| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,171,897 | A→G | 17.9% | intergenic (+141/‑160) | rrfB → / → murB | 5S ribosomal RNA of rrnB operon/UDP‑N‑acetylenolpyruvoylglucosamine reductase, FAD‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,171,897 | 0 | A | G | 17.9% | 266.3 / 60.9 | 167 | intergenic (+141/‑160) | rrfB/murB | 5S ribosomal RNA of rrnB operon/UDP‑N‑acetylenolpyruvoylglucosamine reductase, FAD‑binding |
| Reads supporting (aligned to +/- strand): ref base A (58/73); new base G (15/15); total (77/90) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.85e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.09e-01 | |||||||||||
Only 100 of 167 total aligned reads displayed.
ACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑AC‑GCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAA‑CCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCAT‑AATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAACCGCAGAAGGACAACCCGTTCTTATTCTGG > NC_000913/4171608‑4172195 | cGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATACACCGAACGGCTCAGTCGAAAGACTGTGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGCATTTGAACGTTTCGCAGCAACGGCCCGGCGGGCGGCGGCCAGG‑Gc‑ggccgcc > 1:1140020/1‑300 (MQ=11) cGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGACGCAACGTCCCGGCGGTCGGCGGGCAGG‑Ac‑gcccgcc > 2:879622/1‑300 (MQ=12) aagggacaggATAAAAAAGAATGTGCGTGGCGGCGGTGGGGCGGTGGTCCCACCTGACCCCATGCCGAACGCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGGGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑AC‑GCCCGCCATAAAc < 1:1341005/292‑1 (MQ=11) aaGCGGTTGGATAAAACAGGATGTGCCTGGCGGCAGGAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑AC‑GCCCGCCATAAAc < 2:142354/301‑1 (MQ=14) gATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGGGGCGGGCAGG‑AC‑GCCCGCCCTCAAc > 2:1341045/1‑292 (MQ=17) aaTTGGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCAGGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑AC‑GCCCGCCATAAACTGCCAGGCATCAAAt < 2:337012/297‑1 (MQ=17) attggcctcggtgcagtatcggtgtgtgcacACCTGCGCCCAGGCCGAACTAATAAGGAAAACGCCGGAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACGGGGCCTTTCGTTTTATCTGTTTTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑AC‑GCCCGCCATAAACTGCCAGGCATCAAATTAAGc < 2:816900/271‑1 (MQ=11) aTTGGCCGGGCGGCAGGAGCGCGGTGTTCCCACCGGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGGCGATGGTAGGGGGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑AC‑GCCCGCCATAAACTGCCAGGCATCAAATTAAGc < 2:1367466/301‑1 (MQ=9) gtGCCTGGCGGCAGGAGAGGGGTGGGCCAACCTGACCCCATGCCGAACGCAGAAGTGAAACGCCGGAGCGCCGATGGGATGGTGGGGCCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCGCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCGGG‑AC‑GCCCGCCATAAACTGCCAGGCATCAAATTAAGc < 2:457743/298‑1 (MQ=11) tGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCGAATAAAACGACAGGCTCAGTCGAAAGACTGGGCATTTCGTTTTATCTGTTGTTTGTCGGTCAACGCTCTCCTGAGGCGCACAAATCTGCCGGGAGCGGAGTTTAACGTTGCGATGCAACGGACCCGGGGCGAGCGGGCAGG‑GC‑GCCCGCCATaacaggcacgggcgcaaaatcaccaga > 1:1186747/1‑276 (MQ=11) agggtgccggggggtCCCAACCGACCCACTGCCGAACTCAGAAGTGAACCGCCGTAGCGCCGATGGTAGTGTGGGTTCTCCACATGCGATAGTAGGAAACTGCCAGGAATCAAATAAAACGAAAGGCGCAGCCGAAAGACGGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑AC‑GCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATcc < 1:426156/288‑1 (MQ=11) gTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑AC‑GCCCGCCATAAACTCCCAGGCCTCAAATTAAGCAGAAGGcacaccgg > 1:661034/1‑293 (MQ=17) gTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATGTGAACGTTGCGAAGCCACGGCCCGGAGGGCGGCGGGCAGG‑CA‑GCACCACCTCAACCGCCCGGCACAACCTTAAGCAGACGGCCCACCTg > 1:252391/1‑300 (MQ=11) gggcgGGGGGCCCACCTGACCGCAGGCCGAACTCAGAAGTGAAACGGCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGAATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑AC‑GCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACg < 1:206007/298‑1 (MQ=12) cgGTGGTCCCACCTGACCCCATGTCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTTAGTCGAAAGACTGGGCCATTCGTCTTATCAGTTGTTTGTCGGTAAAAGCTAACCTTAGTAGGACAAATCCCACGGGAGCGGACTTGAACGTTGCGAAGCAACGGTACGGAGGGTGGCGGTTGGG‑AC‑GCTCCCCAGACTCTGTAAGACACCGATTTACGCAGAAGGCAATCCGGACGAATg > 2:981904/1‑301 (MQ=11) ggtCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGAGTGGGGTCTCCCCATGCGCGAGTAGGGAACTGCCAGGCATCACATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGCTTTGAACCTTGCGAAGCACCGGCCCGGAGGGGGGCGGGCAGG‑AC‑GCCCGCCGACCACTGCCCGGCATCAAATTAAGCAGAAGGCCACCCTAACGGCTGccc > 1:1019323/1‑300 (MQ=11) cacccacgggccgcacgtgcGAACGCAGAAGTGAAATGCCGTGGCGCCGAGGGTCGGTGGGGGTGGCCCCAGGAGGGAGGAGGGATCGGCCAGGGAGCAAATAAAACGAAAGGCTCAGGATAAAGACTGGGCCTTGCGTGTTATCTGTTGTTTGTCGGTGAAGGCTCTCCTGAGGAGGACAGATCCGCGGGGATGGGAGTTGAACGTTGCGAAGCAAATGCACGGAGGGTGGCGGGACGG‑AC‑GCCCGCCATAAACGGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCtt < 1:1023213/282‑1 (MQ=2) cacggACCCCTGGCCGAACTCAGAAGTGGAACGCCGTAGCGCCGATGGTAGGGGGGGGTCTCCCCAGGCGAGAGTAGGGAAGTGCCAGGCATCAAATAAAACGAAGGGCGCGGGCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGGGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑AC‑GCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGc < 1:464226/297‑1 (MQ=11) aCCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCGGG‑AC‑GCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGCTGGCCTTTTTg > 2:1001827/1‑300 (MQ=17) aCCTGACCCCATGCCGAACTCAGAAGTGAAACGACGTAGCGCCGATGGTAGTGTCGGGTCTCCCCATGCGATAGTAGGGAACTGCCAGGCATCACATACAACGAAAGGCACAGTCGACAGACTGGGCCTTTCGTACTCTCTCTTGTTTGTCGGTAACCGCTCTCCTGCGTAGAGCAAATCCGCCGGGAGCGGATCTGAACGATGCAAAGCACAGGCCCTGAGGGTGCCGGGCAGG‑GC‑GCCCGCCCTACACTGCGAGGCCTCAAATTACGCAGACGGCCATCATTGCGGAGAGCCttttgag > 2:341347/1‑298 (MQ=11) ccTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑AC‑GCCCGCCATAAACTGCCAGGCCGCAAATTACGCAGAAGGCCACACTGCAGGATGTACTTTTTGCg > 1:358417/1‑301 (MQ=255) aCCCCATGCCGAACTCGGAAGTGAACCGCCGTGGCGCGGATGGTAGTGTGGGGTCTCCCCATACGAGAGTAGGGAACTGCCAGGCATCAAATAAAGCGAACTGGTCAGGCGAAAGACTGGGCCTTTCGTTTTATCTGTGGTTGGTCGGTGAACGCTCTCCTGAGTAGGAAAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑AC‑GCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTc < 2:1186780/301‑1 (MQ=11) cgaaaacatgagggAAACGCCGGAGCGCGGGTGGGAGTGTAGGGACTACCAATGCGAGAGAAGGAAGGTGCAAGGAGGCAAAGAAAACGAAAGGCTTAGTCGAAAGCCGGGGCCTTTCGTTTTAGCTGTTGTGTGTCGGGGAAGGCTCTCCTGGGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTTCGAAGCAACGGCCCGGAGGGTGGCGGGCGGG‑GC‑GCCCGCCATAAACTGCCAGTCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAActc < 2:924091/288‑1 (MQ=11) aCTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑AC‑GCCCGCCATCAACTGCCAGGCATCAAATTCAGCAGCAGGCCATCCTCACGGATGCCCTTTTTGCGTTTCTACAAACTCtt > 1:265865/1‑300 (MQ=255) aCTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑AC‑GCCCGCCATAAAc > 2:1020483/1‑233 (MQ=32) aCTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑AC‑GCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTCCAAAATCtt > 2:285108/1‑300 (MQ=25) cTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGCGGGTGGCGGGCGGG‑AC‑GCCCGCCATAAACTGCCAGGCCTCAAATTAAGCAGAAGGCCCTCCTGCCGGACGGCCTTTTTGCGTTTCTACACACTCTTc > 1:956870/1‑300 (MQ=255) cataaGTGAGACCCCGTACGTGCGATGGTAGTGTGGGGTAGCCCCATGCGAGAGTAGGGAACGGACAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCGCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑AC‑GCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTg < 2:830945/298‑1 (MQ=14) aaGTGAAACGCCGTACGGCCGAGGGTAGGTGGGGGTCTCCCCAGGCGAGAGTAGGGAACGGCCAGGCATAAAATAAAACGAAAGGCCCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGGGGGTTGTCGGGGAACGCTCTCCGGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑AC‑GCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTg < 2:1041350/298‑1 (MQ=14) gccgcgggggcgagtggcgtgtGGGGGTTCCGCATGTGATGGTGGGGATCGGCCAAGGAACAAATAAAAGGAAAGGCTCGGCCGAACGACTGGGCCTTTAGTTTGATCGGTTGTTTGTCGGGGAACGCTCTCCTGAGTAGGACAAGTCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCAGGAGGGTGGCGGGCAGG‑AC‑GCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATc < 1:494556/281‑1 (MQ=14) ccGTAGCGCGGATGGTAGTGGGGGGTCTCCCAATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAACCGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGACCGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑AC‑GCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTAc < 2:235937/301‑1 (MQ=255) ccGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGCGTAGGACAAATCCGCCGGGCGCGGCTTTGAACGTTGCGAAGCACCGGCCCGGAGGGTGTCGGGCAGG‑AC‑GCCCGCCGTAAACTGCCCGGCATCAAATTAAGCAGCAGGCCATCCTGCCGGCTGGCCTTTTTGCCTTTCTCCAAACTCTTCCTGTAGTCattcctc > 1:390119/1‑295 (MQ=14) ccGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑GC‑GCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGAGGGCCTTTTCGCGTTTCTACAAACCTTTCCTGTCCTCATATCTa > 1:322895/1‑300 (MQ=255) aGCGCCGATGGTGGGGTGGGGTCTCCCCATGCGAGGGTAGGGAACTGCAAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTGGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑AC‑GCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAg < 1:685907/300‑1 (MQ=255) cgcgGAGGGGAGGGTGGGGTCTCCCCATGCGAGGGTGGGGAACGGCCAGGCATAAAATAAAACGAAAGGCGCAGTCGAAAGACTGGGCCTTTCGTGTTATCGGTTGTTTGTCGGTGAACGGTCTCCTGAGTAGGACAAAGCCGCCGGGAGCGGATGTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCGGG‑AC‑GCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGcc < 1:1001800/300‑1 (MQ=14) ccccagtcgacgtggGGGGTCACCCCATGAGAGATTAGGTAACGGCAATTCATCAAATAAAGCAAAAGGCTCAGTTCAAAGACTGGTCCTTTGGTTTTATCTGTGGTTTGTCGGTGAACGCGCACGTGAGGAGGACAAATCTGCCGGGAACGGATTGGAACGTTGCGAAGCAACGTCCCGGAGTGTGGCGGGCGGT‑AC‑GCCCGCCATAAACTCCCAGGCAGCAAATTAATCAGAAGGCCATCCTGACGTATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAg < 2:536197/287‑1 (MQ=255) tGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGCCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCCGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGTGTGTGGCGGGCGGG‑AC‑GCCCGCCTTAAACTGCCAGGCATCAAATTAAGCAGAAGGACCTCCTGACGGATGGCCTTTTTGGGCTTCTACCAACACTTACTTTCGTGATATCTACAAGCCAcccac > 2:566887/1‑298 (MQ=14) gTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAACACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGTCAACTCCGCCGGGCGCGGATTTGAACGTTGCGAAGCACCGGCCCGGAGGGTGGCGGGCAGG‑AC‑GCCCCCCATAAACTGCCAGCCATCACATTAAGAAGCAGCCCCTCCTGACGGACGGCCCTTTTTCCTTGCTACAACCCCTTCCTGTCAACATACCTACAAGCCATaccccc > 1:1103069/1‑300 (MQ=14) gTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGCACGTTGCGAAGCAACGGCCCGGCGGGTGGCGGGCAGG‑AC‑GCCCGCCATAAACTGCCCGGCCTCAACTTAACCAGAAGGCCCTCCTGACGGATGCCCTCTTTGCTTTTATACCCACTCTTCATGCCGTCATTTCTCCAACCCATcccccc > 1:454379/1‑300 (MQ=14) aGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑AC‑GCTCGACATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCAACCTGACGGATGGCCTTTTTGCTATTCTACAAAATCTTCCttccacaatcttcaccaacactccccccaaa > 2:554350/1‑271 (MQ=11) gggggtgccccctgcggaaggtgggACCGGCCAGGCCTCAAAGAAAACTAAGGGTCTAGTTGAATGACTGGGGTTTTCGTTTTATCTGGTGTTTGTCGGTGAAGGCCGTCTGGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGTTGGCGGGCAGG‑AC‑GCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATa < 1:1509723/278‑1 (MQ=17) ctcCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGCGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCCGG‑AT‑GCCCGCCATAAACTGCCCGGCATCAAATTAAGCAGAAGGc > 2:1077397/1‑218 (MQ=12) ccccATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCGGG‑AC‑GCCCGCCATCAACTGCCAGCCCTCAAATTAAGCAGCAGGCCCTCCTGACGGCTGGCCTTTTTGCGTTTCTACCAACTCTTCCTGTCGTCCTCTCTACACGCCACCCCCCCACCGCTACGGttca > 1:92060/1‑297 (MQ=14) ccccATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCGGG‑AC‑GCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTaaa > 2:251283/1‑300 (MQ=255) ccaatgggggagTAGGTAACGGCCAGGCAGCAAAAAAACCTAAAGGTGCAGTGGAAAGAGTGGGTCGTGAGTTTTATCTGTTGTTTGTGGGGGAACGCTCTCCTGAGGAGGACAAATCCGCCGGGAGCGGATTTGAACGTGGCGAAGCAACGGCCCGGAGGGTGGCGGGCGGG‑AC‑GCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACt < 1:625175/292‑1 (MQ=17) gTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCACCGGCCCGGAGGGTGGCGGGCAGG‑AC‑GCCCGCCATAAACTGCCAGGCATCACATTAAGCAGAACGCCATCCTGACGGATGGCCTGTTTGAGCTTCTACAAACTCTTCCTGTCGTCCGATCTACCAGCCCTCCCCCCACAGATAAGGTAAACTAGCCTCGtttt > 2:1168387/1‑301 (MQ=17) gTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTGTATCTGTTGTTTGTCGGTGAACGATCTACTGAGTAGGACAAATCCGACGGGAGCGGATTTGAACGTTGCGAAGGAACGGGCCGGAGGGTGGCGGGCCGG‑AG‑GCGCGCCATAACCTGCGCGGCAACAAATTAAGCAGAAGGCAAGACGGACGGATGGCCTATTGACGATTGTAAAAACTCATACGGTCGTCATATGTGAAAGCAAGCATCTCAACGATACGTTAAACTACACTCTtttt > 2:633992/1‑301 (MQ=2) cTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑AC‑GCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGCCGGATGGCCTTTTTTCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCCACCCCCCACAGATACGGTAAACTAGCCTCTTTTTTGCATCAg > 1:1343044/1‑301 (MQ=255) aaTCAAATAAAACGAGAGGCTCAGTCTAACGTCTGGGCCTTTCTTTTTATCTGTTGTGTGTCGGTGAACTCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGTGCAGG‑AC‑GCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAagcag < 2:1254751/300‑1 (MQ=255) aaTAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑AC‑GCCCGCCATAAACTGCCAGGCATCAAATTACGCAGCAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATCTCTACACGCCCTCCCCCCCCATACACCGTCAACTAACCCCGTTTTTTCAtacgaaaaccaccccta > 1:1081522/1‑285 (MQ=17) aaTAAAACGAAAGGAGCAGTCGAAAGACTGGGCCTTTCGTTTGATCTGGTGTTGGTCGGTGAACGCTGTACGGAGTAGGACAAATCGGCCGGGAGCGGAGTGTAACGTTGCTAAGCAAGGGCCCGGAGGGTGGCGGGCAGG‑AC‑GCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATg < 2:702036/301‑1 (MQ=21) aaaaCGAGAGCGTCAGTGGAGAGCCTGGGCGTTTGGTTGTATCTGTTGTTGGTCGGTGAACGGTCGCCGGAGAGGGAAAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑AC‑GCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAA‑c < 1:773469/301‑1 (MQ=21) aagAAAGGCTCGGTAGAAAGCCTGGGCGTGTGTTGTTATCTGTGGGTTGGAGGTGAACGAGCTCCTGAGTAGGAAAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑AC‑GCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAA‑CCAc < 2:1103099/299‑1 (MQ=21) aaggcGAAAGACTGGGCCTTTCGTTTTACCTGTTGTTTGTCGGTGAACGCTCTCCGGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑AC‑GCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAA‑CCACTCCTTAAAAc < 2:1019351/297‑1 (MQ=255) aggggAAAGGCGGGGGCTTTCGTTTTATGTGTTGTTGGGGGGGGAACGCTCTGCTGGGTGGGACAAAGCGGCCGGGAGCGGATTTGAACGTGGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑AC‑GCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAA‑CCACTCCTTAAAAc < 1:57105/296‑1 (MQ=21) gTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCGGG‑AC‑GCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTCCAAACTCTTCCTTTCGTCCTATCTACAAGCCATCCCCCCACAGATACGGTACACTAGCCTCGTTTTTGCATCAGGAACGCAGCAATGAA‑CCCATCCTGAAAccc > 2:760565/1‑300 (MQ=18) tCGAAAGGCTGGGCCTGTCGTTTTACCTGTTGTGTGTCGGGGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGCATTTGAACGTGGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑AC‑GCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAA‑CCACTCCTTAAAAc < 2:1140052/298‑1 (MQ=255) gcGAAAGACGGGGCCTTGCGTGTTATCTGTTGTGTGTGGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCGGG‑AC‑GCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAA‑CCACTCCTTAAAAc < 2:92064/297‑1 (MQ=255) ctcatagccTGGGTCTTTTGTTTTATCTGTTGTGTGTCGGTAAACGCTCGCCTGAGTAGGACAAATCCGCCGGTAGCTGATTTGAACGTTGCGAAGCAACGGCCCGGATGGTGTCGGGCAGG‑AC‑GCCCGCCATAAACGGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAA‑CCACTCCTTAAAAc < 2:515639/290‑1 (MQ=21) acgggccagttctgttgttttgttttTTGCCGGTGAAAGCCCGTCTGAGGAGGAAATATCCGCCGGGAGCGTAGTTGAACGTTGGGAAGAAAGGGCACGGGGGGTGGCGGGCAGG‑AC‑GCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCAGCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAA‑CCACTCCTTAAAACCCTGGAac < 2:427143/279‑1 (MQ=25) aCTGGGCCTTTCGTTTTATCTGTGGTTAGTCGGTGAACGCTCTCCTGAGGAGGACAAATCGGCGGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑AC‑GCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAA‑CCACTCCTTAAAACCCTGGAac < 2:358424/299‑1 (MQ=255) tttttttgtcttggcggtttgtGGCTGAACGCCCCCCTGGGTAGGGACAGTCGCGCGCGCGCGGGCTTGACCGTTGGGAAGCAACCGCCGGGAGGG‑GGCCGGCAAG‑TCACCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACACACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTACACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAA‑CCACTCCTTAAAACCCTGGAACACAGTTGGCa < 1:1168354/285‑1 (MQ=25) tttgggtttATGTGTTGTTGGGCGGGGAAAGCGTGCCTGGGTAGCACAAAGCCGCCGGGAGGGGATTTGAAAGTGGCGAACCAACGGCCCGGAGGGGGGGGGGCAGG‑GC‑GCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAA‑CCACTCCTTAAAACCCTGGAACACATTTGGc < 1:17829/294‑1 (MQ=25) gTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCGGG‑AC‑GCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGcc > 1:743552/1‑207 (MQ=255) gTTTTAGCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCGGG‑AC‑GCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGcc < 2:743561/207‑1 (MQ=255) tttgtgtgggggtgaacgccgccgggggaaGGAAAAAGCGGCGGGGGGCGGAGTTGAACGTTGCGAAGCAAAGGCCCGGAGGGTGGCGGGCAGG‑AC‑GCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAA‑CCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCAT‑AATGc < 1:224634/272‑1 (MQ=25) tgtgtttgttGGTGAACGCTCTCCTGAGTAGGACAAATCCGGCGGGAGCCGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑AC‑GCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAA‑CCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCAT‑AATGc < 2:661042/299‑1 (MQ=255) gttgttTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑AC‑GCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAagcagc > 2:1169770/1‑250 (MQ=255) gtgttgtgtCGGTGAACGCTCTCATGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCGACGGCCCGGAGGGTGGCGGGCAGG‑AC‑GCCCGCCATAAACTGCCAGGCAGCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAA‑CCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCAT‑AATg < 2:252397/294‑1 (MQ=255) tggaaaaggagcggtcGCTCTCCGTGGGAGGACAAATCCGGCGGGATCTGATTTGAACGTTGCGAAGAAACGGCCCGGAGGGTGGGGGTCAGG‑AC‑GCACGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTGTTGCATCAGGAAAGCAGCTATGAA‑CCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCAT‑AATGc < 1:1514242/285‑1 (MQ=25) gtttgtCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑AC‑GCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAACCCATCCCCCCCCAGCTCCGGTAAACTAGCCGCGTTTTTGCATCAGGAACGCACCTATGAC‑GCACTCCTCAAAACCCTGTAACACATTTGGCATTAATCAT‑AATTCTCa > 1:1057644/1‑301 (MQ=21) gtCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑AC‑GCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGCTGGCCTCTTTGCGTTTCTACAAACTCGTACTGTCGTCATCTCTACAAGCCATCCCCCCACAGATACAGTAAACTAGCCTTGTTTTTGCACCAGGAAAGCAGCTCAGAA‑CCACTCCTTAAAACCATGGAACACAATTTGTAATGATCAA‑CATGCTCCGca > 2:571709/1‑300 (MQ=21) gcGGTGAACGCTCCCCGGAGGAGGACAGAACCGACGGGGGCGGAGTTGAACGTTGCGAAGCACCGGCCCGGCGGGTGGCGGTTAGG‑AC‑GCCCGCCATAAACTGACAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTGTTTGCGTTTCTACGAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAG‑CCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCAT‑AATGCTCAGcac < 1:53871/299‑1 (MQ=37) ggTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑AC‑GCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGCCGGGTGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTACCATAGCCTCGTTTTTGCATCAGGAAAGCAGCTAGGCA‑CCACTCCTTCAACCCCTGCACACCATTTGGCATTGATACT‑AATGCTCAACACCtt > 2:1209782/1‑301 (MQ=21) gTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑AC‑GCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACACACTCTTCCTGTCGCCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAA‑CCACCACTTAAAACCCTGGAACACATTTGGCATTGATc > 2:490868/1‑283 (MQ=255) aCGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGGGGCGGGCAGG‑AC‑GCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGCAGGCCATCCTGACGGCTGGCCTTTTTGCGGTTCTACAAACTCTTCCTGTCGACATATCTAAAAACCATCCCCCCACAGATAAGTTCAATTAGCCTCGTTTTTTCATCAGGAAAGCAGCTATGAA‑CAACTACTTCAAACCCTTGAACACATTTGGCATGGATCAT‑AATgactgtctctaatact > 2:1514286/1‑285 (MQ=21) ctccCCTGAGTAGGACAAATCCGCCGGCAGCGGATGTGAACGTTGCGAAGCAACGGCACGGAGGGTGGCGGGCGGG‑AC‑GCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAA‑CCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCAT‑AATGCTCAGCACATTGTATGTGc < 2:956883/301‑1 (MQ=255) ctcCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑AC‑GCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACCGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAC‑CCACTCCTTAAAACCCTCGAACACATTTAGCATTGATCAT‑AATGCTCAGCACATTGTATGTGCCg > 2:480071/1‑301 (MQ=255) ctcCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑AC‑GCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAACGCAGCTATGAA‑CCACTCCtt > 1:263461/1‑245 (MQ=255) ctcCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑AC‑GCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTAAAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCTTCAGGAAAGCAGCTATGAA‑CAACTCCCTAAAACCCTGGAACCAATTTGGCCTTGATCAT‑CACGTTTAGCAAATTgatattgccg > 2:1374493/1‑292 (MQ=21) ctcCGGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑AC‑GCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAA‑CCACTCCtt < 2:263467/245‑1 (MQ=255) ccTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑AC‑GCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCCATGAA‑CCCCCCCTTAAAACCCTGGAacac > 1:587226/1‑258 (MQ=255) ggggtgggACAAAGCCGCGGGGGGCGGATTTGAACGTTGGAAAGCAACGGCCCGGGGGGGGGCGGGCAGG‑CC‑GCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAA‑CCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCAT‑AATGCTCAGCACATTGTATGTGCCGAAg < 1:333896/294‑1 (MQ=38) gAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑AC‑GCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTCCAAACGCTTCCTGTCGTCATATCTACAAGCCACCCCCCCACAGATACGGTCCACTCGCCTCGTTTTTGACTCAGGAAAGCAGCTATGAA‑CCACTCCTGAACACCCTTGAACACATTTGTCCTCTCTCCA‑CATGCTCCTCACATTGTCTCTGCCGCAGaa > 1:343616/1‑300 (MQ=18) ggTAGGAAAAAGCCTGCGGGGGCGGAATTGAAAGTTGGGAAGCAAGGGCCGGGAGGGGGGCGGGCAGG‑AA‑GCCCGCCATAAACTGCCAGTCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTGCAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAA‑CCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCAT‑AATGCTCAGCACATTGTATGTGCCGAAGAc < 1:529861/299‑1 (MQ=37) ggACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑AC‑GCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCCTCCCCCCACAGATCCGTTAAACTAGCCTCGTTTTTGCATCAGGGAAGCAGCTATGAA‑CCACTCCTTAAAAc > 2:1343707/1‑240 (MQ=255) cgcggtggCGGATTGGAACGTTGGTAAGTAACGTCCAGGGGGGTGGTGGGCAGT‑AC‑CCCCGCTATAAACTGACAGACATCAAATTAAGAAGAAGGCCATACTGACGGATGGCCTTTTTGCGTGTCTACAAACTCTGCCTGTCGTCATATCTACAATCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCATCTATGAA‑CCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCAT‑AATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTc < 2:787415/294‑1 (MQ=255) cccgGGATCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGTGTGGCGGGCAGG‑AC‑GCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAA‑CCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCAT‑AATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTc < 2:1516010/300‑1 (MQ=255) aGCGGATTTGAAGGTTGCGAAGAAACGGCCCGGAGGGGGGCGGGCAGG‑AC‑GCCCGCAATAAACGGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAA‑CCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCAT‑AATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGc < 2:796959/300‑1 (MQ=255) gCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑AC‑GCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAA‑CCACTCCTTAAAACCCTGGACAACATTTGGCATTTATCAT‑AATGCTCAGCACATTCAATGTGCCGACTACGACCAACAATTACTCAAtcc > 2:767541/1‑297 (MQ=255) gATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑AC‑GCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACCAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAA‑CACATATAAACCCACCCCTT‑AAACCCTTGGCCACATTTTGCCTTGAACATAAATGCCTACCAAATT‑TTTGTGCCCAAGACGAAAAAAAAATACCCAAAGCCCGGCa > 1:30605/1‑301 (MQ=21) tgtGAAAGTGGCGAAGAAACGGCCCGGAGGG‑GGCCGGCGGGTAG‑GACCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCATGACGTATGGCCGTTTTGCGTTCCTACAAACTCTTCCTGTCGTCATATCTACAAGACATCCCCCCACAGAGACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAA‑CCACTCCTTAAAACCCGGGAACACATGTGGCATTGATCAT‑AATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAg < 2:349668/299‑1 (MQ=255) acgggtgcgagcaaagcgccGGAGGGTGTCGGTAAGG‑AC‑TCCCGCAATAAACTGCCAGTCATCAAAGTAAGCACAAGGCCACCCTGACTGATGGCCTTTTGGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCACCCCCCCGCAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAA‑CCACTCCTTAAAACCCTGGAACACAGTTGGCATTGATCAT‑AATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATCACTCAATGCCTGGCAGTATGc < 1:52449/283‑1 (MQ=255) gtggggAAGGAACGGCCCGGGGGGTTGGGGGCCGG‑AC‑GCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAA‑CCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCAT‑AATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCa < 1:791870/295‑1 (MQ=255) ttggaaaGCAACGCGACGGAGGGTGGCGGGCAGG‑AC‑GCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAA‑CCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCAT‑AATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAAc < 2:322901/296‑1 (MQ=255) cAACGGCCCGGAGGGTGGCGGGCAGG‑AC‑GCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAA‑CCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCAT‑AATGCTCAGcac > 2:1247769/1‑240 (MQ=255) accGGCCCGGAGGGTGGCAGGCAGG‑AC‑CCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCGTACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAA‑CCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCAT‑AATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAACCGCAGAAgg < 1:1374453/299‑1 (MQ=255) ggCCCGGAGGGTGGCGGGCAGG‑AC‑GCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAA‑CCACTCCTTAAAACCCTGGAACACCTTTGGCATTGATCCT‑AATGCTAAGCAAATTGt > 2:1327422/1‑241 (MQ=255) gggCAGG‑AC‑GCCCGCCATAAACGTCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGGTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCCCAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAA‑CCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCAT‑AATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAACCGCAGAAGGACAACCCGTTCTTATTc < 1:767532/300‑1 (MQ=255) gggAAGG‑AC‑TCCCGAAATAAACTGCCAGTGATCAAATTAAGCACAAGGCAATCCTGAAGGATGGCCTTTTTGCGTTTCCACAAACGCTTCCTGTCGTCATGTCTACCAGCCATCCCCCCACAGAGACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAA‑CCACTCCTTAAATCCCTGGATCACATTTGGCATTGATCAT‑AATGCTCAGCACATTGTATGTGCCGAAGACGAACAAAAATTACTCAATGCCTGGCAGTATGCAACCGCAGAAGGACAACCCGTTCTTATTc < 2:790022/300‑1 (MQ=255) | ACGCAGAAGCGGTCTGATAAAACAGAATTTGCCTGGCGGCAGTAGCGCGGTGGTCCCACCTGACCCCATGCCGAACTCAGAAGTGAAACGCCGTAGCGCCGATGGTAGTGTGGGGTCTCCCCATGCGAGAGTAGGGAACTGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCCTGAGTAGGACAAATCCGCCGGGAGCGGATTTGAACGTTGCGAAGCAACGGCCCGGAGGGTGGCGGGCAGG‑AC‑GCCCGCCATAAACTGCCAGGCATCAAATTAAGCAGAAGGCCATCCTGACGGATGGCCTTTTTGCGTTTCTACAAACTCTTCCTGTCGTCATATCTACAAGCCATCCCCCCACAGATACGGTAAACTAGCCTCGTTTTTGCATCAGGAAAGCAGCTATGAA‑CCACTCCTTAAAACCCTGGAACACATTTGGCATTGATCAT‑AATGCTCAGCACATTGTATGTGCCGAAGACGAACAACAATTACTCAATGCCTGGCAGTATGCAACCGCAGAAGGACAACCCGTTCTTATTCTGG > NC_000913/4171608‑4172195 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |