Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,097,158	C→T	13.3%	intergenic (+329/+407)	ycdU → / ← serX	putative inner membrane protein/tRNA‑Ser

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,097,158	0	C	T	13.3%	83.5 / 9.0	64	intergenic (+329/+407)	ycdU/serX	putative inner membrane protein/tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base C (27/25);  new base T (5/3);  total (32/32)
Fisher's exact test for biased strand distribution p-value = 7.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.21e-01

TATATGTTCTGTACTCTCTTGCGCATTTTGATTGTTGACTGAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTG‑TG‑CAATA‑TACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCT‑GTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATA‑CACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGG‑‑CAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGC‑TTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTC  >  NC_000913/1096860‑1097455                                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                                              tattgtttCTGTACTTGTTGGCGCATTTTGGTTGTTGAATGAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGGGGGCGGGGGCACCCGGTGTG‑TG‑CAATA‑TACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCT‑GTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATa‑cac                                                                                                                                                                                                                                                                                                             <  1:888561/295‑1 (MQ=255) tatatGTTCTGTACTCTCTTGCGCATTTTGATTGTTGACTGAGTAACGAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTG‑TG‑CAATA‑TACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCT‑GTCGCCAGTTGTCGCCCCCCGGTCGGGGCTTCTCATCCCCCCGGGTTGTGCACTa‑ca                                                                                                                                                                                                                                                                                                              >  2:967879/1‑300 (MQ=255) tatagtttcgtgaCTCCCTTGCGCATTGTCATTGTTGACTGAGTAACCAGACAGTTGATGTGCAAGATTTCCCCGCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTG‑TG‑CAATA‑TACGAAAAAAAAGCCCGTACTTTCGTACGGGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCT‑GTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATa‑c                                                                                                                                                                                                                                                                                                               <  2:399687/287‑1 (MQ=18)                                           ataatcaaggaGTGGAGGTCCACGATTACCCCTCGCCCTAACAGACGTGTGCGTTGGCACCCGGTGGT‑TG‑AAATG‑TACGAAAAAAAATCCCGTACTTTCGTACGAGCGCTTCTTTAAATATGGCGGTGAGGGTGGGATTGACCCGCTTCGCTCGCCCTGCGGGCAGCCCGCGCACTTCGTTCACGGTCTGTCCAACTGGCT‑GACGCCAGTTTTCGACCTCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATA‑CACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAata                                                                                                                                                                                                                                                                   <  2:308423/291‑1 (MQ=14)                                            tAAACACACAGGTGATTGTCACGAGTTCCCCCCGCCCTAAAAGATGTGGGCGGGGGCACCCGG‑GTGTTG‑CAAAA‑TACCAAAAAAAAGCTCGGCCTGTCGGACGAGCTCTTCTTTAAAGATGGCGGTGAGGGGGGGATTGACTCGCGGCGCTCGCCCTTCGTGCAGCCCGCTCACGGCGGTCACGGTCTGTCCAACTGGCG‑GTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATA‑CACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAata                                                                                                                                                                                                                                                                   <  2:1440143/300‑1 (MQ=14)                                                      gTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTG‑TG‑CAATA‑TACGAAAAAAAAGCCCGTACTTTCGTACGCGCTCTTCTTTAACTATGGCGGTAAGGGGGGGATTTACTCGCTTCGCTCGCACTGCGGGCAGCCAGCGCACTACTTTCACGGTCTGTCCACATGGCT‑GTCGCCAGTTGTCGCCCCCCGCTCGTGGCTTCTCATCCGCCCGGTGTGTGCGAAA‑CACGCACAAAAAGACCTTCGTTTAGTACGAGCTCTGCTTTACATCTTGCGATGAgg                                                                                                                                                                                                                                                        >  2:1524299/1‑301 (MQ=17)                                                         ctcggagatgtgtagaagagaaagtaccAGACGTGGGCGGGCGCACCCGGTGGT‑TG‑CAATA‑TACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTGAAATAGGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCT‑GTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATA‑CACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAgggg                                                                                                                                                                                                                                                      <  2:1253229/273‑1 (MQ=11)                                                                 cGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTG‑TG‑CAATA‑TACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCT‑GTCGCCAGGTGTCGACCCCCGGTCGGGGCTTCTCGTCCCCCCGGTGTGGGCAATA‑TACGAAAACAAAACCCGTACTTTCGTACGAGCTCTTCTTTTAATATGACGGAGAGGGGGGGGTTGat                                                                                                                                                                                                                                             >  2:784402/1‑300 (MQ=14)                                                                  taTTCCCCCACTCCCTAATGGATGTGGGGGGGGGAACGCGG‑GGG‑TG‑GAAAACTAGGAAAAAAAAGCCTGTGCTTGAGGACTAGATGGTCCATAAAAGTGGCGGTGAGGGGGGGACTGACTCGCTTCGCTCGCGCTGGGGGAAGCCCGCTCACGGCGTGCACGGTCGGTCCAACTGGCG‑GTCGCTTGTTGTCGACCCCCGGTGGGGGCTTCTCATCCCCCCGGTGTGTGCAATA‑CACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACt                                                                                                                                                                                                                                            <  1:778284/300‑1 (MQ=2)                                                                   aTTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTG‑TG‑CAATA‑TACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCT‑GTCGCCCGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTCTGCCTGT‑CCCGAACAGAATGCCCGTACCGTCGTACGCGCTCTTCTTTAAATATgcctgtcagtgtgcgaggcaca                                                                                                                                                                                                                                            >  1:233208/1‑279 (MQ=14)                                                                   aTTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTG‑TG‑CAATA‑TACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTACGGGCAGCCCGCTCACTGCGGTCACGGTCTGTCCAAATGGCTCGTC‑CCCTTTCTCACCCCCCGCTCCGGCCTCTCCCTCCCCCCCGTGTGTTCAATA‑CCCCAAAGAAAACCCCGTACTTCCGTCCCACCCCGTCCTTCAATTTTGACGGGAGGGGGGGactacctt                                                                                                                                                                                                                                           >  1:359015/1‑294 (MQ=9)                                                                       cccactccacgaaatgcggggtccctgggCATCCGGTGTG‑TC‑CAAAG‑CGCGAAAAAAAAGCCCAACCTTTCGGACGAGCTGTTCTTCAAGGTTGGCTGTGAGGGGCGGATTGACTCCAGTGCCTTCCCCTGCGGGCAGCCCGCTGCCGGCGCTCACGGTCTCTCCTACTGGCT‑GTCGCCATATGTCGACCCCCGGTCGGGGCTTCTCAGCGCCCTGGTGTGTGCAATA‑CACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACtcgctt                                                                                                                                                                                                                                       <  2:1241072/275‑1 (MQ=2)                                                                          cacgactgaaaggACGTGGGGGGGGGCATCCGG‑GGG‑TGAGAAGA‑TAAGAAAAAAAAGCCCGTACTTTGGGACGAGCTGTCCTTGAAATGTGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCT‑GTCGCCAGTTGTCGACCCCCGGTCGGGGGTTCTCATCCCCCCGGTGTGTGCAATA‑CACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGATTGACtcgcttcgc                                                                                                                                                                                                                                    <  2:1311461/289‑1 (MQ=11)                                                                              cccTAACAGACGTGGGCGGGGGCACCCGGTGTG‑TG‑CAATA‑TACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCACGCTCACTGCGTTCACAGTCTGTCCAACTGGCT‑GTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCCATA‑CACGAACAAAAAGACAGTACTTTCGTACGAGCTCCTCTTTAAATATGGCGGTGAagggacgcttacagagattcgctgg                                                                                                                                                                                                                                 >  2:909809/1‑279 (MQ=14)                                                                                 tAACAGACGTGGGCGGGGGCACCCGGTGTG‑TG‑CAATA‑TACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCACCTGGCT‑GTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATA‑CACGAAAAAAAAGCCCGTCCTTTCCTACGCGCTCTTCTTTCACTATGGCAGTggggggctgcctcttctccacagtcgcccc                                                                                                                                                                                                                              >  1:1253192/1‑276 (MQ=14)                                                                                                ggggCACCCGGTGTG‑TG‑CAATA‑TACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTATGTCCAACTGGCT‑GTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATA‑CACGAAAAAAAAGCACGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGG‑‑CAGCCCGCt                                                                                                                                                                                                             >  2:1470906/1‑300 (MQ=25)                                                                                                   gCACCCGGTGTG‑TG‑CAATA‑TACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCCCTGCGTTCACGGTCTGTCCAACTTGCT‑GTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATT‑TACGAAAAAAAAGCCCGTACGTTGGTACGAGCCCTTCTTTACATTAGTCGGTGCAGGGGGGATTGGCacgcttcgtacggtcacgtggccggccgctccc                                                                                                                                                                                                            >  2:397903/1‑275 (MQ=11)                                                                                                    cACCCGGTGTG‑TG‑CAATA‑TACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCT‑GTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATA‑CACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTAAGGGGGGGATTTACCACCTTCACTCGACATGCGGG‑‑CAGCCCGCTccacg                                                                                                                                                                                                        >  2:297069/1‑297 (MQ=255)                                                                                                    cACCCGGTGTG‑TG‑CAATA‑TACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCT‑GTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATA‑CACGAAAAAAAAGAACGAACTTTCGTACGAGATATTCTTTAAATATGCAGGGGAGGGGGGGATTGACACGCTTCGATCGCCATGCGGG‑‑ACGCCAGCGCACtt                                                                                                                                                                                                        >  2:1318090/1‑300 (MQ=11)                                                                                                    agataggTGTG‑GT‑CAAGA‑TGCGAGAAAAACGTCCTGACGGTCGTACCAGGTCTTCGTGCAAGTGGGCGGTGAGGGGGGGAGTGCCTCGCTTCGCTCGCCCTGAGGGCAGCCCTCGCCTGGTGTACCTGGTGCGTCCAATCGGTT‑TTGTCCATTTGTCGACCCCCTGTCGGTTTTTTTCAACCCTCCGGTGTGTGCAATA‑AACGAAAAAAAAGCCCGTACTTTAGTACGAGCTCTTCGTTAAATATGGCGGTGAGGGGGGGATTGACTCGTTTCGCTCGCTCTGCGGG‑‑CAGCCCGCTCAc                                                                                                                                                                                                          <  2:1367313/294‑1 (MQ=2)                                                                                                        cccactttagtgcactccatgAAAATAAGGCCTGTCCTTTCGTCCCAGTTTTTCTTTCATGTTGCCTCAGAGGGGGGTACAGCCTTTCTGCGCTCGCCCCTCGGGCACCCCGCTGCCGTCGTTCACGTGTTATCCAAGTGGCC‑GTCGCCAGTTGTCGACCCCCGGTCTGGCCTTCTCATCCCCCCGGTGTGTGCAATA‑TACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGG‑‑CAGCCCGCTCACTGc                                                                                                                                                                                                       <  2:514850/281‑1 (MQ=2)                                                                                                          gtgtg‑tG‑CAATA‑TACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCACTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCT‑GTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATA‑CACGAACCAAAAGACCGTACTTTCTCCCGAGTCATTCTTTCAATCTGGCGGTGAGGGGGGGATTGACTCGTTTCCATCGCCGCGCGGG‑‑CACCGCGCTCCCACCGTTc                                                                                                                                                                                                   >  2:762950/1‑300 (MQ=11)                                                                                                                 g‑CAATA‑TACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCT‑GTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGCGTGCCCTA‑CACCACAAAAAACCCCGTACTTTCGTACGAGCTCTTCTTTCAATATGGCGGCCGGGGGGGGATTGCCCCGCTTCGATCGCCCTGCGGG‑‑CCACCCTCCCACAGCCTTCAAggac                                                                                                                                                                                             >  1:1135442/1‑298 (MQ=11)                                                                                                                               aaaaaaGCCCTGTCTGTCGGAGGGGCTTTTTTTTAAATTTGGCGGTGGGGGGGGGATTGACTCGCTGCGCGCGCACGGGGGGCAGCCCGTTCACTGCGTTCCCGGCTGGTCCAACGTGCT‑TCCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCCTCCCCCCGGTGTGTGCAATA‑CACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGG‑‑CAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCt                                                                                                                                                                                <  2:359022/301‑1 (MQ=11)                                                                                                                                                            tcttTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCT‑GTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTTCACTA‑CCCGAACAAAAAGCACGTACtt                                                                                                                                                                                                                                                                                          >  1:1255449/1‑168 (MQ=255)                                                                                                                                                            tctgTAAATATGGCGGTTAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCT‑GTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATA‑CACGAAAAAAAAGCCCGTACtt                                                                                                                                                                                                                                                                                          <  2:1255486/168‑1 (MQ=18)                                                                                                                                                            tcgtgaaagcgggCGGTTAGCGGGGGATTGACGCGCTTCGCTTGCCTGGCGGGCAGCCAGCTCACGGCGTTCACGGTCTGTCCAACTGGCT‑GTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCTGTGTGTGCAATA‑CACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGG‑‑CAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGc                                                                                                                                                   <  2:1418159/290‑1 (MQ=11)                                                                                                                                                                      tgggggggggggggggATGGACTCGCTTGGCGCGCCCTGCGGGCAGCCCGCTCACTGCGTTCATGGTCTGTCCAACTGGCT‑GGCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGAAATA‑CACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGG‑‑CAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGC‑TTCTCATc                                                                                                                                          <  2:147951/290‑1 (MQ=255)                                                                                                                                                                            gtaGGGGGGGATTGACGCGCTCCGCCCGCCCTGCGGGCAGCCCGCTCATGGCGTTCACGGTTTGTCCATCGGGCG‑GTCGCCAGTTGTCGACACCCGGTCGGGGCCTCTCATGCACCCGGTGTGTGCAATA‑CACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGG‑‑CAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGC‑TTCTCATCCCCCCgg                                                                                                                                   <  2:233212/298‑1 (MQ=11)                                                                                                                                                                              gggggggggTCGACGCGCTGCGCTCGCCCTGGGGGCAGGCCTCCGACTGCCTTCCCGGTCTGCCCAACTGGCT‑GTCGCGGGGTGGCGAGCCCCGTTGGGGGCGTCTCGGCCCCCCGGTGTGTGCAATA‑CACGAAAAAAAAGCCCGTACTTTCGTACGGGCTCGTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGG‑‑CAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGC‑TTCTCATCCCCCCGgtg                                                                                                                                 <  1:1470862/299‑1 (MQ=11)                                                                                                                                                                              aGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCT‑GTGGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGGGTGTGCAATA‑CACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGG‑‑CAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGC‑TTCTCATCCCCCCGgtg                                                                                                                                 <  2:1135473/300‑1 (MQ=17)                                                                                                                                                                                       ttgcgatccttctcctgcccgtgtgcaaGCCCCCTCACTGCGCTCGCGTGCTTTCCCACTGGGC‑GTGGCACGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCGGGTGTGTGCAATA‑CACGAAAAAAAAGCCCGTACTTTCGGACGAGCTCTTCTTTAAACAGGGGGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGG‑‑CAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGC‑TTCTCATCCCCCCGGTGTGTGCAatat                                                                                                                       <  1:1316390/274‑1 (MQ=11)                                                                                                                                                                                       ttgcctcgtgtgccccccccggggggCCGCCCCGTCACTGCGATCACGGGCGTTCCGACGGGCT‑GTCCCCAGGTGTCGACCCCCGGTCGGTGCTTCTCTTCCCCCCGGTGTGTGCAATA‑CACGAAAAAAAAGCCCGTACTTTCGTACGGGCTCTTCTTTAAACAGGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGG‑‑CAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGC‑TTCTCATCCCCCCGGTGTGTGCAatat                                                                                                                       <  2:442259/278‑1 (MQ=11)                                                                                                                                                                                         gACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCT‑GTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATA‑CACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGG‑‑CAGCCCGCTCACTGCGTTCACGGCCTGTCCAACTGTCTGTCGCCCGTTGTCGACCCCCGGTGGGGGC‑TTCTCATCCCCCCGGGTTGTTCAAtata                                                                                                                      >  2:915227/1‑300 (MQ=255)                                                                                                                                                                                            ccgcttcgccccgctcgCGGGCATGCCGGTAAGTGCGTGCACGGCTGGTCCATCGGGCG‑TTCGCCATTTGTCCGCCCCCGGTCGTGGCGTCTCATCCCCCCGGTGTGTGCAATA‑CACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGG‑‑CAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGC‑TTCTCATCCCCCCGGTGTGTGCAATATACGaa                                                                                                                  <  2:620622/285‑1 (MQ=11)                                                                                                                                                                                                 tcgctcgcCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCT‑GTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATA‑CACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTCAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGG‑‑CAGCCCGCTCACTGCTGTCACGGTCGTTACACCTGGCTGTCGCCAGGTGTCGAACCCCGCACGTGGA‑ACCTCATCACCCAGGTGCGTTCGatatcattatccta                                                                                                             >  2:1316430/1‑291 (MQ=11)                                                                                                                                                                                                      cgcCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCT‑GTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATA‑CACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGG‑‑CAGCCCGCTCACTGCGTTCACGGTCTGTCCACCTGGCTGTCGCCCGTTGTCGATCCCCGGTCGGGGC‑TTCTCCTCCCCCCGGTGTGTGCAATATCAGAACAAAACGcc                                                                                                         >  2:975393/1‑300 (MQ=255)                                                                                                                                                                                                       gcCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCT‑GTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATC‑CACGAAAAAAACGCCGCTACCGTCGTCCGCGCTTTGATTTATATATGGCGCGGAGGGGGTGGTTCCCTCACTTCACTCGCCCTCCCGGCCCAGCAC‑CTCCCT‑CGTTCCGCGTCCTGCCCACTGGCTGTCCCCCGTTGCCTGCCCCCCGTCGGGCC‑TTCCTACACCCCCCGTGTGTGCacacttccacccaacagcca                                                                                                        >  1:775428/1‑281 (MQ=2)                                                                                                                                                                                                                          cACTGCGTTCACGGTCTGTCCAACTGGCT‑GTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATA‑CACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGG‑‑CAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCTCCAGTTGTCGAACCCCGGTCGGGGC‑TTCTCATcccc                                                                                                                                       >  2:939731/1‑250 (MQ=17)                                                                                                                                                                                                                                 ttCACGGTCTGTCCAACTGGCT‑GTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATA‑CACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGCGGGGGGGATTGACTCGCTTCTCTCGCCCTGCGGA‑‑CACCCCGCTCACTGCGTTCCCGGTCTGTCCCACTGGCTGTCGcc                                                                                                                                                                          >  2:607238/1‑209 (MQ=255)                                                                                                                                                                                                                                 ttCACGGTCTGTCCAACTGGCT‑GTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATA‑CACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGG‑‑CAGCCCGCTCCCTGCGTTCACTGTCTGTCCACCTCGCTGTCGCCCGTTGTCGACCCCCGGTCGGGGC‑TTCTCATCCCCCCGGTGTGTGCATTTTTCTACACAAAAGCCCGTACGTTCGTACGAGCTCTTCtttcca                                                                             >  2:722447/1‑298 (MQ=11)                                                                                                                                                                                                                                  tgaCGGTCTGTCCACCTGCCT‑GGGCCCAGTGGGCGCCCCCCGGTGGGGTCTTCCCCTCCCCCGGGTGTGTGAAAGA‑CCCGAAAAAAGAGCCCGTACTTTCGTACGAGCTTTTCGTTAAATATGGCGGTGAGGGGGGGGTTGCCTCGCTTCGGCCGCCCTGCGGG‑‑CAGCCCGCTCACTCCGTTCACGGTCTGTCCACCTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGC‑TTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAat                                                                            <  1:722438/299‑1 (MQ=11)                                                                                                                                                                                                                                  tCACGGGCTGTCCAACGGGCG‑GTCGCCAGTTGACAACACCCGGAGGGGGCTTGCCATCCCCCGGGGTGGTGCAAAT‑AACGAAAAAAAAGCGCTGGCGTTCGTACGAGGTCGTCTGTAAATATGGCGGGGAGGGGGGGATTGACGCGCTTCGCACGCCCTGCGGG‑‑CAGTCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGC‑TTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAat                                                                            <  1:977178/301‑1 (MQ=11)                                                                                                                                                                                                                                  ccagggTCTGCCAAACTGGCT‑TACGGCAATTTGCGACCCGCGTTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATA‑CACGAAAAAAAAGCCCGTACTTTCGTACGGGCTCTTCTTTAAATATGGCGGGGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGG‑‑CAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGC‑TTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAat                                                                            <  2:500007/297‑1 (MQ=11)                                                                                                                                                                                                                                   acggatcttgtcaaacgctcgacccgcatttGTCGACCCCCGGGCGGGCCTACTCTTCCCACCTGTGAGTGCAAAG‑CAGGAAAAAAAAGCCCTTACTATCGTACGGTCTCTGCTTTAAATATGGCGGTGGGGGGGTGATTGACTCGCTTCGCTCGCCCTGCGGG‑‑CAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGC‑TTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTGCGTACGAGCTCTTCTTTAAat                                                                            <  2:42883/272‑1 (MQ=11)                                                                                                                                                                                                                                    gtgctgggagagtgggataacgggcgtgTTATCGACCCCCTGTCGGGTGTTCTGCGCCCCCCGGGGTGTG‑AATACCACGAAAAAAAAGCCCGTCCTTTCGTACGATCGCTTCTTTAAGTTTGTGGGTGGGGGGGGGATTGCCTCGCTGCGTGCGCCCTGCGGG‑‑CAGCCCGCTCACTTCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGT‑TTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAat                                                                            <  2:754438/273‑1 (MQ=11)                                                                                                                                                                                                                                           gTCCAACTGGCT‑GTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATA‑CACGAAAAAAAAGCCAGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGATTGAATCGCTTCGCTCGGCCTGAGGG‑‑CAGCCAGCTCCCTCCGTTCACGTGCTGTCCACCTGGCCGTCGCCCGGTGTTGAACCCCGGTCGGGGG‑TTCTAAGCCCCCCGGGGTGTGCAATCTACGAACAAAAACACCGTACTTTAGTACGAGACATTCCTTAAattttcacg                                                                     >  2:1193585/1‑292 (MQ=11)                                                                                                                                                                                                                                                               gTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATA‑CACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGG‑‑CAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGC‑TTCTCATCCCCCCGgtg                                                                                                                                 <  2:1060568/220‑1 (MQ=32)                                                                                                                                                                                                                                                                  gTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATA‑CACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGG‑‑CAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTTTCGCCAGTTGTCGAACCCCGGTCGGGGC‑TTCTCATCCCCCCGGTTTTTGCCATTACCCACAAAAAAGCCCGCACTTTCCTACGAGCCCTTTTTTACAATTTCTGTTTA‑GGGGGGGATTTACtcgcttc                                             >  2:496406/1‑300 (MQ=11)                                                                                                                                                                                                                                                                    cacaccccGGTCGGGTCCTCTCATCCCCCCGGGGTGTCAAACA‑TACGAAAAAAAAGTCCGTACTGTCGTACGGGCTTCTCTTTAACTGTGGCGGGGAGGGGGGGATTGCCTCGCTTCGCTCGGCCTGGGGG‑‑CGGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGC‑TTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTGACtcgcttcgct                                          <  2:452338/297‑1 (MQ=11)                                                                                                                                                                                                                                                                        tcggcctgtggcctcttttatccccgGGTGTGCCC‑ATA‑TACGAAAAAAAACCCCGTACTGGCGTCCTAGGCATTCTTGAAAGCTGTGGGGGAGGGGTGGCTGTCCTCGCTTCGCTGCCCCTCGGGG‑‑CAGCCCTCTCTCTGCGTTCACGGGCTGTCCACCGGGCTTTCGACATTTTTAG‑ACGCCGGTTGGGGCAGTTTCGTACCCGCGGCTTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTGACTCGCTTCGCTCGccc                                     <  1:1303041/280‑1 (MQ=0)                                                                                                                                                                                                                                                                          ccGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATA‑CACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGG‑‑CAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGC‑TTCTACTCCCCCCGGTGTGTGCCATATACGAAAAAAAAGCCCGTACTTTCTTCCGAGCTCTTCTTTCAat                                                                            >  2:1017464/1‑262 (MQ=9)                                                                                                                                                                                                                                                                             gTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATA‑CACGAAAACAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAgggggg                                                                                                                                                                                                                                                    >  1:132926/1‑94 (MQ=18)                                                                                                                                                                                                                                                                             gTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATA‑CACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAgggggg                                                                                                                                                                                                                                                    <  2:132930/94‑1 (MQ=33)                                                                                                                                                                                                                                                                                 tgactgctcATCCCCCCGGTGTGTGCAATA‑CATGAAAAAAAAGCCCGTACGTGCGTGCGAGCTCTTCTTTAAATATGGGGGTGGGGGGGGGATTGACTCGCTGCGCTCGCCCTGCGGG‑‑CAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGC‑TTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGc                              <  2:29797/294‑1 (MQ=255)                                                                                                                                                                                                                                                                                   gCTTCTCATCCCCCCGGTGTGTGCAATA‑TACGAAAAAACAGCCCGTACTTTCGTACGCGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGG‑‑CAGCCCGCTCACTGAGTTCACGGTCTGTCCACCTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGC‑TTCTCATCCCCCCGGTGTGTTCACTCACCCACAAACAAGCCCCCACTTTCCTACGAGCACTTCTTTCACCCGGCCGGTGC‑AGTGGTGCATGACTCGCTCTGCTCACCCTGCggcacg                            >  2:341978/1‑296 (MQ=11)                                                                                                                                                                                                                                                                                      tctcATCCCCCCGGTGTGTGCAATA‑CACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGG‑‑CAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGCCTGTCGCCAGTTGTCGCACCCCGGGCGGGGA‑TGCCCATCCCCCCGGTGTGTGCCATATACGAAAAAAAAACCCGGACATTCTTACGATCTCTTCTTCACATATGGGGGTGA‑GGGGGGGGTTGACtgccctggctgcgcctggggggcggcc                         >  1:325939/1‑274 (MQ=11)                                                                                                                                                                                                                                                                                         cATCCCCCCGGTGTGTGCAATA‑CACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGG‑‑CAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGC‑TTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTGACTCGCTTCCCTCGCCCTGCGGGCAGCCCGCt                      >  2:35719/1‑300 (MQ=25)                                                                                                                                                                                                                                                                                           tCCCCCCGGTGTGTGCAATA‑TACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGG‑‑AAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGC‑GTCTCATCCCCACGGTGTGTGCCATATAGGCAAAGCAAGCCCGTACTTTCGTACGACGTGTTCTTTGAATAGTGCGGTGA‑GGGGGGGATTCACtagcacccgccgcgcaccgtgcaacccgcgca                    >  2:1353904/1‑272 (MQ=11)                                                                                                                                                                                                                                                                                             cccccGGTGTGTGCAATA‑TACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCACTGCGGG‑‑CAGCCCGCTCACTGCGGTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGC‑TTCTCATCCCCCCGGTGTGTGCAATATACGAAAACAACGCCCGTACTTTGGTACGAGCTCTTCTTTTGATATGGCGGGGA‑GGGGGGGGTTAACtggattccgttgagctgagggacgcccgccca                    >  2:954659/1‑273 (MQ=11)                                                                                                                                                                                                                                                                                             cccccGGTGTGTGCAATA‑CACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTTACTCGCTTCGCTCGCCCTGCGGG‑‑CCGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGC‑TTCTCATCCCCCCGGTGTGTGCAATATACGCAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATTTGACGGTGA‑GGGGGGGATTTACTCCCTTTGCCCGCACCGCgggacgacgggcact                   >  2:445930/1‑287 (MQ=11)                                                                                                                                                                                                                                                                                             cccccGGTGTGGGCAATA‑CACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGG‑‑CAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGt                                                                                                                                                                       <  2:1244296/153‑1 (MQ=14)                                                                                                                                                                                                                                                                                                       gtgCAATA‑CACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGG‑‑CAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGC‑TTCTCCTCCCCCCGGTGTGTGCAATCTACGAAAAAAAAGCCCGTACTTTCGTACTAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCCCACTGCGTTCACgg        >  2:415960/1‑301 (MQ=12)                                                                                                                                                                                                                                                                                                       gtgCAATA‑CACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGG‑‑CAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGC‑TTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGTTGACTAGCTTCGCTCGCCCGGCGCGCAGCCCGCGCACTCCGTTCACGGt       >  2:446713/1‑301 (MQ=12)                                                                                                                                                                                                                                                                                                        tgCAATA‑GACAAAAAAAAAGCGCGTACTGTCGGACGATCTCTTCTTTAAATATGGCGGTGAGTGGGGGTTTGCCTCGCTTCGCTCGCCCTGCTGG‑‑CAGCCCGCTCACGTCGTTCACGGTCTGTCCATCTGGCGGTCGCCAGTTGTCGAACACCGGTCGGGGC‑TGCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTc      <  2:1267762/301‑1 (MQ=11)                                                                                                                                                                                                                                                                                                         gCAAGA‑GACGAAAAAAAGTCACGGACTTGCGTACGGGCGCTGCTTTAAATATGGCGGGGAGGGGGGGATTGAGTCGCTTCGCTCGCCCTGCGGG‑‑CAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGC‑TGCTCATCCCACCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTc      <  2:592721/300‑1 (MQ=11)                                                                                                                                                                                                                                                                                                            aTA‑CACGAAAAAAAAGCCCGTACTTTCGGACGCGCTCTTCTTTAAATTGTGCGGTGGGGGGGGTATTGACGCGCTTCGCTCGCCCTGCGGG‑‑CAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGC‑TTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTc  <  2:325944/301‑1 (MQ=255)                                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                                              TATATGTTCTGTACTCTCTTGCGCATTTTGATTGTTGACTGAGTAACCAGACAGTTGATGTGCACGATTTCCCCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTG‑TG‑CAATA‑TACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCT‑GTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATA‑CACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGG‑‑CAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGC‑TTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTC  >  NC_000913/1096860‑1097455

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑