGGTGCGCAGTGGTAACTATTTGCGGCAACATGCGCAGGAACGATGGCGTAATCGAGTTATATAGCGGCGATACGCGCGGGTTATTACGATCGACCGCATACAGATACTGGGTCATATCGTTGGAGCCGATACTGAAGAAATCGACCTCATCGCAGAAGTGGTCGATGATGTAGCACACCGACGGAACTTccac < 1:443756/301‑1 (MQ=255) ggCTAC‑TCATACTCAG‑CTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGG‑CTTTCACCGCCCAGTTCACCGCAAATGCC‑TACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCGGCGATACGCGCGGGTTATTACGATCGACCGCATACAGATACTCGGTCATATCGTTGGAGCCGATACTGAAGAAATCGACCTCATCGCAGAAGTGGTCGATGATGTAGCACACCGACGGAACTTCCACCATGaa > 2:943698/1‑300 (MQ=255) ggCTAC‑TCATACTCAG‑CTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGG‑CTTTCACCGCCCAGTTCACCGCAAATGCC‑TACCCATGTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACa < 1:947546/132‑1 (MQ=255) | AACAATCTCTTCCCGCTGCCAGACATCTTCTTCCAGCTCAAACGGATGTTCAGTGCGCCCGTTAACGCCGAGGTTGCCGCACAGGAACTGGATCGCCTGCTCTTTATTGCTAAAATCCTGATCAACAAAGATATTCTCCAGCGCCAGCAGTGGGCGAACGTCTTCTTCCGGCGTAAAGGCGGTGAGTAACGCTTCAATTTCCTGCGCACTGCGGCATTCACATGCCTGACGCGCCAGTTCCCGACACGCCTCGCTATCCAGTTGACGAAGCTGGCTTTTCACCG‑CCGGAATACGCGGGCTAC‑TCATACTCAG‑CTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGG‑CTTTCACCGCCCAGTTCACCGCAAATGCC‑TACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCGCAGGAACGATGGCGTAATCGGGTTATATAGCGGCGATACGCGCGGGTTATTACGATCGACCGCATACAGATACTGGGTCATATCGTTGGAGCCGATACTGAAGAAATCGACCTCATCGCAGAAGTGGTCGATGATGTAGCACACCGACGGAACTTCCACCATGAT > NC_000913/2502281‑2502877 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |