| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,685,757 | G→A | H278Y (CAC→TAC) | fumC ← | fumarate hydratase (fumarase C),aerobic Class II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,685,757 | 0 | G | A | 98.5% | 455.9 / NA | 135 | H278Y (CAC→TAC) | fumC | fumarate hydratase (fumarase C),aerobic Class II |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (65/68); minor base C (2/0); total (67/68) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 135 total aligned reads displayed.
CAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTCCGCTACGTGAGGCAGGCTGTATTCGATATGTTTGAGATTATGCTCGAGCATCGCTACCCAGCCGGAAATCTCCTGCCCCAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGG > NC_000913/1685464‑1686053 | cAAGCGCACCGGTTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGcc < 1:1018916/298‑1 (MQ=255) aaGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGaa < 1:1219/301‑1 (MQ=255) aGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGaa > 1:871073/1‑300 (MQ=255) gCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCCGCGGACATCATTGGCCATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTCCGCCTTAACCAGGGCATccc > 2:723778/1‑298 (MQ=255) gCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGCTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTCCACCTGAACCAGGGCATcac > 1:1053557/1‑300 (MQ=255) gtgGATAACCAGTGGACTGAAGACGTTGAGTTCAAAGTGCCCGGAAGCGCCCCCCATGTTGAGCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAg < 2:769806/300‑1 (MQ=255) tgGATCACCATTGGACGGAAGACGTTCAGTACAAAGTTACCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGGTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAgcg < 2:982136/301‑1 (MQ=255) ggATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGCACCAGGGCATCCCAGGTCGCCAccg > 2:18173/1‑300 (MQ=255) agcACCATGGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCt < 2:1027824/298‑1 (MQ=255) tGGACGGAAGACGTTCAGTTGAAAGTTACCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTg < 1:407958/301‑1 (MQ=255) tGGACGGAAGACGTTCAGTTCAAAGTTACCGGCAGCTCCCCCCATGTTGATCGACACGTCGTTCCCCAGCACCTGACAGCAGGGCATGGTTAATGCCTCACACTGTGTTGGGTTCACGTTCCACGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCGCAGGTCGCCAGCGCTTCAAATTTg < 2:1131469/301‑1 (MQ=255) ctcttacgatctgttcagttcaAAGTTACCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAAttt < 2:72937/288‑1 (MQ=255) gAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCCGTGACGCAGCCAACCCTTTCAAC‑GCCCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCttagatcggaagagcg > 2:82987/1‑287 (MQ=255) aCGTTCAGTTCAAAGTTACCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCgg < 1:298613/301‑1 (MQ=255) tcaAAGTTACCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCCGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACg > 1:541170/1‑301 (MQ=255) tcttccgatcgccccATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAgg < 2:1120035/290‑1 (MQ=255) cGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGGTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCAAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCTCGTCCAATTTGTTCGGCGCGGTAACAAACGGTGCCCAggg > 2:75090/1‑300 (MQ=255) ggAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTa > 1:277834/1‑301 (MQ=255) aGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATg > 1:1039866/1‑301 (MQ=255) aGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGCCGCGGTAACAAACGGTGCACAGGTAATg > 2:650339/1‑301 (MQ=255) aGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTACGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCCGAACCACGGCATCCCAGGTCGACAGCGATTCAAATTTGTTCGGCGCGGTAACACACGGTTCACACGCAATg > 2:198403/1‑301 (MQ=255) cgcCCCCCATGTTGAGCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGTGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATg < 2:265004/299‑1 (MQ=255) ccccATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAggagatcggaaga > 1:1121464/1‑290 (MQ=255) tGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGTGCTGCCCGGCTCATTTTCCGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTc < 1:642919/301‑1 (MQ=255) gATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCACC‑GCGCCCTACGCCTGAACCAGGGCAACCCCGGGCGCCAGCGCTTCAAATTTGTTCGGCCCGGTAACAAACGGTGCCCAGGTAATGACTGCCAGGTCATc > 2:317622/1‑300 (MQ=255) gATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCTGTACGCCTGAACCAGGGCATCACAGGTCGGCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTCAACAGGTAATGGCTGCCCGTTGAttt > 1:64877/1‑299 (MQ=255) aCGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTacgc < 1:724575/301‑1 (MQ=255) cgtcgtTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGACTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTacgcg > 1:1007850/1‑301 (MQ=255) cgtTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCAacgcgacg > 1:819675/1‑301 (MQ=255) ttCCACATCGCCTGACAGCAGAGAATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTacgcgacgcg < 1:126033/301‑1 (MQ=255) tCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAATGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCCCAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGACAGTTCCTCTGCTacgcgacgcg > 1:1010432/1‑300 (MQ=255) ccccAGCACCGACCAGCAGAGCATGGTTAATGCCTCACCCTGTGTGGTGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATGTTCCGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCa < 2:818741/301‑1 (MQ=255) cccATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCAt < 1:805532/301‑1 (MQ=255) aCCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTCACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCg > 2:781074/1‑301 (MQ=255) aCCTGACAGCAGAGCAGGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAAGTCGCCAGCGCTTCACATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTACTGACTGCCGGTTACGCTGCTACGCGACGCGCCTACTCCg > 2:559957/1‑301 (MQ=255) tGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCCCAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCCCATACTCCgt > 2:753594/1‑298 (MQ=255) tGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGAt < 2:431292/301‑1 (MQ=255) cagAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATggg < 1:18174/298‑1 (MQ=255) cagAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATg < 1:651050/296‑1 (MQ=255) cagAGCATGGTTAATGCCGCACACTGTTTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATggg < 1:131645/298‑1 (MQ=255) cagAGCATGGTTAATGCCCCACACTGTGTTTGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGAGTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATggg < 2:335134/298‑1 (MQ=255) agCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTAtt < 1:754439/299‑1 (MQ=255) agCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGACGCCAGCCAGCGGCCATCATTGGCGCTTTTCATCAGTCACGAAGCCAACCCTTTCAAC‑GCGCCTTACGCCTGAACCAGGGCATCACAGGCCGCCAGCGCTTCACATTTTTTCGGCGCGGTAACACACGGTGCCCTGGTAATGCCTTACCGTTCATCTGCTACGCTACGCTCATACttccgctggttcattt > 2:716141/1‑286 (MQ=255) tGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTc > 1:1147477/1‑300 (MQ=255) gCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCCACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACCGGACGCGCATACTCCGGagatcggaggagcgtcgtgtagg > 2:539936/1‑279 (MQ=255) ccTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGA‑TTTCACCAATTACGCAGCGCGCGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGCTTCACAGGTAATGACTGCCCGTTCATCTGCTACGCTACGCGCATACTCCGGATGGGTCTTTAGTCCAGTACCCa > 1:18302/1‑300 (MQ=255) tgtTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTAcc > 1:1077204/1‑300 (MQ=255) tgtTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCg > 1:776469/1‑301 (MQ=255) gtTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCg > 1:851580/1‑300 (MQ=255) tGGGTTCACTTTCACCGGCATGATTGAGCTGCCCGGCTCATGTGCCGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTGCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCa < 2:870016/301‑1 (MQ=255) ggTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAAACCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAag > 1:117966/1‑301 (MQ=255) tttCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAaga < 1:1137469/300‑1 (MQ=255) gTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAaga > 1:166374/1‑301 (MQ=255) gTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGAGTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAag < 2:886002/300‑1 (MQ=255) cccGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTCCg > 1:652472/1‑301 (MQ=255) cATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTCCGCTACg < 2:963455/301‑1 (MQ=255) aTTGAGCTGCCCGGCTCATTTTCCGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGATTGCCAGTTCATCTGCTACGCGACGCGCATCCTCCGGATGGGTATTTAGTCCAGTACCCACCCCTGTACCGCCAAGAGCCAGTTCCCCTACGTTag > 1:956354/1‑301 (MQ=255) aTTGAGCTGCCCGGCTCATTTTCCGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTCCGCTACGTGag > 1:980282/1‑301 (MQ=255) taaGCTGCCCGGCTCATGTTCCGGGATTGAGAGTTGCACC‑ATTCCGCAGCGCGGGCCAGCGGCCAGCCAGCGGACATCATTGGCGATTGTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTCCGCTACGTGaggc < 2:540559/299‑1 (MQ=255) tGAGCTGCCCGGCTCATTTTCCGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTCCGCTACGTGagg > 1:1119024/1‑300 (MQ=255) cTGCCCGGCTCATTTTCCGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTCCGCTACGTGaggcaggc < 1:321405/301‑1 (MQ=255) tGCCCGGCTCATTTTCCGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTCCGCTACGTGaggcaggc > 1:867202/1‑300 (MQ=255) cccGGCTCATGTTCCGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTCCGCTACGTGAGGCAGGCTGt < 1:824466/301‑1 (MQ=255) ccggaaaatgagccGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACCCCGGATGGGTATTTAGTCCAGTACCCACCGCTTTACCCCCCAGACCCAGTTCCGCTACGTGAGGCAGGCTgga > 2:1183072/13‑299 (MQ=255) cgatctttCCGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTCCGCTACGTGAGGCAGGCTGTATTCg < 1:700102/296‑1 (MQ=255) cTCATTTTCCGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTCCGCTACGTGAGGCAGGCTGTATTCg < 1:964646/301‑1 (MQ=255) tCATTTTCCGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTCCGCTACGTGAGGCAGGCTGTATTCGa > 1:522438/1‑301 (MQ=255) ttCCGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGCACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGTCTACTCCGGATGGGTATTTAGTCCATTACCCACCGCTGTACCGCCAAGCGCCTGTTCCGCTACGTGTGGCAGGGCGTATCCGagatcg > 2:699305/1‑299 (MQ=255) cGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTCCGCTACGTGAGGCAGGCTGTATTCGATATGTTTg < 1:75155/301‑1 (MQ=255) gggattggga‑ttTCACCAATCCCCCAGGGCGGGCCAAAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTCCGCTACGTGAGGCAGGCTGTATTCGATATGTTTg < 2:166209/300‑1 (MQ=255) aTTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGATGGCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTAGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTCCGCTACGTGAGGCAGGCTGTATTCGATATGTTTGAGAt < 1:964540/301‑1 (MQ=255) tGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTCCGCTACGTGAGGCAGGCTGTATTCGATATGTTTGAGATTa < 2:521861/301‑1 (MQ=255) cccttccgatctCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTCCGCTACGTGAGGCAGGCTGTATTCGATATGTTTGAGATTATGCt < 2:225270/290‑1 (MQ=255) tggaaacAATTCCGCAGCGCGGGACAGAGGGCAGCCAGCGGACATCATTGGCGAGTTTCATCAGTGACGCAGCCAACCCTTGCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTGCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTCCGCTACGTGAGGCAGGCTGTATTCGATATGTTTGAGATTATGCTc < 2:829301/295‑1 (MQ=255) cACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTCCGCTACGTGAGGCAGGCTGCATTCGATATGTTTGAGATTATGCTCgtg > 2:200790/1‑299 (MQ=255) ttCCGCAGGGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTCCGCTACGTGAGGCAGGCTGTATTCGATATGTTTGAGATTATGCTCGAGCATCGc < 1:716938/301‑1 (MQ=255) tCCGCAGCGCGGGCCAGAGGGCAGCCAGAGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGGGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTCCGCTACGTGAGGCAGGCTGTATTCGATATGTTTGAGATTATGCTCGAGCATCGc < 2:800737/300‑1 (MQ=255) ccGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTCCGCTACGTGAGGCAGGCTGTATTCGATATGTTTGAGATTATGCTAGatcggaagag > 1:225520/1‑292 (MQ=255) cgcgGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTCCGCTACGTGAGGCAGGCTGTATTCGATATGTTTGAGATTATGCTCGAGCATCGCTACCCAg > 1:931416/1‑300 (MQ=255) gCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTCCGCTACGTGAGGCAGGCTGTATTCGATATGTTTGAGATTATGCTCGAGCATCGCTACCCAGCCGGaa > 1:942650/1‑301 (MQ=255) agccagccaGCGGACCTCATTGGCGATTTTCATCAGTGACGCAGCCAACCCCGTCAAC‑GCGCCGTACGCCTGTACCCGGCCATCACAGGTCGCAAGCGCTTCAAATTTGTTCGTCGAGGTAACAAACGGAGCACAGGTAATGACCGCCCGTTCATCTGCTACGCGACGCGCATACCACGGATGGGTATTTAGTCCAGTACCCACCGCTATAGTGACAAGAGCCCTTTTCGCTACGTTAGGCAGGCCGTAGTCGATATGGTTGAGTTTCTGAGCGAGGATCGGTCCCCAAGCTGAAAttcc > 2:645622/2‑297 (MQ=255) ccagccagCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGTTATTTAGTCCAGTACCCACCGCTGTACCGCCCAGAGCCCGTTCCGCTACGTGAGGCAGGCTGTATTCGATATGTTTGATATTATGCTCGACCATCGCTACCCAGCCGGCACTCTCCtt > 1:503612/1‑300 (MQ=255) ccagccagCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTCCGCTACGTGAGGCAGGCTGTATTCGATATGTTTGAGATTATGCTCGAGCATCGCTACCCAGCCGGAAATCTCCt < 1:781963/300‑1 (MQ=255) ccagcGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTCCGCTACGTGAGGCAGGCTGTATTCGATATGTTTGAGATTATGCTCGAGCATCGCTACCCAGCCGGAAATCTCCTGcc < 2:709626/299‑1 (MQ=255) cGGACATCATTGGCGATTTTCAACAGTGACGCCGCAAACCCTTTCACA‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCAAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTCCGCTACGTGAGGCAGGCTGTATTCGATATGTTTGAGATTATGCTCGAGCATCGCTACCCAGCCGGAAATCTCCTGCCCCAGc < 2:859862/300‑1 (MQ=255) tcatTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTCCGCTACGTGAGGCAGGCTGTATTCGATATGTTTGAGATTATGCTAGAGCATCGCTACCCAGCCGGAAATCCCCTGCCCCAGCGTTAACg > 2:450245/1‑301 (MQ=255) cGATTTTCTTCATGGACCAAGACATCCCTTGCAAC‑GCGACATACGCCTGACCCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGGTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCAACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCCCTGTACCGCCAAGAGCCAGTTCCGCTACGTGAGGCAGGCTGTATTCGATATGTTTGAGATTATGCTCGAGCATCGCTACCCAGCCGGAAATCTCCTGCCCCAGCGTTAACGGCGTgg < 2:1038554/300‑1 (MQ=255) aTTTTCATCAGTGACGCAGCCAACCCGTTCAAC‑GCGCAGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCGACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTCCGCTACGTGAGGCAGGCTGTATTCGATATGTTTGAGATTATGCTCGAGCATCGCTACCCAGCCGGAAATCTCCTGCCCCAGCGTTAACGGCGTGGCAt < 2:775580/301‑1 (MQ=255) tttaatcacggccGCAGGCAACCCTTTCCAC‑GCGCCGTACGCCTGAACCAGGGCATCCATGGTCGCCAGCGCTTCAAATTTGTGCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTCCGCTACGTGAGGCAGGCTGTATTCGATATGTTTGAGATTATGCTCGAGCATCGCTACCCAGCCGGAAATCTCCTGCCCCAGCGTTAACGGCGTGGCATcc < 2:277552/289‑1 (MQ=255) atcaGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTCCGCTACGTGAGGCAGGCTGTATTCGATATGTTTGAGATTATGCTCGAGCATCGCTACCCAGCCGGAAATCTCCTGCCCCAGCGTTAACGGCGTGGCATCCTGCa > 1:908339/1‑301 (MQ=255) agcggaagcAAACCTTTTAACAGCGCCGTACGCCGTACCCATGGCATCACGGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCCCCGCTGTACCGCCAAGAGCCAGTTCCGCTACGTGAGGCAGGCTGTATTCGATATGTTTGAGATTATGCTCGAGCATCGCTACCCAGCCGGAAATCTCCTGCCCCAGCGTTAACGGCGTGGCATCCTGCAAg < 2:1006595/290‑1 (MQ=255) acccgaccAACCCTTTCAAC‑TCGCCGTAAGCCTGAACCCGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTCCGCTACGTGAGGCAGGCTGTATTCGATATGTTTGAGATTATGCTCGAGCATCGCTACCCAGCCGGAAATCTCCTGCCCCAGCGTTAACGGCGTGGCATCCTGCAAGTGAg < 2:1128878/294‑1 (MQ=255) aGCCAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTCCGCCACGTGAGGCAGGCGGTATTCGATATGTTTGAGATTATTCTCGAGCATCGCTCCCCAGTCGGAAATCTCCTGCCCCAGCGTTAACGTCGTGGCCTCTCGTAAGTTAGTACGa > 2:1007031/1‑301 (MQ=255) ccAACCCTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTCCGCTACGTGAGGCAGGCTGTATTCGATATGTTTGAGATTATGCTCGAGCATCGCTACCCAGCCGGAAATCTCCTGCCCCAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACg < 1:715956/298‑1 (MQ=255) cccTTTCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTCCGCTACGTGAGGCAGGCTGTATTCGATATGTTTGAGATTATGCTCGAGCATCGCTACCCAGCCGGAAATCTCCTGCCCCAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAAtt > 1:527989/1‑301 (MQ=255) tttCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTCCGCTACGTGAGGCAGGCTGTATTCGATATGTTTGAGATTATGCTCGAGCATCGCTACCCAGCCGGAAATCTCCTGCCCCAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTg > 1:1029748/1‑301 (MQ=255) tttCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTCCGCTACGTGAGGCAGGCTGTATTCGATATGTTTGAGATTATGCTCGAGCATCGCTACCCAGCCGGAAATCTCCTGCCCCAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTg < 1:243305/301‑1 (MQ=255) tCAAC‑GCGCCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTCCGCTACGTGAGGCAGGCTGTATTCGATATGTTTGAGATTATGCTCGAGCATCGCTACCCAGCCGGAAATCTCCTGCCCCAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGAc < 1:307330/301‑1 (MQ=255) gcgcCGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTCCGCTACGTGAGGCAGGCTGTATTCGATATGTTTGAGATTATGCTCGAGCATCGCTACCCAGCCGGAAATCTCCTGCCCCAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGatat > 1:778099/1‑301 (MQ=255) ccGTACGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTCCGCTACGTGAGGCAGGCTGTATTCGATATGTTTGAGATTATGCTCGAGCATCGCTACCCAGCCGGAAATCTCCTGCCCCAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCgg < 1:450757/301‑1 (MQ=255) | CAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCGCCCCCCATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGA‑TTTCACCAATTCCGCAGCGCGGGCCAGAGGCCAGCCAGCGGACATCATTGGCGATTTTCATCAGTGACGCAGCCAACCCTTTCAAC‑GCGCCGTGCGCCTGAACCAGGGCATCACAGGTCGCCAGCGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTCCGCTACGTGAGGCAGGCTGTATTCGATATGTTTGAGATTATGCTCGAGCATCGCTACCCAGCCGGAAATCTCCTGCCCCAGCGTTAACGGCGTGGCATCCTGCAAGTGAGTACGACCAATTTTGACGATATCGG > NC_000913/1685464‑1686053 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |